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1.
Arch Virol ; 163(4): 1117-1124, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29260329

RESUMO

Cystoviridae is a family of bacterial viruses (bacteriophages) with a tri-segmented dsRNA genome. It includes a single genus Cystovirus, which has presently only one recognised virus species, Pseudomonas virus phi6. However, a large number of additional dsRNA phages have been isolated from various environmental samples, indicating that such viruses are more widespread and abundant than previously recognised. Six of the additional dsRNA phage isolates (Pseudomonas phages phi8, phi12, phi13, phi2954, phiNN and phiYY) have been fully sequenced. They all infect Pseudomonas species, primarily plant pathogenic Pseudomonas syringae strains. Due to the notable genetic and structural similarities with Pseudomonas phage phi6, we propose that these viruses should be included into the Cystovirus genus (and consequently into the Cystoviridae family). Here, we present an updated taxonomy of the family Cystoviridae and give a short overview of the properties of the type member phi6 as well as the putative new members of the family.


Assuntos
Cystoviridae/genética , Genoma Viral , Filogenia , Pseudomonas/virologia , RNA de Cadeia Dupla/genética , RNA Viral/genética , Sequência de Bases , Cystoviridae/classificação , Cystoviridae/isolamento & purificação , Sequenciamento de Nucleotídeos em Larga Escala , Homologia de Sequência do Ácido Nucleico , Terminologia como Assunto
2.
J Gen Virol ; 96(Pt 5): 1180-1189, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25614591

RESUMO

Cystoviridae is a family of bacteriophages with a tri-segmented dsRNA genome enclosed in a tri-layered virion structure. Here, we present a new putative member of the Cystoviridae family, bacteriophage ϕNN. ϕNN was isolated from a Finnish lake in contrast to the previously identified cystoviruses, which originate from various legume samples collected in the USA. The nucleotide sequence of the virus reveals a strong genetic similarity (~80 % for the L-segments, ~55 % for the M-segments and ~84 % for the S-segments) to Pseudomonas phage ϕ6, the type member of the virus family. However, the relationship between ϕNN and other cystoviruses is more distant. In general, proteins located in the internal parts of the virion were more conserved than those exposed on the virion surface, a phenomenon previously reported among eukaryotic dsRNA viruses. Structural models of several putative ϕNN proteins propose that cystoviral structures are highly conserved.


Assuntos
Bacteriófagos/classificação , Bacteriófagos/isolamento & purificação , Cystoviridae/classificação , Cystoviridae/isolamento & purificação , Água Doce/virologia , Lagos/virologia , Bacteriófagos/genética , Análise por Conglomerados , Cystoviridae/genética , Finlândia , Dados de Sequência Molecular , Filogenia , Pseudomonas/virologia , RNA Viral/genética , Análise de Sequência de DNA , Homologia de Sequência do Ácido Nucleico
3.
BMC Microbiol ; 10: 55, 2010 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-20170499

RESUMO

BACKGROUND: Bacteriophage Phi12 is a member of the Cystoviridae and is distinct from Phi6, the first member of that family. We have recently isolated a number of related phages and five showed high similarity to Phi12 in the amino acid sequences of several proteins. Bacteriophage Phi2954 is a member of this group. RESULTS: Phi2954 was isolated from radish leaves and was found to have a genome of three segments of double-stranded RNA (dsRNA), placing it in the Cystoviridae. The base sequences for many of the genes and for the segment termini were similar but not identical to those of bacteriophage Phi12. However, the host specificity was for the type IV pili of Pseudomonas syringae HB10Y rather than for the rough LPS to which Phi12 attaches. Reverse genetics techniques enabled the production of infectious phage from cDNA copies of the genome. Phage were constructed with one, two or three genomic segments. Phage were also produced with altered transcriptional regulation. Although the pac sequences of Phi2954 show no similarity to those of Phi12, segment M of Phi2954 could be acquired by Phi12 resulting in a change of host specificity. CONCLUSIONS: We have isolated a new member of the bacteriophage family Cystoviridae and find that although it shows similarity to other members of the family, it has unique properties that help to elucidate viral strategies for genomic packaging and gene expression.


Assuntos
Cystoviridae/genética , Genoma Viral , RNA de Cadeia Dupla/genética , RNA Viral/genética , Sequência de Aminoácidos , Sequência de Bases , Mapeamento Cromossômico , Cystoviridae/química , Cystoviridae/isolamento & purificação , Eletroforese em Gel de Ágar , Engenharia Genética , Dados de Sequência Molecular , Mutação , Nucleocapsídeo/genética , Nucleocapsídeo/metabolismo , Folhas de Planta/virologia , Pseudomonas syringae/genética , Pseudomonas syringae/virologia , RNA de Cadeia Dupla/química , RNA Viral/química , RNA Viral/isolamento & purificação , Raphanus/virologia , Alinhamento de Sequência
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