RESUMO
A method for spectrophotometric measurement of metronidasole and ethymisole jointly present in the urine has been developed. Combined polynomes have been designed to eliminate the effects of light-absorbing admixtures extracted from the urine on the results of analysis.
Assuntos
Etimizol/urina , Metronidazol/urina , Espectrofotometria Ultravioleta/métodos , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Fina , Humanos , Concentração de Íons de Hidrogênio , Espectrofotometria Ultravioleta/estatística & dados numéricosRESUMO
The metabolism of ethimizol in the human body has been investigated. Focus was on the detection and demonstration of the regioselective pathway of metabolic demethylation of ethimizol by determining the presence of the corresponding metabolites in blood, saliva and urine. Isolation, purification and identification of the metabolites present in the biological samples was achieved by applying a combination of the following methods: solid phase extraction, high performance liquid chromatography, high performance thin layer chromatography, nuclear magnetic resonance and mass spectrometry. The suggested chemical structures were definitely established by comparing the physicochemical characteristics of the ethimizol metabolites obtained from the individual biological fluids with the characteristics of synthetized authentic derivatives.
Assuntos
Etimizol/metabolismo , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Fina , Etimizol/sangue , Etimizol/urina , Humanos , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Valores de Referência , Saliva/químicaRESUMO
The chemical structure of an ethimizol metabolite found earlier in human urine and saliva was established as 4,5-dicarbamoyl-1-ethylimidazole (didesmethylethimizol). The treatment of biological samples comprised a solid-phase extraction, high-performance thin-layer chromatographic separation and high-performance liquid chromatographic purification techniques. The identification of the metabolite was based on the comparison of a mass spectrum of the substance isolated from the biological fluid with the mass spectra of synthetised reference compounds.
