Assuntos
Doenças da Glândula Tireoide/imunologia , Carbimazol/uso terapêutico , Feminino , Doenças Fetais/tratamento farmacológico , Doenças Fetais/imunologia , Humanos , Recém-Nascido , Estimulador Tireóideo de Ação Prolongada/biossíntese , Troca Materno-Fetal , Gravidez , Complicações na Gravidez/tratamento farmacológico , Complicações na Gravidez/imunologia , Doenças da Glândula Tireoide/terapia , Tiroxina/uso terapêuticoAssuntos
Doença de Graves/radioterapia , Radioisótopos do Iodo/uso terapêutico , Estimulador Tireóideo de Ação Prolongada/biossíntese , Receptores de Superfície Celular/imunologia , Formação de Anticorpos/efeitos da radiação , Doença de Graves/tratamento farmacológico , Doença de Graves/imunologia , Humanos , Tireotropina/metabolismoAssuntos
Doença de Graves/imunologia , Estimulador Tireóideo de Ação Prolongada/biossíntese , Linfócitos/imunologia , Glândula Tireoide/imunologia , Adenilil Ciclases/análise , Antígenos de Superfície/imunologia , Membrana Celular/imunologia , Membrana Celular/ultraestrutura , Feminino , Humanos , Masculino , Frações Subcelulares/imunologia , Glândula Tireoide/enzimologia , Glândula Tireoide/ultraestruturaRESUMO
The lymphocytes from patients with Graves' disease or from healthy subjects have been cultured in vitro either alone or with phytohemagglutinin (PHA). After six days the culture supernatants have been assayed for their human thyroid-stimulating activity by measuring increases in adenosine 3',5' monophosphate (cyclic AMP) in human thyroid slices with which the supernatants have been incubated. Significant levels of human thyroid stimulator activity were found in the culture in which Graves' lymphocytes were cultured with PHA. This activity has been abolished by precipitation of the IgG from the culture supernatant with goat antihuman IgG serum. In contrast, when Graves' lymphocytes were cultured alone, or when control lymphocytes were cultured either alone or with PHA, there was no overall significant production of human thyroid-stimulating immunoglobulin (HTSI). It is concluded that Graves' lymphocytes can be stimulated by PHA to produce HTSI in vitro. Since PHA is known to stimulate only the T lymphocytes, which do not themselves elaborate immunoglobulins as the B lymphocytes do, the above observations indicate a cooperation between T and B lymphocytes in the production of HTSI, at least in this system.