Integrated modeling of urban mobility, flood inundation, and sewer hydrodynamics processes to support resilience assessment of urban drainage systems.

With the increasing frequency of extreme weather events and a deepening understanding of disasters, resilience has received widespread attention in urban drainage systems. The studies on the resilience assessment of urban drainage systems are mostly indirect assessments that did not simulate human behavior affected by rainfall or semi-quantitative assessments that did not build simulation models, but few research characterizes the processes between people and infrastructure to assess resilience directly. Our study developed a dynamic model that integrates urban mobility, flood inundation, and sewer hydrodynamics processes. The model can simulate the impact of rainfall on people's mobility behavior and the full process including runoff generation, runoff entering pipes, node overflow, flood migration, urban mobility, and residential water usage. Then, we assessed the resilience of the urban drainage system under rainfall events from the perspectives of property loss and urban mobility. The study found that the average percentage increase in commuting time under different return periods of rainfall ranged from 6.4 to 203.9%. Calculating the annual expectation of property loss and traffic obstruction, the study found that the annual expectation loss in urban mobility is 9.1% of the annual expectation of property loss if the rainfall is near the morning commuting peak.

MODCEL-MHUS: a comprehensive multilayer hydrodynamic unified simulation for stormwater, sanitary sewer systems, and urban surface.

Numerous countries and regions have embraced implementing a separate sewer system, segregating sanitary and storm sewers into distinct systems. However, the functionality of these systems often needs to improve due to irregular interconnections, resulting in a mixed and malfunctioning system. Sewage collection is crucial for residential sanitation, but untreated collection significantly contributes to environmental degradation. Analyzing the simultaneous operation of both systems becomes vital for effective management. Using mathematical tools for precise and unified diagnosis and prognosis becomes imperative. However, municipal professionals and companies need more tools specifically designed to evaluate these systems in a unified way, mapping all the hydraulic connections observed in practice. This study proposes a unified simulation method for stormwater and sanitary sewer urban systems, addressing real-world scenarios and potential interferences. The primary goal is to develop a simulation method for both systems, considering system interconnections and urban layouts, involving hydrodynamic and water quality simulations. The practical application of this method, the Multilayer Hydrodynamic Simulation Method (MODCEL-MHUS), successfully identifies issues in urban water networks and suggests solutions, making it a valuable tool for urban water management and environmental engineering professionals.

Green infrastructure layout based on a dynamic operation feature of drainage systems.

Increasingly frequent urban floods strain the traditional grey infrastructure, overwhelming the capacity of drainage networks and causing challenges in managing stormwater. The heavy precipitation leads to flooding and damage to drainage systems. Consequently, efficient mitigation strategies for flooding have been researched deeply. Green infrastructure (GI) has proved to be effective in responding the increasing risk of flood and alleviate pressure on drainage systems. However, as the primary infrastructure of stormwater management, there is still a lack of attention to the dynamic operation feature of urban sewer systems during precipitation events. To fill this gap, we proposed a novel approach that integrates hydraulic characteristics and the topological structure of a sewer network system. This approach aims to identify influential nodes, which contribute to the connectivity of the sewer network amidst dynamic changes in inflow during precipitation events. Furthermore, we adopted rain barrels to serve as exemplars of GI, and 14 GI layout schemes are produced based on the different ranks of influential nodes. Implementing GI measures on both poorly performing and well-performing nodes can yield distinct benefits in mitigating node flooding. This approach provides a new perspective for stormwater management, establishing effective synergy between GI and the drainage system.

Urban flood modeling with a novel coupling method of surface and sewer hydrodynamic processes.

Drainage modeling that accurately captures urban storm inundation serves as the foundation for flood warning and drainage scheduling. In this paper, we proposed a novel coupling ideology that, by integrating 2D-1D and 1D-2D unidirectional processes, overcomes the drawback of the conventional unidirectional coupling approach that fails to properly represent the rainfall surface catchment dynamics, and provides more coherent hydrological implications compared to the bidirectional coupling concept. This paper first referred to a laboratory experimental case from the literature, applied and analyzed the coupling scheme proposed in this paper and the bidirectional coupling scheme that has been widely studied in recent years, compared the two coupling solutions in terms of the resulting accuracy and applicability, and discussed their respective strengths and weaknesses to validate the reliability of the proposed method. The verified proposed coupling scheme was then applied to the modeling of a real drainage system in a region of Nanjing, China, and the results proved that the coupling mechanism proposed in this study is of practical application value.

Tunable linear feedback control of urban drainage systems using models defined purely from data.

Real-time and model-predictive control promises to make urban drainage systems (UDS) adaptive, coordinated, and dynamically optimal. Though early implementations are promising, existing control algorithms have drawbacks in computational expense, trust, system-level coordination, and labor cost. Linear feedback control has distinct advantages in computational expense, interpretation, and coordination. However, current methods for building linear feedback controllers require calibrated software models. Here we present an automated method for generating tunable linear feedback controllers that require only system response data. The controller design consists of three main steps: (1) estimating the network connectivity using tools for causal inference, (2) identifying a linear, time-invariant (LTI) dynamical system which approximates the network, and (3) designing and tuning a feedback controller based on the LTI urban drainage system approximation. The flooding safety, erosion prevention, and water treatment performance of the method are evaluated across 190 design storms on a separated sewer model. Strong results suggest that the system knowledge required for generating effective, safe, and tunable controllers for UDS is surprisingly basic. This method allows near-turnkey synthesis of controllers solely from sensor data or reduction of process-based models.

Real-time control of urban drainage systems using neuro-evolution.

With climate change and urbanization, existing urban drainage systems are being stressed beyond their design capacity in many parts of the world. Real-time control (RTC) can improve the performance of these systems and reduce the need for system upgrades. However, developing optimal control policies for RTC is a challenging research area due to computational demands, high uncertainties and system dynamics. This study presents a new RTC method using neuro-evolution for controlling combined sewer overflow (CSO) in urban drainage systems. Neuro-evolution is an approach to neural network research by evolutionary algorithms. Neuro-evolution realizes RTC by training the control policy in advance, thus avoiding the online optimization process in the application period. The simulation results of the benchmark Astlingen network indicate that the trained control policy outperforms the equal filling degree strategy in terms of CSO volume reduction and robustness in the face of tank level uncertainty. The performance analysis of the typical CSO events shows that the control policy mainly makes positive contributions during 'small' CSO events rather than 'large' ones. In particular, the effectiveness of the control policy in 'small' CSO events is more prominent in the initial phase of the events compared with the final phase. This work stands to support a foundation for future studies in the control of urban water systems based on neuro-evolution.

A dynamic one-dimensional model for simulating unsteady air-water stratified flow in sewer pipes.

Ventilation is paramount in sanitary and stormwater sewer systems to mitigate odor problems and avert pressure surges. Existing numerical models have constraints in practical applications in actual sewer systems due to insufficient airflow modeling or suitability only for steady-state conditions. This research endeavors to formulate a mathematical model capable of accurately simulating various operational conditions of sewer systems under the natural ventilation condition. The dynamic water flow is modeled using a shock-capturing MacCormack scheme. The dynamic airflow model amalgamates energy and momentum equations, circumventing laborious pressure iteration computations. This model utilizes friction coefficients at interfaces to enhance the description of the momentum exchange in the airflow and provide a logical explanation for air pressure. A systematic analysis indicates that this model can be easily adapted to include complex boundary conditions, facilitating its use for modeling airflow in real sewer networks. Furthermore, this research uncovers a direct correlation between the air-to-water flow rate ratio and the filling ratio under natural ventilation conditions, and an empirical formula encapsulating this relationship is derived. This finding offers insights for practical engineering applications.

Elucidating the impact of sanitary waste on the formation of fat, oil and grease deposits in sewer systems.

The accumulation of fat, oil and grease (FOG) deposits in sanitary sewer systems is a significant cause of sewer overflows, mainly due to their tendency to adhere to pipe walls. The aim of this study is to (i) develop laboratory-prepared FOG deposits using a mixture of iron (Fe) and aluminium (Al) metal ions, fatty acids, saccharides and cooked oils, in addition to various sanitary waste materials such as paper towels, wipes and pads and (ii) examine the characteristics of these FOG deposits. The goals of this study were to (i) gain a deeper understanding of the impact of sanitary waste on the formation of FOG deposits and (ii) discuss the detailed physiochemical properties of these FOG deposits. The findings revealed that FOG deposits can vary in nature, appearing as either a smooth, paste-like substance or a coarse, semi-solid material, depending on the types of waste present in the sewer. Analysis of the fatty acid profile indicated that the FOG deposits with wipes have the highest viscosity (3.2 × 104 Pa s) and larger composition of smaller chain saturated fatty acids (caprylic acid 0.64%, undecanoic acid 5.61%, lauric acid 4.65%, myristic acid 3.21% and palmitic 8.38%). In contrast, FOG deposits with Fe and Al metal impurities have higher heat resistance and thermal stability (melting point of 125 °C) and have larger composition of long chain fatty acids. Furthermore, FTIR analysis confirmed that these FOG deposits are composed of metallic salts of fatty acids, aligning with samples from sewer lines. Our results suggest that FOG deposit formation involves the aggregation of excess calcium, which compresses free fatty acid micelles, and a saponification reaction between the calcium aggregates and free fatty acids. This research illuminates the complex processes behind FOG deposit formation and their varied characteristics, providing valuable insights into potential strategies for preventing FOG-related sewer blockages.

Automated screening of control potential with spatially explicit results to support dialogue about sewer overflow reduction and beyond.

For real-time control to become a standard measure for upgrading urban drainage systems, control potential screenings need to be easily integrated into the early planning processes that already take place. However, current screening methods are either not aligned with the present planning process, unrelatable for water managers or too time-consuming. We therefore developed an automated screening methodology through a co-design process with six Danish utilities. The process started out from a literature review, included interviews and workshops, and resulted in the control potential screening tool COPOTO. In the co-design process, utilities generally responded that indicators based solely on an assessment of static system attributes are insufficient. Thus, COPOTO instead post-processes the results of urban drainage simulation models that are commonly available. The decision context considered in initial planning phases was found to include environmental, economic, social and technical objectives that were highly case-dependent. When presenting CSO reduction potentials, the utilities therefore generally preferred interactive, spatially explicit visualisations that link the CSO reduction at a particular location to the storages and actuators that need to be activated. This enables water managers to discuss, for example, operational constraints of a considered control location. COPOTO provides such assessments with very limited manual and computational effort and thus facilitates the integration of real-time control into standard planning workflows of utilities.

Classification and description of the drainage state of manholes in urban drainage systems.

Manholes are important structures in urban storm drainage systems connecting roads and underground drainage networks, and they are also an important part of the research on improving urban resistance to storm flooding. Due to cost and space constraints, most of the existing experimental data on manholes come from scale model experiments obtained by scaling according to Froude's similarity criterion, and there is a lack of validation based on full-size experimental data. This also leads to inconsistencies in the form and parameter values of the manhole flow exchange equations derived from different experiments. To remedy this deficiency, a full-scale urban drainage engineering physics model was developed in this study with the aim of investigating the flow exchange of surface water as it flows through manholes into the sewer system. Experiments were conducted under steady flow conditions and compared with predictions from the existing models. The results show that the predictions of the existing model deviate significantly from the measured values when the flow is between free weir flow and submerged orifice flow. Therefore, we constructed a weighting equation based on weir and orifice flows and found that the weighting coefficients decayed exponentially during the transition from weir to orifice flow.

Management of the designed risk level of urban drainage system in the future: Evidence from haining city, China.

The design of urban drainage infrastructure is mainly based on historical conditions. Under global warming, more intense precipitation extremes will pose severe risk to current infrastructure. The evaluation of where and by how much design standards need to change, is urgently needed to help maintain well-functioning drainage systems. In this study, we used climate projections from the Coupled Model Intercomparison Project Phase 6 (CMIP6) and InfoWorks Integrated Catchment Modeling (ICM) to simulate urban flooding. According to the latest design standard of urban drainage infrastructure, we assess the risk of future urban flooding, and evaluate the effect and benefit of drainage infrastructure adaptation measures. The results showed that, under the shared socioeconomic pathway (SSP) 5-8.5 scenario, a 35% increase in extreme rainfall would be expected. Under a 1-in-30-year precipitation event, the maximum depth would increase by 5.59%, and the withdrawal time would rise by 2.94% in the future period, relative to the baseline level. After the enlargement of drainage infrastructure in local areas, 10% pipe enlargement has a better effect to reduce risk and higher benefits than 5% pipe enlargement. These findings provide valuable insights for policymakers in enhancing the drainage system and adapting to climate change.

Prediction of the roughness coefficient for drainage pipelines with sediments using GA-BPNN.

Accurate prediction of the roughness coefficient of sediment-containing drainage pipes can help engineers optimize urban drainage systems. In this paper, the variation of the roughness coefficient of circular drainage pipes containing different thicknesses of sediments under different flows and slopes was studied by experimental measurements. Back Propagation Neural Network (BPNN) and Genetic Algorithm-Back Propagation Neural Network (GA-BPNN) were used to predict the roughness coefficient. To explore the potential of artificial neural networks to predict the roughness coefficient, a formula based on drag segmentation was established to calculate the roughness coefficient. The results show that the variation trend of the roughness coefficient with flow, hydraulic radius, and Reynolds number is consistent. With the increase of the three parameters, the roughness coefficient decreases overall. Compared to the traditional empirical formula, the BPNN model and the GA-BPNN model increased the determination factors in the testing stage by 3.47 and 3.99%, respectively, and reduced the mean absolute errors by 41.18 and 47.06%, respectively. The study provides an intelligent method for accurate prediction of sediment-containing drainage pipes roughness coefficient.

Physical modelling of energy losses at surcharged three-way junction manholes in drainage system.

Motivated by the observation that vortex flow structure was evident in the energy loss at the surcharged junction manhole due to changes of hydraulic and geometrical parameters, a physical model was used to calculate energy loss coefficients and investigate the relationship between flow structure and energy loss at the surcharged three-way junction manhole. The effects of the flow discharge ratio, the connected angle between two inflow pipes, the manhole geometry, and the downstream water depth on the energy loss were analyzed based on the quantified energy loss coefficients and the identified flow structure. Moreover, two empirical formulae for head loss coefficients were validated by the experimental data. Results indicate that the effect of flow discharge ratio and connected angle are significant, while the effect of downstream water depth is not obvious. With the increase of the lateral inflow discharge, the flow velocity distribution and vortex structure are both enhanced. It is also found that a circular manhole can reduce local energy loss when compared to a square manhole. In addition, the tested empirical formulae can reproduce the trend of total head loss coefficient.

A biogeographic 16S rRNA survey of bacterial communities of ureolytic biomineralization from California public restrooms.

In this study, we examined the total bacterial community associated with ureolytic biomineralization from urine drainage systems. Biomineral samples were obtained from 11 California Department of Transportation public restrooms fitted with waterless, low-flow, or conventional urinals in 2019. Following high throughput 16S rRNA Illumina sequences processed using the DADA2 pipeline, the microbial diversity assessment of 169 biomineral and urine samples resulted in 3,869 reference sequences aggregated as 598 operational taxonomic units (OTUs). Using PERMANOVA testing, we found strong, significant differences between biomineral samples grouped by intrasystem sampling location and urinal type. Biomineral microbial community profiles and alpha diversities differed significantly when controlling for sampling season. Observational statistics revealed that biomineral samples obtained from waterless urinals contained the largest ureC/16S gene copy ratios and were the least diverse urinal type in terms of Shannon indices. Waterless urinal biomineral samples were largely dominated by the Bacilli class (86.1%) compared to low-flow (41.3%) and conventional samples (20.5%), and had the fewest genera that account for less than 2.5% relative abundance per OTU. Our findings are useful for future microbial ecology studies of urine source-separation technologies, as we have established a comparative basis using a large sample size and study area.

Análise das oportunidades e desafios no uso de Soluções baseadas na Natureza como medidas complementares ao "Programa Novo Rio Pinheiros" na cidade de São Paulo / Analysis of opportunities and challenges in the use of Nature-based Solutions as complementary measures to the "Novo Rio Pinheiros Program" in the city of São Paulo

As deficiências na infraestrutura sanitária e urbana, a ineficiente gestão dos resíduos sólidos urbanos, as ocupações das planícies aluviais e a impermeabilização do solo são alguns dos principais fatores que condicionam ao estado de poluição em cursos hídricos, bem como potencializam os riscos de transbordamentos de rios e córregos urbanos. A conservação de bacias hidrográficas tem se tornado essenciais frente ao desafio de assegurar a disponibilidade de água em padrões de qualidade. Implantado em 2019, o Programa Novo Rio Pinheiros, tem a meta de reduzir o esgoto lançado em seus afluentes e melhorar a qualidade das águas até 2022. Com o objetivo de contribuir no alcance das metas do Programa, analisou-se o potencial das Soluções baseadas na Natureza (SbN) como medidas complementares às infraestruturas convencionais de saneamento. Para tanto, a pesquisa foi dividida em três partes, a primeira analisou estudos de caso com SbN por meio de uma revisão sistemática de literatura. A segunda e terceira compreenderam estudos que identificaram as sub-bacias dos córregos do Jaguaré, Pirajuçara, Pau Arcado/Morumbi, Morro do S, Cordeiro, Água Espraiada, Zavuvus, Pedreira, Olaria, Poli, Sapateiro, e o dreno do Brooklin, em condições mais críticas de poluição das águas superficiais ou de alagamentos urbanos na bacia hidrográfica do rio Pinheiros. Os resultados identificaram que as SbN proporcionam oportunidades como: (1) solução decentralizada e de baixo custo para conter alagamento e poluição na água, fornecendo alternativas de tratamento próximo das fontes geradoras; (2) soluções eficientes para remover compostos de fósforo, nitrogênio, substâncias orgânicas e sedimentos; (3) recuperação de áreas degradadas e contaminadas; e (4) integração dos serviços ecossistêmicos e paisagísticos. O desafio no uso de SbN está na apropriação dos espaços urbanos densamente povoados, sendo que as regiões centrais com urbanização mais consolidada, que dispõe de mais áreas verdes como as praças e parques, as tipologias de SbN como biovaletas e os jardins pluviais são mais favoráveis e, nas regiões periféricas, com a predominância de ruas estreitas e ausência de áreas verdes, os parques lineares são mais favoráveis para melhorar a qualidade ambiental dos córregos e a ampliação de espaços de lazer. Concluiu-se que o uso de SbN tem o potencial de aprimorar as ações do eixo saneamento, contribuindo para alcançar as metas do Programa Novo Rio Pinheiros.

Deficiencies in sanitary and urban infrastructure, inefficient management of urban solid waste, occupation of floodplains and soil sealing are some of the main factors that condition the state of pollution in water courses, as well as potentiate the risks of river overflows. and urban streams. The conservation of watersheds has become essential in the face of the challenge of ensuring the availability of water with quality standards. Implemented in 2019, the Novo Rio Pinheiros Program has the goal of reducing the sewage discharged into its tributaries and improving water quality by 2022. In order to contribute to the achievement of the Program's goals, the potential of Nature-based Solution (NbS) as complementary measures to conventional sanitation infrastructures. Therefore, the research was divided into three parts, the first analyzed case studies with NbS through a systematic literature review. The second and third comprised studies that identified the sub-basins streams of Jaguaré, Pirajuçara, Pau Arcado/Morumbi, Morro do S, Cordeiro, Água Espraiada, Zavuvus, Pedreira, Olaria, Poli, Sapateiro, and the Brooklin drain, in more critical conditions of surface water pollution or urban flooding in the Pinheiros river basin. The results identified that the NbS provide opportunities such as: (1) a decentralized and low-cost solution to contain flooding and water pollution, providing treatment alternatives close to the generating sources; (2) efficient solutions to remove phosphorus compounds, nitrogen, organic substances and sediments; (3) recovery of degraded and contaminated areas; and (4) integration of ecosystem and landscape services. The challenge in using NbS is in the appropriation of densely populated urban spaces, with central regions with more consolidated urbanization, which have more green areas such as squares and parks, NbS typologies such as biovaletas and rain gardens are more favorable, although in peripheral regions, with the predominance of narrow streets and the absence of green areas, the linear parks are more favorable to improve the environmental quality of streams and the expansion of leisure spaces. It was concluded that the use of NbS has the potential to improve the actions of the sanitation axis, contributing to achieving the goals of the Novo Rio Pinheiros Program.

The Effect of Flooding and Drainage Duration on the Release of Trace Elements from Floodplain Soils.

Floodplains downstream of urban catchments are sinks for potentially toxic trace elements. An intensification of the hydrological cycle and changing land use will result in floodplains becoming inundated for longer durations in the future. We collected intact soil cores from a floodplain meadow downstream of an urban catchment and subjected them to an inundation/drainage cycle in the laboratory to investigate the effect of flood duration on trace element concentrations in the soil porewater. The porewater concentrations of Ni, Cr, and Zn increased, whereas Cu and Pb decreased with flood duration. All the Cr present in porewaters was identified as Cr(III). Copper concentrations increased after drainage but Pb mobility remained suppressed. Both pH and dissolved organic carbon (DOC) increased with flood duration but were lower in treatments that were drained for the longest duration (which were also the treatments flooded for the shortest duration). The porewater concentrations of Cr and Ni decreased after drainage to levels below those observed before inundation, mirroring the DOC concentrations. We concluded that the duration of floodplain inundation does have an influence on the environmental fate of trace elements but that flooding does not influence all trace elements in the same way. The implications of an intensification of the hydrological cycle over the coming decades are that floodplains may become a source of some trace elements to aquatic and terrestrial ecosystems. Environ Toxicol Chem 2020;39:2124-2135. © 2020 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Toilet drain water as a potential source of hospital room-to-room transmission of carbapenemase-producing Klebsiella pneumoniae.

BACKGROUND: Carbapenemase-producing Enterobacterales (CPE) have rapidly emerged in Europe, being responsible for nosocomial outbreaks. AIM: Following an outbreak in the burn unit of Ghent University Hospital, we investigated whether CPE can spread between toilets through drain water and therefrom be transmitted to patients. METHODS: In 2017, the burn centre of our hospital experienced an outbreak of OXA-48-producing Klebsiella pneumoniae that affected five patients staying in three different rooms. Environmental samples were collected from the sink, shower, shower stretcher, hand rail of the bed, nursing carts, toilets, and drain water to explore a common source. Whole-genome sequencing and phylogenetic analysis was performed on K. pneumoniae outbreak isolates and two random K. pneumoniae isolates. FINDINGS: OXA-48-producing K. pneumoniae was detected in toilet water in four out of six rooms and drain water between two rooms. The strain persisted in two out of six rooms after two months of daily disinfection with bleach. All outbreak isolates belonged to sequence type (ST) 15 and showed isogenicity (<15 allele differences). This suggests that the strain may have spread between rooms by drain water. Unexpectedly, one random isolate obtained from a patient who became colonized while residing at the geriatric ward clustered with the outbreak isolates, suggesting the outbreak to be larger than expected. Daily application of bleach tended to be superior to acetic acid to disinfect toilet water; however, disinfection did not completely prevent the presence of carbapenemase-producing K. pneumoniae in toilet water. CONCLUSION: Toilet drain water may be a potential source of hospital room-to-room transmission of carbapenemase-producing K. pneumoniae.

Initial allocation of flood drainage rights based on a PSR model and entropy-based matter-element theory in the Sunan Canal, China.

The pursuit of flood prevention safety and the mitigation of drainage contradiction against an unnecessary influx of floodwater require a modern and efficient model to optimize the management of the initial allocation of flood drainage rights. We attempted to formulate a framework for initial flood drainage rights allocation to promote the sustainable drainage of the Sunan Canal, China. The Pressure-State-Response (PSR) model was constructed using a literature review and interviews with experts and directors using 18 key indicators being determined from field surveys and library studies. We then assessed the flood status of Zhenjiang City, Changzhou City, Wuxi City and Suzhou City in the Sunan Canal zone using an entropy-based matter-element model. The flood drainage rights for a total of 400m3/s was allocated to the four cities in accordance with their flood status. Our research demonstrated that, overall, the four cities may gain the flood drainage rights of 106.67m3/s,120.40m3/s, 118.22m3/s and 54.71m3/s, respectively. Specifically, the calculation of the flood drainage for Wuxi was very close to the actual allocation in 2016, whereas there were differences in the other cities that should not be neglected.

Characteristics of sewer biofilms in aerobic rural small diameter gravity sewers.

Small diameter gravity sewers (SDGS) are extensively used to collect rural sewage as they are low in cost and quick to construct. However, the characteristics of biofilms in rural SDGS are still not clear. In this study, biofilms characteristics of aerobic rural SDGS were investigated using simulations in a lab under different flow conditions and slopes. Results indicated that the average thickness of aerobic rural SDGS biofilms was in the range of 350-650 µm, decreasing at locations with variable flow and high slopes. Protein was the most abundant substance in extracellular polymeric substance of SDGS biofilms. The most abundant bacteria, Proteobacteria, Actinobacteria, and Bacteroidetes, and functional bacteria showed different distributions when analyzed through Illumina HiSeq sequencing of 16S rRNA. The relative abundances of denitrifying bacteria, nitrite-oxidizing bacteria, and sulfate-reducing bacteria (SRB) were lower during variable flow than during stable flow. High slopes (15‰) decreased SRB presence, which could be used to mitigate H2S accumulation in aerobic SDGS. Overall, this study describes the characteristics of aerobic rural SDGS biofilms and provides valuable suggestions for the optimal design of SDGS based on these characteristics.

Production of Culex pipiens in Stormwater and Combined Sewer Catch Basins.

Man-made stormwater and sewage infrastructure, particularly roadside catch basins, provides widespread habitats for immature mosquitoes in urban and suburban environments. Historically, throughout much of the USA, stormwater, sewage, and industrial wastewater were conducted together through "combined" sewer systems, discharging a combination of stormwater and wastewater into streams. Within recent decades, many cities have replaced these combined sewers with "stormwater only" systems that separate stormwater from wastewater. The objective of this research was to evaluate the implications of this infrastructure conversion for production of Culex pipiens, a primary vector for West Nile virus. On a weekly basis over 14 wk, 20 catch basins (10 combined sewer and 10 stormwater only) were sampled for mosquito larvae and emerging adults using the dipping collection method and floating emergence traps. Abundance of larval Cx. pipiens was higher in combined sewer compared with stormwater-only catch basins, while to the contrary, abundance of adult Cx. pipiens was lower in combined sewer compared with stormwater-only catch basins. This study is the first to reveal that habitat attractiveness and quality for Cx. pipiens may vary between combined sewer and stormwater-only catch basins, and our results contribute to a growing body of research to inform vector management and urban planning efforts as municipalities consider the environmental and public health implications of conversion from combined sewage management to separation of stormwater and wastewater.