RESUMO
A paper ionophoretic method is described for the study of equilibria in mixed ligand (nitrilotriacetate-cysteine) complex system in solution. The proportion of ionic species of nitrilotriacetate (NTA) and cysteine were varied by changing the pH of background electrolyte. The stability constants of Cu(II)-NTA-cysteine and Co(II)-NTA-cysteine complexes were found to be 6.35+/-0.05 and 5.45+/-0.02 (logarithm stability constant values), respectively, at ionic strength 0.1 M and a temperature of 35 degrees C.
Assuntos
Cobalto/química , Cobre/química , Cisteína/química , Eletroforese em Papel/métodos , Ácido Nitrilotriacético/químicaRESUMO
Stability constants of binary Fe(III)-methylcysteine, Cr(III)-methylcysteine and mixed Fe(III)-methylcysteine-cysteine, Cr(III)-methylcysteine-cysteine complexes have been determined by paper electrophoresis at 0.1 M ionic strength and a temperature of 35 degrees C. The stability constants of Fe(III)-methylcysteine-cysteine and Cr(III)-methylcysteine-cysteine mixed complexes were found to be 6.00 +/- 0.07 and 5.05 +/- 0.15 (logarithm of stability constant values), respectively.
Assuntos
Quelantes/química , Ácido Cisteico/química , Cisteína/química , Eletroforese em Papel/métodos , Metais/química , Ácido Cisteico/análogos & derivadosRESUMO
El estudio de las alteraciones del metabolismo de los aminoacidos urinarios ha motivado el desarrollo de dos tecnicas simples, rapidas y economicas, para el uso en Quimica clinica. Se describe la celda electroforetica disenada, asi como todas las operaciones requeridas para lograr dicho objetivo (tratamiento de los soportes usados y de las muestras, buffers, condiciones de corrida, distintos metodos y reactivos de revelado, desintometria). Se comparan los resultados obtenidos para los soportes empleados, papel y acetato de celulosa gelatinizado, usando para ambos igual sistema de refrigeracion y los resultados obtenidos en comparacion con otra forma de enfriamiento del sistema, utilizada previamente. Tambien se discute la aplicacion de mayores voltajes y la importancia de la estandarizacion en la coloracion. Trabajando con acetato de celulosa gelatinizado se pueden valorar por densitometria las corridas, lo cual brinda una util herramienta a los laboratorios clinicos
Assuntos
Recém-Nascido , Humanos , Estudo Comparativo , Eletroforese em Acetato de Celulose/métodos , Eletroforese em Papel/métodos , Aminoácidos/urina , Aminoácidos/análise , Densitometria/métodos , Aminoacidúrias Renais/diagnósticoRESUMO
Hemoglobin O Arab was found in 25 members of four apparently unrelated negro families in the West Indies of Jamaica. In each family the propositus had Hb SO disease. Two cases had been mistakenly diagnosed as Hb SC disease. Two persons heterozygous for both Hb C and Hb O Arab were found in these families, and Hb O Arab áthalassemia in one other relative. The clinical course and symptomatology of Hb SO disease is incomparable to that in Hb SD (O2á2121 Glu-> GluNH2) disease and more severe than Hb S2C2 disease. In vitro mixtures of Hb O Arab and Hb S change from a liquid to a gel phase at total hemoglobin concentrations weaker than those required to gel pure Hb S, whereas mixtures of Hb S with Hb A or Hb C require a stronger total hemoglobin concentration before gelling will occur. Oxygen dissociation studies on red cells containing Hb SO show a lowered oxygen affinity comparable to that found in homozygous sickle-cell anemia and outside the range for subjects with sickle-cell Hb C disease.(AU)
Assuntos
Adulto , Pré-Escolar , Lactente , Relatos de Casos , Feminino , Humanos , Masculino , Adolescente , Técnicas In Vitro , Hemoglobina Falciforme/análise , Hemoglobina A/análise , Hemoglobina C/análise , Jamaica , Negro ou Afro-Americano , /genética , Hemoglobina Falciforme , Anemia Falciforme/sangue , Eletroforese em Gel de Ágar/métodos , Eletroforese em Papel/métodosRESUMO
It is widely claimed that people living in the tropics, particularly negroes, have higher gamma globulin levels than Europeans. No such difference was found in a series of healthy Jamaicans and of healthy Europeans recently arrived in Jamaica. The zinc sulphate turbidity test does not measure gamma globulins alone (AU)
Assuntos
Humanos , Adulto , Masculino , Feminino , Técnicas In Vitro , gama-Globulinas/análise , Proteínas Sanguíneas/análise , Eletroforese em Papel/métodos , JamaicaRESUMO
A method is proposed for the quantitation of globulin sub-fractions by paper electrophoresis which overcomes certain difficulties, mainly albumin trailing and the differential dye uptake between albumin and globulins. By this method the globulin sub-fractions can be quantitated with good reproducibility and the results obtained compare favourably with those obtained by the use of Cellulose acetate strips. The error involved in the quantitation of the Alpha globulins is so great that one should be very cautious in interpreting quantitative results of the Alpha globulins. On the other hand the Beta and Gamma can be quantitated with reasonable accuracy (AU)
Assuntos
Técnicas In Vitro , Globulinas/análise , Eletroforese em Papel/métodosRESUMO
The literature on abnormal haemoglobins is briefly reviewed. The laboratory findings on 39 cases of sickle cell anaemia (genotype S-S), 14 of whom were adults, and on 13 cases of sickle cell-haemoglobin C disease (genotype S-C), are presented and discussed. Two family studies are included, in one of which three cases of sickle cell-haemoglobin C disease were found. (AU)