Effects of UV-C treatment and cold storage on ergosterol and vitamin D2 contents in different parts of white and brown mushroom (Agaricus bisporus).

Effects of ultraviolet-C (UV-C) treatment (0.5, 1.0 and 2.0kJ/m(2)) and cold storage on ergosterol and vitamin D2 content in different parts of white and brown button mushrooms (Agaricus bisporus) were investigated. UV-C treatment did not significantly affect ergosterol content in the caps and stems of the two mushrooms, but ergosterol content increased significantly during 14days cold storage. Vitamin D2 content in the caps and stems of two mushrooms significantly increased as UV-C dose increased, and 2.0kJ/m(2) UV-C showed the best result. During cold storage, vitamin D2 content in the caps of the two mushrooms decreased from day 1 to day 7, and then kept stable until day 14, but vitamin D2 content in the stems of brown mushrooms kept increasing for the whole 14days period. UV-C could increase vitamin D2 contents in both caps and stems of white and brown mushrooms without significantly affecting ergosterol content.

Voluntary exposure of some western-hemisphere snake and lizard species to ultraviolet-B radiation in the field: how much ultraviolet-B should a lizard or snake receive in captivity?

Studies of voluntary exposure to ultraviolet-B (UVB) radiation from the sun in the field were conducted in the southern US and Jamaica for 15 species of lizards and snakes occupying various habitats. Species were sorted into four zones of UVB exposure ranging from a median UV index of 0.35 for zone 1 to 3.1 for zone 4. Guidelines for UVB exposure in captivity of these and species occupying similar light environments are presented. Data for most species were collected during mid-day during the spring breeding season, which appeared to be the time of maximum exposure. For two species of Sceloporus studied more intensively there was significant variation of exposure among times of the day and among seasons. So, all-day studies over the entire active season are necessary to fully understand the pattern of natural exposure for a particular diurnal species. Environmental and body temperature and thermoregulation as well as UVB/vitamin D photoregulation influences exposure to UVB. Regressions allowing the inter-conversion of readings among some meters with different detector sensitivities are presented. Readings of natural sunlight predict the same photobiosynthetic potential for vitamin D as the same reading from artificial sources whose wavelength distribution within the UVB band of the source is comparable to that of sunlight. Research approaches to further increase our understanding of vitamin D and UVB use and requirements for squamate reptiles in captivity are outlined.

A new look into the reaction between ergosterol and singlet oxygen in vitro.

The reaction of ergosterol (ERGO) with singlet oxygen in vitro was studied by using different combinations of the photosensitizers (i.e. rose Bengal and eosine) and solvents (i.e. pyridine, ethanol and methyl tert-butyl ether) and all the products obtained were isolated and fully characterized (mp, Rf, UV, 1H-NMR, 13C-NMR, EI-MS, ESI-MS and HR-MS). In pyridine. together with the expected (22E)-5alpha,8alpha-epidioxyergosta-6,22-dien-3beta-ol, EEP, the keto derivative (22E)-3beta-hydroxyergosta-5,8(9),22-trien-7-one. KE, was obtained. In ethanol the expected EEP was obtained together with (22E)-5alpha,8alpha-epidioxyergosta-6,9,22-trien-3beta-ol, EEP9(11), and (22E)-ergosta-6,9,22-triene-3beta,5alpha,8alpha-triol, DHOE, as main products and (22E)-ergosta-5,7,9,22-tetraen-3beta-ol, DHE, in trace amounts In methyl tert-butyl ether, a complex mixture of EEP, KE, DHOE, EEP9(11), DHE, together with (22E)-7alpha-hydroperoxyergosta-5.8(9),22-trien-3beta-ol, EHP, and (22E)-ergosta-5,8(9),22-triene-3beta, 7alpha-diol, EH, was obtained. The minor products were characterized and showed strong dependence on the reaction medium. The regioselective and stereoselective character of the singlet oxygen attack on the ERGO diene moiety is discussed in terms of ERGO HOMO's properties.

Photochemical reactions and phototoxicity of sterols: novel self-perpetuating mechanisms for lipid photooxidation.

Sterols are important lipid components that may contribute to phototoxicity. We have found that phototoxic response in earthworms is related to sterols extractable with lipophilic solvents. The photochemically active compounds in worm lipids are 5,7,9(11),22-ergostatetraen-3 beta-ol (9-DHE) and 5,7,9(11)-cholestartien-3 beta-ol (9-DDHC), respectively. Human skin lipids are known to contain 9-DHE. We have also found 9-DDHC in human skin, which is reported here for the first time. In the presence of an excess of the corresponding 5,7-dienes (ergosterol of 7-dehydrocholesterol), these photoactive sterols constitute a self-regenerating source of singlet molecular oxygen (1O2) during irradiation in vivo or in vitro with UVA (315-400 nm). The quantum yield for photosensitization of 1O2 by 9-DHE was estimated to be 0.09. The 1O2 is scavenged by the dienes and the rate constant for 1O2 quenching by ergosterol was found to be 1.2 x 10(7) M-1 s-1 in methyl t-butyl ether (MTBE). This scavenging ultimately leads to the production of 5,8-endoperoxide and hydrogen peroxide. Photochemically induced superoxide radical was also produced on irradiation of sterol 5,7,9-trienes and trapped with the spin trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO). The production of singlet oxygen, peroxides and radicals by the sterols may be significant in the cell damaging and tumor promoting action of UVA light on skin.

Near-UV-induced absorbance change and photochemical decomposition of ergosterol in the plasma membrane of the yeast Saccharomyces cerevisiae.

When cells of the yeast Saccharomyces cerevisiae were exposed to near-UV (300-400 nm), their absorption spectra changed slightly within the range 220-300 nm with increasing dosage. Difference spectra, calculated by substracting the curve recorded in cells exposed to near-UV from the curve of unexposed cells, decreased with increasing dosage over a broad band with peaks at 272, 282 and 295 nm and a shoulder at 265 nm. These peaks were in agreement with the absorption maxima of ergosterol, which is one of the major components of the plasma membrane of yeast. Near-UV radiation induced a simultaneous decrease in absorption spectra and reduction of ergosterol content in the plasma membrane. Photochemical decomposition of ergosterol by near-UV radiation was revealed in vivo, although ergosterol is generally known to be photoconverted to previtamin D2 industrially by UV radiation in vitro. In order to remove photosensitizers, liposomes were prepared from phospholipids and glycolipids, with or without ergosterol from purified yeast plasma membranes. Liposomal ergosterol in the orientated state was photochemically decomposed by near-UV radiation but ergosterol in the disorientated state in a homogeneous solution was not. Near-UV radiation also induced a decrease in activity of membrane-bound ATPase. Dose-response curves for the reduction of ATPase activity were similar to that for decomposition of ergosterol, suggesting that near-UV caused membrane function damage.

[Characteristics of the final stages of ergosterol biosynthesis in Saccharomyces cerevisiae yeasts]. / Nekotorye osobennosti konechnykh stadii biosinteza érgosterina u drozhzhei Saccharomyces cerevisiae.

A scheme of ergosterol cyclic intermediate transformations in S. cerevisiae has been proposed. It is based on the analysis of sterol composition in strains with mutant blocks of one or two reactions of enzymatic synthesis. Biochemical reactions of modification of cyclic part and side branch of sterol molecule are supposed to be carried out independently.

Simultaneous determination of eight vitamin D2 isomers by proton magnetic resonance spectroscopy.

Simultaneous determination of vitamin D2 and its isomers, with the aid of pmr employing a paramagnetic shift reagent Eu (dpm)3, is presented. Thus, a mixture of vitamin D2 isomers is acetylated and its pmr spectra are run with and without the addition of the shift reagent. Relative paramagnetic shift values (gamma rel) of the acetoxyl protons of the acetylated isomers are estimated for solutions in CCl4 against the reference acetoxyl signal of vitamin D2 acetate. Estimated values are as follows: vitamin D2 acetate = 100, 5,6-trans-vitamin D2 acetate = 71 +/- 1, ergosteryl acetate = 86 +/- 1, lumisteryl2 acetate = 123 +/- 3, isovitamin D2 acetate = 82, isotachysteryl2 acetate = 97, tachysteryl2 acetate = 100 (105 +/- 1 in C6D6), and previtamin D2 acetate = 80 +/- 2. On the basis of a dramatic shift of an acetoxyl signal and a constancy of each gamma rel, this method permits immediate identification of the isomers and provides valuable information on their stereochemistry. This technique also facilitates a simultaneous determination of these isomers in a mixture by estimating each signal area.

[Effect of ultraviolet light on sterol-containing membranes in nystatin solutions]. / Deistvie ul'trafioletovogo sveta na membrany, soderzhashchie steriny, v rastovorakh nistatina

The nystatin induced conductivity of artificial phospholipid membranes containing ergosterol or 7-dehydrocholesterol drops sharply under the action of ultra-violet light as a result of the transformation of these provitamins into adequate D2 and D3 vitamins. The UV-light does not change the conductivity of the membranes containing cholestrol in nystatin solution. Nystatin slightly effects the membrane resistance in the presence of D2 and D3 vitamins.

[Photoisomerization of provitamins D under influence of various ultraviolet radiation]. / Fotoizomerizatsiia provitaminov D pod deistviem ul'trafioletovogo izlucheniia razlichnykh istochnikov

The influence of various sources of ultraviolet radiation on the photoisomerization of provitamins D--ergosterol and 7-dehydrocholesterol--was studied under industry-like conditions. The yield of previtamins and, consequently, vitamins D was higher with the use of erythemic lamps with luminophore E-2 and luminophore E-3 than with the use of lamps PRK-2.