Regional and Long-Term Analyses of Stable Isotopes of Fish and Invertebrates Show Evidence of the Closure of a Pulp Mill and the Influence of Additional Stressors.

A bleached kraft pulp mill discharging effluent to the Mattagami River in northern Ontario, Canada, closed after almost 90 yr of operation. During its operation, effluent from the mill influenced biota in the downstream areas. To assess shifts in the reliance of biota from mill-derived nutrients, the isotopic composition (δ13 C and δ15 N) of white sucker (Catostomus commersoni) muscle and whole mayflies (Hexagenia sp.) were compared before (1990s) and after the pulp mill's closure (2012-2014). To better understand other potential sources of spatial and temporal change, samples from 3 other tributaries in the basin with dams, ongoing pulp mill operations, sites receiving sewage, and at several reference sites were collected and compared. Irrespective of time period, biota collected at sites downstream of both dams and active pulp mills tended to have elevated δ13 C values, but variable changes in δ15 N (negligible in most cases) when compared with upstream samples. The isotopic composition of mayflies varied at reference sites over time, with decreasing values of δ13 C and δ15 N (mayflies only) with increasing depth, and there was evidence of lower δ13 C in fish after the pulp mill closure. Overall, these results suggest the importance of long-term, regional-scale measurements for documenting the effects of stressors on nutrient use by aquatic species. Environ Toxicol Chem 2020;39:1207-1218. © 2020 SETAC.

Assessment of heavy metals in benthic macroinvertebrates, water and sediments in River Isiukhu, Kenya.

A major ecological challenge facing freshwater resources such as rivers is the influx of chemical contaminants from anthropogenic sources. A report on the levels of heavy metals, namely Zn, Cu, Cr, Cd and Pb in benthic macroinvertebrates, sediments and water along River Isiukhu, within Lake Victoria catchment area in Kenya is presented. Families of benthic macroinvertebrates that were investigated included Gerridae, Baetidae and Unionidae. Samples were digested using the appropriate acid combination and analysed using Spectra AAS Varian 200. Physicochemical parameters were measured on-site using mobile Hydrolab Quanta. Pearson's correlation matrix and post hoc Tukey's test were used to show the relationship between metal concentrations in the various matrices at significant differences accepted at p ≤ 0.05. Positive correlations were obtained for heavy metal concentrations in sediments and benthic macroinvertebrates with significant correlations observed for Zn (r = 0.655, p = 0.029) and Cu (r = 0.641, p = 0.034). Sediments presented the highest range of heavy metal concentrations compared to water and benthic macroinvertebrates and presented the only matrix with quantifiable Pb levels. The range in heavy metal concentration in benthic macroinvertebrates samples were as follows: Zn, 30.73-46.64, 21.93-38.17, and 26.85-41.69 mg/kg, Cu, 1.17-6.54, 1.11-3.87 and 1.15-5.79 mg/kg, Cr, 0.47-1.61, 0.22-0.74 and 0.25-0.92 mg/kg, for families Unionidae, Baetidae and Gerridae respectively. Heavy metal concentration profile along the river indicated an influx of pollutants from anthropogenic sources due to rapid urbanization along the river.

Antitumor activity of chitosan from mayfly with comparison to commercially available low, medium and high molecular weight chitosans.

Insects' cuticles have a potential to be evaluated as a chitin source. Especially adults of aquatic insects like mayflies (order Ephemeroptera) swarm in enormous numbers in artificially lit areas while mating in spring and then die by leaving huge amounts of dead insects' bodies. Here in this study, mayfly corpses were harvested and used for production of low MW chitosan. Dried mayfly bodies had 10.21% chitin content; mayfly chitin was converted into chitosan with efficiency rate of 78.43% (deacetylation degree, 84.3%; MW, 3.69 kDa). Cytotoxicity and anti-proliferative activity of mayfly and commercially available shrimp chitosans (low, medium, and high MW) were determined on L929 fibroblast and three different cancer types including HeLa, A549, and WiDr. Apoptosis and necrosis stimulating potential of mayfly and commercial chitosans were also evaluated on A549 and WiDr cells using acridine orange and propidium iodide dual staining to observe morphological changes in nuclei and thus to reveal the predominant cell death mechanism. The effects of chitosans have varied depending on cell types, concentration, and chitosan derivatives. Mayfly and low MW chitosans had a cytotoxic effect at a concentration of 500 µg mL-1 on non-cancer cells. At concentrations below this value (250 µg mL-1), mayfly and commercial chitosans except high MW one exhibited strong inhibitory activity on cancer cells especially A549 and WiDr cells. Mayfly chitosan induced early and late apoptosis in A549 cells, but late apoptosis and necrosis in WiDr cells. This study suggests that dead bodies of mayflies can be used for production of low MW chitosan with anti-proliferative activity.

Arsenic (V) bioconcentration kinetics in freshwater macroinvertebrates and periphyton is influenced by pH.

Arsenic is an important environmental pollutant whose speciation and mobility in freshwater food webs is complex. Few studies have characterized uptake and efflux rates of arsenic in aquatic benthic invertebrates. Further, we lack a fundamental understanding of how pH influences uptake kinetics in these organisms or how this key environmental variable could alter dietary exposure for primary consumers. Here we used a radiotracer approach to characterize arsenate accumulation dynamics in benthic invertebrates, the influence of pH on uptake in a subset of these organisms, and the influence of pH on uptake of arsenate by periphyton - an important food source at the base of aquatic food webs. Uptake rate constants (Ku) from aqueous exposure were modest, ranging from ∼0.001 L g-1d-1 in three species of mayfly to 0.06 L g-1d-1in Psephenus herricki. Efflux rate constants ranged from ∼0.03 d-1 in Corbicula fluminea to ∼0.3 d-1 in the mayfly Isonychia sp, and were generally high. Arsenate uptake decreased with increasing pH, which may be a function of increased adsorption at lower pHs. A similar but much stronger correlation was observed for periphyton where Ku decreased from ∼3.0 L g-1d-1 at 6.5 pH to ∼0.7 L g-1d-1 at 8.5 pH, suggesting that site specific pH could significantly alter arsenic exposure, particularly for primary consumers. Together, these findings shed light on the complexity of arsenic bioavailability and help explain observed differences reported in the literature.

Polychlorinated Biphenyl Bioaccumulation Patterns Among Lake Erie Lower Trophic Level Consumers Reflect Species Ecologies.

Polychlorinated biphenyl (PCB) concentrations were quantified in lower trophic level consumers of the Lake Erie western basin food web to assess the capacity of PCBs to discriminate among trophically similar species inhabiting different compartments of the same ecosystem. Zooplankton were characterized by higher proportions of less chlorinated and hydrophobic homologs relative to zebra mussel (Dreissena polymorpha), mayfly (Hexagenia limbata) and emerald shiner (Notropis atherinoides) samples. PCB biota-sediment accumulation factors (BSAF) differed significantly among species with zebra mussels and emerald shiners having the highest BSAFs. Principal components analysis of sample PCB profiles reflected the contrasting pelagic and benthic habitats occupied by filter-feeding zooplankton and zebra mussel samples. Benthic mayfly PCB profiles were characterized by increasingly hydrophobic (logK OW ≥ 6.9) congeners with more variable emerald shiner profiles reflecting the greater mobility and extent of spatial habitat integration achieved by this secondary consumer. These results contribute to growing evidence for the use of PCBs as ecological tracers in aquatic ecosystems.

A comparison of freeze-drying and oven-drying preparation methods for bulk and compound-specific carbon stable isotope analyses: examples using the benthic macroinvertebrates Stenopsyche marmorata and Epeorus latifolium.

RATIONALE: Carbon stable isotope analysis of bulk samples and fatty acids is an established method for tracing carbon flow pathways and reconstructing trophic interactions, but there is no consensus on which sample drying method should be used for sample preparation. The aim of this study was to determine if freeze-drying and oven-drying treatments used to prepare samples of the benthic macroinvertebrates Stenopsyche marmorata and Epeorus latifolium for bulk and fatty-acid-specific carbon stable isotope analysis yield different isotopic ratio values. METHODS: Five individuals each from two species were split in half; one half was freeze-dried and the other half was oven-dried. The samples were ground and the δ(13)C values of the bulk samples and eight fatty acids were measured following combustion using an isotope ratio mass spectrometer coupled to an elemental analyzer or gas chromatography system. RESULTS: The mean difference in the bulk and fatty acid δ(13)C values between freeze-dried and oven-dried samples was small (≤0.1‰ in both cases), although relatively large variations were observed in individual fatty-acid-specific δ(13)C values (maximum of ≤0.9 ‰). There were no significant differences in either bulk sample or fatty-acid-specific δ(13)C values between freeze-dried or oven-dried samples of the same species. CONCLUSIONS: Freeze-drying and oven-drying are equally acceptable methods for preparing freshly caught S. marmorata and E. latifolium samples for bulk and fatty-acid-specific carbon stable isotope analyses.

Elevated major ion concentrations inhibit larval mayfly growth and development.

Anthropogenic disturbances, including those from developing energy resources, can alter stream chemistry significantly by elevating total dissolved solids. Field studies have indicated that mayflies (Order Ephemeroptera) are particularly sensitive to high total dissolved solids. In the present study, the authors measured 20-d growth and survivorship of larval Neocloeon triangulifer exposed to a gradient of brine salt (mixed NaCl and CaCl2 ) concentrations. Daily growth rates were reduced significantly in all salt concentrations above the control (363 µS cm(-1) ) and larvae in treatments with specific conductance >812 µS cm(-1) were in comparatively earlier developmental stages (instars) at the end of the experiment. Survivorship declined significantly when specific conductance was >1513 µS cm(-1) and the calculated 20-d 50% lethal concentration was 2866 µS cm(-1) . The present study's results provide strong experimental evidence that elevated ion concentrations similar to those observed in developing energy resources, such as oil and gas drilling or coal mining, can adversely affect sensitive aquatic insect species.

Effects of coal fly ash on tree swallow reproduction in Watts Bar Reservoir, Tennessee.

Coal-fly ash was released in unprecedented amounts (4.1 × 10(6) m(3) ) into the Emory River from the Tennessee Valley Authority Kingston Fossil Plant on Watts Bar Reservoir in Tennessee. Tree swallows were exposed to ash-related constituents at the ash release via their diet of emergent aquatic insects, whose larval forms can accumulate constituents from submerged river sediments. Reproduction of tree swallow colonies was assessed over a 2-year period by evaluating whether 1) ash constituent concentrations were elevated in egg, eggshell, and nestling tissues at colonies near ash-impacted river reaches compared to reference colonies, 2) production of fledglings per nesting female was significantly lower in ash-impacted colonies versus reference colonies, and 3) ash constituent concentrations or diet concentrations were correlated with nest productivity measures (clutch size, hatching success, and nestling survival, and fledglings produced per nest). Of the 26 ash constituents evaluated, 4 (Se, Sr, Cu, and Hg) were significantly elevated in tissues potentially from the ash, and 3 (Se, Sr, and Cu) in tissues or in swallow diet items were weakly correlated to at least one nest-productivity measure or egg weight. Tree swallow hatching success was significantly reduced by 12%, but fledgling production per nest was unaffected due to larger clutch sizes in the impacted than reference colonies. Bioconcentration from the ash to insects in the diet to tree swallow eggs appears to be low. Overall, adverse impacts of the ash on tree swallow reproduction were not observed, but monitoring is continuing to further ensure Se from the residual ash does not adversely affect tree swallow reproduction over time. Integr Environ Assess Manag 2015;11:56-66. © 2014 SETAC.

Histological effects and localization of dissolved microcystins LR and LW in the mayfly Ecdyonurus angelieri Thomas (Insecta, Ephemeroptera).

The ability of microcystins (MCs), the main group of cyanotoxins, to affect the physiological processes and tissues of insects has received little attention. Fresh water dissolved MCs represent one of the main sources of cyanotoxins. In the experiment described herein, captured wild mayfly Ecdyonurus angelieri Thomas, 1968 larvae were exposed to 5 ppb of two distinct microcystins, MC-LR and MC-LW, in separate assays. Evidence of induced mortality, MCs bioaccumulation and severe histological damage affecting fat body and alterations in the tracheal system were evident. Our results reveal the acute sensitivity of the mayfly E. angelieri to MCS, which may serve as early indicators or cyanotoxins production and the quality of freshwater streams.