1.
Pol J Microbiol
; 57(2): 99-104, 2008.
Artigo
em Inglês
| MEDLINE
| ID: mdl-18646396
RESUMO
Ethambutol (EMB) is a first line drug in tuberculosis treatment inhibiting the biosynthesis of arabinogalactan, which is a component of the mycobacterial cell wall. The growth of Mycobacterium vaccae cells in the presence of EMB increases cell wall permeability, which was monitored by beta-sitosterol biotransformation. GC/MS and GLC/MS (gas chromatography/mass spectrometry) analysis revealed dramatic changes in the content of covalently bound mycolic acids and in molar ratio galactose (Gal) to arabinose (Ara) in the cell envelopes of EMB-treated cells. The detected variations in the compositions of fatty acids indicate that both the cell wall skeleton and outer layer (free lipids) are decomposed due to EMB treatment.
Assuntos
Antituberculosos/farmacologia , Esqueleto da Parede Celular/metabolismo , Etambutol/farmacologia , Galactanos/metabolismo , Mycobacterium/metabolismo , Polissacarídeos Bacterianos/metabolismo , Arabinose/metabolismo , Permeabilidade da Membrana Celular , Esqueleto da Parede Celular/efeitos dos fármacos , Galactanos/antagonistas & inibidores , Galactose/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Glicolipídeos/metabolismo , Mycobacterium/efeitos dos fármacos , Mycobacterium/crescimento & desenvolvimento , Ácidos Micólicos/metabolismo , Fosfolipídeos/metabolismo , Polissacarídeos Bacterianos/efeitos dos fármacos , Sitosteroides/metabolismo
2.
Periodontol 2000
; 10: 12-28, 1996 Feb.
Artigo
em Inglês
| MEDLINE
| ID: mdl-9567935