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1.
Front Immunol ; 10: 581, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30972073

RESUMO

Triterpenes possess anti-inflammatory and anti-nociceptive effects. In this study anti-inflammatory activities of Asparacosin A were evaluated' using in-vitro cyclooxygenases 1 and 2 (COX-1/2) inhibition assays. Moreover, anti-nociceptive activities were assessed in-vivo by carrageenan-induced paw edema test, xylene-induced ear edema tests, and acetic acid-induced writhing and formalin tests. Additionally molecular docking was conducted to elucidate the binding mechanism of the compound and to correlate the in-vitro findings with the in-silico data. Oral administration of Asparacosin A at the doses of 10, 20, and 40 mg/kg induced significant anti-inflammatory effects (*p < 0.05, **p < 0.01, and ***p < 0.001) in a dose-dependent manner in both models. Asparacosin A also inhibited the human recombinant COX-2 enzyme and caused a dose-dependent decrease in the levels of TNF-α, IL-1ß, and PGE2 in the carrageenan-induced paws. Moreover, Asparacosin A displayed significant anti-nociceptive effects (*p < 0.05, **p < 0.01, ***p < 0.001) at the doses of 10, 20, and 40 mg/kg in acetic-acid induced writhing test. However, in formalin test, Asparacosin A (10-40 mg/kg, p.o) produced anti-nociceptive effects only in the late phase, similar to the effect observed with the reference drug celecoxib (50 mg/kg, p.o). Molecular docking was carried out on both COX-1 and COX-2 structures which revealed that Asparacosin A targets allosteric binding site similar to the binding mode of the selective COX inhibitor. In conclusion, Asparacosin A exhibits anti-inflammatory and peripheral anti-nociceptive activities which are likely mediated via inhibition of COX-2 enzyme and inflammatory cytokines. Furthermore, Asparacosin A can serve as a model to obtain new and more selective potent anti-inflammatory and anti-nociceptive drugs.


Assuntos
Analgésicos/farmacologia , Anti-Inflamatórios/farmacologia , Inibidores de Ciclo-Oxigenase 2/farmacologia , Citocinas/antagonistas & inibidores , Espirostanos/farmacologia , Animais , Ciclo-Oxigenase 2/química , Camundongos , Simulação de Acoplamento Molecular , Ratos , Ratos Sprague-Dawley , Espirostanos/toxicidade
2.
J Ethnopharmacol ; 154(2): 391-9, 2014 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-24747029

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: 'SHENMAI' injection (SMI) has been widely used in cardioprotection and modulation of the immune system because of its great efficacy. SMI primarily comprises the saponins from Panax ginseng and Ophiopogon japonicas. The profiles of saponins in SMI during long-term toxicokinetics remain unclear. MiR-146a possesses excellent sensitivity as a bio-marker in the innate immunity modification effect of SMI. AIM OF THE STUDY: Is to monitor the exposure level of SMI during a one-month toxicokinetic experiment, an analytical method involving ESI-LC-MS/MS technology was developed to determine 20 (S)-protopanaxadiol-type ginsenoside (Rb1, Rb2, Rc, Rd), 20 (S)-protopanaxatriol-type ginsenoside (Rg1, Re, Rf), oleanolic acid-type ginsenoside (Ro), and ophiopogonin D in rats. The levels of AST, CK, ALT, SOD, GSH-pX, MDA, miR-146a, and ECG were measured to explore the effects of SMI in cardiologic function and immune activity. RESULTS: Results show that the levels of AST, CK, and MDA decreased upon the administration of SMI. The level of miR-146a increased upon the administration of SMI dosage. During the administration of SMI, increasing exposure levels of 20 (S)-protopanaxadiol-type ginsenosides were also observed. CONCLUSION: The 20 (S)-protopanaxadiol-type ginsenosides were considered potential PK/TK markers because of their high exposure levels that continuously increased. Oxidative stress was slightly alleviated during the toxicokinetic study. Based on the level of miR-146a, negatively regulated innate immunity was observed. The regulation became more serious with increasing exposure levels of 20 (S)-protopanaxadiol-type ginsenosides. Negatively regulated innate immunity could be induced by long-term administration of SMI (>0.4g/kg).


Assuntos
Medicamentos de Ervas Chinesas/toxicidade , Ginsenosídeos/toxicidade , Imunidade Inata/efeitos dos fármacos , Saponinas/toxicidade , Espirostanos/toxicidade , Alanina Transaminase/sangue , Animais , Aspartato Aminotransferases/sangue , Biomarcadores/sangue , Creatina Quinase/sangue , Relação Dose-Resposta a Droga , Combinação de Medicamentos , Medicamentos de Ervas Chinesas/administração & dosagem , Medicamentos de Ervas Chinesas/farmacocinética , Etnofarmacologia , Feminino , Ginsenosídeos/administração & dosagem , Ginsenosídeos/sangue , Imunidade Inata/imunologia , Masculino , Medicina Tradicional Chinesa , MicroRNAs/sangue , Ratos Sprague-Dawley , Saponinas/administração & dosagem , Saponinas/sangue , Espirostanos/administração & dosagem , Espirostanos/sangue , Fatores de Tempo , Toxicocinética
3.
Bioorg Med Chem Lett ; 23(9): 2738-42, 2013 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-23511021

RESUMO

Bioassay-guided fractionation of the CHCl3 layer of Solanum violaceum areal parts methanolic extract led to the isolation of four new steroidal sapogenins, indiosides L-O (1-4), along with eight known steroids, one lignin, and a coumarin. Indioside L is a rare spirostanoid possessing a 1,4-dien-3-one moiety in ring A. Moreover, compounds 3 and 4 represent rare examples of spirostene with the 3ß,7α-diol-5,6-ene moiety compared to the normal 3ß,7ß-diol-5,6-ene derivatives. The cytotoxic activity of the isolates (5-14) was evaluated against human hepatoma (HepG2 and Hep3B), human lung carcinoma (A549), and human breast carcinoma (MDA-MB-231 and MCF-7).


Assuntos
Antineoplásicos/química , Solanum/química , Espirostanos/química , Antineoplásicos/isolamento & purificação , Antineoplásicos/toxicidade , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais , Células Hep G2 , Humanos , Células MCF-7 , Espectroscopia de Ressonância Magnética , Conformação Molecular , Folhas de Planta/química , Caules de Planta/química , Espirostanos/isolamento & purificação , Espirostanos/toxicidade
4.
Bioorg Med Chem Lett ; 23(6): 1771-5, 2013 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-23411079

RESUMO

Hosta longipes (FR. et SAV.) MATSUMURA (Liliaceae) is an edible vegetable in Korea. This study was conducted with the aim of evaluating the potential of H. longipes as a functional food for the treatment of neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. In this respect, the study resulted in the identification of three new steroidal compounds, longipenane (1), longipenane 26-O-ß-d-glucopyranoside (2) and neogitogenin 3-O-α-l-rhamnopyranosyl-(1→2)-O-[ß-d-glucopyranosyl-(1→4)]-ß-d-galactopyranoside (3), along with two known steroidal saponins (4 and 5). The identification and structural elucidation of these compounds were based on 1D and 2D NMR measurements, high-resolution FAB mass spectroscopy (HR-FAB-MS), and chemical methods. A proinflammatory mediator, nitric oxide (NO), in murine microglial BV-2 cells was used to assess the anti-neuroinflammatory effect of the isolated compounds from H. longipes. Among them, compounds 4 and 5 showed strong inhibitory effects on NO production without high cell toxicity in lipopolysaccharide-activated BV-2 cells (IC50=17.66 and 13.16µM, respectively).


Assuntos
Hosta/química , Óxido Nítrico/metabolismo , Saponinas/química , Espirostanos/química , Esteroides/química , Animais , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Espectroscopia de Ressonância Magnética , Camundongos , Microglia/efeitos dos fármacos , Conformação Molecular , Folhas de Planta/química , Saponinas/isolamento & purificação , Saponinas/toxicidade , Espirostanos/isolamento & purificação , Espirostanos/toxicidade , Esteroides/isolamento & purificação , Esteroides/toxicidade
5.
Mol Med Rep ; 7(3): 1038-44, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23314115

RESUMO

Deltonin, a steroidal saponin isolated from Dioscorea zingiberensis Wright, exhibits high cytotoxic activity in cancer cells. In the present study, the effects of deltonin on cell proliferation and apoptosis were evaluated in the MDA­MB­231 human breast carcinoma cell line. Following treatment with deltonin, the viability of MDA­MB­231 cells was analyzed using MTT assay and apoptosis, mitochondrial membrane potential (∆Ψm) alternation and intracellular reactive oxygen species (ROS) generation was determined by flow cytometry. In addition, western blot analysis was performed to examine the expression of apoptosis­associated proteins. The results demonstrated that deltonin induced apoptosis in MDA­MB­231 cells in a time­ and concentration­dependent manner. Apoptosis was associated with depolarization of ∆Ψm and time­dependent ROS generation. Deltonin treatment also resulted in Bax upregulation, Bcl-2 downregulation, activation of caspase­3 and ­8 and poly (ADP ribose) polymerase cleavage. Decreased levels of phosphorylated extracellular signal­regulated kinase (ERK) and phosphorylated AKT were also observed. Results indicate that the proliferation inhibitory effect of deltonin is associated with its apoptosis­inducing effect, which may correlate with ROS­mediated mitochondrial dysfunction as well as activation of the ERK/AKT signaling pathways. Therefore, deltonin may be a potential chemotherapeutic agent for the treatment of breast cancer.


Assuntos
Antineoplásicos Fitogênicos/toxicidade , Apoptose/efeitos dos fármacos , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Mitocôndrias/efeitos dos fármacos , Proteínas Proto-Oncogênicas c-akt/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Espirostanos/toxicidade , Antineoplásicos Fitogênicos/química , Neoplasias da Mama/metabolismo , Neoplasias da Mama/patologia , Caspase 3/metabolismo , Caspase 8/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Dioscorea/química , Feminino , Humanos , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Mitocôndrias/metabolismo , Proteína Quinase 1 Ativada por Mitógeno/metabolismo , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Poli(ADP-Ribose) Polimerases/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Transdução de Sinais/efeitos dos fármacos , Espirostanos/química , Proteína X Associada a bcl-2/metabolismo
6.
Chem Pharm Bull (Tokyo) ; 57(10): 1161-6, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19801882

RESUMO

Two new spirostanol glycosides (1, 2) and a new furostanol glycoside (3), together with nine known steroidal glycosides (4-12) were isolated from the leaves of Furcraea foetida (Agavaceae). The structures of the new compounds were determined by spectroscopic analysis and the results of hydrolytic cleavage. The isolated compounds were evaluated for their cytotoxic activities against HL-60 human leukemia cells, A549 human lung adenocarcinoma cells, HSC-2 human oral squamous carcinoma cells, and HSC-4 human oral squamous carcinoma cells.


Assuntos
Antineoplásicos Fitogênicos/química , Asparagaceae/química , Glicosídeos/química , Espirostanos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/toxicidade , Linhagem Celular Tumoral , Ensaios de Seleção de Medicamentos Antitumorais , Glicosídeos/isolamento & purificação , Glicosídeos/toxicidade , Células HL-60 , Humanos , Espectroscopia de Ressonância Magnética , Conformação Molecular , Espirostanos/isolamento & purificação , Espirostanos/toxicidade
7.
Vet Res Commun ; 25(3): 225-38, 2001 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-11334151

RESUMO

A sheep was dosed three times per day over six consecutive days with 70 g Narthecium ossifragum, and once on the seventh day with 70 g N. ossifragum. Additionally, it was dosed once on days 1-7 with 20 mg of [20,23,23-2H3]sarsasapogenin. After 7 days, the sheep was killed and GC-MS analysis of the free and conjugated sapogenin content in bile, urine, rumen, duodenum, jejunum, colon and rectum samples collected from the sheep, faecal samples collected on days 4-7, and dosed plant material was performed. The N. ossifragum contained mainly sarsasapogenin and smilagenin. Only neglible levels of deuterium-labelled sarsasapogenins were detected in the samples from the animal. Ingested saponins were quickly hydrolysed in the rumen to free sapogenins and, in part, epimerized at C-3 to afford episapogenins. The absorption of free sapogenins appeared to occur in the jejunum. The concentration of sapogenins in faeces reached a plateau 108 h after dosing started.


Assuntos
Plantas Tóxicas/metabolismo , Ovinos/metabolismo , Espirostanos/metabolismo , Animais , Fezes/química , Feminino , Absorção Intestinal , Jejuno/metabolismo , Fígado/metabolismo , Extratos Vegetais/metabolismo , Extratos Vegetais/farmacocinética , Extratos Vegetais/toxicidade , Saponinas/metabolismo , Saponinas/farmacocinética , Saponinas/toxicidade , Espirostanos/farmacocinética , Espirostanos/toxicidade
8.
Vet Hum Toxicol ; 42(3): 142-5, 2000 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-10839316

RESUMO

As part of an experimental study, crystal-associated cholangiopathy was induced in 9 sheep by grazing pure pastures of Brachiaria decumbens in Brazil. One of these sheep showed characteristic lesions of photosensitization. The analysis of the B decumbens samples by acidic hydrolysis followed by TLC and infrared spectrum revealed diosgenin as the principal sapogenin present in the plant. In the rumen contents samples from the B decumbens-grazing group were identified by TLC, 1H and 13C NMR and EIMS as epismilagenin, episarsasapogenin, and a mixture of smilagenin and sarsasapogenin. In the bile samples from the B decumbens-grazing group, TLC analysis demonstrated 2 compounds similar to epismilagenin and episarsasapogenin. However, by this same method, those compounds were not observed in the rumen contents and bile from 2 sheep which served as control animals. The P chartarum spore counts remained very low during the experimental period.


Assuntos
Colangite/veterinária , Hepatopatias/veterinária , Transtornos de Fotossensibilidade/veterinária , Poaceae/química , Sapogeninas/isolamento & purificação , Doenças dos Ovinos/etiologia , Animais , Ascomicetos/crescimento & desenvolvimento , Bile/química , Brasil , Colangite/induzido quimicamente , Colangite/etiologia , Cromatografia em Camada Fina/veterinária , Contagem de Colônia Microbiana/veterinária , Diosgenina/química , Diosgenina/isolamento & purificação , Diosgenina/toxicidade , Histocitoquímica , Fígado/patologia , Hepatopatias/etiologia , Hepatopatias/patologia , Espectroscopia de Ressonância Magnética , Espectrometria de Massas/veterinária , Transtornos de Fotossensibilidade/induzido quimicamente , Transtornos de Fotossensibilidade/etiologia , Poaceae/microbiologia , Poaceae/toxicidade , Rúmen/química , Sapogeninas/química , Sapogeninas/toxicidade , Ovinos , Doenças dos Ovinos/microbiologia , Doenças dos Ovinos/patologia , Espectrofotometria Infravermelho/veterinária , Espirostanos/química , Espirostanos/isolamento & purificação , Espirostanos/toxicidade , Esporos Fúngicos/crescimento & desenvolvimento
9.
Phytochemistry ; 50(5): 805-13, 1999 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-10192965

RESUMO

Chemical examination of the aerial parts of Dracaena draco has led to the isolation of a total of nine steroidal saponins, including five new ones. The structures of the new saponins were determined by spectral data and a few chemical transformations to be (23S,24S)-spirosta-5,25(27)-diene-1 beta,3 beta,23,24-tetrol 1-O-{O-(2,3,4-tri-O-acetyl-alpha-L-rhamnopyranosyl)-(1-->2)-alpha-L -arabinopyranosyl} 24-O-beta-D-fucopyranoside, (23S,24S)-spirosta-5,25(27)-diene-1 beta,3 beta, 23,24-tetrol 1-O-{O-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L -arabinopyranoside}, (23S,24S)-spirosta-5,25(27)-diene-1 beta,3 beta,23,24-tetrol 1-O-{O-(4-O- acetyl-alpha-L-rhamnopyranosyl)-(1-->2)-alpha-L-arabinopyransoide} , (23S)-spirosta-5,25(27)-diene-1 beta,3 beta,23-triol 1-O-{O-alpha-L- rhamnopyranosyl)-(1-->2)-alpha-L-arabinopyranoside} and (23S,24S)-spirosta-5,25(27)-diene-1 beta,3 beta,23-triol 1-O-{O-(4-O-acetyl-alpha-L-rhamnopyranosyl)-(1-->2)-alpha-L- arabinopyranoside}. The isolated saponins were evaluated for their cytostatic activity on leukemia HL-60 cells.


Assuntos
Antineoplásicos Fitogênicos/química , Células HL-60/efeitos dos fármacos , Plantas Medicinais , Saponinas/química , Espirostanos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/toxicidade , Sobrevivência Celular/efeitos dos fármacos , Humanos , Conformação Molecular , Estrutura Molecular , Saponinas/isolamento & purificação , Saponinas/toxicidade , Espirostanos/isolamento & purificação , Espirostanos/toxicidade
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