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1.
Protein Expr Purif ; 114: 115-20, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26118698

RESUMO

The (3R)-hydroxyacyl-ACP dehydratase HadAB, involved in the biosynthetic pathway for mycolic acid (MA) of Mycobacterium tuberculosis, catalyzes the third step in the fatty acid (FA) elongation cycle, which is an ideal and actual target for anti-tubercular agent. Though HadAB is predicted to be a member of the hotdog superfamily, it shares no sequence identity with typical hotdog fold isoenzyme FabZ. To characterize the significance of HadAB from the perspective of structural biology, large amount of pure HadAB complex is required for biochemical characterization and crystallization. Here, we used a unique expression and purification method. HadA and HadB were cloned separately and co-expressed in Escherichia coli. After GST affinity chromatography, two steps of anion exchange chromatography and gel filtration, the purity of the protein as estimated by SDS-PAGE was >95%. Using hanging-drop vapor-diffusion method, crystals were obtained and diffracted X-rays to 1.75Å resolution. The crystal belongs to space group P41212, with unit-cell parameters a=b=82.0Å, c=139.8Å, α=ß=γ=90.0°.


Assuntos
Proteínas de Bactérias/metabolismo , Enoil-CoA Hidratase/metabolismo , Mycobacterium tuberculosis/enzimologia , Proteínas Recombinantes/metabolismo , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Proteínas de Bactérias/isolamento & purificação , Clonagem Molecular , Cristalização , Enoil-CoA Hidratase/química , Enoil-CoA Hidratase/genética , Enoil-CoA Hidratase/isolamento & purificação , Escherichia coli/genética , Mycobacterium tuberculosis/genética , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/isolamento & purificação
2.
Appl Microbiol Biotechnol ; 97(11): 4821-9, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23474615

RESUMO

A polyhydroxyalkanoate (PHA) was enzymatically synthesized in vitro, and the end structure of PHA associated with a chain transfer (CT) reaction was investigated. In the CT reaction, PHA chain transfers from PHA synthase (PhaC) to a CT agent, resulting in covalent bonding of CT agent to the PHA chain at its carboxyl end. In vitro CT reaction has never been demonstrated because of relatively low yields of in vitro synthesized poly[(R)-3-hydroxybutyrate)] (P(3HB)), which makes it difficult to characterize the end structures of the polymers by nuclear magnetic resonance (NMR). To overcome these difficulties, a novel in vitro synthesis method that produced relatively larger amounts of P(3HB) was developed by employing PhaCDa from Delftia acidovorans and two enantioselective enoyl-coenzyme A (CoA) hydratases which were R-hydratase (PhaJAc) from Aeromonas caviae and S-hydratase (FadB1x) from Pseudomonas putida KT2440 with ß-butyrolactone and CoA as starting materials. Using this method, P(3HB) synthesis was performed with tetraethylene glycols (TEGs) as a discriminable CT agent, and the resultant P(3HB) was characterized by (1)H-NMR. NMR analysis revealed that the carboxylic end of P(3HB) was covalently linked to TEGs, providing the first direct evidence of in vitro CT reaction.


Assuntos
Aciltransferases/metabolismo , Aeromonas caviae/enzimologia , Delftia acidovorans/enzimologia , Enoil-CoA Hidratase/metabolismo , Polietilenoglicóis/metabolismo , Poli-Hidroxialcanoatos/metabolismo , Pseudomonas putida/enzimologia , Aciltransferases/isolamento & purificação , Enoil-CoA Hidratase/isolamento & purificação , Polimerização
3.
Biosci Biotechnol Biochem ; 76(3): 613-6, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22451412

RESUMO

The (R)-specific enoyl-CoA hydratase gene (phaJ(HS21)) from Pseudomonas chlororaphis HS21 was overexpressed in various Pseudomonas strains, alone and in combination with the polyhydroxyalkanoate synthase gene (phaC(HS21)), for the biosynthesis of polyhydroxyalkanoates (PHAs) of altered monomer composition. Recombinant Pseudomonas strains harboring phaC(HS21) and phaJ(HS21) generated saturated and unsaturated monomers of C12-C14 in their PHAs. In particular, the level of the 3-hydroxytetradecenoate monomer in recombinant P. chlororaphis HS21 increased by approximately 260%. PhaJ(HS21) is expected to be useful in the biosynthesis of PHAs consisting of unusual monomer units.


Assuntos
Enoil-CoA Hidratase/genética , Enoil-CoA Hidratase/metabolismo , Poli-Hidroxialcanoatos/biossíntese , Poli-Hidroxialcanoatos/química , Pseudomonas/genética , Pseudomonas/metabolismo , Enoil-CoA Hidratase/isolamento & purificação , Expressão Gênica , Pseudomonas/enzimologia , Estereoisomerismo , Especificidade por Substrato
4.
Appl Microbiol Biotechnol ; 90(3): 951-9, 2011 May.
Artigo em Inglês | MEDLINE | ID: mdl-21327961

RESUMO

We investigated the expression of (R)-specific enoyl coenzyme A hydratase (PhaJ) in Pseudomonas putida KT2440 accumulating polyhydroxyalkanoate (PHA) from sodium octanoate in order to identify biosynthesis pathways of PHAs from fatty acids in pseudomonads. From a database search through the P. putida KT2440 genome, an additional phaJ gene homologous to phaJ4(Pa) from Pseudomonas aeruginosa, termed phaJ4(Pp), was identified. The gene products of phaJ1(Pp), which was identified previously, and phaJ4(Pp) were confirmed to be functional in recombinant Escherichia coli on PHA synthesis from sodium dodecanoate. Cytosolic proteins from P. putida grown on sodium octanoate were subjected to anion exchange chromatography and one of the eluted fractions with hydratase activity included PhaJ4(Pp), as revealed by western blot analysis. These results strongly suggest that PhaJ4(Pp) forms a channeling route from ß-oxidation to PHA biosynthesis in P. putida. Moreover, the substrate specificity of PhaJ1(Pp) was suggested to be different from that of PhaJ1(Pa) from P. aeruginosa although these two proteins share 67% amino acid sequence identity.


Assuntos
Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Enoil-CoA Hidratase/química , Enoil-CoA Hidratase/genética , Expressão Gênica , Poli-Hidroxialcanoatos/biossíntese , Pseudomonas putida/enzimologia , Proteínas de Bactérias/isolamento & purificação , Proteínas de Bactérias/metabolismo , Caprilatos/química , Caprilatos/metabolismo , Enoil-CoA Hidratase/isolamento & purificação , Enoil-CoA Hidratase/metabolismo , Escherichia coli/genética , Escherichia coli/metabolismo , Dados de Sequência Molecular , Oxirredução , Pseudomonas putida/química , Pseudomonas putida/genética , Pseudomonas putida/metabolismo , Especificidade por Substrato
5.
J Bacteriol ; 191(14): 4572-81, 2009 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-19429610

RESUMO

A 3-hydroxypropionate/4-hydroxybutyrate cycle operates in autotrophic CO(2) fixation in various Crenarchaea, as studied in some detail in Metallosphaera sedula. This cycle and the autotrophic 3-hydroxypropionate cycle in Chloroflexus aurantiacus have in common the conversion of acetyl-coenzyme A (CoA) and two bicarbonates via 3-hydroxypropionate to succinyl-CoA. Both cycles require the reductive conversion of 3-hydroxypropionate to propionyl-CoA. In M. sedula the reaction sequence is catalyzed by three enzymes. The first enzyme, 3-hydroxypropionyl-CoA synthetase, catalyzes the CoA- and MgATP-dependent formation of 3-hydroxypropionyl-CoA. The next two enzymes were purified from M. sedula or Sulfolobus tokodaii and studied. 3-Hydroxypropionyl-CoA dehydratase, a member of the enoyl-CoA hydratase family, eliminates water from 3-hydroxypropionyl-CoA to form acryloyl-CoA. Acryloyl-CoA reductase, a member of the zinc-containing alcohol dehydrogenase family, reduces acryloyl-CoA with NADPH to propionyl-CoA. Genes highly similar to the Metallosphaera CoA synthetase, dehydratase, and reductase genes were found in autotrophic members of the Sulfolobales. The encoded enzymes are only distantly related to the respective three enzyme domains of propionyl-CoA synthase from C. aurantiacus, where this trifunctional enzyme catalyzes all three reactions. This indicates that the autotrophic carbon fixation cycles in Chloroflexus and in the Sulfolobales evolved independently and that different genes/enzymes have been recruited in the two lineages that catalyze the same kinds of reactions.


Assuntos
Acil Coenzima A/metabolismo , Proteínas Arqueais/metabolismo , Enoil-CoA Hidratase/metabolismo , Hidroxibutiratos/metabolismo , Oxirredutases/metabolismo , Propionatos/metabolismo , Sulfolobales/enzimologia , Proteínas Arqueais/isolamento & purificação , Enoil-CoA Hidratase/genética , Enoil-CoA Hidratase/isolamento & purificação , Genes Arqueais , Redes e Vias Metabólicas , Modelos Biológicos , NADP/metabolismo , Oxirredutases/genética , Oxirredutases/isolamento & purificação , Homologia de Sequência de Aminoácidos , Sulfolobales/genética
6.
Artigo em Inglês | MEDLINE | ID: mdl-18453716

RESUMO

The assimilation of aromatic compounds by microbial species requires specialized enzymes to cleave the thermodynamically stable ring. In the recently discovered benzoate-oxidation (box) pathway in Burkholderia xenovorans LB400, this is accomplished by a novel dihydrodiol lyase (BoxC(C)). Sequence analysis suggests that BoxC(C) is part of the crotonase superfamily but includes an additional uncharacterized region of approximately 115 residues that is predicted to mediate ring cleavage. Processing of X-ray diffraction data to 1.5 A resolution revealed that BoxC(C) crystallized with two molecules in the asymmetric unit of the P2(1)2(1)2(1) space group, with a solvent content of 47% and a Matthews coefficient of 2.32 A(3) Da(-1). Selenomethionine BoxC(C) has been purified and crystals are currently being refined for anomalous dispersion studies.


Assuntos
Proteínas de Bactérias/química , Burkholderia/enzimologia , Enoil-CoA Hidratase/química , Proteínas de Bactérias/isolamento & purificação , Proteínas de Bactérias/metabolismo , Clonagem Molecular , Cristalização/métodos , Enoil-CoA Hidratase/isolamento & purificação , Enoil-CoA Hidratase/metabolismo , Difração de Raios X
7.
Arch Microbiol ; 183(3): 176-89, 2005 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-15731894

RESUMO

Enzymes involved in carnitine metabolism of Proteus sp. are encoded by the cai genes organised as the caiTABCDEF operon. The complete operon could be sequenced from the genomic DNA of Proteus sp. Amino acid sequence similarities and/or enzymatic analysis confirmed the function assigned to each protein involved in carnitine metabolism. CaiT was suggested to be an integral membrane protein responsible for the transport of betaines. The caiA gene product was shown to be a crotonobetainyl-CoA reductase catalysing the irreversible reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. CaiB and CaiD were identified to be the two components of the crotonobetaine hydrating system, already described. CaiB and caiD were cloned and expressed in Escherichia coli. After purification of both proteins, their individual enzymatic functions were solved. CaiB acts as betainyl-CoA transferase specific for carnitine, crotonobetaine, gamma-butyrobetaine and its CoA derivatives. Transferase reaction proceeds, following a sequential bisubstrate mechanism. CaiD was identified to be a crotonobetainyl-CoA hydratase belonging to the crotononase superfamily. Because of amino acid sequence similarities, CaiC was suggested to be a betainyl-CoA ligase. Taken together, these results show that the metabolism of carnitine and crotonobetaine in Proteus sp. proceeds at the CoA level.


Assuntos
Betaína/análogos & derivados , Carnitina/metabolismo , Genes Bacterianos , Proteus/enzimologia , Proteus/genética , Acil Coenzima A/metabolismo , Aciltransferases/genética , Aciltransferases/isolamento & purificação , Aciltransferases/metabolismo , Antiporters/genética , Proteínas de Bactérias/genética , Proteínas de Bactérias/isolamento & purificação , Proteínas de Bactérias/metabolismo , Betaína/metabolismo , Clonagem Molecular , Coenzima A Ligases/genética , Coenzima A Ligases/isolamento & purificação , Coenzima A Ligases/metabolismo , DNA Bacteriano/química , Enoil-CoA Hidratase/genética , Enoil-CoA Hidratase/isolamento & purificação , Enoil-CoA Hidratase/metabolismo , Escherichia coli/genética , Escherichia coli/metabolismo , Dados de Sequência Molecular , Óperon , Oxirredutases/genética , Oxirredutases/isolamento & purificação , Oxirredutases/metabolismo , Proteínas Recombinantes/genética , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Especificidade por Substrato
8.
J Mol Biol ; 345(5): 1157-69, 2005 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-15644212

RESUMO

2-Enoyl-CoA hydratase 2 is the middle part of the mammalian peroxisomal multifunctional enzyme type 2 (MFE-2), which is known to be important in the beta-oxidation of very-long-chain and alpha-methyl-branched fatty acids as well as in the synthesis of bile acids. Here, we present the crystal structure of the hydratase 2 from the human MFE-2 to 3A resolution. The three-dimensional structure resembles the recently solved crystal structure of hydratase 2 from the yeast, Candida tropicalis, MFE-2 having a two-domain subunit structure with a C-domain complete hot-dog fold housing the active site, and an N-domain incomplete hot-dog fold housing the cavity for the aliphatic acyl part of the substrate molecule. The ability of human hydratase 2 to utilize such bulky compounds which are not physiological substrates for the fungal ortholog, e.g. CoA esters of C26 fatty acids, pristanic acid and di/trihydroxycholestanoic acids, is explained by a large hydrophobic cavity formed upon the movements of the extremely mobile loops I-III in the N-domain. In the unliganded form of human hydratase 2, however, the loop I blocks the entrance of fatty enoyl-CoAs with chain-length >C8. Therefore, we expect that upon binding of substrates bulkier than C8, the loop I gives way, contemporaneously causing a secondary effect in the CoA-binding pocket and/or active site required for efficient hydration reaction. This structural feature would explain the inactivity of human hydratase 2 towards short-chain substrates. The solved structure is also used as a tool for analyzing the various inactivating mutations, identified among others in MFE-2-deficient patients. Since hydratase 2 is the last functional unit of mammalian MFE-2 whose structure has been solved, the organization of the functional units in the biologically active full-length enzyme is also discussed.


Assuntos
Enoil-CoA Hidratase/química , Enoil-CoA Hidratase/metabolismo , Peroxissomos/enzimologia , Sequência de Aminoácidos , Sítios de Ligação , Cristalografia por Raios X , Enoil-CoA Hidratase/genética , Enoil-CoA Hidratase/isolamento & purificação , Humanos , Ligantes , Modelos Moleculares , Dados de Sequência Molecular , Mutação/genética , Peroxissomos/genética , Conformação Proteica , Alinhamento de Sequência
9.
Appl Environ Microbiol ; 69(8): 4830-6, 2003 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-12902277

RESUMO

Aeromonas caviae R-specific enoyl-coenzyme A (enoyl-CoA) hydratase (PhaJ(Ac)) is capable of providing (R)-3-hydroxyacyl-CoA with a chain length of four to six carbon atoms from the fatty acid beta-oxidation pathway for polyhydroxyalkanoate (PHA) synthesis. In this study, amino acid substitutions were introduced into PhaJ(Ac) by site-directed mutagenesis to investigate the feasibility of altering the specificity for the acyl chain length of the substrate. A crystallographic structure analysis of PhaJ(Ac) revealed that Ser-62, Leu-65, and Val-130 define the width and depth of the acyl-chain-binding pocket. Accordingly, we targeted these three residues for amino acid substitution. Nine single-mutation enzymes and two double-mutation enzymes were generated, and their hydratase activities were assayed in vitro by using trans-2-octenoyl-CoA (C(8)) as a substrate. Three of these mutant enzymes, L65A, L65G, and V130G, exhibited significantly high activities toward octenoyl-CoA than the wild-type enzyme exhibited. PHA formation from dodecanoate (C(12)) was examined by using the mutated PhaJ(Ac) as a monomer supplier in recombinant Escherichia coli LS5218 harboring a PHA synthase gene from Pseudomonas sp. strain 61-3 (phaC1(Ps)). When L65A, L65G, or V130G was used individually, increased molar fractions of 3-hydroxyoctanoate (C(8)) and 3-hydroxydecanoate (C(10)) units were incorporated into PHA. These results revealed that Leu-65 and Val-130 affect the acyl chain length substrate specificity. Furthermore, comparative kinetic analyses of the wild-type enzyme and the L65A and V130G mutants were performed, and the mechanisms underlying changes in substrate specificity are discussed.


Assuntos
Aeromonas/enzimologia , Enoil-CoA Hidratase/química , Enoil-CoA Hidratase/metabolismo , Dicroísmo Circular , Enoil-CoA Hidratase/isolamento & purificação , Estabilidade Enzimática , Cinética , Mutagênese Sítio-Dirigida , Mutação , Poliésteres/metabolismo , Relação Estrutura-Atividade , Especificidade por Substrato
10.
Acta Crystallogr D Biol Crystallogr ; 59(Pt 7): 1302-5, 2003 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-12832794

RESUMO

In yeast, the second and the third reaction of the fatty-acid beta-oxidation spiral are catalysed by peroxisomal multifunctional enzyme type 2 (Mfe2p/Fox2p). This protein has two (3R)-hydroxyacyl-CoA dehydrogenase domains and a C-terminal 2-enoyl-CoA hydratase 2 domain. Here, the purification, crystallization and X-ray diffraction analysis of the hydratase 2 domain [CtMfe2p(dh(a+b)Delta)] from Candida tropicalis Mfe2p is reported. CtMfe2p(dh(a+b)Delta) was overexpressed as an enzymatically active recombinant protein and crystallized by the hanging-drop vapour-diffusion method. The crystals belong to space group C2, with unit-cell parameters a = 178.57, b = 60.46, c = 130.85 A, beta = 94.48 degrees. Selenomethionine-labelled protein was used for a multi-wavelength anomalous dispersion (MAD) experiment. A three-wavelength data set suitable for MAD phasing was collected to 2.25 A resolution using synchrotron radiation.


Assuntos
Candida tropicalis/enzimologia , Enoil-CoA Hidratase/química , Clonagem Molecular , Cristalização/métodos , Cristalografia por Raios X/métodos , Enoil-CoA Hidratase/genética , Enoil-CoA Hidratase/isolamento & purificação , Proteínas Fúngicas/química , Estrutura Terciária de Proteína , Selenometionina
11.
Plant Physiol ; 131(2): 753-62, 2003 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-12586899

RESUMO

The biochemical and molecular properties of the beta-oxidation enzymes from algae have not been investigated yet. The present study provides such data for the phylogenetically old alga Euglena (Euglena gracilis). A novel multifunctional beta-oxidation complex was purified to homogeneity by ammonium sulfate precipitation, density gradient centrifugation, and ion-exchange chromatography. Monospecific antibodies used in immunocytochemical experiments revealed that the enzyme is located in mitochondria. The enzyme complex is composed of 3-hydroxyacyl-coenzyme A (-CoA) dehydrogenase, 2-enoyl-CoA hydratase, thiolase, and epimerase activities. The purified enzyme exhibits a native molecular mass of about 460 kD, consisting of 45.5-, 44.5-, 34-, and 32-kD subunits. Subunits dissociated from the complete complex revealed that the hydratase and the thiolase functions are located on the large subunits, whereas two dehydrogenase functions are located on the two smaller subunits. Epimerase activity was only measurable in the complete enzyme complex. From the use of stereoisomers and sequence data, it was concluded that the 2-enoyl-CoA hydratase catalyzes the formation of L-hydroxyacyl CoA isomers and that both of the different 3-hydroxyacyl-CoA dehydrogenase functions on the 32- and 34-kD subunits are specific to L-isomers as substrates, respectively. All of these data suggest that the Euglena enzyme belongs to the family of beta-oxidation enzymes that degrade acyl-CoAs via L-isomers and that it is composed of subunits comparable with subunits of monofunctional beta-oxidation enzymes. It is concluded that the Euglena enzyme phylogenetically developed from monospecific enzymes in archeons by non-covalent combination of subunits and presents an additional line for the evolutionary development of multifunctional beta-oxidation enzymes.


Assuntos
Proteínas de Algas/metabolismo , Euglena/enzimologia , Complexos Multienzimáticos/metabolismo , 3-Hidroxiacil-CoA Desidrogenases/genética , 3-Hidroxiacil-CoA Desidrogenases/isolamento & purificação , 3-Hidroxiacil-CoA Desidrogenases/metabolismo , Acetil-CoA C-Acetiltransferase/genética , Acetil-CoA C-Acetiltransferase/isolamento & purificação , Acetil-CoA C-Acetiltransferase/metabolismo , Proteínas de Algas/genética , Proteínas de Algas/isolamento & purificação , Sequência de Aminoácidos , Animais , Enoil-CoA Hidratase/genética , Enoil-CoA Hidratase/isolamento & purificação , Enoil-CoA Hidratase/metabolismo , Euglena/ultraestrutura , Ácidos Graxos/metabolismo , Imuno-Histoquímica , Microscopia Imunoeletrônica , Mitocôndrias/enzimologia , Dados de Sequência Molecular , Peso Molecular , Complexos Multienzimáticos/genética , Complexos Multienzimáticos/isolamento & purificação , Racemases e Epimerases/genética , Racemases e Epimerases/isolamento & purificação , Racemases e Epimerases/metabolismo , Homologia de Sequência de Aminoácidos , Estereoisomerismo , Especificidade por Substrato
12.
J Ind Microbiol Biotechnol ; 28(2): 81-7, 2002 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-12074056

RESUMO

Cell free extracts of Galactomyces reessii contain a hydratase as the key enzyme for the transformation of 3-methylcrotonic acid to 3-hydroxy-3-methylbutyric acid. Highest levels of hydratase activity were obtained during growth on isovaleric acid. The enzyme, an enoyl CoA hydratase, was purified 147-fold by precipitation with ammonium sulphate and successive chromatography over columns of DE-52, Blue Sepharose CL-6B and Sephacryl S-200. During purification, hydratase activity was measured spectrophotometrically (OD change at 263 nm) for 3-methylcrotonyl CoA and crotonyl CoA as substrates. The enzyme displayed highest activity with crotonyl CoA with a Kcat of 1,050,000 min(-1). The ratio of crotonyl CoA to 3-methylcrotonyl CoA activities was constant (20:1) during all steps of purification. The Kcat for crotonyl CoA was also about 20 times greater than the Kcat for 3-methylcrotonyl CoA (51,700 min(-1). The enzyme had pH and temperature optima at 7.0 and 35 degrees C, a native Mr of 260 +/- 4.5 kDa and a subunit Mr of 65 kDa, suggesting that the enzyme was a homotetramer. The pI of the purified hydratase was 5.5, and the N-terminal amino acid sequence was VPEGYAEDLLKGKMMRFFDS. Hydratase activity for 3-methylcrotonyl CoA was competitively inhibited by acetyl CoA, propionyl CoA and acetoacetyl CoA.


Assuntos
Butiratos/metabolismo , Enoil-CoA Hidratase/isolamento & purificação , Saccharomycetales/metabolismo , Sequência de Aminoácidos , Catálise , Enoil-CoA Hidratase/química , Enoil-CoA Hidratase/metabolismo , Indução Enzimática , Dados de Sequência Molecular
13.
Appl Microbiol Biotechnol ; 53(2): 209-18, 2000 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-10709984

RESUMO

An (R)-trans-2,3-enoylacyl-CoA hydratase was purified to near-homogeneity from Rhodospirillum rubrum. Protein sequencing of enriched protein fractions allowed the construction of a degenerate oligonucleotide. The gene encoding the (R)-specific hydratase activity was cloned following three rounds of colony hybridization using the oligonucleotide, and overexpression of the gene in E. coli led to the purification of the enzyme to homogeneity. The purified enzyme used crotonyl-CoA, trans-2,3-pentenoyl-CoA, and trans-2,3-hexenoyl-CoA with approximately equal specificity as substrates in the hydration reaction. However, no activity was observed using trans-2,3-octenoyl-CoA as a substrate, but this compound did partially inhibit crotonyl-CoA hydration. Based on the nucleotide sequence, the protein has a monomeric molecular weight of 15.4 kDa and is a homotetramer in its native form as determined by gel filtration chromatography and native PAGE. The hydratase was expressed together with the PHA synthase from Thiocapsa pfennigii in E. coli strain DH5alpha. Growth of these strains on oleic acid resulted in the production of the terpolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) .


Assuntos
Enoil-CoA Hidratase/genética , Genes Bacterianos , Hidroxiácidos/metabolismo , Rhodospirillum rubrum/genética , Acil Coenzima A/metabolismo , Aciltransferases/metabolismo , Sequência de Aminoácidos , Clonagem Molecular , Enoil-CoA Hidratase/biossíntese , Enoil-CoA Hidratase/isolamento & purificação , Escherichia coli/metabolismo , Expressão Gênica , Cinética , Dados de Sequência Molecular , Ácido Oleico , Fases de Leitura Aberta , Proteínas Recombinantes/biossíntese , Rhodospirillum rubrum/enzimologia , Alinhamento de Sequência , Thiocapsa/enzimologia , Thiocapsa/genética
14.
J Biol Chem ; 273(47): 31366-74, 1998 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-9813046

RESUMO

We have identified the Saccharomyces cerevisiae gene ECI1 encoding Delta3-cis-Delta2-trans-enoyl-CoA isomerase that acts as an auxiliary enzyme in the beta-oxidation of (poly)unsaturated fatty acids. A mutant devoid of Eci1p was unable to grow on media containing unsaturated fatty acids such as oleic acid but was proficient for growth when a saturated fatty acid such as palmitic acid was the sole carbon source. Levels of ECI1 transcript were elevated in cells grown on oleic acid medium due to the presence in the ECI1 promoter of an oleate response element that bound the transcription factors Pip2p and Oaf1p. Eci1p was heterologously expressed in Escherichia coli and purified to homogeneity. It was found to be a hexameric protein with a subunit of molecular mass 32, 000 Da that converted 3-hexenoyl-CoA to trans-2-hexenoyl-CoA. Eci1p is the only known member of the hydratase/isomerase protein family with isomerase and/or 2-enoyl-CoA hydratase 1 activities that does not contain a conserved glutamate at its active site. Using a green fluorescent protein fusion, Eci1p was shown to be located in peroxisomes of wild-type yeast cells. Rat peroxisomal multifunctional enzyme type I containing Delta3-cis-Delta2-trans-enoyl-CoA isomerase activity was expressed in ECI1-deleted yeast cells, and this restored growth on oleic acid.


Assuntos
3-Hidroxiacil-CoA Desidrogenases/metabolismo , Enoil-CoA Hidratase/metabolismo , Ácidos Graxos Insaturados/metabolismo , Genes Fúngicos , Isomerases/metabolismo , Microcorpos/enzimologia , Complexos Multienzimáticos/metabolismo , Saccharomyces cerevisiae/genética , 3-Hidroxiacil-CoA Desidrogenases/deficiência , 3-Hidroxiacil-CoA Desidrogenases/genética , 3-Hidroxiacil-CoA Desidrogenases/isolamento & purificação , Sequência de Aminoácidos , Domínio Catalítico , Compartimento Celular , Sequência Conservada , Enoil-CoA Hidratase/deficiência , Enoil-CoA Hidratase/genética , Enoil-CoA Hidratase/isolamento & purificação , Indução Enzimática , Proteínas de Fluorescência Verde , Isomerases/deficiência , Isomerases/genética , Isomerases/isolamento & purificação , Isomerismo , Proteínas Luminescentes/genética , Proteínas Luminescentes/isolamento & purificação , Complexos Multienzimáticos/deficiência , Complexos Multienzimáticos/genética , Complexos Multienzimáticos/isolamento & purificação , Mutação , Ácido Oleico/metabolismo , Ácido Palmítico/metabolismo , Enzima Bifuncional do Peroxissomo , Regiões Promotoras Genéticas , Conformação Proteica , RNA Mensageiro/análise , Proteínas Recombinantes de Fusão/isolamento & purificação , Elementos de Resposta , Saccharomyces cerevisiae/enzimologia , Saccharomyces cerevisiae/crescimento & desenvolvimento , Homologia de Sequência de Aminoácidos
15.
Eur J Biochem ; 255(3): 618-27, 1998 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-9738901

RESUMO

Many aromatic compounds can be metabolized by bacteria under anoxic conditions via benzoyl-CoA as the common intermediate. The central pathway of benzoyl-CoA metabolism is initiated by an ATP-driven reduction of the aromatic ring producing cyclohexa-1,5-diene-1-carbonyl-CoA. The 1,5-dienoyl-CoA intermediate is thought to be transformed to 6-hydroxycyclohex-1-ene-1-carbonyl-CoA by a specific dienoyl-CoA hydratase catalyzing the formal addition of water to one of the double bonds. This dienoyl-CoA hydratase was detected in the denitrifying bacterium Thauera aromatica after anaerobic growth with benzoate. Substrate and product were confirmed and a convenient spectrophotometric assay was developed. The equilibrium concentrations of substrate and product were almost equal. Enzyme activity was induced after anoxic growth with benzoate, in contrast to acetate. The enzyme of 28 kDa was purified from T. aromatica and was found to be highly specific for the cyclic 1,5-dienoyl-CoA. A second 29-kDa enoyl-CoA hydratase acted on crotonyl-CoA; this highly active enoyl-CoA hydratase also acted slowly on cyclohex-1-ene-1-carbonyl-CoA. The regulation of expression of dienoyl-CoA hydratase activity, the kinetic constants, the substrate specificity, and the specific activity of the enzyme in cell extract provide evidence that dienoyl-CoA hydratase is the second enzyme of the central benzoyl-CoA pathway of anaerobic aromatic metabolism in T. aromatica. Extracts of Rhodopseudomonas palustris contained high activity of cyclohex-1-ene-1-carbonyl-CoA hydratase, but no 1,5-dienoyl-CoA hydratase activity. It appears that a variant of the benzoyl-CoA pathway is operating in R. palustris in which hydration of the 1,5-dienoyl-CoA does not take place. Rather, cyclohex-1-ene-1-carbonyl-CoA is hydrated to 2-hydroxycyclohexane-1-carbonyl-CoA [corrected].


Assuntos
Acil Coenzima A/metabolismo , Bactérias/metabolismo , Enoil-CoA Hidratase/metabolismo , Hidroliases/metabolismo , Sequência de Aminoácidos , Anaerobiose , Enoil-CoA Hidratase/isolamento & purificação , Hidroliases/isolamento & purificação , Dados de Sequência Molecular
16.
Proc Natl Acad Sci U S A ; 92(6): 2051-5, 1995 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-7892223

RESUMO

AU-rich elements within the 3' untranslated region of transcripts of lymphokines and some protooncogenes serve as signal for rapid mRNA degradation. By using an AUUUA matrix, we have affinity-purified a 32-kDa protein, microsequenced it, and cloned the corresponding cDNA. In vitro, the recombinant protein bound specifically to AU-rich transcripts, including those for interleukin 3, granulocyte/macrophage colony-stimulating factor, c-fos, and c-myc. Sequence analysis revealed an unexpected homology to enoyl-CoA hydratase (EC 4.2.1.17), and the recombinant protein showed a low degree of the enzymatic activity. Thus, this gene, designated AUH, encodes an RNA binding protein with intrinsic enzymatic activity. Protein immobilized on an AUUUA matrix was enzymatically active, suggesting that hydratase and AU-binding functions are located on distinct domains within a single polypeptide.


Assuntos
Encéfalo/metabolismo , Enoil-CoA Hidratase/biossíntese , Enoil-CoA Hidratase/metabolismo , Proteínas de Ligação a RNA/biossíntese , Proteínas de Ligação a RNA/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Western Blotting , Cromatografia de Afinidade , Clonagem Molecular , Primers do DNA , Enoil-CoA Hidratase/isolamento & purificação , Biblioteca Gênica , Genes fos , Genes myc , Fator Estimulador de Colônias de Granulócitos e Macrófagos/biossíntese , Humanos , Immunoblotting , Interleucina-3/biossíntese , Dados de Sequência Molecular , Oligodesoxirribonucleotídeos , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/isolamento & purificação , Peptídeos/química , Peptídeos/imunologia , Reação em Cadeia da Polimerase , Proteínas de Ligação a RNA/isolamento & purificação , Ratos , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Homologia de Sequência de Aminoácidos , Transcrição Gênica
17.
Eur J Biochem ; 228(1): 68-73, 1995 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-7883013

RESUMO

Mitochondrial 2-enoyl-CoA hydratase (mECH) and 3,2-trans-enoyl-CoA isomerase (mECI), two enzymes which catalyze totally different reactions in fatty acid beta-oxidation, belong to the low-similarity hydratase/isomerase enzyme superfamily. Their substrates and reaction mechanisms are similar [Müller-Newen, G. & Stoffel, W. (1993) Biochemistry 32, 11,405-11,412]. Glu164 of mECH is the only amino acid with a protic side chain that is conserved in these monofunctional and polyfunctional enzymes with 2-enoyl-CoA hydratase and 3,2-trans-enoyl-CoA isomerase activities. We tested our hypothesis that Glu164 of mECH is the putative active-site amino acid responsible for the base-catalyzed alpha-deprotonation in the hydratase/dehydrase and isomerase reaction. We functionally expressed rat liver mECH wild-type and [E164Q] mutant enzymes in Escherichia coli. Characterization of the purified wild-type and mutant enzymes revealed that the replacement of Glu164 by Gln lowers the kcat value more than 100,000-fold, whereas the Km value is only moderately affected. We have demonstrated in a previous study that Glu165 is indispensable for the 3,2-trans-enoyl-CoA isomerase activity. Taking these results together, we conclude that the conserved glutamic acid is the essential basic group in the active sites of 2-enoyl-CoA hydratase (Glu164) and 3,2-trans-enoyl-CoA isomerase (Glu165), and that these enzymes are not only evolutionarily but also functionally and mechanistically related.


Assuntos
Isomerases de Ligação Dupla Carbono-Carbono , Enoil-CoA Hidratase/metabolismo , Ácido Glutâmico/metabolismo , Isomerases/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Sítios de Ligação , Dodecenoil-CoA Isomerase , Enoil-CoA Hidratase/química , Enoil-CoA Hidratase/isolamento & purificação , Escherichia coli/genética , Isomerases/química , Isomerases/isolamento & purificação , Dados de Sequência Molecular , Ratos , Proteínas Recombinantes/metabolismo , Relação Estrutura-Atividade
18.
Comp Biochem Physiol B Biochem Mol Biol ; 109(4): 557-66, 1994 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-7881821

RESUMO

The subunit locations of the component enzymes of the pig heart trifunctional mitochondrial beta-oxidation complex are suggested by analyzing the primary structure of the large subunit of this membrane-bound multienzyme complex [Yang S.-Y. et al. (1994) Biochem. biophys. Res. Commun. 198, 431-437] with those of the subunits of the E. coli fatty acid oxidation complex and the corresponding mitochondrial matrix beta-oxidation enzymes. Long-chain enoyl-CoA hydratase and long-chain 3-hydroxyacyl-CoA dehydrogenase are located in the amino-terminal and the central regions of the 79 kDa polypeptide, respectively, whereas the long-chain 3-ketoacyl-CoA thiolase is associated with the 46 kDa subunit of this complex. The pig heart mitochondrial bifunctional beta-oxidation enzyme is more homologous to the large subunit of the prokaryotic fatty acid oxidation complex than to the peroxisomal trifunctional beta-oxidation enzyme. The evolutionary trees of 3-hydroxyacyl-CoA dehydrogenases and enoyl-CoA hydratases suggest that the mitochondrial inner membrane-bound bifunctional beta-oxidation enzyme and the corresponding matrix monofunctional beta-oxidation enzymes are more remotely related to each other than to their corresponding prokaryotic enzymes, and that the genes of E. coli multifunctional fatty acid oxidation protein and pig heart mitochondrial bifunctional beta-oxidation enzyme diverged after the appearance of eukaryotic cells.


Assuntos
3-Hidroxiacil-CoA Desidrogenases/isolamento & purificação , Enoil-CoA Hidratase/isolamento & purificação , Mitocôndrias Cardíacas/enzimologia , Complexos Multienzimáticos/química , 3-Hidroxiacil-CoA Desidrogenases/metabolismo , Sequência de Aminoácidos , Animais , Membrana Celular , Enoil-CoA Hidratase/metabolismo , Dados de Sequência Molecular , Complexos Multienzimáticos/metabolismo , Filogenia , Alinhamento de Sequência , Suínos
19.
Comp Biochem Physiol Biochem Mol Biol ; 108(4): 471-80, 1994 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-7953067

RESUMO

A simple and rapid purification procedure for hepatic peroxisomal multifunctional enzyme (delta 3, delta 2-enoyl-CoA isomerase/enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase) from clofibrate treated mice is described. The purification is achieved within two days using ion-exchange chromatography and an easily prepared affinity resin. The overall yield is 10% or more after a 100-fold enrichment from the cytosolic fraction of liver tissue. The native enzyme is a monomer with a molecular mass of 75 kDa. The protein is blocked in the N-terminus but internal amino acid sequences was obtained after proteolytic cleavage. Western blot analysis indicated proteolysis of multifunctional enzyme in different subcellular fractions derived from liver tissue. The hydratase activity of the enzyme is heat-labile and highly dependent on the concentration of Tris buffer or potassium chloride present. Optimal activity was found around 37 degrees C and pH 7. The enzyme also shows dehydrogenase and isomerase activity.


Assuntos
3-Hidroxiacil-CoA Desidrogenases/isolamento & purificação , Enoil-CoA Hidratase/isolamento & purificação , Isomerases/isolamento & purificação , Fígado/enzimologia , Complexos Multienzimáticos/isolamento & purificação , 3-Hidroxiacil-CoA Desidrogenases/química , Sequência de Aminoácidos , Animais , Western Blotting , Cromatografia por Troca Iônica , Eletroforese em Gel de Poliacrilamida , Enoil-CoA Hidratase/química , Isomerases/química , Masculino , Camundongos , Camundongos Endogâmicos , Microcorpos/enzimologia , Dados de Sequência Molecular , Peso Molecular , Complexos Multienzimáticos/química , Enzima Bifuncional do Peroxissomo
20.
Toxicol Lett ; 71(3): 271-7, 1994 May.
Artigo em Inglês | MEDLINE | ID: mdl-8160215

RESUMO

The effect of in vivo exposure to perfluoro-n-octanoic and perfluoro-n-decanoic acids was examined in the rat liver by two-dimensional electrophoresis (2DE). Using nonequilibrium pH-gradient electrophoresis in the first dimension separation, proteins associated with the mitochondrial/peroxisomal cell fraction were observed and immunologically identified. Conspicuous inductions in peroxisomal enoyl-CoA hydratase and other proteins of the peroxisomal beta-oxidative pathway were observed following single-dose exposure to each compound. The abundance of the tentatively-identified mitochondrial equivalent, crotonase, was not altered by these intoxications. These results confirm previous observations of perfluorocarboxylic acid toxicity and support the use of 2D protein-pattern alterations in biomarker research. The ability to identify this type of alteration via 2DE, in association with specific toxic effects by chemically related compounds, may provide new and additional markers for chemical-induced tissue damage.


Assuntos
Caprilatos/toxicidade , Ácidos Decanoicos/toxicidade , Enoil-CoA Hidratase/biossíntese , Fluorocarbonos/toxicidade , Fígado/enzimologia , Animais , Caprilatos/administração & dosagem , Ácidos Decanoicos/administração & dosagem , Eletroforese em Gel Bidimensional , Enoil-CoA Hidratase/efeitos dos fármacos , Enoil-CoA Hidratase/isolamento & purificação , Indução Enzimática/efeitos dos fármacos , Fluorocarbonos/administração & dosagem , Dose Letal Mediana , Fígado/efeitos dos fármacos , Masculino , Ratos , Ratos Endogâmicos F344
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