RESUMO
The genus Polygonatum Mill. belongs to the Liliaceae family, which is widely distributed all over the world. Modern studies have found that Polygonatum plants are very rich in chemical compounds such as saponins, polysaccharides and flavonoids. Steroidal saponins are the most commonly studied saponins in the genus Polygonatum and a total of 156 compounds have been isolated from 10 species of the genus. These molecules possess antitumor, immunoregulatory, anti-inflammatory, antibacterial, antiviral, hypoglycemic, lipid-lowering and anti-osteoporotic activities. In this review, we summarize recent advances in studies of the chemical constituents of steroidal saponins from Polygonatum, including their structural characteristics, possible biosynthetic pathways and pharmacological effects. Then, the relationship between the structure and some physiological activities is considered. This review aims to provide reference for further exploitation and utilization of the genus Polygonatum.
Assuntos
Vias Biossintéticas , Polygonatum , Saponinas , Esteroides , Antibacterianos/farmacologia , Anti-Inflamatórios/farmacologia , Antineoplásicos/farmacologia , Antivirais/farmacologia , Hipoglicemiantes/farmacologia , Polygonatum/química , Polygonatum/metabolismo , Saponinas/biossíntese , Saponinas/química , Saponinas/classificação , Saponinas/isolamento & purificação , Saponinas/farmacologia , Esteroides/biossíntese , Esteroides/química , Esteroides/classificação , Esteroides/isolamento & purificação , Esteroides/farmacologia , Relação Estrutura-Atividade , Humanos , AnimaisRESUMO
BACKGROUND: Emerging reports have shown the benefits of steroids in hospitalized COVID-19 patients as life-saving drugs. However, the use of steroids in COVID-19 patients is confusing among many physicians. AIM: The aim of the current study was to find out the exact association of steroids in the deaths of COVID-19 patients. METHODS: The relevant studies were searched in PubMed, Google scholar, and Clinical trials registries till May 25, 2021 and sorted out based on inclusion and exclusion criteria. The quality of studies was assessed using a standard scale. The pooled odds ratio was calculated with a 95% confidence interval. The sensitivity and sub-group analyses were also done. The publication bias was assessed qualitatively. The Rev Man 5 was used for all analyses with a random-effect model. RESULTS: The quantitative analysis was done with 9922 patients (6265-male and 3657-females) from 21 relevant studies. The pooled estimate results i.e. 0.52 [0.34, 0.80] have shown a significant reduction in deaths of COVID-19 patients in the steroidal group as compared to the non-steroidal group. The sensitivity analyses did not alter our conclusions. In subgroup analysis, methylprednisolone has shown a significant reduction in deaths of COVID-19 patients as compared to the non-steroidal group, however, more clinical evidence is required for dexamethasone and hydrocortisone. CONCLUSION: The use of steroids in hospitalized COVID-19 patients is useful to reduce deaths.
Assuntos
Tratamento Farmacológico da COVID-19 , COVID-19 , Metilprednisolona/uso terapêutico , COVID-19/mortalidade , Humanos , Mortalidade , SARS-CoV-2 , Esteroides/classificação , Esteroides/uso terapêuticoRESUMO
BACKGROUND: Nigella sativa L (NS) is a powerful antioxidant and medicinal plant with many therapeutic applications particularly in traditional medicine for respiratory, gastrointestinal, rheumatic, and inflammatory disorders, as well as cancer. OBJECTIVE: The aim of this study is to extract the active ingredients from the Moroccan Nigella sativa L and determine its antioxidant properties. We hypothesize that the separation of the compounds from Nigella sativa L has either a positive or negative effect on antioxidants. To study this, we explored different methods to simultaneously extract and separate compounds from Nigella sativa L and performed antioxidant tests (ß-carotene and DPPH) for all collected fractions. METHODS: Nigella sativa L was hot-extracted by Soxhlet and mother extracts and was separated using silica column chromatography with adequate eluents. Qualitative phytochemical tests to determine the chemical families in Nigella sativa L seeds were performed on the fractions. They were also identified and characterized by GC-MS and HPLC-DAD. Then, antioxidant activity was examined by ß-carotene bleaching and DPPH radical scavenger tests. Results and Conclusion. The mother extract hexane FH generated eight different fractions (SH1-8) and the acetone extract FA generated 11 fractions (SA1-11). The FH fractions had a high percentage of fatty acids, and the FA fractions had some interesting polyphenols derivative compounds. Phytochemical screening revealed secondary metabolites such as polyphenols flavonoids, alkaloids, steroids, terpenes coumarins, tannins, and saponins. We found that only two solvents (hexane, acetone) of different polarities could easily extract and simultaneously separate the components of Nigella sativa L. The antioxidant fractions that we collected had close activity to reference compounds but were more active than the corresponding mother extracts. Moreover, several IC50 values of fractions from acetone extract were better than those from hexane. Therefore, the antioxidant activity of Nigella sativa L is more attributed to flavonoids and polyphenols than fatty acids. In summary, the separation of hexane extract presents a more pronounced positive effect for antioxidant tests than acetone extract.
Assuntos
Antioxidantes/isolamento & purificação , Flavonoides/isolamento & purificação , Extração Líquido-Líquido/métodos , Nigella sativa/química , Compostos Fitoquímicos/isolamento & purificação , Polifenóis/isolamento & purificação , Sementes/química , Acetona/química , Alcaloides/química , Alcaloides/classificação , Alcaloides/isolamento & purificação , Antioxidantes/química , Antioxidantes/classificação , Compostos de Bifenilo/antagonistas & inibidores , Cromatografia Líquida de Alta Pressão , Cumarínicos/química , Cumarínicos/classificação , Cumarínicos/isolamento & purificação , Flavonoides/química , Flavonoides/classificação , Hexanos/química , Humanos , Marrocos , Compostos Fitoquímicos/química , Compostos Fitoquímicos/classificação , Picratos/antagonistas & inibidores , Extratos Vegetais/química , Plantas Medicinais , Polifenóis/química , Polifenóis/classificação , Saponinas/química , Saponinas/classificação , Saponinas/isolamento & purificação , Solventes/química , Esteroides/química , Esteroides/classificação , Esteroides/isolamento & purificação , Taninos/química , Taninos/classificação , Taninos/isolamento & purificação , Terpenos/química , Terpenos/classificação , Terpenos/isolamento & purificação , beta Caroteno/agonistasRESUMO
Importance: Most patients with primary aldosteronism, a major cause of secondary hypertension, are not identified or appropriately treated because of difficulties in diagnosis and subtype classification. Applications of artificial intelligence combined with mass spectrometry-based steroid profiling could address this problem. Objective: To assess whether plasma steroid profiling combined with machine learning might facilitate diagnosis and treatment stratification of primary aldosteronism, particularly for patients with unilateral adenomas due to pathogenic KCNJ5 sequence variants. Design, Setting, and Participants: This diagnostic study was conducted at multiple tertiary care referral centers. Steroid profiles were measured from June 2013 to March 2017 in 462 patients tested for primary aldosteronism and 201 patients with hypertension. Data analyses were performed from September 2018 to August 2019. Main Outcomes and Measures: The aldosterone to renin ratio and saline infusion tests were used to diagnose primary aldosteronism. Subtyping was done by adrenal venous sampling and follow-up of patients who underwent adrenalectomy. Statistical tests and machine-learning algorithms were applied to plasma steroid profiles. Areas under receiver operating characteristic curves, sensitivity, specificity, and other diagnostic performance measures were calculated. Results: Primary aldosteronism was confirmed in 273 patients (165 men [60%]; mean [SD] age, 51 [10] years), including 134 with bilateral disease and 139 with unilateral adenomas (58 with and 81 without somatic KCNJ5 sequence variants). Plasma steroid profiles varied according to disease subtype and were particularly distinctive in patients with adenomas due to KCNJ5 variants, who showed better rates of biochemical cure after adrenalectomy than other patients. Among patients tested for primary aldosteronism, a selection of 8 steroids in combination with the aldosterone to renin ratio showed improved effectiveness for diagnosis over either strategy alone. In contrast, the steroid profile alone showed superior performance over the aldosterone to renin ratio for identifying unilateral disease, particularly adenomas due to KCNJ5 variants. Among 632 patients included in the analysis, machine learning-designed combinatorial marker profiles of 7 steroids alone both predicted primary aldosteronism in 1 step and subtyped patients with unilateral adenomas due to KCNJ5 variants at diagnostic sensitivities of 69% (95% CI, 68%-71%) and 85% (95% CI, 81%-88%), respectively, and at specificities of 94% (95% CI, 93%-94%) and 97% (95% CI, 97%-98%), respectively. The validation series yielded comparable diagnostic performance. Conclusions and Relevance: Machine learning-designed combinatorial plasma steroid profiles may facilitate both screening for primary aldosteronism and identification of patients with unilateral adenomas due to pathogenic KCNJ5 variants, who are most likely to show benefit from surgical intervention.
Assuntos
Hiperaldosteronismo/tratamento farmacológico , Aprendizado de Máquina/tendências , Esteroides/classificação , Adulto , Feminino , Humanos , Hiperaldosteronismo/fisiopatologia , Masculino , Pessoa de Meia-Idade , Polônia , Esteroides/uso terapêuticoRESUMO
Acinetobacter baumannii is an emerging opportunistic pathogen that primarily infects critically ill patients in nosocomial settings and there is a need for identifying new alternative therapeutic agents against these organisms. Ceragenins are non-peptide, membrane-active agents that mimic the antimicrobial properties of antimicrobial peptides (AMPs) and affect the membrane permeability of microorganisms. The in vitro activities of CSA-8, CSA-13, CSA-44, CSA-131, CSA-138 either alone or in combination with colistin (sulphate) were determined against 25 carbapenem-resistant A. baumannii strains. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of selected ceragenins and colistin against these isolates were measured by in vitro microbroth dilution techniques. Checkerboard techniques and time-kill assays were performed to determine the activities of combinations. The MIC50 values (mg/L) of CSA-8, CSA-13, CSA-44, CSA-131, CSA-138 and colistin were 32, 4, 8, 2, 4 and 0.5, respectively. The MIC90 (mg/L) of CSA-8, CSA-13, CSA-44, CSA-131, CSA-138 and colistin were 128, 8, 16, 8, 16 and 16, respectively. At 6 h, 1×MIC and 2×MIC of CSA-13 were bactericidal. CSA-13 + colistin combination displayed synergistic interaction. Antagonism between antimicrobials was not observed. According to the results, CSA-13 and CSA-131 can be good alternatives for infections caused by carbapenem-resistant A. baumannii.
Assuntos
Acinetobacter baumannii/efeitos dos fármacos , Antibacterianos/farmacologia , Farmacorresistência Bacteriana , Viabilidade Microbiana/efeitos dos fármacos , Esteroides/farmacologia , Colistina/farmacologia , Sinergismo Farmacológico , Testes de Sensibilidade Microbiana , Esteroides/classificaçãoRESUMO
A new natural product, a 3,9-(1,2,3-trioxocine)-type steroid, named rauianodoxy (6), was isolated from Rauia nodosa, together with five steroids: sistostenone (1), stigmastenone (2), sitosterol (3), stigmasterol (4) and ergosterol peroxide (5), one coumarin, O-geranylosthenol (7), and three alkaloids, N-methylflindersine (8), zantobungeanine (9) and veprissine (10). Compounds 5-8 were isolated for the first time in the genus Rauia. These compounds were characterized on the basis of their spectral data, mainly one and two-dimensional NMR, and mass spectra, also involving comparison with the literature data. Theoretical studies at the DFT level reveal structural parameters for the 1,2,3-trioxole bridge compatible with known structures containing a similar group.
Assuntos
Alcaloides/química , Cumarínicos/química , Folhas de Planta/química , Esteroides/química , Triterpenos/química , Alcaloides/isolamento & purificação , Cumarínicos/classificação , Cumarínicos/isolamento & purificação , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Rutaceae/química , Espectrometria de Massas por Ionização por Electrospray , Esteroides/classificação , Esteroides/isolamento & purificação , Estigmasterol/químicaRESUMO
Saponins are an important class of plant natural products that consist of a triterpenoid or steroidal skeleton that is glycosylated by varying numbers of sugar units attached at different positions. Steroidal saponins are usually divided into two broad structural classes, namely spirostanol and furostanol saponins. A third, previously unrecognized structural class of plant saponins, the open-chain steroidal saponins, is introduced in this review; these possess an acyclic sidechain in place of the heterocyclic ring/s present in spirostanols and furostanols. Open-chain steroidal saponins are numerous and structurally diverse, with over 150 unique representatives reported from terrestrial plants. Despite this, they have to date been largely overlooked in reviews of plant natural products. This review catalogs the structural diversity of open-chain steroidal saponins isolated from terrestrial plants and discusses aspects of their structure elucidation, biological activities, biosynthesis, and distribution in the plant kingdom. It is intended that this review will provide a point of reference for those working with open-chain steroidal saponins and result in their recognition and inclusion in future reviews of plant saponins.
Assuntos
Plantas Medicinais/química , Saponinas , Esteroides , Estrutura Molecular , Saponinas/química , Saponinas/classificação , Saponinas/farmacologia , Esteroides/química , Esteroides/classificação , Esteroides/farmacologiaRESUMO
The history of isotope ratio mass spectrometry (IRMS) is briefly described. It is shown that the fundamental design of isotope ratio mass spectrometers has not changed since the 1940s. The basic findings concerning the natural variation of isotope abundances even date back to the 1930s. Recent improvements in the methodology mainly concern online coupling and analytical peripherals. The nature of isotopic scales necessitates a specific terminology which is unfamiliar to many analysts. However, corresponding guidelines exist that should be adopted by the anti-doping community. Currently, steroids represent the only group of compounds routinely analyzed by IRMS in doping-control. Suggestions are made in respect to a harmonized terminology concerning the nature and origins of steroids.
Assuntos
Anabolizantes/urina , Isótopos de Carbono/urina , Dopagem Esportivo , Cromatografia Gasosa-Espectrometria de Massas , Substâncias para Melhoria do Desempenho/urina , Esteroides/urina , Detecção do Abuso de Substâncias/métodos , Anabolizantes/classificação , Biomarcadores/urina , Isótopos de Carbono/história , Dopagem Esportivo/história , Cromatografia Gasosa-Espectrometria de Massas/história , História do Século XX , História do Século XXI , Humanos , Substâncias para Melhoria do Desempenho/classificação , Substâncias para Melhoria do Desempenho/história , Valor Preditivo dos Testes , Esteroides/classificação , Detecção do Abuso de Substâncias/história , Terminologia como AssuntoRESUMO
With the issuance of this Final Rule, the Administrator of the DEA classifies the following two steroids as "anabolic steroids'' under the Controlled Substances Act (CSA): prostanozol (17[beta]-hydroxy-5[alpha]-androstano[3,2-c]pyrazole) and methasterone (2[alpha],17[alpha]-dimethyl-5[alpha]-androstan-17[beta]-ol-3-one). These steroids and their salts, esters, and ethers are Schedule III controlled substances subject to the regulatory control provisions of the CSA.
Assuntos
Anabolizantes/classificação , Androstanóis/classificação , Controle de Medicamentos e Entorpecentes/legislação & jurisprudência , Esteroides/classificação , Rotulagem de Medicamentos/legislação & jurisprudência , Embalagem de Medicamentos/legislação & jurisprudência , Humanos , Estados UnidosRESUMO
Wastewater impoundments at concentrated animal feeding operations (CAFOs) represent a potential source of veterinary pharmaceuticals and steroid hormone contamination to shallow groundwater. This study investigates the occurrence of seventeen veterinary pharmaceuticals and thirteen steroid hormones and hormone metabolites in lagoons and adjacent groundwater at operating swine and beef cattle facilities. These sites were chosen because subsurface geology and previous monitoring of nitrate, ammonia and chloride levels in shallow ground water strongly indicated direct infiltration, and as such represent worst cases for ground water contamination by waste water. Pharmaceutical compounds detected in samples obtained from cattle facilities include sulfamerazine; sulfamethazine; erythromycin; monensin; tiamulin; and sulfathiazole. Lincomycin; ractopamine; sulfamethazine; sulfathiazole; erythromycin; tiamulin and sulfadimethoxine were detected in wastewater samples obtained from swine facilities. Steroid hormones were detected less frequently than veterinary pharmaceuticals in this study. Estrone, testosterone, 4-androstenedione, and androsterone were detected in wastewater impoundments at concentrations ranging from 30 to 3600ng/L, while only estrone and testosterone were detected in groundwater samples at concentrations up to 390ng/L. The co-occurrence of veterinary pharmaceutical and steroid hormone contamination in groundwater at these locations and the correlation between pharmaceutical occurrence in lagoon wastewater and hydraulically downgradient groundwater indicates that groundwater underlying some livestock wastewater impoundments is susceptible to contamination by veterinary pharmaceuticals and steroid hormones originating in wastewater lagoons.
Assuntos
Antibacterianos/análise , Hormônios/análise , Esteroides/análise , Drogas Veterinárias/análise , Gerenciamento de Resíduos/métodos , Poluentes Químicos da Água/análise , Animais , Antibacterianos/classificação , Bovinos , Monitoramento Ambiental , Hormônios/classificação , Gado , Esteroides/classificação , Suínos , Drogas Veterinárias/química , Drogas Veterinárias/classificaçãoRESUMO
Structures, taxonomic distribution and biological activities of steroid glycosides isolated from marine organisms over the last 8-10 years are reviewed. The bibliography includes 130 references.
Assuntos
Organismos Aquáticos/química , Glicosídeos , Esteroides , Animais , Glicosídeos/química , Glicosídeos/classificação , Glicosídeos/farmacologia , Biologia Marinha/métodos , Esteroides/química , Esteroides/classificação , Esteroides/farmacologiaRESUMO
Recent sequencing of amphioxus and sea urchin genomes has provided important data for understanding the origins of enzymes that synthesize adrenal and sex steroids and the receptors that mediate physiological response to these vertebrate steroids. Phylogenetic analyses reveal that CYP11A and CYP19, which are involved in the synthesis of adrenal and sex steroids, first appear in the common ancestor of amphioxus and vertebrates. This correlates with recent evidence for the first appearance in amphioxus of receptors with close similarity to vertebrate steroid receptors. Other CYP450 enzymes involved in steroid synthesis can be traced back to invertebrates, in which they have at least two functions: detoxifying xenobiotics and catalyzing the synthesis of sterols that activate nuclear receptors. CYP450 metabolism of hydrophobic xenobiotics may have been a key event in the origin of ligand-activated steroid receptors from constitutively active nuclear receptors.
Assuntos
Evolução Molecular , Receptores Citoplasmáticos e Nucleares/classificação , Receptores de Esteroides/classificação , Esteroide Hidroxilases/classificação , Esteroides/química , Animais , Colesterol/metabolismo , Cordados não Vertebrados/genética , Cordados não Vertebrados/metabolismo , Variação Genética , Genoma , Humanos , Ligantes , Filogenia , Receptores Citoplasmáticos e Nucleares/genética , Receptores Citoplasmáticos e Nucleares/metabolismo , Receptores de Esteroides/genética , Receptores de Esteroides/metabolismo , Esteroide Hidroxilases/genética , Esteroide Hidroxilases/metabolismo , Esteroides/classificação , Esteroides/metabolismo , Esteróis/metabolismo , Vertebrados/genética , Vertebrados/metabolismo , Xenobióticos/metabolismoRESUMO
INTRODUCTION: Steroidal saponins are the main active constituents in Ophiopogon japonicus Ker-Gawler (Liliaceae). However, because of their high polarity, non-chromophores and low content in plants, steroidal saponins are difficult to be isolated from O. japonicus by conventional phytochemical methods. OBJECTIVE: To develop a sensitive and rapid approach towards the structural analysis of steroidal saponins using HPLC/ESI-MS(n). METHODOLOGY: The fragmentation behaviors of six known steroidal saponins in negative ESI-MS(n) were used to deduce their mass spectral fragmentation mechanisms. By using HPLC/ESI-MS(n) , the important structural information on aglycone types, sugar types and saccharide sequences can be obtained. RESULTS: According to the HPLC retention behaviour, the molecular structural characteristics provided by multistage mass spectrometry spectra and the literature, a total of 8 steroidal saponins were tentatively identified or characterized in O. japonicus rapidly. CONCLUSION: This work has shown that HPLC-ESI-MS(n) may be used as an effective and rapid method for the characterization and identification of steroidal saponins from O. japonicus.
Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/química , Ophiopogon/química , Saponinas/química , Espectrometria de Massas por Ionização por Electrospray/métodos , China , Estrutura Molecular , Extratos Vegetais/química , Extratos Vegetais/classificação , Extratos Vegetais/isolamento & purificação , Raízes de Plantas/química , Saponinas/classificação , Saponinas/isolamento & purificação , Esteroides/química , Esteroides/classificação , Esteroides/isolamento & purificaçãoRESUMO
Liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (LC/ESI-QTOF-MS/MS) was performed to study the fragmentation behaviors of steroidal alkaloids from Fritillaria species, the antitussive and expectorant herbs widely used in traditional Chinese medicine. We propose, herein, a strategy that combining key diagnostic fragment ions and the relative abundances and amounts of major fragment ions (the ions exceeding 10% in abundance) to distinguish different sub-classes of Fritillaria alkaloids (FAs). It was found that hydrogen rearrangement and induction effects result in ring cleavage of the basic skeletons occurred in the MS/MS process and produced characteristic fragment ions, which are useful for structural elucidation. This method was finally used to investigate the primary steroidal alkaloids in the extracts of eight major Fritillaria species. As a result, 41 steroidal alkaloids (29 cevanine type, 1 jervine type, 6 veratramine type and 5 secosolanidine type alkaloids) were selectively identified in these Fritillaria species. Twenty-six compounds were unambiguously identified by comparing with the reference compounds and 15 compounds were tentatively identified or deduced according to their MS/MS data. Logical fragmentation pathways for different types of FAs have been proposed and are useful for the identification of these types of steroidal alkaloids in natural products especially when there are no reference compounds available.
Assuntos
Cromatografia Líquida/métodos , Fritillaria/química , Espectrometria de Massas por Ionização por Electrospray/métodos , Esteroides/química , Espectrometria de Massas em Tandem/métodos , Alcaloides de Veratrum/química , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/classificação , Esteroides/classificação , Alcaloides de Veratrum/classificaçãoRESUMO
Objetivo: Valorar el impacto en la calidad de vida de furoato demometasona, beclometasona y budesonida en pacientes asmáticos de las ciudades de Tehuacán y Camagüey. Material y método: Se asignaron aleatoriamente 80 niños asmáticos de ambas ciudades a casos y controles. Los primeros recibieron tratamiento con uno de los siguientes esteroides: furoato de mometasona 400 micro g, beclometasona 400 micro g, obudesonida 400 micro g. Los controles recibieron tratamiento a demanda. Todos fueron evaluados según los cuestionarios de calidad de vida SF-36 y AQLQ. Se determinaron las medias y se compararon antes y después utilizando la prueba de la t de Student. Se hizo un análisis de regresión para la correlación entre los resultados de ambos cuestionarios; se determinó la fuerza de asociación mediante la odds ratio (OR), y se eligió para el cuestionario SF-36 a los pacientes con mejor calidad de vida y para el AQLQ a los que habían incrementado 5 o más puntos en los ítems. Resultados: Mejoraron la calidad de vida 52 pacientes (p<0,05); de ellos, 31 utilizaron esteroides inhalados (OR= 3,11; intervalo de confianza [IC] del 95%: 1,18-8,19). La OR para Camagüey fue de 3,2 (IC del 95%: 0,7-14,9) y para Tehuacán de3,3 (IC del 95%: 1,08-10,4). En la comparación de medias entre muestras pareadas se observaron diferencias estadísticamente significativas para el SF-36 (p= 0,006) y el AQLQ (p <0,05). La correlación entre los resultados de ambos cuestionarios al final fue de 0,53 (p= 0,015).Conclusiones: La correlación entre los resultados de ambos cuestionarios al final del tratamiento fue significativa. Los asmáticos de Camagüey que recibieron dipropionato de beclometasona tenían una calidad de vida 2,2 veces más elevada que los que no la recibieron; los pacientes de Tehuacán que recibieron furoato de mometasona o budesonida disfrutaron de una calidad de vida 3,3 veces más elevada que los que no empleaban estos fármacos (AU)
Objective: To value the impact on life quality given by mometasonefuroate, beclomethasone and budesonide treatment in asthmatic patients from the cities of Tehuacán and Camagüey. Method and materials: 80 asthmatic children from both cities were randomly assigned to controls and cases. The first ones received treatment with one of the following steroids: mometasone furoate 400 micreg, beclomethasone 400 micreg or budesonide 400 micreg. Controls received treatment on demand. All of them were evaluated according to SF-36 and AQLQ quality of life scores. Averages were determined and they were comparedto each other before and after that, using the T of student test. A regression analysis was made for correlation between the results from both questionnaires. The association force was determined by odds ratio (OR), being chosen for theSF-36 questionnaire the patients who had the best quality of life and for the AQLQ those ones who had increased 5 points or more on the items. Results: 52 patients improved life quality (p <0.05); from them, 31 used inhaled steroids (OR 3,11; confidence interval [CI] of 95%: 1.18-8.19). The OR for Camagüey was 3.2 and CI of95%: 0.7-14.9 and for Tehuacán OR 3.3; IC of 95%: 1.08-10.4.In the comparison of averages between paired samples a statistically significant difference exist p= 0.006 for SF-36 and p <0.05 for AQLQ. The correlation between both questionnaires results at the end was 0.53 (p= 0.015). Conclusions: The correlation between the results of both questionnaires at the end of the treatment was significant. The asthmatic patients from Camagüey who used beclomethasone dipropionate have a quality of life increased in 2.2 times, comparing with those who do not use it. Those from Tehuacán who used beclomethasone dipropionate or budesonide enjoyed a 3.3 times higher quality of life than those who do not use it (AU)
Assuntos
Humanos , Masculino , Feminino , Criança , Qualidade de Vida , Asma/diagnóstico , Asma/prevenção & controle , Asma/terapia , Esteroides/classificação , Esteroides/farmacologia , Esteroides/uso terapêutico , Cuba/epidemiologia , México/epidemiologia , Beclometasona/classificação , Beclometasona/farmacologia , Beclometasona/uso terapêutico , Budesonida/classificação , Budesonida/farmacologia , Budesonida/uso terapêuticoRESUMO
A quantitative structure-property relationships (QSPR) study was carried out for 17 steroidal compounds using calculated molecular descriptors and measured properties. The utility of calculated molecular descriptors and properties was evaluated and improved in some instances by subgroup classification of these 17 compounds into estrogens and androgens. The calculated values for the octanol-water partition coefficient (logK(ow)) were found to be in good agreement with the measured values for all 17 compounds, whilst good agreement between the calculated and measured values for aqueous solubility (logS) was found only for the subgroup of androgens. Good linear relationships (R(2)0.782) were found between measured logK(ow) values and three molecular descriptors (logFOSA, hydrophobic component of the total solvent accessible surface area; logFISA, hydrophilic component of the total solvent accessible area and logPSA, Van de Waals surface area of polar nitrogen and oxygen atoms). For the measured logS values, only weak correlations with molecular descriptors were observed (R(2)0.505). The coefficient of logS in the relationship with the hydrophobic parameter (logFOSA) was negative but positive with the hydrophilic parameters (logFISA and logPSA). Conversely with logK(ow) the opposite was found. These observations are in accord with the effects of molecular polarity on aqueous solubility.
Assuntos
Octanóis/química , Esteroides/química , Poluentes Químicos da Água/química , Água/química , Algoritmos , Relação Quantitativa Estrutura-Atividade , Solubilidade , Esteroides/classificação , Propriedades de SuperfícieAssuntos
Cumarínicos/farmacologia , Ésteres/farmacologia , Limoninas/farmacologia , Plantas/química , Esteroides/farmacologia , Animais , Linhagem Celular Tumoral , Cumarínicos/síntese química , Cumarínicos/química , Cumarínicos/classificação , Ésteres/síntese química , Ésteres/química , Ésteres/classificação , Humanos , Limoninas/síntese química , Limoninas/classificação , Camundongos , Plantas Medicinais , Esteroides/síntese química , Esteroides/química , Esteroides/classificaçãoRESUMO
With the issuance of this final rule, the Deputy Administrator of the Drug Enforcement Administration (DEA) classifies the following three steroids as "anabolic steroids" under the Controlled Substances Act (CSA): Boldione, desoxymethyltestosterone, and 19-nor-4,9(10)-androstadienedione. These steroids and their salts, esters, and ethers are schedule III controlled substances subject to the regulatory control provisions of the CSA.