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1.
J Neuroimmunol ; 361: 577725, 2021 12 15.
Artigo em Inglês | MEDLINE | ID: mdl-34610502

RESUMO

The acquired chronic demyelinating neuropathies include a growing number of disease entities that have characteristic, often overlapping, clinical presentations, mediated by distinct immune mechanisms, and responding to different therapies. After the discovery in the early 1980s, that the myelin associated glycoprotein (MAG) is a target antigen in an autoimmune demyelinating neuropathy, assays to measure the presence of anti-MAG antibodies were used as the basis to diagnose the anti-MAG neuropathy. The route was open for describing the clinical characteristics of this new entity as a chronic distal large fiber sensorimotor neuropathy, for studying its pathogenesis and devising specific treatment strategies. The initial use of chemotherapeutic agents was replaced by the introduction in the late 1990s of rituximab, a monoclonal antibody against CD20+ B-cells. Since then, other anti-B cells agents have been introduced. Recently a novel antigen-specific immunotherapy neutralizing the anti-MAG antibodies with a carbohydrate-based ligand mimicking the natural HNK-1 glycoepitope has been described.


Assuntos
Autoantígenos/imunologia , Doenças Autoimunes Desmielinizantes do Sistema Nervoso Central/imunologia , Glicoproteína Associada a Mielina/imunologia , Polirradiculoneuropatia/imunologia , Adenina/análogos & derivados , Adenina/uso terapêutico , Animais , Autoanticorpos/sangue , Autoanticorpos/imunologia , Subpopulações de Linfócitos B/imunologia , Antígenos CD57/imunologia , Doenças Autoimunes Desmielinizantes do Sistema Nervoso Central/diagnóstico , Doenças Autoimunes Desmielinizantes do Sistema Nervoso Central/terapia , Epitopos/imunologia , Transtornos Neurológicos da Marcha/imunologia , Humanos , Imunossupressores/uso terapêutico , Imunoterapia , Lenalidomida/uso terapêutico , Mamíferos , Camundongos , Mimetismo Molecular , Bainha de Mielina/química , Bainha de Mielina/imunologia , Bainha de Mielina/ultraestrutura , Fibras Nervosas Mielinizadas/imunologia , Fibras Nervosas Mielinizadas/patologia , Doença Autoimune do Sistema Nervoso Experimental/imunologia , Paraproteinemias/imunologia , Paraproteínas/imunologia , Piperidinas/uso terapêutico , Troca Plasmática , Polirradiculoneuropatia/diagnóstico , Polirradiculoneuropatia/terapia , Nós Neurofibrosos/química , Nós Neurofibrosos/imunologia , Ratos , Rituximab/uso terapêutico
2.
PLoS One ; 12(1): e0170847, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28125740

RESUMO

BACKGROUND: Optic neuritis (ON) in patients with anti-myelin oligodendrocyte glycoprotein (MOG)-IgG antibodies has been associated with a better clinical outcome than anti-aquaporin 4 (AQP4)- IgG ON. Average retinal nerve fiber layer thickness (RNFL) correlates with visual outcome after ON. OBJECTIVES: The aim of this study was to examine whether anti-MOG-IgG ON is associated with better average RNFL compared to anti-AQP4-IgG ON, and whether this corresponds with a better visual outcome. METHODS: A retrospective study was done in a consecutive cohort of patients following anti-AQP4-IgG and anti-MOG-IgG ON. A generalized estimating equation (GEE) models analysis was used to compare average RNFL outcomes in ON eyes of patients with MOG-IgG to AQP4-IgG-positive patients, after adjusting for the number of ON events. The final mean visual field defect and visual acuity were compared between ON eyes of MOG-IgG and AQP4-IgG-positive patients. A correlation between average RNFL and visual function was performed in all study eyes. RESULTS: Sixteen patients were analyzed; ten AQP4-IgG-positive and six MOG-IgG-positive. The six patients with MOG-IgG had ten ON events with disc edema, five of which were bilateral. In the AQP4-IgG-positive ON events, 1/10 patients had disc edema. Final average RNFL was significantly better in eyes following MOG-IgG-ON (75.33µm), compared to 63.63µm in AQP4-IgG-ON, after adjusting for the number of ON attacks (GEE, p = 0.023). Mean visual field defects were significantly smaller (GEE, p = 0.046) among MOG-IgG positive ON eyes compared to AQP-IgG positive ON eyes, but last visual acuity did not differ between the groups (GEE, p = 0.153). Among all eyes, average RNFL positively correlated with mean visual field defect (GEE, p = 0.00015) and negatively correlated with final visual acuity (GEE, p = 0.00005). CONCLUSIONS: Following ON, RNFL is better preserved in eyes of patients with MOG-IgG antibodies compared to those with AQP4-IgG antibodies, correlating with better visual outcomes.


Assuntos
Aquaporina 4/imunologia , Glicoproteína Mielina-Oligodendrócito/imunologia , Fibras Nervosas Mielinizadas/imunologia , Nervo Óptico/diagnóstico por imagem , Neurite Óptica/diagnóstico por imagem , Adulto , Aquaporina 4/genética , Autoanticorpos/biossíntese , Criança , Feminino , Expressão Gênica , Humanos , Imunoglobulina G/biossíntese , Pessoa de Meia-Idade , Glicoproteína Mielina-Oligodendrócito/genética , Fibras Nervosas Mielinizadas/patologia , Nervo Óptico/imunologia , Nervo Óptico/patologia , Neurite Óptica/imunologia , Neurite Óptica/patologia , Estudos Retrospectivos , Tomografia de Coerência Óptica , Acuidade Visual , Campos Visuais
3.
J Clin Immunol ; 34 Suppl 1: S86-104, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24740512

RESUMO

Schwann cells are the myelinating glial cells of the peripheral nervous system and establish myelin sheaths on large caliber axons in order to accelerate their electrical signal propagation. Apart from this well described function, these cells revealed to exhibit a high degree of differentiation plasticity as they were shown to re- and dedifferentiate upon injury and disease as well as to actively participate in regenerative- and inflammatory processes. This review focuses on the crosstalk between glial- and immune cells observed in many peripheral nerve pathologies and summarizes functional evidences of molecules, regulators and factors involved in this process. We summarize data on Schwann cell's role presenting antigens, on interactions with the complement system, on Schwann cell surface molecules/receptors and on secreted factors involved in immune cell interactions or para-/autocrine signaling events, thus strengthening the view for a broader (patho) physiological role of this cell lineage.


Assuntos
Sistema Imunitário/citologia , Fibras Nervosas Mielinizadas/imunologia , Sistema Nervoso Periférico/imunologia , Células de Schwann/imunologia , Animais , Apresentação de Antígeno , Comunicação Celular , Diferenciação Celular , Proteínas do Sistema Complemento/metabolismo , Humanos , Sistema Imunitário/imunologia , Proteínas de Membrana/imunologia , Sistema Nervoso Periférico/citologia , Transdução de Sinais
4.
Clin Perinatol ; 41(1): 83-103, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24524448

RESUMO

Chorioamnionitis (or placental infection) is suspected to be a risk factor for brain injury in premature infants. The suggested association between chorioamnionitis and cystic periventricular leukomalacia and cerebral palsy is uncertain because of the variability of study designs and definitions of chorioamnionitis. Improvements in neonatal intensive care may have attenuated the impact of chorioamnionitis on brain health outcomes. Large multicenter studies using rigorous definitions of chorioamnionitis on placental pathologies and quantitative magnetic resonance techniques may offer the optimal way to clarify the complex role of chorioamnionitis in modifying brain health and long-term outcomes.


Assuntos
Lesões Encefálicas/fisiopatologia , Paralisia Cerebral/fisiopatologia , Corioamnionite/fisiopatologia , Doenças Fetais/fisiopatologia , Leucomalácia Periventricular/fisiopatologia , Fibras Nervosas Mielinizadas/imunologia , Síndrome de Resposta Inflamatória Sistêmica/fisiopatologia , Lesões Encefálicas/etiologia , Lesões Encefálicas/imunologia , Paralisia Cerebral/etiologia , Paralisia Cerebral/imunologia , Corioamnionite/imunologia , Citocinas/imunologia , Feminino , Doenças Fetais/etiologia , Doenças Fetais/imunologia , Humanos , Recém-Nascido , Recém-Nascido Prematuro , Mediadores da Inflamação/imunologia , Leucomalácia Periventricular/etiologia , Leucomalácia Periventricular/imunologia , Gravidez , Síndrome de Resposta Inflamatória Sistêmica/etiologia , Síndrome de Resposta Inflamatória Sistêmica/imunologia
5.
Epilepsia ; 55(3): 414-22, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24502404

RESUMO

OBJECTIVE: Neuronal antibodies have been identified in patients with seizures as the main or sole symptom. Our aim was to investigate the prevalence of these autoantibodies in patients with focal epilepsy of unknown cause (FEoUC) and in the group having mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HS). METHODS: We studied anti-neuronal antibodies of consecutive adult patients diagnosed with FEoUC and MTLE-HS in our epilepsy center. The clinical and laboratory features of antibody-positive patients were compared with those of seronegative patients. The responses to therapy have also been investigated. RESULTS: Sera from 81 patients with epilepsy were tested. We found antibodies against glycine receptor (GLY-R) in 5 (6.2%), contactin-associated protein 2 (CASPR-2) in 4 (4.9%), N-methyl-d-aspartate receptor (NMDA-R) in 2 (2.5%), and voltage-gated potassium channel (VGKC)-complex in 2 (2.5%) of our patients with epilepsy. Psychotic attacks and nonspecific magnetic resonance imaging (MRI) white matter changes (WMCs) showed significant associations in seropositive patients (p = 0.003 and p = 0.03, respectively). Poor drug-response rates and total seizure counts were also higher in the seropositive patients but without reaching statistical significance. Three seropositive patients with previous epilepsy surgery showed typical histopathologic results for MTLE-HS, but not inflammatory changes. Moreover, some patients harboring these antibodies partly benefited from immunotherapy. SIGNIFICANCE: We detected neuronal antibodies in one sixth of patients with focal epilepsy, GLY-R antibodies being the leading one. Psychosis or nonspecific MRI WMCs were frequent in the seropositive group. Our results suggested that relevant antibodies should be screened for a treatment possibility in these groups.


Assuntos
Autoanticorpos/biossíntese , Epilepsias Parciais/diagnóstico , Epilepsias Parciais/imunologia , Fibras Nervosas Mielinizadas/metabolismo , Fibras Nervosas Mielinizadas/patologia , Neurônios/imunologia , Adulto , Autoanticorpos/sangue , Epilepsias Parciais/sangue , Feminino , Células HEK293 , Humanos , Imageamento por Ressonância Magnética/métodos , Masculino , Pessoa de Meia-Idade , Fibras Nervosas Mielinizadas/imunologia , Neurônios/metabolismo , Síndrome , Adulto Jovem
6.
Neurology ; 82(6): 470-3, 2014 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-24415573

RESUMO

OBJECTIVE: A serum antibody against the inward rectifying potassium channel KIR4.1 (KIR4.1-IgG) was recently discovered, which is found in almost half of adult patients with multiple sclerosis. We investigated the prevalence of KIR4.1-IgG in children with acquired demyelinating disease (ADD) of the CNS. We also compared antibody responses to KIR4.1 and myelin oligodendrocyte glycoproteins (MOGs), another potential autoantigen in childhood ADDs. METHODS: We measured KIR4.1-IgG by ELISA in children with ADD (n = 47), other neurologic disease (n = 22), and autoimmune disease (n = 22), and in healthy controls (HCs) (n = 18). One hundred six samples were also measured by capture ELISA. Binding of KIR4.1-IgG human subcortical white matter was analyzed by immunofluorescence. Anti-MOG antibodies were measured using a cell-based assay. RESULTS: KIR4.1-IgG titers were significantly higher in children with ADD compared with all control groups by ELISA and capture ELISA (p < 0.0001, p < 0.0001). Overall, 27 of 47 patients with ADD (57.45%) but none of the 62 with other neurologic disease or autoimmune disease or the HCs (0%) were KIR4.1-IgG antibody positive by ELISA. Sera containing KIR4.1-IgG stained glial cells in brain tissue sections. No correlation among KIR4.1-IgG, age, or MOG-IgG was observed in the ADD group. CONCLUSION: Serum antibodies to KIR4.1 are found in the majority of children with ADD but not in children with other diseases or in HCs. These findings suggest that KIR4.1 is an important target of autoantibodies in childhood ADD.


Assuntos
Autoanticorpos/imunologia , Encéfalo/imunologia , Imunoglobulina G/imunologia , Esclerose Múltipla/imunologia , Fibras Nervosas Mielinizadas/imunologia , Canais de Potássio Corretores do Fluxo de Internalização/imunologia , Adolescente , Doenças Autoimunes/imunologia , Estudos de Casos e Controles , Criança , Pré-Escolar , Doenças Desmielinizantes/imunologia , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Lactente , Masculino , Glicoproteína Mielina-Oligodendrócito/imunologia , Neuroglia/imunologia
8.
J Neuroinflammation ; 9: 156, 2012 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-22747960

RESUMO

BACKGROUND: In brain tissues from multiple sclerosis (MS) patients, clusters of activated HLA-DR-expressing microglia, also referred to as preactive lesions, are located throughout the normal-appearing white matter. The aim of this study was to gain more insight into the frequency, distribution and cellular architecture of preactive lesions using a large cohort of well-characterized MS brain samples. METHODS: Here, we document the frequency of preactive lesions and their association with distinct white matter lesions in a cohort of 21 MS patients. Immunohistochemistry was used to gain further insight into the cellular and molecular composition of preactive lesions. RESULTS: Preactive lesions were observed in a majority of MS patients (67%) irrespective of disease duration, gender or subtype of disease. Microglial clusters were predominantly observed in the vicinity of active demyelinating lesions and are not associated with T cell infiltrates, axonal alterations, activated astrocytes or blood-brain barrier disruption. Microglia in preactive lesions consistently express interleukin-10 and TNF-α, but not interleukin-4, whereas matrix metalloproteases-2 and -9 are virtually absent in microglial nodules. Interestingly, key subunits of the free-radical-generating enzyme NADPH oxidase-2 were abundantly expressed in microglial clusters. CONCLUSIONS: The high frequency of preactive lesions suggests that it is unlikely that most of them will progress into full-blown demyelinating lesions. Preactive lesions are not associated with blood-brain barrier disruption, suggesting that an intrinsic trigger of innate immune activation, rather than extrinsic factors crossing a damaged blood-brain barrier, induces the formation of clusters of activated microglia.


Assuntos
Encéfalo/imunologia , Encéfalo/metabolismo , Microglia/imunologia , Microglia/metabolismo , Adulto , Idoso , Idoso de 80 Anos ou mais , Encéfalo/patologia , Estudos de Coortes , Humanos , Imunidade Inata , Microglia/citologia , Pessoa de Meia-Idade , Esclerose Múltipla/imunologia , Esclerose Múltipla/metabolismo , Esclerose Múltipla/patologia , Fibras Nervosas Mielinizadas/imunologia , Fibras Nervosas Mielinizadas/metabolismo
9.
PLoS One ; 7(6): e39595, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22761835

RESUMO

Multiple sclerosis (MS) is a debilitating inflammatory disease of the central nervous system (CNS) characterized by local destruction of the insulating myelin surrounding neuronal axons. With more than 200 million MS patients worldwide, the absence of treatments that prevent progression or induce repair poses a major challenge. Anti-inflammatory therapies have met with limited success only in preventing relapses. Previous screening of human serum samples revealed natural IgM antibodies that bind oligodendrocytes and promote both cell signaling and remyelination of CNS lesions in an MS model involving chronic infection of susceptible mice by Theiler's encephalomyelitis virus and in the lysolecithin model of focal demyelination. This intriguing result raises the possibility that molecules with binding specificity for oligodendrocytes or myelin components may promote therapeutic remyelination in MS. Because of the size and complexity of IgM antibodies, it is of interest to identify smaller myelin-specific molecules with the ability to promote remyelination in vivo. Here we show that a 40-nucleotide single-stranded DNA aptamer selected for affinity to murine myelin shows this property. This aptamer binds multiple myelin components in vitro. Peritoneal injection of this aptamer results in distribution to CNS tissues and promotes remyelination of CNS lesions in mice infected by Theiler's virus. Interestingly, the selected DNA aptamer contains guanosine-rich sequences predicted to induce folding involving guanosine quartet structures. Relative to monoclonal antibodies, DNA aptamers are small, stable, and non-immunogenic, suggesting new possibilities for MS treatment.


Assuntos
Aptâmeros de Nucleotídeos/uso terapêutico , Axônios/efeitos dos fármacos , Doenças Desmielinizantes/tratamento farmacológico , Esclerose Múltipla/tratamento farmacológico , Bainha de Mielina/efeitos dos fármacos , Fibras Nervosas Mielinizadas/efeitos dos fármacos , Animais , Aptâmeros de Nucleotídeos/farmacologia , Axônios/patologia , Doenças Desmielinizantes/imunologia , Doenças Desmielinizantes/patologia , Modelos Animais de Doenças , Feminino , Camundongos , Esclerose Múltipla/imunologia , Esclerose Múltipla/patologia , Bainha de Mielina/imunologia , Bainha de Mielina/patologia , Fibras Nervosas Mielinizadas/imunologia , Fibras Nervosas Mielinizadas/patologia , Medula Espinal/efeitos dos fármacos , Medula Espinal/imunologia , Medula Espinal/patologia , Theilovirus/imunologia
10.
Glia ; 60(5): 751-60, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22337502

RESUMO

Both the central and the peripheral nervous systems are prone to multiple age-dependent neurological deficits, often attributed to still unknown alterations in the function of myelinating glia. To uncover the biological processes affected in glial cells by aging, we analyzed gene expression of the Schwann cell-rich mouse sciatic nerve at 17 time points throughout life, from day of birth until senescence. By combining these data with the gene expression data of myelin mouse mutants carrying deletions of either Pmp22, SCAP, or Lpin1, we found that the majority of age-related transcripts were also affected in myelin mutants (54.4%) and were regulated during PNS development (59.5%), indicating a high level of overlap in implicated molecular pathways. The expression profiles in aging copied the direction of transcriptional changes observed in neuropathy models; however, they had the opposite direction when compared with PNS development. The most significantly altered biological processes in aging involved the inflammatory/immune response and lipid metabolism. Interestingly, both these pathways were comparably changed in the aging optic nerve, suggesting that similar biological processes are affected in aging of glia-rich parts of the central and peripheral nervous systems. Our comprehensive comparison of gene expression in three distinct biological conditions including development, aging, and myelin disease thus revealed a previously unanticipated relationship among themselves and identified lipid metabolism and inflammatory/immune response pathways as potential therapeutical targets to prevent or delay so far incurable age-related and inherited forms of neuropathies.


Assuntos
Senescência Celular/imunologia , Metabolismo dos Lipídeos/imunologia , Fibras Nervosas Mielinizadas/imunologia , Fibras Nervosas Mielinizadas/metabolismo , Neuroglia/imunologia , Neuroglia/metabolismo , Transdução de Sinais/imunologia , Animais , Animais Recém-Nascidos , Camundongos , Camundongos da Linhagem 129 , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Vias Neurais/imunologia , Vias Neurais/metabolismo , Neuroglia/citologia , Nervo Isquiático/imunologia , Nervo Isquiático/metabolismo
11.
J Neuroimmunol ; 237(1-2): 93-7, 2011 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-21767883

RESUMO

Demyelinating diseases of the central nervous system (CNS) often include elevated IgG production in intrathecal space presenting as oligoclonal bands (OCBs) in cerebrospinal fluid (CSF). In most demyelinating diseases, e.g. in multiple sclerosis (MS), the underlying cause is not known. We used isoelectric focusing and affinity immunoblot to study the specificity of CSF OCBs to human herpesvirus-6 (HHV-6) in patients with demyelinating diseases of the CNS including MS. Eighty patients with positive OCB finding were included in the study. The OCBs reacted with the HHV-6 antigen in 18 cases (23%). Twelve of 46 MS patients (26%), 5 of 24 other demyelinating diseases (21%) and 1 of 10 other neurological disorders (10%) had HHV-6 specific OCBs in CSF. A specific intrathecal HHV-6 A and B antibody production was shown in a proportion of patients with demyelinating diseases and might suggest a role in the pathogenesis of these diseases.


Assuntos
Anticorpos Antivirais/líquido cefalorraquidiano , Herpesvirus Humano 6/imunologia , Esclerose Múltipla/imunologia , Esclerose Múltipla/virologia , Bandas Oligoclonais/líquido cefalorraquidiano , Infecções por Roseolovirus/imunologia , Adolescente , Adulto , Idoso , Antígenos Virais/imunologia , Doenças Autoimunes Desmielinizantes do Sistema Nervoso Central/líquido cefalorraquidiano , Doenças Autoimunes Desmielinizantes do Sistema Nervoso Central/imunologia , Doenças Autoimunes Desmielinizantes do Sistema Nervoso Central/virologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Esclerose Múltipla/líquido cefalorraquidiano , Proteínas da Mielina/imunologia , Fibras Nervosas Mielinizadas/imunologia , Infecções por Roseolovirus/complicações , Adulto Jovem
12.
J Neurotrauma ; 28(9): 1893-907, 2011 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21657851

RESUMO

Strategies that block infiltration of leukocytes into the injured spinal cord improve sparing of white matter and neurological recovery. In this article, we examine the dependency of recovery on hematogenous depletion of neutrophils and monocytes. Mice were depleted of neutrophils or monocytes by systemic administration of anti-Ly6G or clodronate-liposomes. A third group was depleted of both subsets. Neurological improvement, based on a battery of tests of performance, and white matter sparing, occurred only in animals depleted of both neutrophils and monocytes. We also attempted to define the nature of the environment that was favorable to recovery. Hemeoxygenase-1 and malondialdehyde, markers of oxidative stress and lipid peroxidation, respectively, were reduced to similar levels in animals depleted of both neutrophils and monocytes, or only monocytes, but remained elevated in the group only depleted of neutrophils. Matrix metalloproteinase-9, a protease involved in early damage, was most strongly reduced in animals depleted of both leukocyte subsets. Finally, disruption of the blood-spinal cord barrier and abnormal nonheme iron accumulation were reduced only in animals depleted of both neutrophils and monocytes. Together, these findings indicate cooperation between neutrophils and monocytes in mediating early pathogenesis in the contused spinal cord and defining long-term neurological recovery.


Assuntos
Monócitos/imunologia , Infiltração de Neutrófilos/imunologia , Neutrófilos/imunologia , Recuperação de Função Fisiológica/imunologia , Traumatismos da Medula Espinal/imunologia , Animais , Heme Oxigenase-1/metabolismo , Peroxidação de Lipídeos/imunologia , Malondialdeído/metabolismo , Camundongos , Monócitos/metabolismo , Monócitos/patologia , Fibras Nervosas Mielinizadas/imunologia , Fibras Nervosas Mielinizadas/patologia , Neutrófilos/metabolismo , Neutrófilos/patologia , Estresse Oxidativo/imunologia , Medula Espinal/imunologia , Medula Espinal/metabolismo , Medula Espinal/patologia , Traumatismos da Medula Espinal/metabolismo , Traumatismos da Medula Espinal/patologia
13.
Arch Neurol ; 68(4): 508-12, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21482930

RESUMO

OBJECTIVE: To describe the clinical, molecular, and neuropathological findings of a patient with aquaporin 4-positive relapsing myelitis who developed extensive brain involvement followed by death. DESIGN: Case report. SETTING: Foothills Medical Center, Calgary, Alberta, Canada. PATIENT: A 51-year-old woman with neuromyelitis optica spectrum disorder. RESULTS: Neuropathological examination disclosed neuromyelitis optica lesions, even in areas that appeared normal radiologically and grossly. Immunostaining confirmed the massive disintegration of astrocytes in the acute and chronic lesions, indicating that astrocytes are targeted early in the disease process. Induction of the immune response was demonstrated by reverse-transcriptase polymerase chain reaction analysis of relevant immune response genes. CONCLUSIONS: This article supports and supplements current concepts of astrocyte disintegration in neuromyelitis optica and of immune mechanisms in its pathogenesis.


Assuntos
Astrócitos/patologia , Encéfalo/patologia , Fibras Nervosas Mielinizadas/patologia , Neuromielite Óptica/diagnóstico , Neuromielite Óptica/patologia , Astrócitos/imunologia , Autopsia , Encéfalo/imunologia , Feminino , Humanos , Pessoa de Meia-Idade , Fibras Nervosas Mielinizadas/imunologia
14.
Anat Rec (Hoboken) ; 294(3): 487-93, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-21284091

RESUMO

The age-related changes, of the oligodendrocytes in rat subcortical white matter, were investigated in this study. The oligodendrocytes in subcortical white matter were labeled with anti-2',3'-cyclic nucleotide 3'-phosphodiesterase antibody (anti-CNPase antibody, a specific marker of oligodendrocytes). The total number of CNPase(+) cells was estimated with an unbiased stereological technique, the optical fractionator. In this study, we found that the total number of CNPase(+) cells in the young male rats and aged male rats was 14.4 ± 1.2 × 10(6) and 9.0 ± 1.0 × 10(6) , respectively. The total number of the CNPase(+) cells in the subcortical white matter of aged rats was significantly decreased by 37.5% when compared to young male rats. This study demonstrated that there was an aged-related decrease of the oligodendrocytes in subcortical white matter.


Assuntos
Envelhecimento/fisiologia , Córtex Cerebral/citologia , Córtex Cerebral/metabolismo , Fibras Nervosas Mielinizadas/metabolismo , Oligodendroglia/metabolismo , 2',3'-Nucleotídeo Cíclico Fosfodiesterases/metabolismo , Animais , Córtex Cerebral/imunologia , Técnicas Imunoenzimáticas , Masculino , Fibras Nervosas Mielinizadas/imunologia , Ratos , Ratos Wistar
15.
Mod Rheumatol ; 21(3): 290-5, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21188447

RESUMO

The aim of this study was to clarify the differences in the pathogenesis of neuropathy between myeloperoxidase-antineutrophil cytoplasmic antibody (MPO-ANCA)-positive and -negative patients with Churg-Strauss syndrome (CSS). Eight MPO-ANCA-positive and 14 MPO-ANCA-negative patients were included. In addition to the standard histology, nerve biopsies were examined, employing immunohistochemistry for eosinophil major basic protein and electron microscopy. The groups did not differ significantly in clinical profiles, including the peak disability score and number of blood eosinophils. In nerve biopsies, necrotizing vasculitis was found in 63% (5/8) of the ANCA-positive and 21% (3/14) of the ANCA-negative patients. Fibrinoid necrosis of vessel walls was noted in 4 ANCA-positive patients (50%), and in one ANCA-negative patient (p = 0039). In contrast, a large number of eosinophilic infiltrations in the epineurium was shown in 36% (5/14) of the ANCA-negative patients, with no eosinophilic infiltrations shown in ANCA-positive patients. In 3 ANCA-negative patients, endoneurial eosinophils were seen where focal axonal loss and capillary dilatation were occasionally noted. There may be 2 pathogenetic mechanisms of neuropathy with CSS: ANCA-related vascular fibrinoid necrosis, and a toxic eosinophilic effect on nerve fibers which is independent of ANCA. Therapy targeting activated eosinophils may be a possible treatment for intractable neuropathy of CSS.


Assuntos
Anticorpos Anticitoplasma de Neutrófilos/imunologia , Síndrome de Churg-Strauss , Eosinófilos/patologia , Doenças do Sistema Nervoso/imunologia , Doenças do Sistema Nervoso/patologia , Adulto , Idoso , Biópsia , Síndrome de Churg-Strauss/classificação , Síndrome de Churg-Strauss/imunologia , Síndrome de Churg-Strauss/patologia , Eosinófilos/ultraestrutura , Feminino , Humanos , Masculino , Microscopia Eletrônica , Pessoa de Meia-Idade , Necrose , Fibras Nervosas Mielinizadas/imunologia , Fibras Nervosas Mielinizadas/patologia , Fibras Nervosas Mielinizadas/ultraestrutura , Peroxidase/imunologia , Estudos Retrospectivos
16.
Intern Med ; 49(23): 2627-9, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-21139305

RESUMO

We describe a patient with painful neuropathy associated with an abnormal anti-MAG titer in which predominant involvement of intra-epidermal nerve fiber was documented. Electrophysiological studies revealed low-borderline amplitude of sensory and compound motor action potentials registering from lower limbs and normal conduction velocity. Sural nerve biopsy disclosed only a slight loss of myelinated fiber. Skin biopsy performed at the proximal thigh and at the distal leg was consistent with a non-length-dependent small fiber neuropathy. It is likely that in this case anti-MAG antibodies played a role in the pathogenesis of small fiber neuropathy.


Assuntos
Autoanticorpos/biossíntese , Glicoproteína Associada a Mielina/imunologia , Fibras Nervosas Mielinizadas/patologia , Polineuropatias/diagnóstico , Polineuropatias/imunologia , Autoanticorpos/sangue , Feminino , Humanos , Pessoa de Meia-Idade , Fibras Nervosas Mielinizadas/imunologia
17.
J Neuroimmunol ; 224(1-2): 101-7, 2010 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-20627412

RESUMO

Persistent infection of the central nervous system (CNS) of mice with the neuroadapted JHM strain of mouse hepatitis (MHV) is characterized by ongoing demyelination mediated by inflammatory T cells and macrophages that is similar both clinically and histologically with the human demyelinating disease multiple sclerosis (MS). Although extensive demyelination occurs in mice persistently infected with MHV there is only limited remyelination. Therefore, the MHV model of demyelination is a relevant model for studying disease and evaluating therapeutic approaches to protect cells of the oligodendrocyte lineage and promote remyelination. This concept is further highlighted as the etiology of MS remains enigmatic, but viruses have long been considered as potential triggering agents in initiating and/or maintaining MS symptoms. As such, understanding mechanisms associated with promoting repair within the CNS in the context of a persistent viral infection is critical given the possible viral etiology of MS. This review focuses on recent studies using either mouse neural stem cells (NSCs) or human oligodendrocyte progenitor cells (OPCs) derived from human embryonic stem cell (hESC) to promote remyelination in mice persistently infected with MHV. In addition, the potential role for chemokines in positional migration of transplanted cells is addressed.


Assuntos
Infecções por Coronavirus/terapia , Doenças Desmielinizantes/terapia , Doenças Desmielinizantes/virologia , Encefalite Viral/terapia , Vírus da Hepatite Murina/imunologia , Regeneração Nervosa/imunologia , Transplante de Células-Tronco/métodos , Animais , Linhagem da Célula/imunologia , Infecções por Coronavirus/imunologia , Infecções por Coronavirus/patologia , Doenças Desmielinizantes/patologia , Modelos Animais de Doenças , Encefalite Viral/imunologia , Encefalite Viral/patologia , Humanos , Camundongos , Fibras Nervosas Mielinizadas/imunologia , Fibras Nervosas Mielinizadas/metabolismo , Fibras Nervosas Mielinizadas/patologia , Transplante de Células-Tronco/tendências
19.
J Neuroimmunol ; 220(1-2): 79-89, 2010 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-20167380

RESUMO

Theiler's murine encephalomyelitis virus (TMEV) infection is a well-characterized model of multiple sclerosis (MS). Previous research has shown that chronic restraint stress (RS) during early TMEV infection exacerbates behavioral signs of the disease. The present data suggest that RS-induced increases in CNS inflammation, demyelination, and axonal degeneration may underlie this exacerbation. In addition, we report that males exhibit greater CNS inflammation and higher numbers of demyelinating lesions while females show greater susceptibility to RS-induced exacerbation. These findings indicate that RS during early TMEV infection increases CNS lesion formation during the late phase and suggest that the effects of RS are sex-dependent.


Assuntos
Infecções por Cardiovirus/imunologia , Sistema Nervoso Central/imunologia , Doenças Autoimunes Desmielinizantes do Sistema Nervoso Central/imunologia , Encefalomielite/imunologia , Estresse Psicológico/imunologia , Theilovirus/imunologia , Animais , Axônios/imunologia , Axônios/patologia , Axônios/virologia , Infecções por Cardiovirus/fisiopatologia , Sistema Nervoso Central/patologia , Sistema Nervoso Central/virologia , Doença Crônica , Doenças Autoimunes Desmielinizantes do Sistema Nervoso Central/fisiopatologia , Doenças Autoimunes Desmielinizantes do Sistema Nervoso Central/virologia , Modelos Animais de Doenças , Progressão da Doença , Encefalomielite/fisiopatologia , Encefalomielite/virologia , Feminino , Masculino , Camundongos , Fibras Nervosas Mielinizadas/imunologia , Fibras Nervosas Mielinizadas/patologia , Fibras Nervosas Mielinizadas/virologia , Restrição Física/efeitos adversos , Restrição Física/psicologia , Índice de Gravidade de Doença , Caracteres Sexuais , Estresse Psicológico/fisiopatologia , Degeneração Walleriana/imunologia , Degeneração Walleriana/patologia , Degeneração Walleriana/virologia
20.
Neuroscience ; 166(2): 464-75, 2010 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-20035837

RESUMO

Damage to oligodendrocyte (OL) progenitor cells (OPCs) and hypomyelination are two hallmark features of periventricular leukomalacia (PVL), the most common form of brain damage in premature infants. Clinical and animal studies have linked the incidence of PVL to maternal infection/inflammation, and activated microglia have been proposed to play a central role. However, the precise mechanism of how activated microglia adversely affects the survival and development of OPCs is still not clear. Here we demonstrate that lipopolysaccharide (LPS)-activated microglia are deleterious to OPCs, that is, impeding OL lineage progression, reducing the production of myelin basic protein (MBP), and mediating OPC death. We further demonstrate that LPS-activated microglia mediate OPC death by two distinct mechanisms in a time-dependent manner. The early phase of cell damage occurs within 24 h after LPS treatment, which is mediated by nitric oxide (NO)-dependent oxidative damage and is prevented by N(G)-nitro-l-arginine methyl ester (l-NAME), a general inhibitor of nitric oxide synthase. The delayed cell death is evident at 48 h after LPS treatment, is mediated by cytokines, and is prevented by blocking the activity of tumor necrosis factor-alpha (TNF-alpha) and pro-nerve growth factor (proNGF), but not by l-NAME. Furthermore, microglia-derived insulin-like growth factor-1 (IGF-1) and ciliary neurotrophic factor (CNTF) were significantly suppressed by LPS, and exogenous IGF-1 and CNTF synergistically protected OLs from death induced by LPS-treated microglia conditioned medium, indicating that a deficiency in trophic support may also be involved in OL death. Our finding that LPS-activated microglia not only induce two waves of cell death but also greatly impair OL development may shed some light on the mechanisms underlying selective white matter damage and hypomyelination in PVL.


Assuntos
Morte Celular/imunologia , Diferenciação Celular/imunologia , Linhagem da Célula/imunologia , Microglia/imunologia , Microglia/metabolismo , Oligodendroglia/imunologia , Células-Tronco/imunologia , Análise de Variância , Animais , Western Blotting , Sobrevivência Celular/imunologia , Células Cultivadas , Fator Neurotrófico Ciliar/imunologia , Fator Neurotrófico Ciliar/metabolismo , Ensaio de Imunoadsorção Enzimática , Imuno-Histoquímica , Fator de Crescimento Insulin-Like I/imunologia , Fator de Crescimento Insulin-Like I/metabolismo , Lipopolissacarídeos/imunologia , Lipopolissacarídeos/farmacologia , Microglia/efeitos dos fármacos , Fibras Nervosas Mielinizadas/imunologia , Fibras Nervosas Mielinizadas/metabolismo , Fator de Crescimento Neural/imunologia , Fator de Crescimento Neural/metabolismo , Óxido Nítrico/metabolismo , Oligodendroglia/metabolismo , Estresse Oxidativo/imunologia , Ratos , Espécies Reativas de Oxigênio/imunologia , Espécies Reativas de Oxigênio/metabolismo , Células-Tronco/metabolismo , Fator de Necrose Tumoral alfa/imunologia , Fator de Necrose Tumoral alfa/metabolismo
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