RESUMO
Creutzfeld-Jakob disease (CJD) is a fatal neurodegenerative disease which belongs to the family of transmissible spongiform encephalopathies (TSEs), or prion diseases. Historically, CJD diagnosis has been based on the combination of clinical features and in vivo markers, including CSF protein assays, MRI and EEG changes. Brain-derived CSF proteins, such as 14-3-3, t-tau and p-tau have been largely used to support the diagnosis of probable CJD, although with certain limitations concerning sensitivity and specificity of these tests. More recently, a new method for the pre-mortem diagnosis of sporadic CJD has been developed, based on the ability of PrPsc to induce the polymerization of protease-sensitive recombinant PrP (PrPsen) into amyloid fibrils, and is known as Real-Time Quaking- Induced Conversion (RT-QuIC) assay allows the detection of > 1 fg of PrPsc in diluted CJD brain homogenate and a variety of biological tissues and fluids. In the present study, we did a meta-analysis on the liability of RT-QuIC method in the diagnosis of sporadic CJD, in comparison to 14-3-3 and Tau protein. Twelve studies were finally included in the statistical analysis which showed that RT-QuIC has a very high specificity and comparable sensitivity to 14-3-3 protein and Tau protein in the CSF, and hence can be used as a reliable biomarker for the diagnosis of sporadic CJD.
Assuntos
Sistemas Computacionais/normas , Síndrome de Creutzfeldt-Jakob/diagnóstico por imagem , Síndrome de Creutzfeldt-Jakob/fisiopatologia , Encefalopatia Espongiforme Bovina/diagnóstico por imagem , Encefalopatia Espongiforme Bovina/fisiopatologia , Biomarcadores/líquido cefalorraquidiano , Síndrome de Creutzfeldt-Jakob/líquido cefalorraquidiano , Eletroencefalografia/métodos , Eletroencefalografia/normas , Encefalopatia Espongiforme Bovina/líquido cefalorraquidiano , Humanos , Imageamento por Ressonância Magnética/métodos , Imageamento por Ressonância Magnética/normasRESUMO
OBJECTIVE: To compare the diagnostic accuracy and the prognostic value of blood and cerebrospinal fluid (CSF) tests across prion disease subtypes. METHODS: We used a single-molecule immunoassay to measure tau and neurofilament light chain (NfL) protein levels in the plasma and assessed CSF total(t)-tau, NfL and protein 14-3-3 levels in patients with prion disease (n=336), non-prion rapidly progressive dementias (n=106) and non-neurodegenerative controls (n=37). We then evaluated each plasma and CSF marker for diagnosis and their association with survival, taking into account the disease subtype, which is a strong independent prognostic factor in prion disease. RESULTS: Plasma tau and NfL concentrations were higher in patients with prion disease than in non-neurodegenerative controls and non-prion rapidly progressive dementias. Plasma tau showed higher diagnostic value than plasma NfL, but a lower accuracy than the CSF proteins t-tau and 14-3-3. In the whole prion cohort, both plasma (tau and NfL) and CSF (t-tau, 14-3-3 and NfL) markers were significantly associated with survival and showed similar prognostic values. However, the intrasubtype analysis revealed that only CSF t-tau in sporadic Creutzfeldt-Jakob disease (sCJD) MM(V)1, plasma tau and CSF t-tau in sCJD VV2, and plasma NfL in slowly progressive prion diseases were significantly associated with survival after accounting for covariates. CONCLUSIONS: Plasma markers have lower diagnostic accuracy than CSF biomarkers. Plasma tau and NfL and CSF t-tau are significantly associated with survival in prion disease in a subtype-specific manner and can be used to improve clinical trial stratification and clinical care.
Assuntos
Proteínas 14-3-3/sangue , Síndrome de Creutzfeldt-Jakob/sangue , Síndrome de Creutzfeldt-Jakob/líquido cefalorraquidiano , Encefalopatia Espongiforme Bovina/sangue , Encefalopatia Espongiforme Bovina/líquido cefalorraquidiano , Proteínas de Neurofilamentos/sangue , Proteínas tau/sangue , Proteínas 14-3-3/líquido cefalorraquidiano , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/sangue , Biomarcadores/líquido cefalorraquidiano , Síndrome de Creutzfeldt-Jakob/classificação , Síndrome de Creutzfeldt-Jakob/diagnóstico , Demência/sangue , Demência/líquido cefalorraquidiano , Demência/diagnóstico , Progressão da Doença , Diagnóstico Precoce , Encefalopatia Espongiforme Bovina/classificação , Encefalopatia Espongiforme Bovina/diagnóstico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Proteínas de Neurofilamentos/líquido cefalorraquidiano , Doenças Priônicas/sangue , Doenças Priônicas/líquido cefalorraquidiano , Doenças Priônicas/classificação , Doenças Priônicas/diagnóstico , Prognóstico , Modelos de Riscos Proporcionais , Taxa de Sobrevida , Proteínas tau/líquido cefalorraquidianoAssuntos
Disfunção Cognitiva/diagnóstico , Síndrome de Creutzfeldt-Jakob/diagnóstico , Encefalite/diagnóstico , Encefalopatia Espongiforme Bovina/diagnóstico , Imageamento por Ressonância Magnética , Disfunção Cognitiva/etiologia , Síndrome de Creutzfeldt-Jakob/líquido cefalorraquidiano , Síndrome de Creutzfeldt-Jakob/complicações , Síndrome de Creutzfeldt-Jakob/fisiopatologia , Imagem de Difusão por Ressonância Magnética , Eletroencefalografia , Encefalite/complicações , Encefalite/imunologia , Encefalopatia Espongiforme Bovina/líquido cefalorraquidiano , Encefalopatia Espongiforme Bovina/complicações , Encefalopatia Espongiforme Bovina/fisiopatologia , Feminino , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/imunologia , Pessoa de Meia-Idade , Tomografia por Emissão de PósitronsRESUMO
Human prion diseases are classified into sporadic, genetic, and acquired forms. Within this last group, iatrogenic Creutzfeldt-Jakob disease (iCJD) is caused by human-to-human transmission through surgical and medical procedures. After reaching an incidence peak in the 1990s, it is believed that the iCJD historical period is probably coming to an end, thanks to lessons learnt from past infection sources that promoted new prion prevention and decontamination protocols. At this point, we sought to characterise the biomarker profile of iCJD and compare it to that of sporadic CJD (sCJD) for determining the value of available diagnostic tools in promptly recognising iCJD cases. To that end, we collected 23 iCJD samples from seven national CJD surveillance centres and analysed the electroencephalogram and neuroimaging data together with a panel of seven CSF biomarkers: 14-3-3, total tau, phosphorylated/total tau ratio, alpha-synuclein, neurofilament light, YKL-40, and real-time quaking induced conversion of prion protein. Using the cut-off values established for sCJD, we found the sensitivities of these biomarkers for iCJD to be similar to those described for sCJD. Given the limited relevant information on this issue to date, the present study validates the use of current sCJD biomarkers for the diagnosis of future iCJD cases.
Assuntos
Biomarcadores/líquido cefalorraquidiano , Síndrome de Creutzfeldt-Jakob/líquido cefalorraquidiano , Síndrome de Creutzfeldt-Jakob/diagnóstico por imagem , Encefalopatia Espongiforme Bovina/líquido cefalorraquidiano , Encefalopatia Espongiforme Bovina/diagnóstico por imagem , Doenças Priônicas/líquido cefalorraquidiano , Doenças Priônicas/diagnóstico por imagem , Proteínas Priônicas/metabolismo , Adulto , Idoso , Transplante de Córnea/efeitos adversos , Síndrome de Creutzfeldt-Jakob/epidemiologia , Dura-Máter/transplante , Eletroencefalografia , Encefalopatia Espongiforme Bovina/epidemiologia , Feminino , Homozigoto , Hormônio do Crescimento Humano/efeitos adversos , Humanos , Doença Iatrogênica , Estimativa de Kaplan-Meier , Imageamento por Ressonância Magnética , Masculino , Metionina/genética , Pessoa de Meia-Idade , Neuroimagem , Fenótipo , Polimorfismo Genético , Sistema de Registros , Reprodutibilidade dos Testes , Fatores Sexuais , Fatores de TempoRESUMO
Since 2005, two cases of natural bovine spongiform encephalopathies (BSE) have been reported in goats. Furthermore, experimental transmissions of classical (C-BSE) and atypical (L-BSE) forms of BSE in goats were also reported. To minimize further spreading of prion diseases in small ruminants the development of a highly sensitive and specific test for ante-mortem detection of infected animals would be of great value. Recent studies reported high diagnostic value of a second generation of cerebrospinal fluid (CSF) Real-Time Quaking-Induced Conversion (RT-QuIC) assay across a wide spectrum of human prions. Here, we applied this improved QuIC (IQ-CSF) for highly efficient detection of TSEs prion protein in goat cerebrospinal fluid. IQ-CSF sensitivity and specificity were evaluated on CSF samples collected at disease endpoint from goats naturally and experimentally infected with scrapie or bovine isolates of C-BSE and L-BSE, respectively. Next, CSF samples collected from L-BSE infected goats during pre-symptomatic stage were also analysed. PrPL-BSE associated seeding activity was detected at early time points after experimental inoculation, with an average time of 439 days before clinical symptoms appeared. Taken together these data are indicative of the great potential of this in vitro prion amplification assay as ante-mortem TSE test for live and asymptomatic small ruminants.
Assuntos
Encefalopatia Espongiforme Bovina/líquido cefalorraquidiano , Doenças das Cabras/líquido cefalorraquidiano , Cabras/líquido cefalorraquidiano , Príons/isolamento & purificação , Animais , Encéfalo/patologia , Bovinos , Encefalopatia Espongiforme Bovina/diagnóstico , Doenças das Cabras/diagnóstico , Humanos , Proteínas PrPSc/isolamento & purificaçãoRESUMO
Prion diseases are a group of fatal neurodegenerative disorders that affect humans and animals. Besides of the pathological agent, prion, there are some elements that can influence or determine susceptibility to prion infection and the clinical phenotype of the diseases, e.g., the polymorphism in PRNP gene. Another polymorphism in ZBTB38-RASA2 has been observed to be associated with the susceptibility of sporadic Creutzfeldt-Jacob disease (sCJD) in UK. MicroRNAs are endogenous small noncoding RNAs that control gene expression by targeting mRNAs and triggering either translation repression or RNA degradation. In this study, two polymorphic loci in miR-146a (rs2910164 and rs57095329) and one locus in ZBTB38-RASA2 (rs295301) of 561 Chinese patients of sCJD and 31 cases of fatal familial insomnia (FFI) were screened by PCR and sequencing. Our data did not figure out any association of those three SNPs with the susceptibility of sCJD. However, a significant association of the SNP of rs57095329 in miR-146a showed the association with the susceptibility of FFI. Additionally, the SNP of rs57095329 showed statistical significances with the appearances of mutism and the positive of cerebrospinal fluid (CSF) protein 14-3-3 in sCJD patients, while the SNP of ZBTB38-RASA2 was significantly related with the appearance of myoclonus in sCJD patients. It indicates that the SNPs of ZBTB38-RASA2 and miR-146a are not associated with the susceptibility of the Chinese sCJD patients, but may influence the appearances of clinical manifestations somehow.
Assuntos
Síndrome de Creutzfeldt-Jakob/genética , Encefalopatia Espongiforme Bovina/genética , Insônia Familiar Fatal/genética , MicroRNAs/genética , Polimorfismo de Nucleotídeo Único , Proteínas Repressoras/genética , Proteínas Ativadoras de ras GTPase/genética , Proteínas 14-3-3/genética , Idoso , Povo Asiático , Síndrome de Creutzfeldt-Jakob/sangue , Síndrome de Creutzfeldt-Jakob/líquido cefalorraquidiano , Síndrome de Creutzfeldt-Jakob/diagnóstico , Suscetibilidade a Doenças , Encefalopatia Espongiforme Bovina/sangue , Encefalopatia Espongiforme Bovina/líquido cefalorraquidiano , Encefalopatia Espongiforme Bovina/diagnóstico , Frequência do Gene , Genótipo , Humanos , Insônia Familiar Fatal/sangue , Insônia Familiar Fatal/líquido cefalorraquidiano , Insônia Familiar Fatal/diagnóstico , Pessoa de Meia-Idade , Mioclonia/genética , Razão de ChancesRESUMO
Prion diseases are characterized by the prominent accumulation of the misfolded form of a normal cellular protein (PrP(Sc)) in the central nervous system. The pathological features and biochemical properties of PrP(Sc) in macaque monkeys infected with the bovine spongiform encephalopathy (BSE) prion have been found to be similar to those of human subjects with variant Creutzfeldt-Jakob disease (vCJD). Non-human primate models are thus ideally suited for performing valid diagnostic tests and determining the efficacy of potential therapeutic agents. In the current study, we developed a highly efficient method for in vitro amplification of cynomolgus macaque BSE PrP(Sc). This method involves amplifying PrP(Sc) by protein misfolding cyclic amplification (PMCA) using mouse brain homogenate as a PrP(C) substrate in the presence of sulfated dextran compounds. This method is capable of amplifying very small amounts of PrP(Sc) contained in the cerebrospinal fluid (CSF) and white blood cells (WBCs), as well as in the peripheral tissues of macaques that have been intracerebrally inoculated with the BSE prion. After clinical signs of the disease appeared in three macaques, we detected PrP(Sc) in the CSF by serial PMCA, and the CSF levels of PrP(Sc) tended to increase with disease progression. In addition, PrP(Sc) was detectable in WBCs at the clinical phases of the disease in two of the three macaques. Thus, our highly sensitive, novel method may be useful for furthering the understanding of the tissue distribution of PrP(Sc) in non-human primate models of CJD.
Assuntos
Encefalopatia Espongiforme Bovina/sangue , Encefalopatia Espongiforme Bovina/líquido cefalorraquidiano , Macaca fascicularis/sangue , Macaca fascicularis/líquido cefalorraquidiano , Proteínas PrPSc/sangue , Proteínas PrPSc/líquido cefalorraquidiano , Animais , Bovinos , Síndrome de Creutzfeldt-Jakob/sangue , Síndrome de Creutzfeldt-Jakob/líquido cefalorraquidiano , Modelos Animais de Doenças , Humanos , Masculino , Camundongos , Distribuição TecidualRESUMO
The shotgun strategy applying tandem mass spectrometry has been widely used to identify the proteins that are differentially distributed among diseases for its high reliability and efficiency. To find out the potential difference of protein profiles in cerebrospinal fluids (CSF) between Creutzfeldt-Jakob disease (CJD) and non-CJD patients, especially in the fraction ranging from 1-10 KD, the CSF samples of 40 probable sporadic CJD (sCJD) patients, 32 non-CJD cases with dementia and 17 non-CJD cases without dementia were separately pooled and enriched by the magnetic beads based weak cation exchange chromatography (MB-WCX). After trypsin digestion, each enriched CSF was separated and identified by RP-HPLC-ESI-QTOF MS/MS. In total, 42, 53 and 47 signals of proteins were identified in the pooled CSF fraction less than 10 KD of probable sCJD, non-CJD with dementia and non-CJD without dementia, respectively. Compared with that of probable sCJD, the similarity of CSF protein profiles of non-CJD with dementia (76.2%) were higher than that of non-CJD without dementia (57.1%). Nine CSF proteins were found to be specially observed in probable sCJD group. Those data may help to select the potential biomarkers for diagnosis of CJD. Additionally, further studies of the small segments of cellular proteins in CSF of CJD patients may also provide scientific clues for understanding the neuropathogenesis of TSEs.
Assuntos
Síndrome de Creutzfeldt-Jakob/líquido cefalorraquidiano , Encefalopatia Espongiforme Bovina/líquido cefalorraquidiano , Peptídeos/líquido cefalorraquidiano , Peptídeos/química , Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Espectrometria de Massas , Pessoa de Meia-Idade , Peso Molecular , Adulto JovemRESUMO
Bovine spongiform encephalopathy (BSE) and variant Creutzfeldt-Jakob disease (vCJD) are two new members of the family of neurodegenerative conditions termed prion diseases. Oxidative damage has been shown to occur in prion diseases and is potentially responsible for the rapid neurodegeneration that is central to the pathogenesis of these diseases. An important nonenzymatic antioxidant in the brain is uric acid. Analysis of uric acid in the brain and cerebrospinal fluid (CSF) of cases of BSE and CJD showed a specific reduction in CSF levels for both BSE and variant CJD, but not sporadic CJD. Further studies based on cell culture experiments suggested that uric acid in the brain was produced by microglia. Uric acid was also shown to inhibit neurotoxicity of a prion protein peptide, production of the abnormal prion protein isoform (PrP(Sc)) by infected cells, and polymerization of recombinant prion protein. These findings suggest that changes in uric acid may aid differential diagnosis of vCJD. Uric acid could be used to inhibit cell death or PrP(Sc) formation in prion disease.
Assuntos
Encéfalo/metabolismo , Síndrome de Creutzfeldt-Jakob/metabolismo , Encefalopatia Espongiforme Bovina/metabolismo , Ácido Úrico/metabolismo , Animais , Química Encefálica , Bovinos , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Síndrome de Creutzfeldt-Jakob/líquido cefalorraquidiano , Relação Dose-Resposta a Droga , Encefalopatia Espongiforme Bovina/líquido cefalorraquidiano , Humanos , Microglia/metabolismo , Microglia/patologia , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Neurônios/patologia , Fragmentos de Peptídeos/antagonistas & inibidores , Fragmentos de Peptídeos/farmacologia , Proteínas PrPSc/biossíntese , Príons/antagonistas & inibidores , Príons/farmacologia , Ácido Úrico/análise , Ácido Úrico/farmacologiaAssuntos
Encefalopatia Espongiforme Bovina/líquido cefalorraquidiano , Proteínas S100/líquido cefalorraquidiano , Animais , Biomarcadores , Bovinos , Diagnóstico Diferencial , Encefalopatia Espongiforme Bovina/diagnóstico , Fatores de Crescimento Neural , Subunidade beta da Proteína Ligante de Cálcio S100 , Sensibilidade e EspecificidadeRESUMO
The cerebrospinal fluid (CSF) of patients suffering from Creutzfeldt-Jakob disease (CJD) display two unique polypeptide chains by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). In the absence of a well-defined ante-mortem diagnostic test for bovine spongiform encephalopathy (BSE), spinal fluid samples of eight normal cows and eight cows known to carry BSE by post-mortem histological analysis were investigated to verify if equivalent polypeptides were present. Proteins with similar migration to human CJD polypeptides were not detected. But surprisingly, a cluster of polypeptide spots that was faint or not detected in normal bovine CSF samples was found to be elevated or massively increased in BSE CSF samples (more than 10-fold increase). These elevated polypeptide chains were identified as apolipoprotein E.
Assuntos
Apolipoproteínas E/líquido cefalorraquidiano , Síndrome de Creutzfeldt-Jakob/líquido cefalorraquidiano , Encefalopatia Espongiforme Bovina/líquido cefalorraquidiano , Sequência de Aminoácidos , Animais , Apolipoproteínas E/química , Bovinos , Eletroforese em Gel Bidimensional , Eletroforese em Gel de Poliacrilamida , Humanos , Dados de Sequência Molecular , Fragmentos de Peptídeos/química , Valores de Referência , Reprodutibilidade dos Testes , Especificidade da EspécieAssuntos
Síndrome de Creutzfeldt-Jakob/diagnóstico , Encefalopatia Espongiforme Bovina/diagnóstico , Adulto , Animais , Síndrome de Creutzfeldt-Jakob/líquido cefalorraquidiano , Eletroforese em Gel Bidimensional , Encefalopatia Espongiforme Bovina/líquido cefalorraquidiano , Humanos , Imunoensaio , Príons/líquido cefalorraquidianoRESUMO
Two-dimensional gel electrophoresis was used to analyse cerebrospinal fluid (CSF) from 75 suspect cases of bovine spongiform encephalopathy (BSE), 61 of which were confirmed by post mortem brain histopathology, and 38 normal cattle. CSF samples were also examined from cattle killed at periodic intervals through the incubation period following experimental challenge. Consistent changes were recorded in all CSF samples from the confirmed cases of natural BSE and also from cattle showing early signs of experimental disease. The changes consisted of an increased intensity of staining of apolipoprotein E and the presence of two protein spots, as yet unidentified, of molecular weights 35 and 36 kDa, both with a pI of 5.5. These changes were absent in the CSF samples from the normal cattle, from the clinically suspect cattle which were not confirmed as BSE and from the experimentally challenged cattle in the preclinical phase of infection.