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1.
PLoS One ; 16(11): e0259518, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34727132

RESUMO

Spontaneous abortion is an impeding factor for the success rates of human assistant reproductive technology (ART). Causes of spontaneous abortion include not only the pregnant mothers' health conditions and lifestyle habits, but also the fetal development potential. Evidences had shown that fetal chromosome aneuploidy is associated with fetal spontaneous abortion, however, it is still not definite that whether other genome variants, like copy number variations (CNVs) or loss of heterozygosity (LOHs) is associated with the spontaneous abortion. To assess the relationship between the fetal genome variants and abortion during ART, a chromosomal microarray data including chromosomal information of 184 spontaneous aborted fetuses, 147 adult female patients and 78 adult male patients during ART were collected. We firstly analyzed the relationship of fetal aneuploidy with maternal ages and then compared the numbers and lengths of CNVs (< 4Mbp) and LOHs among adults and aborted fetuses. In addition to the already known association between chromosomal aneuploidy and maternal ages, from the chromosomal microarray data we found that the numbers and the accumulated lengths of short CNVs and LOHs in the aborted fetuses were significantly larger or longer than those in adults. Our findings indicated that the increased numbers and accumulated lengths of CNVs or LOHs might be associated with the spontaneous abortion during ART.


Assuntos
Feto Abortado/metabolismo , Variações do Número de Cópias de DNA/genética , Aborto Espontâneo , Feminino , Humanos , Perda de Heterozigosidade/genética , Masculino , Análise em Microsséries , Gravidez
2.
Eur J Med Genet ; 64(9): 104282, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34284163

RESUMO

The Forkhead transcription factor FOXG1 is a prerequisite for telencephalon development in mammals and is an essential factor controlling expansion of the dorsal telencephalon by promoting neuron and interneuron production. Heterozygous FOXG1 gene mutations cause FOXG1 syndrome characterized by severe intellectual disability, motor delay, dyskinetic movements and epilepsy. Neuroimaging studies in patients disclose constant features including microcephaly, corpus callosum dysgenesis and delayed myelination. Currently, investigative research on the underlying pathophysiology relies on mouse models only and indicates that de-repression of FOXG1 target genes may cause premature neuronal differentiation at the expense of the progenitor pool, patterning and migration defects with impaired formation of cortico-cortical projections. It remains an open question to which extent this recapitulates the neurodevelopmental pathophysiology in FOXG1-haploinsufficient patients. To close this gap, we performed neuropathological analyses in two foetal cases with FOXG1 premature stop codon mutations interrupted during the third trimester of the pregnancy for microcephaly and corpus callosum dysgenesis. In these foetuses, we observed cortical lamination defects and decreased neuronal density mainly affecting layers II, III and V that normally give rise to cortico-cortical and inter-hemispheric axonal projections. GABAergic interneurons were also reduced in number in the cortical plate and persisting germinative zones. Additionally, we observed more numerous PDGFRα-positive oligodendrocyte precursor cells and fewer Olig2-positive pre-oligodendrocytes compared to age-matched control brains, arguing for delayed production and differentiation of oligodendrocyte lineage leading to delayed myelination. These findings provide key insights into the human pathophysiology of FOXG1 syndrome.


Assuntos
Agenesia do Corpo Caloso/genética , Axônios/patologia , Fatores de Transcrição Forkhead/genética , Microcefalia/genética , Proteínas do Tecido Nervoso/genética , Transtornos do Neurodesenvolvimento/genética , Neurogênese , Oligodendroglia/patologia , Feto Abortado/metabolismo , Feto Abortado/patologia , Adulto , Agenesia do Corpo Caloso/patologia , Axônios/metabolismo , Encéfalo/embriologia , Encéfalo/metabolismo , Encéfalo/patologia , Códon sem Sentido , Feminino , Fatores de Transcrição Forkhead/metabolismo , Neurônios GABAérgicos/metabolismo , Neurônios GABAérgicos/patologia , Humanos , Interneurônios/metabolismo , Interneurônios/patologia , Microcefalia/patologia , Bainha de Mielina/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Transtornos do Neurodesenvolvimento/patologia , Oligodendroglia/metabolismo , Linhagem , Gravidez , Síndrome
3.
Mol Genet Genomic Med ; 9(2): e1563, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33491343

RESUMO

BACKGROUND: Neural tube defects (NTDs) are severe birth defects resulting from the failure of neural tube closure during embryogenesis. Both genetic and environmental factors contribute to the occurrence of NTDs and the heritability of NTDs is approximately 70%. As a key component of focal adhesions, Vinculin (VCL) plays pivotal roles in cell skeleton remodeling and signal transduction. Vcl deficient mice displayed NTD, but how VCL variants contribute to human NTDs has not been addressed yet. METHODS: We screened VCL variants in a Chinese cohort of 387 NTDs and 244 controls by targeted next-generation sequencing. RESULTS: We identified four case-specific VCL variations (p.M209L, p.D256fs, p.L555V and p.R586Q). VCL p.D256fs and p.L555V are novel variations that have never been reported. Our analysis revealed that p.D256fs is a loss-of-function variant, while p.L555V showed a gain of function in planner cell polarity (PCP) pathway regulation and cell migration, probably due to its enhanced protein stability. CONCLUSION: Our study reports human NTD specific novel variations in VCL and provides the functional evaluation of VCL variants related to the etiology of human NTDs.


Assuntos
Mutação com Ganho de Função , Mutação com Perda de Função , Defeitos do Tubo Neural/genética , Vinculina/genética , Feto Abortado/anormalidades , Feto Abortado/metabolismo , Adolescente , Feminino , Predisposição Genética para Doença , Células HEK293 , Humanos , Lactente , Masculino , Defeitos do Tubo Neural/metabolismo , Defeitos do Tubo Neural/patologia , Fenótipo
4.
Eur J Med Genet ; 63(4): 103775, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31568861

RESUMO

PIK3CA-Related Overgrowth Spectrum (PROS) encompass a group of disorders which are mainly characterized by segmental overgrowth of several tissues as well as venous and lymphatic malformations. It is caused by heterozygous, usually somatic mosaic, pathogenic variants in the PIK3CA gene. However, some patients presenting mainly isolated megalencephaly or "Cowden-like" features have been described harboring constitutional mutations of PIK3CA. Here, we report the case of a woman whose pregnancy was interrupted at 34 weeks of gestation after the detection of the following ultrasound abnormalities: left diaphragmatic hernia with intrathoracic stomach, right deviation of heart, intrathoracic double bubble sign, macrocephaly and polyhydramnios. Fetal autopsy contributed to better characterize the phenotype, showing megalencephaly, left diaphragmatic eventration, facial dysmorphism (hypertelorism, abnormal hair line implantation) and duplication of distal portion of the small bowel. Clinical exome sequencing identified a de novo constitutional variant c.1030G>A p.(Val344Met) in PIK3CA. Although this mutation has been previously described (as constitutional variant) in pediatric patients, our case represents the first detailed description of the prenatal features found in association with a constitutional PIK3CA mutation. Moreover, this case contributes to delineate novel features (diaphragmatic eventration and duplication of the distal part of the small bowel) which could be identified in association with PROS.


Assuntos
Anormalidades Múltiplas/patologia , Feto Abortado/patologia , Classe I de Fosfatidilinositol 3-Quinases/genética , Transtornos do Crescimento/patologia , Mutação , Anormalidades Múltiplas/genética , Feto Abortado/metabolismo , Adulto , Feminino , Transtornos do Crescimento/genética , Humanos , Fenótipo
5.
Epigenetics ; 14(12): 1234-1244, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31221015

RESUMO

Epigenetic mechanisms such as genomic imprinting have a fundamental role in embryo and fetal development. Hence, we here studied expression levels of epigenetic modifiers and imprinted genes in cases of ididopathic spontaneous abortion (SA). Thirty-five placental samples and 35 matched fetal tissues from second trimester SA were analysed; including 16 controls (placental and fetal infections as the known cause of spontaneous abortion) and 19 idiopathic SA cases. Transcript levels of epigenetic regulators and imprinted genes were measured by qRT-PCR and methylation at imprinted genes was studied by bisulfite genomic sequencing and MS-MLPA. Global DNA hydroxymethylation (5-hmC) levels were measured by an ELISA-based assay. We observed an upregulation of TET2 and TET3 in placental samples from idiopathic SA cases; however, no significant difference in global 5-hmC levels was observed. On the contrary, in fetal tissues, TET3 was markedly downregulated in idiopathic SA, showing an opposite trend to that observed in placental tissue. IGF2 and CDKN1C were upregulated and MEST downregulated in placentas from idiopathic SA cases; concordantly, IGF2 was also upregulated in fetal tissues from idiopathic SA cases. Although not reaching statistical significance, an increase in methylation levels of MEST, KvDMR1 and H19 DMRs was observed in idiopathic SA cases, concordantly with the observed changes in expression. Our study reveals, for the first time, deregulation of epigenetic modifiers and imprinted genes in both placental and fetal tissues from idiopathic SA cases in the second trimester of pregnancy, indicating a critical role during pregnancy.


Assuntos
Aborto Espontâneo/genética , Metilação de DNA , Impressão Genômica , 5-Metilcitosina/análogos & derivados , 5-Metilcitosina/metabolismo , Feto Abortado/metabolismo , Aborto Espontâneo/patologia , Adulto , Inibidor de Quinase Dependente de Ciclina p57/genética , Inibidor de Quinase Dependente de Ciclina p57/metabolismo , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Dioxigenases/genética , Dioxigenases/metabolismo , Feminino , Humanos , Fator de Crescimento Insulin-Like II/genética , Fator de Crescimento Insulin-Like II/metabolismo , Placenta/metabolismo , Gravidez , Segundo Trimestre da Gravidez , Proteínas Proto-Oncogênicas/genética , Proteínas Proto-Oncogênicas/metabolismo , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo
6.
Reprod Sci ; 26(11): 1485-1492, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-30791861

RESUMO

The adverse clinical result and poor treatment outcome in recurrent spontaneous abortion (RSA) make it necessary to understand the pathogenic mechanism. The mating combination CBA/J × DBA/2 has been widely used as an abortion-prone model compared to DBA/2-mated CBA/J mice. Here, we used RNA-seq to get a comprehensive catalogue of genes differentially expressed between survival placenta in abortion-prone model and control. Five hundred twenty-four differentially expressed genes were obtained followed by clustering analysis, Gene Ontology analysis, and pathway analysis. We paid more attention to immune-related genes namely "immune response" and "immune system process" including 33 downregulated genes and 28 upregulated genes. Twenty-one genes contribute to suppressing immune system and 7 are against it. Six genes were validated by reverse transcription-polymerase chain reaction, namely Ccr1l1, Tlr4, Tgf-ß1, Tyro3, Gzmb, and Il-1ß. Furthermore, Tlr4, Tgf-ß1, and Il-1ß were analyzed by Western blot. Such immune profile gives us a better understanding of the complicated immune processing in RSA and immunosuppression can rescue pregnancy loss.


Assuntos
Feto Abortado/imunologia , Aborto Espontâneo/genética , Aborto Espontâneo/imunologia , Modelos Animais de Doenças , Imunidade Celular/fisiologia , Mapas de Interação de Proteínas/fisiologia , Feto Abortado/metabolismo , Aborto Espontâneo/metabolismo , Animais , Feminino , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos CBA , Camundongos Endogâmicos DBA , Gravidez , Distribuição Aleatória , Especificidade da Espécie
7.
Cereb Cortex ; 29(5): 2115-2124, 2019 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-29688344

RESUMO

DACH1 is the human homolog of the Drosophila dachshund gene, which is involved in the development of the eye, nervous system, and limbs in the fly. Here, we systematically investigate DACH1 expression patterns during human neurodevelopment, from 5 to 21 postconceptional weeks. By immunodetection analysis, we found that DACH1 is highly expressed in the proliferating neuroprogenitors of the developing cortical ventricular and subventricular regions, while it is absent in the more differentiated cortical plate. Single-cell global transcriptional analysis revealed that DACH1 is specifically enriched in neuroepithelial and ventricular radial glia cells of the developing human neocortex. Moreover, we describe a previously unreported DACH1 expression in the human striatum, in particular in the striatal medium spiny neurons. This finding qualifies DACH1 as a new striatal projection neuron marker, together with PPP1R1B, BCL11B, and EBF1. We finally compared DACH1 expression profile in human and mouse forebrain, where we observed spatio-temporal similarities in its expression pattern thus providing a precise developmental description of DACH1 in the 2 mammalian species.


Assuntos
Corpo Estriado/embriologia , Corpo Estriado/metabolismo , Proteínas do Olho/metabolismo , Neocórtex/embriologia , Neocórtex/metabolismo , Neuroglia/metabolismo , Neurônios/metabolismo , Fatores de Transcrição/metabolismo , Feto Abortado/embriologia , Feto Abortado/metabolismo , Células Ependimogliais/metabolismo , Idade Gestacional , Humanos , Ventrículos Laterais/embriologia , Ventrículos Laterais/metabolismo , Células-Tronco Neurais/metabolismo , Células Neuroepiteliais/metabolismo , Prosencéfalo/embriologia , Prosencéfalo/metabolismo , Especificidade da Espécie
8.
Bratisl Lek Listy ; 117(6): 340-4, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27546366

RESUMO

OBJECTIVES: Identification of genetic association between the gene ERVW-1 and preeclampsia. BACKGROUND: Preeclampsia is a multifactorial disease affecting women during pregnancy and it is one of the main causes of perinatal and maternal morbidity and mortality. The pathophysiology of preeclampsia is very complex and several aspects of the disease have not been elucidated yet. Abnormal placentation frequently occurs during severe preeclampsia. Protein syncytin 1, a product of the ERVW-1 gene, plays a crucial role in the syncytiotrophoblast differentiation and optimal placentation. The syncytin 1 expression is disturbed during preeclampsia. The main focus of this study was the analysis of the ERVW-1 regulatory regions and identification of DNA polymorphisms associated with preeclamptic cases in Slovak population. METHODS: Regulatory region of gene ERVW-1 was analyzed by sequencing to identify genetic variants. RESULTS: We identified four DNA variants, namely rs4727276, rs148592540, rs569899772 and rs555416193, in samples of Slovak population. CONCLUSION: No relation between polymorphisms and preeclampsia was observed, indicating that further investigations with a larger sampling are still required. However, our work represents new original approach in genetic differential diagnosis of preeclampsia with possible useful findings in the future (Tab. 3, Fig. 1, Ref. 34).


Assuntos
Feto Abortado/metabolismo , Produtos do Gene env/genética , Pré-Eclâmpsia/genética , Proteínas da Gravidez/genética , Estudos de Casos e Controles , Feminino , Predisposição Genética para Doença , Humanos , Polimorfismo Genético , Polimorfismo de Nucleotídeo Único , Gravidez , Eslováquia
9.
J Biol Chem ; 291(9): 4386-98, 2016 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-26719341

RESUMO

The epigenetic regulation of spatiotemporal gene expression is crucial for human development. Here, we present whole-genome chromatin immunoprecipitation followed by high throughput DNA sequencing (ChIP-seq) analyses of a wide variety of histone markers in the brain, heart, and liver of early human embryos shortly after their formation. We identified 40,181 active enhancers, with a large portion showing tissue-specific and developmental stage-specific patterns, pointing to their roles in controlling the ordered spatiotemporal expression of the developmental genes in early human embryos. Moreover, using sequential ChIP-seq, we showed that all three organs have hundreds to thousands of bivalent domains that are marked by both H3K4me3 and H3K27me3, probably to keep the progenitor cells in these organs ready for immediate differentiation into diverse cell types during subsequent developmental processes. Our work illustrates the potentially critical roles of tissue-specific and developmental stage-specific epigenomes in regulating the spatiotemporal expression of developmental genes during early human embryonic development.


Assuntos
Encéfalo/embriologia , Cromatina/metabolismo , Epigênese Genética , Coração/embriologia , Histonas/metabolismo , Fígado/embriologia , Processamento de Proteína Pós-Traducional , Feto Abortado/metabolismo , Biomarcadores/metabolismo , Encéfalo/metabolismo , China , Cromatina/química , Bases de Dados de Ácidos Nucleicos , Regulação da Expressão Gênica no Desenvolvimento , Ontologia Genética , Biblioteca Genômica , Sequenciamento de Nucleotídeos em Larga Escala , Histonas/genética , Humanos , Fígado/metabolismo , Lisina/metabolismo , Metilação , Miocárdio/metabolismo , Especificidade de Órgãos , Análise de Sequência de DNA , Especificidade da Espécie
10.
Gynecol Endocrinol ; 31(9): 708-14, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26291815

RESUMO

PURPOSE: This study was performed on miscarriage samples for chromosome analysis to detect copy number variations (CNVs) related to subtelomeric regions, and with these results we aimed to adapt multiplex ligation-dependent probe amplification (MLPA) method for prenatal diagnosis. MATERIALS AND METHODS: The cell cultures and DNA isolations were performed on 60 miscarriage samples. For maternal contamination analysis, DNA isolations and quantitative fluorescent polymerase chain reactions were done using peripheric blood of mothers who had miscarriages. We compared short tandem repeat peak profiles of miscarriage samples and mothers. The subtelomeric regions of the chromosomes were assessed using the MLPA method. RESULTS: Of 43 miscarriage samples, 19 had normal karyotype (44.2%), 10 had numerical abnormalities (23.3%), and 2 had structural abnormalities (4.7%). Subtelomeric 16q duplication was determined in 2 of the 30 miscarriage samples investigated with MLPA method (6.6%). CONCLUSION: There is no statistically significant difference between two groups (p > 0.05). However, the fact that the 6.6% subtelomeric CNV found in miscarriage samples was not found in controls, showed that further studies are required. We recommend that the miscarriage samples of the couples with recurrent miscarriage should be analyzed in terms of subtelomeric CNV after the exclusion of other clinical reasons.


Assuntos
Feto Abortado/metabolismo , Aborto Espontâneo/genética , Variações do Número de Cópias de DNA/genética , Repetições de Microssatélites/genética , Aborto Habitual/genética , Adulto , Estudos de Casos e Controles , Aberrações Cromossômicas , Feminino , Humanos , Cariotipagem , Reação em Cadeia da Polimerase Multiplex , Reação em Cadeia da Polimerase , Gravidez
11.
Wien Klin Wochenschr ; 127(3-4): 98-102, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25398292

RESUMO

AIM: The purpose of this study was to investigate proliferative capacity of placenta previa percreta in the third trimester via evaluating Ki-67 proliferating index. METHODS: The paraffin blocks of placental tissues, which were obtained from the patients who underwent hysterectomy for placenta previa percreta (n = 12, gestational age > 28 weeks), from legal abortions (n = 12, gestational age < 10 weeks), and of cesarean deliveries with the indication of previous cesarean section, without any complication (n = 12, gestational age > 38 weeks), between January 2011 and April 2013, were included into the study. The paraffin blocks of the patients were stained with Ki-67 (proliferating cell marker) immunohistochemically, and Ki-67 proliferation index levels were calculated. RESULTS: Ki-67 proliferation index levels were higher in patients with legal abortions than patients with placenta percreta or noncomplicated cesarean delivery group. However, any statistically significant difference was not detected between the percreta and noncomplicated groups (p > 0.05). CONCLUSION: The tissue samples of the patients with placenta previa percreta exhibited low proliferative capacity similar to the samples of normal placentation group.


Assuntos
Antígeno Ki-67/metabolismo , Placenta Acreta/diagnóstico , Placenta Acreta/metabolismo , Placenta Prévia/diagnóstico , Placenta Prévia/metabolismo , Terceiro Trimestre da Gravidez/metabolismo , Feto Abortado/metabolismo , Adulto , Biomarcadores/metabolismo , Feminino , Humanos , Gravidez , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Índice de Gravidade de Doença
12.
Hum Mol Genet ; 22(6): 1218-32, 2013 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-23257287

RESUMO

Trisomy of chromosome 21 is associated to congenital heart defects in ∼50% of affected newborns. Transcriptome analysis of hearts from trisomic human foeti demonstrated that genes involved in mitochondrial function are globally downregulated with respect to controls, suggesting an impairment of mitochondrial function. We investigated here the properties of mitochondria in fibroblasts from trisomic foeti with and without cardiac defects. Together with the upregulation of Hsa21 genes and the downregulation of nuclear encoded mitochondrial genes, an abnormal mitochondrial cristae morphology was observed in trisomic samples. Furthermore, impairment of mitochondrial respiratory activity, specific inhibition of complex I, enhanced reactive oxygen species production and increased levels of intra-mitochondrial calcium were demonstrated. Seemingly, mitochondrial dysfunction was more severe in fibroblasts from cardiopathic trisomic foeti that presented a more pronounced pro-oxidative state. The data suggest that an altered bioenergetic background in trisomy 21 foeti might be among the factors responsible for a more severe phenotype. Since the mitochondrial functional alterations might be rescued following pharmacological treatments, these results are of interest in the light of potential therapeutic interventions.


Assuntos
Feto Abortado/metabolismo , Síndrome de Down/metabolismo , Fibroblastos/metabolismo , Cardiopatias Congênitas/metabolismo , Mitocôndrias/metabolismo , Síndrome de Down/complicações , Síndrome de Down/embriologia , Síndrome de Down/genética , Feminino , Cardiopatias Congênitas/complicações , Cardiopatias Congênitas/embriologia , Cardiopatias Congênitas/genética , Humanos , Masculino , Mitocôndrias/genética , Oxirredução , Estresse Oxidativo , Gravidez , Espécies Reativas de Oxigênio/metabolismo , Trissomia
13.
Genomics ; 101(3): 171-7, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23261704

RESUMO

The use of the anti-inflammatory drug indomethacin (INDO) in preterm infants has been associated with an increased risk of developing enteropathies. In this study, we have investigated the direct impact of INDO on the human mid-gestation intestinal transcriptome using serum-free organ culture. After determining the optimal dose of 1 µM of INDO (90% inhibition of intestinal prostaglandin E2 production and range of circulating levels in treated preterm babies), global gene expression profiles were determined using Illumina bead chip microarrays in both small and large intestines after 48 h of INDO treatment. Using Ingenuity Pathway Analysis software, we identified critical metabolic pathways that were significantly altered by INDO in both intestinal segments including inflammation and also glycolysis, oxidative phosphorylation and free radical scavenging/oxidoreductase activity, which were confirmed by qPCR at the level of individual genes. Taken together, these data revealed that INDO directly exerts multiple detrimental effects on the immature human intestine.


Assuntos
Indometacina/administração & dosagem , Redes e Vias Metabólicas/genética , Nascimento Prematuro/metabolismo , Transcriptoma/efeitos dos fármacos , Feto Abortado/metabolismo , Feto Abortado/patologia , Anti-Inflamatórios/administração & dosagem , Feminino , Humanos , Mucosa Intestinal/metabolismo , Intestinos/fisiopatologia , Técnicas de Cultura de Órgãos , Gravidez , Nascimento Prematuro/tratamento farmacológico , Nascimento Prematuro/fisiopatologia , Transcriptoma/genética
14.
Cell Reprogram ; 14(4): 324-33, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22775353

RESUMO

Mesenchymal stem cells (MSCs) have been isolated from many sources, including adults and fetuses. Previous studies have demonstrated that, compared with their adult counterpart, fetal MSCs with several remarkable advantages may be a better resource for clinical applications. In this study, we successfully isolated a rapidly proliferating cell population from limb bud of aborted fetus and termed them "human limb bud-derived mesenchymal stem cells" (hLB-MSCs). Characteristics of their morphology, phenotype, cell cycle, and differentiation properties were analyzed. These adherent cell populations have a typically spindle-shaped morphology. Flow cytometry analysis showed that hLB-MSCs are positive for CD13, CD29, CD90, CD105, and CD106, but negative for CD3, CD4, CD5, CD11b, CD14, CD15, CD34, CD45, CD45RA, and HLA-DR. The detection of cell cycle from different passages indicated that hLB-MSCs have a similar potential for propagation during long culture in vitro. The most novel finding here is that, in addition to their mesodermal differentiation (osteoblasts and adipocytes), hLB-MSCs can also differentiated into extramesenchymal lineages, such as neural (ectoderm) and hepatic (endoderm) progenies. These results indicate that hLB-MSCs have a high level of plasticity and can differentiate into cell lineages from all three embryonic layers in vitro.


Assuntos
Feto Abortado/citologia , Diferenciação Celular , Camadas Germinativas/citologia , Botões de Extremidades/citologia , Células-Tronco Mesenquimais/citologia , Feto Abortado/metabolismo , Animais , Antígenos de Diferenciação/metabolismo , Camadas Germinativas/metabolismo , Humanos , Botões de Extremidades/metabolismo , Células-Tronco Mesenquimais/metabolismo
15.
Purinergic Signal ; 8(2): 245-54, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22052556

RESUMO

The dorsal root ganglion (DRG) is consisted of neurons that relay multiple types of spinal sensory stimuli to the central nervous system. Several neuroactive molecules may be involved in sensory modulation especially pain processing at the DRG, including the purinergic receptor P2X3 and calcitonin-gene-related peptide (CGRP). P2X3 receptor has been considered a promising pharmaceutical target for the development of new pain medicine. Currently, litter is known about the expression of P2X3 in the human DRG. The present study characterized the localization of P2X3 in prenatal human DRG obtained from fetuses at 4-8 gestational months, by comparing to CGRP expression as well as binding pattern of isolectin-B4 (IB4), a marker of small DRG neurons presumably relevant to nociception. P2X3 immunoreactivity (IR) appeared in most neuron-like perikarya, with their numerical density reduced during the gestational period studied. P2X3 IR was co-labeled very commonly with IB4 binding and infrequently with CGRP IR and was not colocalized with IR for the gliocyte marker glutamine synthetase. Together, the data show an early and broad expression of P2X3 in prenatal human DRG neurons, pointing to a biological role of purinergic signaling during the development of spinal sensory system.


Assuntos
Gânglios Espinais/embriologia , Gânglios Espinais/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Receptores Purinérgicos P2X3/biossíntese , Feto Abortado/embriologia , Feto Abortado/metabolismo , Feminino , Humanos , Gravidez
16.
Semin Reprod Med ; 29(6): 463-9, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22161459

RESUMO

Miscarriage is a relatively common occurrence for otherwise healthy women. Despite its frequency, evaluation for cause is rare. The most common cause of miscarriage is sporadic chromosome errors. Chromosomal analysis of the miscarriage offers an explanation in at least 50% of cases. Conventional cytogenetic evaluation can only be done on fresh tissue, so it is critical that the treating physician consider genetic testing at the time of the miscarriage. Ultrasound can estimate the gestational age at the time of miscarriage and identify major abnormalities in some embryos. A careful pathological examination can add to the evaluation by ruling out rare disorders with the highest recurrence risk. A multidisciplinary approach to miscarriage evaluation is essential to understanding the cause and risk of recurrence. A thorough evaluation of a miscarriage, in combination with emotional support, can often provide the necessary reassurance and confidence as the patient prepares for her next pregnancy.


Assuntos
Feto Abortado/patologia , Aborto Espontâneo , Aberrações Cromossômicas , Placenta/patologia , Feto Abortado/irrigação sanguínea , Feto Abortado/metabolismo , Aborto Espontâneo/diagnóstico , Aborto Espontâneo/diagnóstico por imagem , Aborto Espontâneo/genética , Vilosidades Coriônicas/patologia , Aberrações Cromossômicas/embriologia , Cromossomos Humanos Par 16 , Cromossomos Humanos X , Decídua/patologia , Feminino , Humanos , Inflamação/patologia , Monossomia , Mosaicismo/embriologia , Gravidez , Primeiro Trimestre da Gravidez/genética , Primeiro Trimestre da Gravidez/metabolismo , Trissomia , Ultrassonografia
17.
Int J Hematol ; 93(6): 691-699, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21617888

RESUMO

Genetic correction of autologous hematopoietic stem cells has been proposed as an attractive treatment method for ß-thalassemia. Our previous study has shown that recombinant adeno-associated virus 2 (rAAV2) efficiently transduces human fetal liver hematopoietic cells, and mediates the expression of the human ß-globin gene in vivo. In this study, we investigated whether rAAV2 could also mediate the expression of normal ß-globin gene in human hematopoietic cells from ß-thalassemia patients. Human hematopoietic cells were isolated from aborted ß-thalassemia major fetuses, transduced with rAAV2-ß-globin, and then transplanted into nude mice. We found that rAAV2-ß-globin transduced human fetal hematopoietic cells, as determined by allele-specific PCR analysis. Furthermore, ß-globin transgene expression was detected in human hematopoietic cells up to 70 days post-transplantation in the recipient mice. High-pressure liquid chromatography analysis showed that human ß-globin expression levels increased significantly compared with control, as indicated by a 1.2-2.8-fold increase in the ratio of ß/α-globin chain. These novel data demonstrate that rAAV2 can transduce and mediate the normal ß-globin gene expression in fetal hematopoietic cells from ß-thalassemia patients. Our findings further support the potential use of rAAV-based gene therapy in the treatment of human ß-thalassemia.


Assuntos
Dependovirus/genética , Regulação da Expressão Gênica , Vetores Genéticos/genética , Células-Tronco Hematopoéticas/metabolismo , Globinas beta/genética , Globinas beta/metabolismo , Talassemia beta , Feto Abortado/metabolismo , Animais , Eritrócitos/metabolismo , Feminino , Técnicas de Transferência de Genes , Terapia Genética , Células HEK293 , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , RNA Mensageiro/genética , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Talassemia beta/genética , Talassemia beta/metabolismo
18.
J Physiol ; 589(Pt 11): 2827-45, 2011 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-21486766

RESUMO

Associations of neurological impairment with mutations in the thyroid hormone (TH) transporter, MCT8, and with maternal hypothyroxinaemia, suggest that THs are crucial for human fetal brain development. It has been postulated that TH transporters regulate the cellular supply of THs within the fetal brain during development. This study describes the expression of TH transporters in the human fetal cerebral cortex (7­20 weeks gestation) and during retinoic acid induced neurodifferentiation of the human N-Tera-2 (NT2) cell line, in triiodothyronine (T3) replete and T3-depleted media. Compared with adult cortex, mRNAs encoding OATP1A2, OATP1C1, OATP3A1 variant 2, OATP4A1, LAT2 and CD98 were reduced in fetal cortex at different gestational ages, whilst mRNAs encoding MCT8, MCT10, OATP3A1 variant 1 and LAT1 were similar. From the early first trimester, immunohistochemistry localised MCT8 and MCT10 to the microvasculature and to undifferentiated CNS cells. With neurodifferentiation, NT2 cells demonstrated declining T3 uptake, accompanied by reduced expressions of MCT8, LAT1, CD98 and OATP4A1. T3 depletion significantly reduced MCT10 and LAT2 mRNA expression at specific time points during neurodifferentiation but there were no effects upon T3 uptake, neurodifferentiation marker expression or neurite lengths and branching. MCT8 repression also did not affect NT2 neurodifferentiation. In conclusion, many TH transporters are expressed in the human fetal cerebral cortex from the first trimester, which could regulate cellular TH supply during early development. However, human NT2 neurodifferentiation is not dependent upon T3 or MCT8 and there were no compensatory changes to promote T3 uptake in a T3-depleted environment.


Assuntos
Feto Abortado/metabolismo , Proteínas de Transporte/metabolismo , Diferenciação Celular/fisiologia , Córtex Cerebral/embriologia , Neurônios/metabolismo , Células-Tronco Pluripotentes/metabolismo , Hormônios Tireóideos/metabolismo , Feto Abortado/embriologia , Sistema y+ de Transporte de Aminoácidos/genética , Sistemas de Transporte de Aminoácidos Neutros/genética , Sistemas de Transporte de Aminoácidos Neutros/metabolismo , Proteínas de Transporte/genética , Diferenciação Celular/efeitos dos fármacos , Linhagem Celular , Córtex Cerebral/metabolismo , Cadeias Leves da Proteína-1 Reguladora de Fusão/genética , Proteína-1 Reguladora de Fusão/genética , Proteína-1 Reguladora de Fusão/metabolismo , Expressão Gênica/efeitos dos fármacos , Expressão Gênica/genética , Idade Gestacional , Humanos , Proteínas de Filamentos Intermediários/genética , Transportador 1 de Aminoácidos Neutros Grandes/genética , Transportadores de Ácidos Monocarboxílicos/genética , Transportadores de Ácidos Monocarboxílicos/metabolismo , Proteínas do Tecido Nervoso/genética , Nestina , Proteínas de Neurofilamentos/genética , Neurônios/citologia , Fator 3 de Transcrição de Octâmero/genética , Transportadores de Ânions Orgânicos/genética , Células-Tronco Pluripotentes/citologia , Células-Tronco Pluripotentes/efeitos dos fármacos , RNA Interferente Pequeno/genética , Simportadores , Tretinoína/farmacologia , Tri-Iodotironina/metabolismo , Tri-Iodotironina/farmacologia
19.
Izv Akad Nauk Ser Biol ; (2): 235-9, 2011.
Artigo em Russo | MEDLINE | ID: mdl-21506398

RESUMO

The content of serum albumin and alpha-fetoprotein in the vitreous body of the eyes of human embryos from the 16th through the 24th week was investigated. It was detected that albumin and alpha-fetoprotein in the vitreous body of human eyes are presented in equal molar concentrations in the 16th week. There is 1.5-fold increased concentration of alpha-fetoprotein in comparison to albumin during the 17th week. Seventeen weeks later, there was a reduction in the concentration of both proteins. It was reported that cyanine dye, used for detection of albumin, does not interact with alpha-fetoprotein.


Assuntos
Albumina Sérica/metabolismo , Corpo Vítreo/embriologia , Corpo Vítreo/metabolismo , alfa-Fetoproteínas/metabolismo , Feto Abortado/metabolismo , Idade Gestacional , Humanos , Albumina Sérica/análise , Espectrometria de Fluorescência , Corpo Vítreo/química , alfa-Fetoproteínas/análise
20.
Bull Exp Biol Med ; 150(4): 420-1, 2011 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22268032

RESUMO

α-Fetoprotein was detected in human fetal vitreous body and its concentrations on gestation weeks 16-24 were measured. The concentration of α-fetoprotein was maximum during week 17 of pregnancy (17.4 mg/ml), but then decreased and reached 1.42 mg/ml by week 24.


Assuntos
Corpo Vítreo/embriologia , Corpo Vítreo/metabolismo , alfa-Fetoproteínas/análise , Feto Abortado/metabolismo , Feminino , Idade Gestacional , Humanos , Gravidez
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