RESUMO
An acetylenic C-glucuronide of the sedative-hypnotic drug ethchlorvynol was isolated from rabbit urine as the major metabolite. The C-glucuronide represents a novel metabolic pathway for acetylenes and is a rare example of the formation of a carbon-glucuronide bond in mammalian systems.
Assuntos
Etclorvinol/análogos & derivados , Etclorvinol/metabolismo , Acetileno , Animais , Radioisótopos de Carbono , Etclorvinol/urina , Glucuronidase , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Coelhos , Espectrofotometria InfravermelhoRESUMO
Hemoperfusion has been increasingly used to treat drug intoxication. Although efficient extraction rates have been reported with hemoperfusion devices, simultaneous measurements of drug adsorption on the columns and endogenous clearance rates have not been made. We studied three patients who ingested 12 to 22 g of ethchlorvynol (Placidyl). In each patient saline diuresis was induced, and Amberlite XAD-4 resin hemoperfusion was done. Resin extraction of ethchlorvynol was calculated hourly, and the recovery of drugs from each column was measured. Renal clearance of ethchlorvynol was simultaneously measured. The resin removed 100% of perfused ethchlorvynol measured by gas chromatography. Ethchlorvynol recovery from the columns agreed well with calculated extraction. Renal clearance was 1 to 6 mL/min, and estimated metabolic clearance was 16% to 31% of resin extraction rate. Complications included anemia, thrombocytopenia, hypocalcemia, and pancreatitis. Resin hemoperfusion substantially supplements endogenous clearance of ethchlorvynol and is a valuable therapy in ethchlorvynol intoxication.
Assuntos
Etclorvinol/intoxicação , Hemoperfusão/métodos , Resinas Vegetais , Adolescente , Contagem de Células Sanguíneas , Plaquetas , Cálcio/sangue , Etclorvinol/sangue , Etclorvinol/urina , Feminino , Hemoglobinas/análise , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
Two procedures for the detection of ethchlorvynol are presented. Procedure I involves the use of SA-2-cation-exchange resin loaded paper. The use of multiple ion-exchange resin papers is proposed in a sequence that enables the detection of amphetamines, barbiturates, phenothiazines, propoxyphene (Darvon), phencyclidine, cocaine (benzoyl ecgonine), pentazocine (Talwin) and benzodiazepines in addition to ethchlorvynol. This procedure discusses the details of detecting ethchlorvynol in combination with the entire array of drugs of abuse depending on the needs of a clinical program. Procedure II involves the principle of liquid-liquid extraction using either raw or spent urines.
Assuntos
Etclorvinol/urina , Transtornos Relacionados ao Uso de Substâncias/urina , Cromatografia por Troca Iônica , Cromatografia Líquida , Cromatografia em Papel , HumanosRESUMO
A simple method has been developed whereby chlormethiazole, ethchlorvynol and trichloroethanol can be simultaneously detected and measured in biological fluids. The procedure is based upon the rapid extraction of a small (50-microliter) sample volume with an equal volume of chloroform containing an internal standard, followed by the gas-liquid chromatographic analysis of this extract. Specimens of blood plasma or serum, urine and gastric contents can be used, and no interference from either endogenous or exogenous sources has been observed. The method is suitable for the measurement of the plasma concentrations of these compounds attained after overdosage.
Assuntos
Clormetiazol/intoxicação , Cloridrinas/intoxicação , Etclorvinol/intoxicação , Clormetiazol/sangue , Clormetiazol/urina , Cloridrinas/sangue , Cloridrinas/urina , Cromatografia Gasosa , Cromatografia Líquida , Etclorvinol/sangue , Etclorvinol/urina , Etilenocloroidrina/análogos & derivados , Humanos , Intoxicação/sangue , Intoxicação/urinaRESUMO
A rapid gas-liquid chromatographic method has been developed for use in the measurement of the hypnotic drug ethchlorvynol in small (50 microliter) volumes of either blood plasma or urine. Neither solvent transfer nor evaporation steps are used in the procedure and sources of interference have proved to be minimal. The method has been applied primarily to the analysis of specimens obtained from patients who had ingested an overdose of this drug. However with slight modification, the technique may be used in the measurement of the plasma concentrations of ethchlorvynol attained during therapy.
