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1.
Proc Natl Acad Sci U S A ; 113(16): 4314-9, 2016 Apr 19.
Artigo em Inglês | MEDLINE | ID: mdl-27044099

RESUMO

Lenz-Majewski syndrome (LMS) is a rare disease characterized by complex craniofacial, dental, cutaneous, and limb abnormalities combined with intellectual disability. Mutations in thePTDSS1gene coding one of the phosphatidylserine (PS) synthase enzymes, PSS1, were described as causative in LMS patients. Such mutations render PSS1 insensitive to feedback inhibition by PS levels. Here we show that expression of mutant PSS1 enzymes decreased phosphatidylinositol 4-phosphate (PI4P) levels both in the Golgi and the plasma membrane (PM) by activating the Sac1 phosphatase and altered PI4P cycling at the PM. Conversely, inhibitors of PI4KA, the enzyme that makes PI4P in the PM, blocked PS synthesis and reduced PS levels by 50% in normal cells. However, mutant PSS1 enzymes alleviated the PI4P dependence of PS synthesis. Oxysterol-binding protein-related protein 8, which was recently identified as a PI4P-PS exchanger between the ER and PM, showed PI4P-dependent membrane association that was significantly decreased by expression of PSS1 mutant enzymes. Our studies reveal that PS synthesis is tightly coupled to PI4P-dependent PS transport from the ER. Consequently, PSS1 mutations not only affect cellular PS levels and distribution but also lead to a more complex imbalance in lipid homeostasis by disturbing PI4P metabolism.


Assuntos
Anormalidades Múltiplas/enzimologia , Doenças do Desenvolvimento Ósseo/enzimologia , Membrana Celular/enzimologia , Retículo Endoplasmático/enzimologia , Complexo de Golgi/enzimologia , Deficiência Intelectual/enzimologia , Mutação , Transferases de Grupos Nitrogenados/metabolismo , Fosfatos de Fosfatidilinositol/metabolismo , Anormalidades Múltiplas/genética , Doenças do Desenvolvimento Ósseo/genética , Membrana Celular/genética , Retículo Endoplasmático/genética , Complexo de Golgi/genética , Células HEK293 , Humanos , Deficiência Intelectual/genética , Antígenos de Histocompatibilidade Menor , Transferases de Grupos Nitrogenados/genética , Fosfatos de Fosfatidilinositol/genética , Fosfotransferases (Aceptor do Grupo Álcool)/genética , Fosfotransferases (Aceptor do Grupo Álcool)/metabolismo
2.
Matrix Biol ; 47: 34-43, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-25957949

RESUMO

The ADAMTS (a disintegrin-like and metalloproteinase domain with thrombospondin-type 1 motifs) protein superfamily includes 19 secreted metalloproteases and 7 secreted ADAMTS-like (ADAMTSL) glycoproteins. The possibility of functional linkage between ADAMTS proteins and fibrillin microfibrils was first revealed by a human genetic consilience, in which mutations in ADAMTS10, ADAMTS17, ADAMTSL2 and ADAMTSL4 were found to phenocopy rare genetic disorders caused by mutations affecting fibrillin-1 (FBN1), the major microfibril component in adults. The manifestations of these ADAMTS gene disorders in humans and animals suggested that they participated in the structural and regulatory roles of microfibrils. Whereas two such disorders, Weill-Marchesani syndrome 1 and Weill-Marchesani-like syndrome involve proteases (ADAMTS10 and ADAMTS17, respectively), geleophysic dysplasia and isolated ectopia lentis in humans involve ADAMTSL2 and ADAMTSL4, respectively, which are not proteases. In addition to broadly similar dysmorphology, individuals affected by Weill-Marchesani syndrome 1, Weill-Marchesani-like syndrome or geleophysic dysplasia each show characteristic anomalies suggesting molecule-, tissue-, or context-specific functions for the respective ADAMTS proteins. Ectopia lentis occurs in each of these conditions except geleophysic dysplasia, and is due to a defect in the ciliary zonule, which is predominantly composed of FBN1 microfibrils. Together, this strongly suggests that ADAMTS proteins are involved either in microfibril assembly, stability, and anchorage, or the formation of function-specific supramolecular networks having microfibrils as their foundation. Here, the genetics and molecular biology of this subset of ADAMTS proteins is discussed from the perspective of how they might contribute to fully functional or function-specific microfibrils.


Assuntos
Proteínas ADAM/fisiologia , Microfibrilas/enzimologia , Animais , Doenças do Desenvolvimento Ósseo/enzimologia , Doenças do Desenvolvimento Ósseo/genética , Ectopia do Cristalino/enzimologia , Ectopia do Cristalino/genética , Fibrilina-1 , Fibrilinas , Humanos , Deformidades Congênitas dos Membros/enzimologia , Deformidades Congênitas dos Membros/genética , Síndrome de Marfan/enzimologia , Síndrome de Marfan/genética , Microfibrilas/metabolismo , Proteínas dos Microfilamentos/fisiologia
3.
Hum Genet ; 134(7): 691-704, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25893793

RESUMO

We describe a large family with disproportionate short stature and bone dysplasia from Nias in which we observed differences in severity when comparing the phenotypes of affected individuals from two remote branches. We conducted a linkage scan in the more severely affected family branch and determined a critical interval of 4.7 cM on chromosome 11. Sequencing of the primary candidate gene TBX10 did not reveal a disease-causing variant. When performing whole exome sequencing we noticed a homozygous missense variant in B3GAT3, c.419C>T [p.(Pro140Leu)]. B3GAT3 encodes ß-1,3-glucuronyltransferase-I (GlcAT-I). GlcAT-I catalyzes an initial step of proteoglycan synthesis and the mutation p. (Pro140Leu) lies within the donor substrate-binding subdomain of the catalytic domain. In contrast to the previously published mutation in B3GAT3, c.830G>A [p.(Arg277Gln)], no heart phenotype could be detected in our family. Functional studies revealed a markedly reduced GlcAT-I activity in lymphoblastoid cells from patients when compared to matched controls. Moreover, relative numbers of glycosaminoglycan (GAG) side chains were decreased in patient cells. We found that Pro140Leu-mutant GlcAT-I cannot efficiently transfer GlcA to the linker region trisaccharide. This failure results in a partial deficiency of both chondroitin sulfate and heparan sulfate chains. Since the phenotype of the Nias patients differs from the Larsen-like syndrome described for patients with mutation p.(Arg277Gln), we suggest mutation B3GAT3:p.(Pro140Leu) to cause a different type of GAG linkeropathy showing no involvement of the heart.


Assuntos
Doenças do Desenvolvimento Ósseo/genética , Doenças Genéticas Inatas/genética , Mutação de Sentido Incorreto , Adolescente , Adulto , Substituição de Aminoácidos , Doenças do Desenvolvimento Ósseo/enzimologia , Doenças do Desenvolvimento Ósseo/patologia , Criança , Pré-Escolar , Feminino , Doenças Genéticas Inatas/enzimologia , Doenças Genéticas Inatas/patologia , Glucuronosiltransferase/metabolismo , Humanos , Lactente , Masculino , Linhagem , Estrutura Terciária de Proteína
4.
Osteoarthritis Cartilage ; 23(7): 1214-20, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25707934

RESUMO

OBJECTIVE: In growth plate chondrocytes, loss of Dicer, a microRNA (miRNA)-processing enzyme, causes defects in proliferation and differentiation, leading to a lethal skeletal dysplasia. However roles of miRNAs in articular chondrocytes have not been defined in vivo. To investigate the role of miRNAs in articular chondrocytes and to explore the possibility of generating a novel mouse osteoarthritis (OA) model caused by intrinsic cellular dysfunction, we ablated Drosha, another essential enzyme for miRNA biogenesis, exclusively in articular chondrocytes of postnatal mice. DESIGN: First, to confirm that the essential role of miRNAs in skeletal development, we ablated the miRNA biogenesis pathway by deleting Drosha or DGCR8 in growth plate chondrocytes. Next, to investigate the role of miRNAs in articular cartilage, we deleted Drosha using Prg4-CreER(T) transgenic mice expressing a tamoxifen-activated Cre recombinase (CreER(T)) exclusively in articular chondrocytes. Tamoxifen was injected at postnatal days, 7, 14, 21, and 28 to ablate Drosha. RESULTS: Deletion of Drosha or DGCR8 in growth plate chondrocytes caused a lethal skeletal defect similar to that of Dicer deletion, confirming the essential role of miRNAs in normal skeletogenesis. Early postnatal Drosha deletion in articular chondrocytes significantly increased cell death and decreased Safranin-O staining. Mild OA-like changes, including surface erosion and cleft formation, were found in male mice at 6 months of age; however such changes in females were not observed even at 9 months of age. CONCLUSIONS: Early postnatal Drosha deficiency induces articular chondrocyte death and can cause a mild OA-like pathology.


Assuntos
Cartilagem Articular/patologia , Condrócitos/patologia , Osteoartrite/patologia , Ribonuclease III/fisiologia , Animais , Artrite Experimental/enzimologia , Artrite Experimental/genética , Artrite Experimental/patologia , Doenças do Desenvolvimento Ósseo/enzimologia , Doenças do Desenvolvimento Ósseo/genética , Doenças do Desenvolvimento Ósseo/patologia , Morte Celular/genética , Morte Celular/fisiologia , Feminino , Deleção de Genes , Lâmina de Crescimento/patologia , Masculino , Camundongos Knockout , Camundongos Transgênicos , MicroRNAs/genética , Osteoartrite/enzimologia , Osteoartrite/genética , Proteínas de Ligação a RNA/genética , Proteínas de Ligação a RNA/fisiologia , Ribonuclease III/deficiência , Ribonuclease III/genética , Tamoxifeno
5.
Biochim Biophys Acta ; 1851(6): 867-81, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25510381

RESUMO

More than twenty different genetic diseases have been described that are caused by mutations in phosphoinositide metabolizing enzymes, mostly in phosphoinositide phosphatases. Although generally ubiquitously expressed, mutations in these enzymes, which are mainly loss-of-function, result in tissue-restricted clinical manifestations through mechanisms that are not completely understood. Here we analyze selected disorders of phosphoinositide metabolism grouped according to the principle tissue affected: the nervous system, muscle, kidney, the osteoskeletal system, the eye, and the immune system. We will highlight what has been learnt so far from the study of these disorders about not only the cellular and molecular pathways that are involved or are governed by phosphoinositides, but also the many gaps that remain to be filled to gain a full understanding of the pathophysiological mechanisms underlying the clinical manifestations of this steadily growing class of diseases, most of which still remain orphan in terms of treatment. This article is part of a Special Issue entitled Phosphoinositides.


Assuntos
Doenças do Desenvolvimento Ósseo/genética , Neuropatia Hereditária Motora e Sensorial/genética , Deformidades Congênitas dos Membros/genética , Mutação , Miopatias Congênitas Estruturais/genética , Fosfatidilinositóis/metabolismo , Animais , Doenças do Desenvolvimento Ósseo/enzimologia , Doenças do Desenvolvimento Ósseo/patologia , Modelos Animais de Doenças , Expressão Gênica , Neuropatia Hereditária Motora e Sensorial/enzimologia , Neuropatia Hereditária Motora e Sensorial/patologia , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/genética , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Deformidades Congênitas dos Membros/enzimologia , Deformidades Congênitas dos Membros/patologia , Camundongos , Miopatias Congênitas Estruturais/enzimologia , Miopatias Congênitas Estruturais/patologia , Fosfatidilinositol 3-Quinases/genética , Fosfatidilinositol 3-Quinases/metabolismo , Monoéster Fosfórico Hidrolases/genética , Monoéster Fosfórico Hidrolases/metabolismo
6.
J Nutr Biochem ; 25(6): 623-33, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24746838

RESUMO

Omega-3 fatty acids (FAs) are essential nutritional components that must be obtained from foods. Increasing evidence validate that omega-3 FAs are beneficial for bone health, and several mechanisms have been suggested to mediate their effects on bone, including alterations in calcium absorption and urinary calcium loss, prostaglandin synthesis, lipid oxidation, osteoblast formation and inhibition of osteoclastogenesis. However, to date, there is scant information regarding the effect of omega-3 FAs on the developing skeleton during the rapid growth phase. In this study we aim to evaluate the effect of exposure to high levels of omega-3 FAs on bone development and quality during prenatal and early postnatal period. For this purpose, we used the fat-1 transgenic mice that have the ability to convert omega-6 to omega-3 fatty acids and the ATDC5 chondrogenic cell line as models. We show that exposure to high concentrations of omega-3 FAs at a young age accelerates bone growth through alterations of the growth plate, associated with increased chondrocyte proliferation and differentiation. We further propose that those effects are mediated by the receptors G-protein coupled receptor 120 (GPR120) and hepatic nuclear factor 4α, which are expressed by chondrocytes in culture. Additionally, using a combined study on the structural and mechanical bone parameters, we show that high omega-3 levels contribute to superior trabecular and cortical structure, as well as to stiffer bones and improved bone quality. Most interestingly, the fat-1 model allowed us to demonstrate the role of maternal high omega-3 concentration on bone growth during the gestation and postnatal period.


Assuntos
Desenvolvimento Ósseo , Doenças do Desenvolvimento Ósseo/prevenção & controle , Osso e Ossos/patologia , Ácidos Graxos Ômega-3/biossíntese , Osteogênese , Animais , Densidade Óssea , Doenças do Desenvolvimento Ósseo/enzimologia , Doenças do Desenvolvimento Ósseo/metabolismo , Doenças do Desenvolvimento Ósseo/patologia , Osso e Ossos/citologia , Osso e Ossos/metabolismo , Proteínas de Caenorhabditis elegans/genética , Proteínas de Caenorhabditis elegans/metabolismo , Linhagem Celular , Proliferação de Células , Condrócitos/citologia , Condrócitos/metabolismo , Condrócitos/patologia , Ácidos Graxos Dessaturases/genética , Ácidos Graxos Dessaturases/metabolismo , Ácidos Graxos Ômega-3/uso terapêutico , Feminino , Fator 4 Nuclear de Hepatócito/agonistas , Fator 4 Nuclear de Hepatócito/metabolismo , Heterozigoto , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Receptores Acoplados a Proteínas G/agonistas , Receptores Acoplados a Proteínas G/metabolismo , Caracteres Sexuais , Organismos Livres de Patógenos Específicos
7.
Am J Hum Genet ; 90(2): 282-9, 2012 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-22265014

RESUMO

Genitopatellar syndrome (GPS) is a skeletal dysplasia with cerebral and genital anomalies for which the molecular basis has not yet been determined. By exome sequencing, we found de novo heterozygous truncating mutations in KAT6B (lysine acetyltransferase 6B, formerly known as MYST4 and MORF) in three subjects; then by Sanger sequencing of KAT6B, we found similar mutations in three additional subjects. The mutant transcripts do not undergo nonsense-mediated decay in cells from subjects with GPS. In addition, human pathological analyses and mouse expression studies point to systemic roles of KAT6B in controlling organismal growth and development. Myst4 (the mouse orthologous gene) is expressed in mouse tissues corresponding to those affected by GPS. Phenotypic differences and similarities between GPS, the Say-Barber-Biesecker variant of Ohdo syndrome (caused by different mutations of KAT6B), and Rubinstein-Taybi syndrome (caused by mutations in other histone acetyltransferases) are discussed. Together, the data support an epigenetic dysregulation of the limb, brain, and genital developmental programs.


Assuntos
Histona Acetiltransferases/genética , Anormalidades Musculoesqueléticas/genética , Mutação , Anormalidades Urogenitais/genética , Anormalidades Múltiplas/enzimologia , Anormalidades Múltiplas/genética , Animais , Blefarofimose/enzimologia , Blefarofimose/genética , Blefaroptose/enzimologia , Blefaroptose/genética , Doenças do Desenvolvimento Ósseo/enzimologia , Doenças do Desenvolvimento Ósseo/genética , Cerebelo/anormalidades , Epigenômica/métodos , Exoma , Feminino , Cardiopatias Congênitas/enzimologia , Cardiopatias Congênitas/genética , Heterozigoto , Humanos , Deficiência Intelectual/enzimologia , Deficiência Intelectual/genética , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Anormalidades Musculoesqueléticas/enzimologia , Fenótipo , Síndrome de Rubinstein-Taybi/enzimologia , Síndrome de Rubinstein-Taybi/genética , Análise de Sequência de DNA/métodos , Anormalidades Urogenitais/enzimologia
8.
Am J Hum Genet ; 88(5): 608-15, 2011 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-21549340

RESUMO

We used whole-exome sequencing to study three individuals with a distinct condition characterized by short stature, chondrodysplasia with brachydactyly, congenital joint dislocations, cleft palate, and facial dysmorphism. Affected individuals carried homozygous missense mutations in IMPAD1, the gene coding for gPAPP, a Golgi-resident nucleotide phosphatase that hydrolyzes phosphoadenosine phosphate (PAP), the byproduct of sulfotransferase reactions, to AMP. The mutations affected residues in or adjacent to the phosphatase active site and are predicted to impair enzyme activity. A fourth unrelated patient was subsequently found to be homozygous for a premature termination codon in IMPAD1. Impad1 inactivation in mice has previously been shown to produce chondrodysplasia with abnormal joint formation and impaired proteoglycan sulfation. The human chondrodysplasia associated with gPAPP deficiency joins a growing number of skeletoarticular conditions associated with defective synthesis of sulfated proteoglycans, highlighting the importance of proteoglycans in the development of skeletal elements and joints.


Assuntos
Doenças do Desenvolvimento Ósseo/patologia , Artropatias/patologia , Mutação , Monoéster Fosfórico Hidrolases/genética , Sequência de Aminoácidos , Doenças do Desenvolvimento Ósseo/enzimologia , Feminino , Complexo de Golgi/enzimologia , Homozigoto , Humanos , Lactente , Recém-Nascido , Artropatias/enzimologia , Deformidades Congênitas dos Membros/patologia , Masculino , Dados de Sequência Molecular , Nucleotídeos/metabolismo , Fenótipo , Estrutura Quaternária de Proteína , Proteoglicanas/metabolismo , Sulfotransferases/metabolismo , Adulto Jovem
9.
Nat Genet ; 43(2): 127-31, 2011 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-21217755

RESUMO

We studied ten individuals from eight families showing features consistent with the immuno-osseous dysplasia spondyloenchondrodysplasia. Of particular note was the diverse spectrum of autoimmune phenotypes observed in these individuals (cases), including systemic lupus erythematosus, Sjögren's syndrome, hemolytic anemia, thrombocytopenia, hypothyroidism, inflammatory myositis, Raynaud's disease and vitiligo. Haplotype data indicated the disease gene to be on chromosome 19p13, and linkage analysis yielded a combined multipoint log(10) odds (LOD) score of 3.6. Sequencing of ACP5, encoding tartrate-resistant acid phosphatase, identified biallelic mutations in each of the cases studied, and in vivo testing confirmed a loss of expressed protein. All eight cases assayed showed elevated serum interferon alpha activity, and gene expression profiling in whole blood defined a type I interferon signature. Our findings reveal a previously unrecognized link between tartrate-resistant acid phosphatase activity and interferon metabolism and highlight the importance of type I interferon in the genesis of autoimmunity.


Assuntos
Fosfatase Ácida/deficiência , Fosfatase Ácida/genética , Doenças do Desenvolvimento Ósseo/genética , Doenças do Desenvolvimento Ósseo/patologia , Regulação da Expressão Gênica , Interferon Tipo I/metabolismo , Isoenzimas/deficiência , Isoenzimas/genética , Animais , Autoimunidade , Doenças do Desenvolvimento Ósseo/enzimologia , Bovinos , Cromossomos Humanos Par 19 , Feminino , Humanos , Inflamação , Lúpus Eritematoso Sistêmico/metabolismo , Masculino , Modelos Moleculares , Mutação , Mutação de Sentido Incorreto , Fenótipo , Esclerose/patologia , Fosfatase Ácida Resistente a Tartarato
11.
Clin Genet ; 74(4): 374-83, 2008 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-18554282

RESUMO

Mandibuloacral dysplasia type A (MADA; OMIM 248370), a rare disorder caused by mutation in the LMNA gene, is characterized by post-natal growth retardation, craniofacial and skeletal anomalies (mandibular and clavicular hypoplasia, acroosteolysis, delayed closure of cranial sutures, low bone mass and joint contractures), cutaneous changes and partial lipodystrophy. Little is known about the molecular mechanisms by which LMNA mutations produce bone alterations. An altered bone extracellular matrix (ECM) remodelling could play a pivotal role in this disorder and influence part of the typical bone phenotype observed in patients. Therefore, we have focused our investigation on matrix metalloproteinases (MMPs), which are degradative enzymes involved in ECM degradation and ECM remodelling, thus likely contributing to the altered bone mineral density and bone metabolism values seen in five MADA patients. We evaluated the serum levels of several MMPs involved in bone development, remodelling and homeostasis, such as MMP-9, -2, -3, -8 and -13, and found that only the 82 kDa active enzyme forms of MMP-9 are significantly higher in MADA sera compared with healthy controls (n = 16). The serum level of MMP-3 was instead lower in all patients. No significant differences were observed between controls and MADA patients for the serum levels of MMP-2, -8 and -13 and of tissue inhibitor of metalloproteinase 2, a natural inhibitor of MMP-9. Similarly, normal serum levels of tumour necrosis factor alpha (TNF-alpha), interleukin (IL)-6 and IL-1beta were detected. These data suggest a possible involvement of MMP-9 in MADA disease, underlying the potential use in diagnosis and therapy.


Assuntos
Senilidade Prematura/enzimologia , Doenças do Desenvolvimento Ósseo/enzimologia , Anormalidades Craniofaciais/enzimologia , Metaloproteinase 9 da Matriz/metabolismo , Adolescente , Adulto , Senilidade Prematura/genética , Doenças do Desenvolvimento Ósseo/genética , Criança , Pré-Escolar , Anormalidades Craniofaciais/genética , Feminino , Humanos , Masculino , Metaloproteinase 9 da Matriz/sangue , Metaloproteinase 9 da Matriz/genética , Síndrome
12.
Am J Hum Genet ; 82(6): 1368-74, 2008 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-18513679

RESUMO

Deficiency of carbohydrate sulfotransferase 3 (CHST3; also known as chondroitin-6-sulfotransferase) has been reported in a single kindred so far and in association with a phenotype of severe chondrodysplasia with progressive spinal involvement. We report eight CHST3 mutations in six unrelated individuals who presented at birth with congenital joint dislocations. These patients had been given a diagnosis of either Larsen syndrome (three individuals) or humero-spinal dysostosis (three individuals), and their clinical features included congenital dislocation of the knees, elbow joint dysplasia with subluxation and limited extension, hip dysplasia or dislocation, clubfoot, short stature, and kyphoscoliosis developing in late childhood. Analysis of chondroitin sulfate proteoglycans in dermal fibroblasts showed markedly decreased 6-O-sulfation but enhanced 4-O-sulfation, confirming functional impairment of CHST3 and distinguishing them from diastrophic dysplasia sulphate transporter (DTDST)-deficient cells. These observations provide a molecular basis for recessive Larsen syndrome and indicate that recessive Larsen syndrome, humero-spinal dysostosis, and spondyloepiphyseal dysplasia Omani type form a phenotypic spectrum.


Assuntos
Doenças do Desenvolvimento Ósseo/enzimologia , Doenças do Desenvolvimento Ósseo/genética , Disostoses/enzimologia , Disostoses/genética , Luxações Articulares/congênito , Luxações Articulares/genética , Mutação , Sulfotransferases/deficiência , Sulfotransferases/genética , Adolescente , Adulto , Criança , Pré-Escolar , Proteoglicanas de Sulfatos de Condroitina/química , Proteoglicanas de Sulfatos de Condroitina/metabolismo , Feminino , Genes Recessivos , Humanos , Úmero/anormalidades , Recém-Nascido , Luxações Articulares/enzimologia , Masculino , Osteocondrodisplasias/enzimologia , Osteocondrodisplasias/genética , Fenótipo , Coluna Vertebral/anormalidades , Síndrome , Carboidrato Sulfotransferases
13.
Curr Opin Pediatr ; 18(4): 435-41, 2006 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-16915000

RESUMO

PURPOSE OF REVIEW: P450 oxidoreductase deficiency--a newly described form of congenital adrenal hyperplasia--typically presents a steroid profile suggesting combined deficiencies of steroid 21-hydroxylase and 17alpha-hydroxylase/17,20-lyase activities. These and other enzymes require electron donation from P450 oxidoreductase. The clinical spectrum of P450 oxidoreductase deficiency ranges from severely affected children with ambiguous genitalia, adrenal insufficiency and the Antley-Bixler skeletal malformation syndrome to mildly affected individuals with polycystic ovary syndrome. We review current knowledge of P450 oxidoreductase deficiency and its broader implications. RECENT FINDINGS: Since the first report in 2004, at least 21 P450 oxidoreductase mutations have been reported in over 40 patients. The often subtle manifestations of P450 oxidoreductase deficiency suggest it may be relatively common. P450 oxidoreductase deficiency, with or without Antley-Bixler syndrome, is autosomal recessive, whereas Antley-Bixler syndrome without disordered steroidogenesis is caused by autosomal dominant fibroblast growth factor receptor 2 mutations. In-vitro assays of P450 oxidoreductase missense mutations based on P450 oxidoreductase-supported P450c17 activities provide excellent genotype/phenotype correlations. The causal connection between P450 oxidoreductase deficiency and disordered bone formation remains unclear. SUMMARY: P450 oxidoreductase mutations cause combined partial deficiency of 17alpha-hydroxylase and 21-hydroxylase. Individuals with an Antley-Bixler syndrome-like phenotype presenting with sexual ambiguity or other abnormalities in steroidogenesis should be analyzed for P450 oxidoreductase deficiency.


Assuntos
Hiperplasia Suprarrenal Congênita/enzimologia , Doenças do Desenvolvimento Ósseo/enzimologia , Doenças Ósseas Endócrinas/enzimologia , Doenças Ósseas Metabólicas/enzimologia , Sistema Enzimático do Citocromo P-450/metabolismo , Oxirredutases/deficiência , Hiperplasia Suprarrenal Congênita/complicações , Hiperplasia Suprarrenal Congênita/genética , Doenças do Desenvolvimento Ósseo/etiologia , Doenças do Desenvolvimento Ósseo/genética , Doenças Ósseas Endócrinas/etiologia , Doenças Ósseas Endócrinas/genética , Doenças Ósseas Metabólicas/etiologia , Doenças Ósseas Metabólicas/genética , Genótipo , Humanos , Fenótipo
14.
Biochim Biophys Acta ; 1761(7): 677-85, 2006 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-16784888

RESUMO

3Beta-hydroxysterol Delta(14)-reductase operates during the conversion of lanosterol to cholesterol in mammalian cells. Besides the endoplasmic reticulum 3beta-hydroxysterol Delta(14)-reductase (C14SR) encoded by TM7SF2 gene, the lamin B receptor (LBR) of the inner nuclear membrane possesses 3beta-hydroxysterol Delta(14)-reductase activity, based on its ability to complement C14SR-defective yeast strains. LBR was indicated as the primary 3beta-hydroxysterol Delta(14)-reductase in human cholesterol biosynthesis, since mutations in LBR gene were found in Greenberg skeletal dysplasia, characterized by accumulation of Delta(14)-unsaturated sterols. This study addresses the issue of C14SR and LBR role in cholesterol biosynthesis. Both human C14SR and LBR expressed in COS-1 cells exhibit 3beta-hydroxysterol Delta(14)-reductase activity in vitro. TM7SF2 mRNA and C14SR protein expression in HepG2 cells grown in delipidated serum (LPDS) plus lovastatin (sterol starvation) were 4- and 8-fold higher, respectively, than in LPDS plus 25-hydroxycholesterol (sterol feeding), resulting in 4-fold higher 3beta-hydroxysterol Delta(14)-reductase activity. No variations in LBR mRNA and protein levels were detected in the same conditions. The induction of TM7SF2 gene expression is turned-on by promoter activation in response to low cell sterol levels and is mediated by SREBP-2. The results suggest a primary role of C14SR in human cholesterol biosynthesis, whereas LBR role in the pathway remains unclear.


Assuntos
Colesterol/genética , Regulação da Expressão Gênica , Proteínas de Membrana/genética , Oxirredutases/metabolismo , Doenças do Desenvolvimento Ósseo/enzimologia , Doenças do Desenvolvimento Ósseo/genética , Colesterol/biossíntese , Humanos , Hidroxicolesteróis/farmacologia , Lovastatina/farmacologia , Proteínas de Membrana/metabolismo , Mutação , Oxirredutases/genética , Oxirredutases atuantes sobre Doadores de Grupo CH-CH , Receptores Citoplasmáticos e Nucleares/genética , Receptores Citoplasmáticos e Nucleares/metabolismo , Proteína de Ligação a Elemento Regulador de Esterol 2/metabolismo , Receptor de Lamina B
17.
Br J Nutr ; 71(6): 919-32, 1994 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-8031739

RESUMO

In the present study the effect of pyridoxine deficiency on the ultrastructure and morphology of bone and its metabolism was examined in the rapidly growing chick. Pyridoxine-deficient animals had tibias of reduced dry weight and cortical thickness. Histomorphometry demonstrated a disproportionately high eroded surface, lower amount of osteoid tissue and reduced mineralized trabecular width. Anterior-posterior radiographs of the tibiotarsometatarsal joint showed reduced secondary ossification centres and coarse trabeculation. Decalcified metaphyseal cartilage showed irregular trabeculas and a markedly reduced amount of Fast-green counterstain matrix suggesting that there is less collagen present and in turn less availability for matrix to be laid down for later calcification. Plasma activity of the bone alkaline phosphatase isoenzyme (EC 3.1.3.1) was decreased. Plasma Ca and PO4 levels did not vary. The present bone study referring to a pseudo-lathyritic state in which collagen maturation is not completely achieved supports the hypothesis that pyridoxine is an essential nutrient for the connective tissue matrix.


Assuntos
Doenças do Desenvolvimento Ósseo/veterinária , Galinhas , Artropatias/veterinária , Doenças das Aves Domésticas/etiologia , Deficiência de Vitamina B 6/veterinária , Fosfatase Alcalina/sangue , Animais , Artrografia , Doenças do Desenvolvimento Ósseo/diagnóstico por imagem , Doenças do Desenvolvimento Ósseo/enzimologia , Doenças do Desenvolvimento Ósseo/etiologia , Doenças do Desenvolvimento Ósseo/patologia , Cartilagem/patologia , Dieta , Isoenzimas , Artropatias/diagnóstico por imagem , Artropatias/etiologia , Artropatias/patologia , Masculino , Doenças das Aves Domésticas/diagnóstico por imagem , Doenças das Aves Domésticas/patologia , Tíbia/diagnóstico por imagem , Tíbia/patologia , Deficiência de Vitamina B 6/complicações
18.
Arch Dermatol ; 128(9): 1213-22, 1992 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-1519936

RESUMO

BACKGROUND AND DESIGN: Peroxisomal deficiency has been described in a number of syndromes characterized by chondrodysplasia punctata, including the Conradi-Hünermann (C-H) syndrome. Because of overlapping clinical features of X-chromosome inheritance, ichthyosis, and limb-reduction defects in C-H and CHILD (congenital hemidysplasia with ichthyosiform erythroderma and limb defects) syndromes, we examined peroxisomal content using diaminobenzidine cytochemistry and peroxisomal functions in fibroblasts from involved vs uninvolved skin of CHILD syndrome. RESULTS: Fibroblasts from involved skin of a patient with CHILD syndrome accumulated cytoplasmic lipid, visualized with the fluorescent probe, nile-red. Ultrastructurally, fibroblasts of involved skin of CHILD syndrome accumulated lamellated membrane and vacuolar structures. By diaminobenzidine ultracytochemistry, fewer peroxisomes were present. Moreover, the activities of two peroxisomal enzymes, catalase and dihydroxyacetone phosphate acyltransferase, were decreased (approximately 30% of normal). However, peroxisomal oxidation of very-long-chain and branched-chain fatty acids was preserved. Moreover, plasma very-long-chain fatty acids, plasma phytanic acid, and erythrocyte plasmalogen content were normal. CONCLUSIONS: The CHILD, C-H, and rhizomelic chondrodysplasia punctata syndromes are all characterized by ichthyosis, chondrodysplasia punctata, and limb defects, as well as peroxisomal deficiency. Thus, these syndromes may be related pathogenically. Because peroxisomes are involved in prostaglandin metabolism, peroxisomal deficiency may directly contribute to the previously reported alterations in prostaglandin metabolism in fibroblasts of involved skin of fibroblasts.


Assuntos
Aciltransferases/metabolismo , Doenças do Desenvolvimento Ósseo/enzimologia , Catalase/metabolismo , Condrodisplasia Punctata/enzimologia , Eritrodermia Ictiosiforme Congênita/enzimologia , Microcorpos/enzimologia , Dermatopatias/enzimologia , Doenças do Desenvolvimento Ósseo/complicações , Doenças do Desenvolvimento Ósseo/patologia , Células Cultivadas , Condrodisplasia Punctata/complicações , Condrodisplasia Punctata/patologia , Fibroblastos/enzimologia , Fibroblastos/ultraestrutura , Humanos , Eritrodermia Ictiosiforme Congênita/complicações , Eritrodermia Ictiosiforme Congênita/patologia , Microcorpos/ultraestrutura , Microscopia Eletrônica , Microscopia de Fluorescência , Dermatopatias/etiologia , Dermatopatias/patologia , Síndrome
19.
Biochem Int ; 24(1): 131-5, 1991 May.
Artigo em Inglês | MEDLINE | ID: mdl-1768253

RESUMO

Highly sensitive assay method of L-gulono-gamma-lactone oxidase (GLO) was constructed. In this method, L-ascorbic acid formed in the enzymatic reaction was converted to its bis(dinitrophenyl)hydrazone derivative, and the amount of the latter was determined by high-performance liquid chromatography. Twenty picomoles of ascorbic acid was detected, which makes this method 25 times more sensitive than the previously used dipyridyl one. By the present method, a minute activity of GLO in liver microsomes prepared from rats of the Osteogenic Disorder Shionogi strain (ODS-od/od) could be measured.


Assuntos
Desidrogenase do Álcool de Açúcar/análise , Animais , Ácido Ascórbico , Doenças do Desenvolvimento Ósseo/enzimologia , Doenças do Desenvolvimento Ósseo/genética , Cinética , L-Gulonolactona Oxidase , Microquímica , Microssomos Hepáticos/enzimologia , Ratos , Ratos Mutantes , Desidrogenase do Álcool de Açúcar/metabolismo
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