RESUMO
En la práctica diaria, cuando se realiza la determinación de creatinquinasa isoenzima MB (CK-MB) por el método inmunológico, es habitual obtener resultados incongruentes: valores de CK-MB cercanos o superiores al de creatinquinasa total (CK). En el presente trabajo se informa acerca de las interferencias más frecuentes encontradas con este método en nuestro Instituto
Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Creatina Quinase/sangue , Testes Imunológicos/métodos , Creatina Quinase , Creatina Quinase/sangue , Creatina Quinase/imunologia , Creatina Quinase/isolamento & purificação , Eletroforese em Acetato de Celulose/normas , Eletroforese em Acetato de Celulose/estatística & dados numéricosRESUMO
En la práctica diaria, cuando se realiza la determinación de creatinquinasa isoenzima MB (CK-MB) por el método inmunológico, es habitual obtener resultados incongruentes: valores de CK-MB cercanos o superiores al de creatinquinasa total (CK). En el presente trabajo se informa acerca de las interferencias más frecuentes encontradas con este método en nuestro Instituto (AU)
Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Creatina Quinase/sangue , Testes Imunológicos/métodos , Creatina Quinase/imunologia , Eletroforese em Acetato de Celulose/normas , Eletroforese em Acetato de Celulose/estatística & dados numéricos , Creatina Quinase/isolamento & purificação , Creatina Quinase/sangue , Creatina Quinase/diagnósticoRESUMO
Human isonucleotidases were separated by electrophoresis on a cellulose acetate membrane. Three 5'-nucleotidase forms, NTP1, NTP2, and NTP3, were resolved with this method and quantified by densitometry. The procedure was not only simple and rapid but also sufficiently precise (between-run CV < 20%) and sensitive (detected nucleotidase fractions of > 0.5 U/L). The effects of various treatments (heat, neuraminidase, glycosidases, proteases, lectins, and detergents) on the electrophoretic pattern of 5'-nucleotidase were studied. NTP1 (mean 12% of total 5'-nucleotidase, SD 5%), NTP2 (mean 30%, SD 8%), and NTP3 (mean 58%, SD 8%) were found in all normal persons studied. The increase in total 5'-nucleotidase in patients with hepatobiliary disease was mainly due to the NTP1 isoform.
Assuntos
5'-Nucleotidase/isolamento & purificação , Eletroforese em Acetato de Celulose , Isoenzimas/isolamento & purificação , 5'-Nucleotidase/análise , 5'-Nucleotidase/sangue , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Doenças dos Ductos Biliares/enzimologia , Eletroforese em Acetato de Celulose/estatística & dados numéricos , Endopeptidases/farmacologia , Feminino , Glicosídeo Hidrolases/farmacologia , Temperatura Alta , Humanos , Isoenzimas/análise , Isoenzimas/sangue , Hepatopatias/enzimologia , Masculino , Pessoa de Meia-Idade , Neuraminidase/farmacologia , Octoxinol/farmacologia , Sensibilidade e Especificidade , Distribuição Tecidual , Aglutininas do Germe de Trigo/farmacologiaRESUMO
We adapted the electrophoretic method of bone alkaline phosphatase (ALP) determination using neuraminidase from Vibrio cholerae to separate bone and liver ALP on cellulose acetate membrane. Treatment of separator plus serum (1:8, neuraminidase 111 U/l in final) for 10 min at room temperature (25 +/- 1 degree C) and subsequent electrophoresis made it possible to quantify bone ALP activity simply and rapidly. The precision of the data was at the level of CV of 1.6% (within-day) and 4.7% (day-to-day), with recovery rates of 97-103%. The normal range of bone ALP activity depended on age and sex. Seventy-eight diabetes mellitus (DM) patients, excluding those with renal failure, were divided into two groups of those with and without osteopenia with matching of age (+/- 3 years) and sex. Bone ALP (P < 0.001) and total ALP (P < 0.05) activities and urine calcium/creatinine ratio (P < 0.05) were significantly higher in DM with osteopenia than in DM without osteopenia. Therefore, bone formation and absorption may be accelerated in DM with osteopenia in comparison with DM without osteopenia.
