RESUMO
Microsporidia are eukaryotic, intracellular obligate parasites that infect invertebrate and vertebrate animals, and have emerged as important opportunistic parasites in AIDS patients. We used light microscopy to detect microsporidial spores in stool samples of a domestic cat confirmed as Encephalitozoon intestinalis by PCR, owned by an AIDS patient with chronic diarrhea and E. intestinalis infection. Cats can be considered hosts of E. intestinalis.
Assuntos
Síndrome da Imunodeficiência Adquirida/complicações , Doenças do Gato/microbiologia , Encephalitozoon/classificação , Encefalitozoonose/veterinária , Albendazol/uso terapêutico , Animais , Anti-Helmínticos/uso terapêutico , Gatos , Encefalitozoonose/complicações , Encefalitozoonose/tratamento farmacológico , Encefalitozoonose/microbiologia , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
We describe a new microsporidian species, Encephalitozoon romaleae n. sp., isolated from an invertebrate host, the grasshopper Romalea microptera, collected near Weeks Island, Louisiana, and Jacksonville, Florida. This microsporidian is characterized by specificity to the gastric caecae and midgut tissues of the host and a life cycle that is nearly identical to that of Encephalitozoon hellem and Encephalitozoon cuniculi. Mature spores are larger (3.97 x 1.95 microm) than those of other Encephalitozoon species. Polar filament coils number 7 to 8 in a single row. Analysis of the small subunit (SSU) rDNA shows that E. romaleae fits well into the Encephalitozoon group and is a sister taxon to E. hellem. This is the first Encephalitozoon species that has been shown to complete its life cycle in an invertebrate host.
Assuntos
Encephalitozoon/classificação , Gafanhotos/parasitologia , Animais , DNA Fúngico/química , DNA Ribossômico/química , Encephalitozoon/genética , Encephalitozoon/ultraestrutura , Microscopia Eletrônica de Transmissão , Microscopia de Contraste de Fase , FilogeniaRESUMO
A Encephalitozoon-like microsporidia was found in epithelial cells of the midgut and the salivary glands of Amblyomma cajennense (F.) and Anocentor nitens (Neumann) that had fed on rabbits. Ultrastructural studies demonstrated that all stages of the life cycle of the parasite occur in parasitophorous vacuoles and contain only 1 nucleus. The sporonts detach from the limiting membrane of the vacuole and divide by binary fission to produce the sporoblasts, each presenting a thickened electron-dense wall, and a primordium of a polar filament. Each spore contained a single nucleus, an electron-dense and rough exospore, an electron-lucent and thick endospore, and 5 coils of the polar tubule.