RESUMO
Saint Louis encephalitis virus belongs to Flavivirus genus; Flaviviridae family jointly with other medically important flaviviruses including dengue virus and West Nile virus. The biological properties and functions of prM flavivirus protein are under investigation due to its importance in the generation of infectious virion and host interactions. Monoclonal antibodies have become powerful tools in this approach. Also the use of monoclonal antibodies has been successfully applied for antigenic analysis, clinical diagnosis and treatments. Here, using an immunofluorescence assay we describe a monoclonal antibody (mAb 3D2) that uniquely recognizes native prM Saint Louis encephalitis virus protein expressed in either C6/36-HT or Vero cells. In conclusion, mAb3D2 has significant potential for use in (a) the diagnosis of infections caused by this virus and (b) therapeutic use to treat patients infected by this virus and fundamental research to understand the role of the prM in the Saint Louis encephalitis virus infectious process.
Assuntos
Anticorpos Monoclonais/imunologia , Anticorpos Antivirais/imunologia , Vírus da Encefalite de St. Louis/imunologia , Encefalite de St. Louis , Proteínas do Envelope Viral/imunologia , Aedes , Animais , Anticorpos Monoclonais/uso terapêutico , Anticorpos Antivirais/uso terapêutico , Linhagem Celular , Chlorocebus aethiops , Encefalite de St. Louis/diagnóstico , Encefalite de St. Louis/terapia , Encefalite de St. Louis/virologia , Humanos , Células VeroAssuntos
Vírus da Encefalite de St. Louis/patogenicidade , Encefalite de St. Louis , Febre do Nilo Ocidental , Vírus do Nilo Ocidental/patogenicidade , Animais , Encefalite de St. Louis/diagnóstico , Encefalite de St. Louis/epidemiologia , Encefalite de St. Louis/terapia , Humanos , Febre do Nilo Ocidental/diagnóstico , Febre do Nilo Ocidental/epidemiologia , Febre do Nilo Ocidental/terapiaAssuntos
Infecções Bacterianas/microbiologia , Doenças Transmissíveis , Culicidae/virologia , Encefalite de St. Louis , Febre do Nilo Ocidental , Animais , Arkansas , Doenças Transmissíveis/diagnóstico , Doenças Transmissíveis/terapia , Doenças Transmissíveis/transmissão , Encefalite de St. Louis/diagnóstico , Encefalite de St. Louis/terapia , Encefalite de St. Louis/transmissão , Humanos , Mordeduras e Picadas de Insetos/microbiologia , Febre do Nilo Ocidental/diagnóstico , Febre do Nilo Ocidental/terapia , Febre do Nilo Ocidental/transmissãoRESUMO
BACKGROUND: St. Louis encephalitis (SLE) is a relatively common cause of neurological illness, yet little is known about its cognitive and psychosocial consequences. PURPOSE: To describe the cognitive, emotional, psychophysiological, and psychosocial consequences of SLE infection. METHOD: A comprehensive neuropsychological and psychophysiological evaluation of a high functioning woman 6 weeks and 1 year after acute SLE infection is presented. The focus and course of rehabilitation is also examined. RESULTS: The primary cognitive consequences of SLE infection involved attention, working memory, speed of processing, and cognitive efficiency. Depression was also observed. Psychometric testing suggested that these deficits largely resolved after 1 year. CONCLUSIONS: SLE produces neurocognitive deficits which are reflected in both psychometric and psychophysiologic measures and functional status. Psychometric and vocational improvement were observed over 1 year. However, the normal vocational return came at a significant psychosocial cost. This case emphasizes the importance of a comprehensive neuropsychological evaluation and illustrates the importance of an integrated rehabilitation programme.
