RESUMO
Nitrogen (N) deficiency levels were investigated for their potential to maintain the yield and improve antioxidant activity of Coreopsis tinctoria. Inflorescences and leaves at 0, 10, 20, 30, 40, and 50 d after flowering were frozen at -80 °C and plant growth, antioxidant activity, bioactive substance, enzyme activity, and gene expression were evaluated. N deficiency maintained the total number of flowers, promoted phenol and flavonoid accumulation, and enhanced antioxidant activity. Moreover, N deficiency stimulated activities of phenylalanine ammonia-lyase (PAL), cinnamate-4-hydroxylase (C4H) and 4-coumarate:coenzyme A ligase (4CL), and induced CtPAL, CtC4H and Ct4CL gene expression. The data also suggest that N-deficiency-induced phenolic and flavonoid accumulation occurs due to the activation of biosynthetic pathways in C. tinctoria. We characterize the unique features of C. tinctoria under N-deficiency conditions and provide valuable information for the cultivation of high-N use efficiency varieties with low input and high output.
Assuntos
Antioxidantes/metabolismo , Coreopsis/crescimento & desenvolvimento , Coreopsis/metabolismo , Nitrogênio/deficiência , Flavonoides/metabolismo , Flores/crescimento & desenvolvimento , Fenol/metabolismoRESUMO
CONTEXT: The buds of Coreopsis tinctoria Nutt (Compositae) are used in the treatment of hypertension in the Uyghur folk medicine in China. OBJECTIVE: To investigate vasorelaxant properties of extracts and some flavonoids from C. tinctoria (CT) and their underlying mechanisms in isolated rat thoracic aortic rings. MATERIALS AND METHODS: Vasorelaxant effects of ethanol extracts of CT (CTA) and its flavonoids as well as water-ethanol eluates from CTA by AB-8 resins (CTAAâ¼CTAF) were evaluated on rat aortic rings pre-contracted with phenylephrine (PE, 1 µM) or high KCl (60 µM). We evaluated the effect of CTA, CTAD and CTAE on PE-induced contraction in a Ca²âº-free medium and a dose-effect curve of Ca²âº in pre-contracted ring with high KCl. RESULTS: Endothelial removal did not modify the effect of CTAD and CTAE (3.00 g/L) neither on PE-pre-contracted rings (164.78 ± 21.44 and 191.47 ± 16.75%) nor on KCl-pre-contracted rings (75.68 ± 10.76 and 125.14 ± 17.41%) compared with intact-endothelium rings pre-contracted with high KCl (100.49 ± 17.30 and 110.81 ± 16.33%). CTAD and CTAE (3.00 g/L) down-regulated the dose-effect curve of Ca²âº in pre-contraction with high KCl, and inhibited the pre-contraction with PE in a Ca²âº-free medium (p < 0.05). Seven flavonoids were obtained from CTAD, of which luteolin (5) and quercetin (6) were found to be the most effective relaxation in rings precontracted with PE (EC50: 0.006 and 0.039 g/L, p < 0.05) or high KCl (EC50: 0.023 and 0.045 g/L, p < 0.05). DISCUSSION AND CONCLUSION: These data demonstrated the vasorelaxant effect of CT, and its mechanism is likely due to an inhibitory effect on calcium movements through cell membranes.
Assuntos
Aorta Torácica/efeitos dos fármacos , Sinalização do Cálcio/efeitos dos fármacos , Coreopsis/química , Medicamentos de Ervas Chinesas/farmacologia , Flavonoides/farmacologia , Vasodilatação/efeitos dos fármacos , Vasodilatadores/farmacologia , Animais , Anti-Hipertensivos/química , Anti-Hipertensivos/isolamento & purificação , Anti-Hipertensivos/farmacologia , China , Coreopsis/crescimento & desenvolvimento , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Endotélio Vascular/fisiologia , Etnofarmacologia , Flavonoides/química , Flavonoides/isolamento & purificação , Flores/química , Flores/crescimento & desenvolvimento , Técnicas In Vitro , Luteolina/química , Luteolina/isolamento & purificação , Luteolina/farmacologia , Masculino , Fenilefrina/antagonistas & inibidores , Fenilefrina/farmacologia , Quercetina/química , Quercetina/isolamento & purificação , Quercetina/farmacologia , Ratos , Ratos Wistar , Vasoconstritores/antagonistas & inibidores , Vasoconstritores/farmacologia , Vasodilatadores/química , Vasodilatadores/isolamento & purificaçãoRESUMO
A greenhouse experiment was conducted to evaluate the potential role of arbuscular mycorrhizal fungi (AMF) in encouraging revegetation of copper (Cu) mine tailings. Two native plant species, Coreopsis drummondii and Pteris vittata, together with a turf grass, Lolium perenne and a leguminous plant Trifolium repens associated with and without AMF Glomus mosseae were grown in Cu mine tailings to assess mycorrhizal effects on plant growth, mineral nutrition and metal uptake. Results indicated that symbiotic associations were successfully established between G. mosseae and all plants tested, and mycorrhizal colonization markedly increased plant dry matter yield except for L. perenne. The beneficial impacts of mycorrhizal colonization on plant growth could be largely explained by both improved P nutrition and decreased shoot Cu, As and Cd concentrations. The experiment provided evidence for the potential use of local plant species in combination with AMF for ecological restoration of metalliferous mine tailings.