RESUMO
High-throughput sequencing is a revolutionary technological innovation in DNA sequencing. This technology has an ultra-low cost per base of sequencing and an overwhelmingly high data output. High-throughput sequencing has brought novel research methods and solutions to the research fields of genomics and post-genomics. Furthermore, this technology is leading to a new molecular breeding revolution that has landmark significance for scientific research and enables us to launch multi-level, multi-faceted, and multi-extent studies in the fields of crop genetics, genomics, and crop breeding. In this paper, we review progress in the application of high-throughput sequencing technologies to plant molecular breeding studies.
Assuntos
Produtos Agrícolas/genética , Embaralhamento de DNA/tendências , Análise de Sequência de DNA/tendências , Variação Genética , Genoma de Planta , Mutação , Locos de Características QuantitativasRESUMO
Advances in the field of genomics and 'metagenomics' have dramatically revised our view of microbial biodiversity and its potential for biotechnological applications. Considering the estimation that >99% of microorganisms in most environments are not amenable to culturing, very little is known about their genomes, genes and encoded enzymatic activities. The isolation, archiving and analysis of environmental DNA (or so-called 'metagenomes') has enabled us to mine microbial diversity, allowing us to access their genomes, identify protein coding sequences and even to reconstruct biochemical pathways, providing insights into the properties and functions of these organisms. The generation and analysis of (meta)genomic libraries is thus a powerful approach to harvest and archive environmental genetic resources. It will enable us to identify which organisms are present, what they do, and how their genetic information can be beneficial to mankind.