RESUMO
Cross passive hemagglutination reactions between sulfanilic acid (SA), para-aminobenzoic acid (PABA), p-phenethidine (PT) and 4-aminoantipyrine (4-AA) haptens have been investigated using conjugates of diazo derivaties of these compounds with homologous serum proteins. Intense cross passive hemagglutination reactions were found between PABA, SA and 4-AA haptens. Although anti-PT sera cross reacted intensely with PABA, SA as well as with 4-AA haptens, antibodies cross reacting with PT were not detected in any of the anti-PABA, anti-SA and anti-4-AA antisera. The conjugates containing a heterologous hapten de not substantially alter the titer of the hemagglutination reactions performed with erythrocytes coated with the homologous hapten, but completely suppress the hemagglutination reactions performed with erythrocytes coated with this hapten as well as with other, but not all, heterologous, haptens. The results support the conclusion that the apparent polysensitization to drugs may actually rely upon cross reactions with haptens chemically related to the immunizing one.
Assuntos
Ácido 4-Aminobenzoico/imunologia , Aminobenzoatos/imunologia , Aminofenóis/imunologia , Ampirona/imunologia , Antipirina/análogos & derivados , Benzenossulfonatos/imunologia , Haptenos/imunologia , Fenetidina/imunologia , Ácidos Sulfanílicos/imunologia , Animais , Anticorpos/análise , Especificidade de Anticorpos , Reações Cruzadas , Feminino , Testes de Hemaglutinação , Masculino , CoelhosAssuntos
Alérgenos , Compostos de Anilina/imunologia , Fenetidina/imunologia , Abastecimento de Água , Anafilaxia/induzido quimicamente , Animais , Relação Dose-Resposta Imunológica , Eritrócitos/efeitos dos fármacos , Cobaias , Dose Letal Mediana , Leucócitos/efeitos dos fármacos , Camundongos , Fenetidina/toxicidade , Coelhos , Ratos , Fatores de TempoRESUMO
Guinea pigs were sensitized by p-phenetidine (PT), 2-hydroxy-p-phenetidine (HPT) as well as by conjugates prepared by reacting PT and HPT with proteins in vitro. Sensitization was evaluated by delayed skin reactivity and in vitro antigen-induced lymphocyte proleferation. HPT and HPT-protein conjugates were found to be the most effective sensitizing agents. Reaginic antibodies could be raised in both guinea pigs and rabbits by immunizing with PT- and HPT-protein conjugates but not by PT and HPT alone: these PCA antibodies showed strong cross-reactivity and could be elicited equally well with either the PT- or HPT-protein derivatives. By contrast, no precipitating antibodies could be raised in either species even after repeated immunizations over a period of 4 months. Peripheral blood lymphocytes, from a few patients who gave a positive patch test with PT, could be stiumlated in vitro with phenacetin and to a lesser degree with PT and with a HPT-derivative of human serum albumin.
