RESUMO
A spectrophotometric method was developed for the determination of etidocaine hydrochloride (EH) in injectable pharmaceutical preparation. The proposal of this work was to develop a rapid, simple, inexpensive, precise and accurate visible spectrophotometric method. The method is based on the formation of the ion-pair complex by the EH reaction with bromocresol green in the pH 4.6 which after chloroform extraction gives a yellow color that in basic medium change to blue color and exhibits a maximum absorbance at 625 nm. The calibration graph was linear over the range 2.0-6.0 microg ml(-1) EH calculated on the final yellow solution. The R.S.D. of the slope of the four lines was 0.73%. This method can be applied to injectable pharmaceutical preparation dosage studied.
Assuntos
Anestesia Dentária , Anestésicos Locais/análise , Etidocaína/análise , Preparações Farmacêuticas/análise , Anestésicos Locais/química , Verde de Bromocresol/química , Soluções Tampão , Etidocaína/química , Reprodutibilidade dos Testes , Acetato de Sódio , EspectrofotometriaRESUMO
The method involves a single extraction of the drug and the internal standard, etidocaine, from alkalinized plasma with n-hexane. The gas chromatograph is equipped with a glass column (2.0 m x 2 mm) packed with 3% W/W SP2250 on Chromosorb W, (80/100 mesh) and a nitrogen sensitive detector. The method can accurately measure plasma bupivacaine concentrations down to 0.01 micrograms ml-1 using a sample size of 0.5 ml. The day-to-day variation of bupivacaine at 2.0 micrograms ml-1 is 6.90% (n = 10). The calibration graph is linear over the range 0.1-4.0 micrograms ml-1. The method is accurate, fast and sensitive and has been applied in a pharmacokinetic study of bupivacaine.
Assuntos
Bupivacaína/sangue , Absorção , Bupivacaína/isolamento & purificação , Bupivacaína/farmacocinética , Calibragem , Cromatografia Gasosa , Etidocaína/análise , Meia-Vida , Humanos , Concentração de Íons de Hidrogênio , Padrões de Referência , Distribuição TecidualRESUMO
A GLC method, employing a nitrogen-phosphorus-sensitive detector, is described for the analysis of mepivacaine, bupivacaine, etidocaine, lidocaine, and tetracaine in biological fluids. The method is simple, reliable, and sensitive, with a practical limit of sensitivity of approximately 2.5 ng/ml, well below therapeutic plasma levels. Extensive start-up procedures and sample preparation are not required.
