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1.
Work ; 78(2): 447-460, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38251085

RESUMO

BACKGROUND: Industrial workers often have musculoskeletal disorders due to the nature of their work. OBJECTIVE: The goal was to investigate the scientific use of polyherbal gel in relieving pain and stiffness due to musculoskeletal injuries and improving activities of daily living (ADLs) in industrial workers. METHODS: A pragmatic, single-blinded, randomized control study divided 200 musculoskeletal injury patients into four parallel groups (n = 50). Groups 1 and 2 were applied polyherbal gel via phonophoresis with therapeutic ultrasound and superficial massage. Groups 3 and 4 received diclofenac diethyl-ammonium 1% gel by phonophoresis and superficial massage. The Global Pain Relief Scale, Numeric Pain Rating Scale (NPRS), and Western Ontario and McMaster Universities Arthritis Index (WOMAC) were used to measure pain, stiffness, and ADLs. Data was analyzed using one-way analysis of variance (ANOVA) and paired t-test to compare mean±SD of four independent groups before and after gel application. The confidence interval was 95%, with p < 0.05 considered significant. RESULTS: The results revealed that polyherbal gel reduced pain (NPRS, WOMAC and Global pain relief scales) more efficiently (p≤0.000) when applied with phonophoresis as compared to applied with massage and standard diclofenac (p≤0.005), furthermore, polyherbal gel when applied with phonophoresis showed more efficient results. CONCLUSION: Industrial workers with musculoskeletal injuries benefited from the use of polyherbal gel for pain and inflammation relief. The polyherbal gel is natural, cost-effective, and easy to formulate.


Assuntos
Géis , Humanos , Adulto , Masculino , Feminino , Pessoa de Meia-Idade , Método Simples-Cego , Diclofenaco/administração & dosagem , Diclofenaco/uso terapêutico , Fonoforese/métodos , Doenças Musculoesqueléticas , Massagem/métodos , Atividades Cotidianas , Medição da Dor/métodos
2.
PLoS One ; 16(4): e0248356, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33835997

RESUMO

New and more efficient methods to sustainably intensify Aquaculture production are essential to attain the seafood demand for direct human consumption in the near future. Nutrition has been identified as one strategy of early exposure that might affect animal early development and later phenotype. This strategy may have positive consequences in the modulation of fish digestive physiology, which will correlate with higher performance outputs. Thus, improving fish digestive efficiency will lead to higher productivity and lower biogenic emission from aquaculture facilities, minimising the impact on the environment while increasing the biological efficiency. An innovative in ovo nutritional modulation technique based on low-frequency ultrasounds was used to enhance the transport of amino acids across the embryo membranes. An early stimulus with either arginine or glutamine, both involved in gut maturation, was applied in zebrafish (Danio rerio) embryos at 3.5 hours post-fertilization (hpf). At 22 days post-fertilization (dpf), growth performance, digestive enzyme activities and gut microbiota composition were analysed to evaluate the larval nutrition-induced metabolic plasticity and the effects on fish digestive efficiency. Results showed that fish survival was not affected either by the sonophoresis technique or amino acid supplementation. Final dry weight at 22 dpf was statistically higher in larvae from glutamine treatment when compared to the control even with lower trypsin activity, suggesting a higher nutrient digestion capacity, due to a slightly modulation of gut microbiota. Higher arginine supplementation levels should be tested as strategy to enhance growth at later developmental stages. In conclusion, this study demonstrated the efficiency of sonophoresis technique for in ovo nutritional modulation and suggests that in ovo glutamine supplementation might promote growth at later developmental stage through a positive microbiota modulation.


Assuntos
Aminoácidos/farmacologia , Larva/metabolismo , Fonoforese/métodos , Aminoácidos/metabolismo , Animais , Arginina/metabolismo , Arginina/farmacologia , Vias de Administração de Medicamentos/veterinária , Embrião não Mamífero/metabolismo , Glutamina/metabolismo , Glutamina/farmacologia , Larva/efeitos dos fármacos , Fenômenos Fisiológicos da Nutrição/fisiologia , Peixe-Zebra/embriologia , Peixe-Zebra/genética , Proteínas de Peixe-Zebra/metabolismo
3.
Drug Deliv ; 27(1): 1087-1093, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32706279

RESUMO

The aim of this study was to evaluate the intensity of sonophoresis at which the skin penetration of celecoxib was enhanced and to study the combined effects of sonophoresis and microemulsion application on the dermal delivery of celecoxib. The sonophoresis intensity that provided the highest skin penetration enhancement of celecoxib was 30 Watts/cm2. However, the combination of sonophoresis and the microemulsion resulted in a decrease in celecoxib skin penetration. The results of a confocal laser scanning microscopy study using the colocalization analysis of multifluorescently labeled particles revealed that the reduction in skin penetration of celecoxib from the combination of sonophoresis and a microemulsion resulted from a decrease in transfollicular penetration, which is the major skin absorption pathway of the microemulsion.


Assuntos
Celecoxib/administração & dosagem , Inibidores de Ciclo-Oxigenase 2/administração & dosagem , Emulsões , Fonoforese/métodos , Pele/metabolismo , Administração Cutânea , Animais , Celecoxib/farmacocinética , Inibidores de Ciclo-Oxigenase 2/farmacocinética , Sus scrofa
4.
Naunyn Schmiedebergs Arch Pharmacol ; 393(12): 2293-2300, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-32653977

RESUMO

The aim of this study is to investigate the effects of limonene, alone or associated with therapeutic ultrasound, on oxidative stress following skeletal muscle injury. Thirty male Wistar rats were divided into 5 groups: CTR-control, MI-muscle injury without treatment, TPU-therapeutic pulsed ultrasound alone, TPU + LIM-phonophoresis with 5% limonene, and LIM-5% limonene applied topically. Muscle injury was induced by a mechanical abrupt impact over gastrocnemius muscle. The animals were treated in the following intervals: 2, 12, 24, 48, 72, and 96 h after injury. Blood and gastrocnemius samples were collected 98 h after lesion for data analysis. Creatine kinase (CK) and lactate dehydrogenase (LDH) activity, lipid peroxidation (TBARS) levels, catalase (CAT), and superoxide dismutase (SOD) activity were assessed. CK (p = 0.01), SOD activity (p < 0.01), and TBARS levels (p < 0.01) were increased after injury. There was no effect on LDH levels in any group. Phonophoresis (TABRS p < 0.01; SOD p = 0.01), TPU alone (TBARS p < 0.01; SOD p = 0.01), and LIM alone (TBARS p < 0.01; SOD p < 0.01) reduced TBARS levels and SOD activity after muscle injury. There was no change for CAT activity after injury. Only phonophoresis reduced CK activity after injury (p < 0.01). There was no difference between phonophoresis, TPU alone and LIM alone groups for TBARS, SOD, CAT, and LDH. Limonene alone and TPU alone were effective in reducing oxidative stress parameters after skeletal muscle injury. Only phonophoresis decreased CK activity. Skeletal muscle injury increases reactive oxidative species (ROS) levels and muscle proteins activity as creatine kinase (CK) and lactate dehydrogenase (LDH). Five percent limonene, alone or associated with therapeutic pulsed ultrasound, exhibited reduction of CK, superoxide dismutase (SOD) and catalase (CAT) activity, and lipid peroxidation markers (TBARS). Graphical abstract.


Assuntos
Antioxidantes/administração & dosagem , Limoneno/administração & dosagem , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/lesões , Estresse Oxidativo/efeitos dos fármacos , Fonoforese/métodos , Administração Tópica , Animais , Limoneno/metabolismo , Masculino , Músculo Esquelético/metabolismo , Estresse Oxidativo/fisiologia , Ratos , Ratos Wistar
5.
J Athl Train ; 55(6): 628-635, 2020 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-32320285

RESUMO

CONTEXT: The effect of ultrasound frequency on phonophoresis drug delivery in humans is unknown. OBJECTIVE: To determine if a low (45-kHz) or high (1-MHz) frequency delivered a higher dexamethasone (Dex) concentration through the skin. DESIGN: Controlled laboratory study. SETTING: Laboratory. PATIENTS OR OTHER PARTICIPANTS: A total of 40 healthy men between the ages of 18 and 45 years (age = 23.1 ± 2.6 years, height = 176.1 ± 7.2 cm, mass = 88.5 ± 19.4 kg, posterior calf subcutaneous thickness measured using musculoskeletal ultrasound imaging = 0.6 ± 0.2 cm). INTERVENTION(S): Participants were randomly assigned to 1 of 4 groups (ultrasound frequency at microdialysis probe depth): (1) 45-kHz frequency at 1 mm, (2) 45-kHz frequency at 4 mm, (3) 1-MHz frequency at 1 mm, or (4) 1-MHz frequency at 4 mm (n = 10 in each group). Three linear microdialysis probes were inserted at the desired tissue depth. We rubbed dexamethasone sodium phosphate (Dex-P) into the skin and then applied a 15-minute phonophoresis treatment. MAIN OUTCOME MEASURE(S): Dialysate was collected during the treatment and 60 minutes posttreatment and analyzed for Dex-P, Dex, and the metabolite form of Dex. The sum of the 3 analytes was calculated as total dexamethasone (Dex-total), and differences between the 45-kHz and 1-MHz treatment groups were determined by a repeated-measures analysis of variance. RESULTS: At 1 mm, 3 (30%) participants in the 45-kHz and 4 (40%) participants in the 1-MHz group had measurable levels of Dex-P. Total dexamethasone increased after the treatment ceased, independent of ultrasound frequency (P < .001), with a trend of the 45-kHz treatment to produce a greater increase in drug concentration (P = .006). At 4 mm, 5 (50%) participants in the 45-kHz and 1 (10%) participant in the 1-MHz group had measurable levels of Dex-P. We observed no difference in Dex-total concentration between treatment groups at 4 mm (P = .72). CONCLUSIONS: Phonophoresis provided a mechanism for Dex-total delivery at the 1- and 4-mm tissue depths. However, the effectiveness of the ultrasound frequencies varied between the 2 measured tissue depths.


Assuntos
Dexametasona , Fonoforese/métodos , Adulto , Anti-Inflamatórios/administração & dosagem , Anti-Inflamatórios/farmacocinética , Disponibilidade Biológica , Dexametasona/administração & dosagem , Dexametasona/farmacocinética , Monitoramento de Medicamentos/métodos , Glucocorticoides/administração & dosagem , Glucocorticoides/farmacocinética , Voluntários Saudáveis , Humanos , Masculino , Avaliação de Resultados em Cuidados de Saúde , Fatores de Tempo
6.
J Bodyw Mov Ther ; 24(1): 15-18, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31987536

RESUMO

OBJECTIVE: This study examined the effects of treatment with Phyllanthus amarus nanoparticle gel applied by phonophoresis (PP) and ultrasound therapy (UT) in patients with symptomatic knee osteoarthritis (OA) using a randomized, double-blind, controlled trial. METHODS: Patients with knee OA (n = 40; mean age ±â€¯SD, 64.30 ±â€¯9.71 years), who had visual analogue scale (VAS) scores for knee pain intensity of 68.00 ±â€¯9.58 (UT group) and 71.00 ±â€¯8.74 (PP group, respectively) before treatment, were randomly allocated into two groups. Both groups were treated with an ultrasound program in continuous mode, 1.0 W/cm2, 10 min per session, for 10 sessions. Nanoparticles of P. amarus were used in the PP group, whereas a nondrug coupling gel was used in the UT group. The 6-min walk test (6-MWT) was performed to evaluate functional capacity. The VAS and the 6-MWT were evaluated before and after 10 treatment sessions in both groups using a double-blind procedure. RESULTS: VAS and 6-MWT showed significant improvement after treatment in both groups (p < 0.05). The PP group showed more significant effects than the UT group, in terms of both reducing the VAS pain score (p < 0.05) and improving 6-MWT (p < 0.05). CONCLUSIONS: PP is suggested as an effective method for the treatment of symptomatic knee OA for reducing pain and improving functional capacity.


Assuntos
Osteoartrite do Joelho/terapia , Fonoforese/métodos , Phyllanthus , Terapia por Ultrassom/métodos , Administração Cutânea , Idoso , Terapia Combinada , Método Duplo-Cego , Feminino , Humanos , Articulação do Joelho/fisiopatologia , Masculino , Pessoa de Meia-Idade , Medição da Dor , Creme para a Pele/uso terapêutico , Fatores de Tempo , Resultado do Tratamento
7.
Ultrasound Med Biol ; 45(9): 2575-2582, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31262523

RESUMO

Controlling microbubble-mediated drug delivery requires the underlying biological and physical mechanisms to be unraveled. To image both microbubble oscillation upon ultrasound insonification and the resulting cellular response, we developed an optical imaging system that can achieve the necessary nanosecond temporal and nanometer spatial resolutions. We coupled the Brandaris 128 ultra-high-speed camera (up to 25 million frames per second) to a custom-built Nikon A1R+ confocal microscope. The unique capabilities of this combined system are demonstrated with three experiments showing microbubble oscillation leading to either endothelial drug delivery, bacterial biofilm disruption, or structural changes in the microbubble coating. In conclusion, using this state-of-the-art optical imaging system, microbubble-mediated drug delivery can be studied with high temporal resolution to resolve microbubble oscillation and high spatial resolution and detector sensitivity to discern cellular response. Combining these two imaging technologies will substantially advance our knowledge on microbubble behavior and its role in drug delivery.


Assuntos
Sistemas de Liberação de Medicamentos , Microbolhas , Microscopia Confocal , Imagem Óptica/métodos , Fonoforese/métodos , Desenho de Equipamento , Células Endoteliais da Veia Umbilical Humana , Humanos
8.
Am J Phys Med Rehabil ; 97(5): 332-338, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29206666

RESUMO

OBJECTIVE: The aim of the study was to investigate the efficacy of phonophoresis with combined therapy on active myofascial trigger points. PARTICIPANTS: One hundred participants with acute mechanical neck pain and at least one active myofascial trigger point in the upper trapezius were randomly assigned into four equal groups. INTERVENTION: Groups consisted of diclofenac phonophoresis with combined therapy, diclofenac phonophoresis, ultrasound (US) with coupling gel, and sham US and applied for 10 mins over myofascial trigger points. MEASUREMENTS: Measurements included pressure pain threshold and active cervical lateral flexion. RESULTS: There were statistically significant improvements in postintervention pressure pain threshold and range of motion values in treatment groups (P < 0.0001). As for the sham US, no significant difference was found between the preintervention and postintervention values (P > 0.05). Bonferroni correction test revealed that there was a significant difference between all the four groups in pressure pain threshold values (P < 0.0001); however, it was nonsignificant (P > 0.05) for range of motion. CONCLUSIONS: Diclofenac phonophoresis with combined therapy, phonophoresis, and US were all effective in increasing pressure pain threshold values and range of motion. In addition, phonophoresis with combined therapy was shown to be superior over phonophoresis, and phonophoresis was superior over US in terms of reducing pain sensitivity. However, none of the treatment groups were found to be superior over the other in increasing range of motion.


Assuntos
Síndromes da Dor Miofascial/terapia , Fonoforese/métodos , Estimulação Elétrica Nervosa Transcutânea/métodos , Adulto , Anti-Inflamatórios não Esteroides/administração & dosagem , Terapia Combinada , Diclofenaco/administração & dosagem , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Síndromes da Dor Miofascial/fisiopatologia , Medição da Dor , Limiar da Dor , Amplitude de Movimento Articular , Método Simples-Cego , Músculos Superficiais do Dorso/fisiopatologia , Resultado do Tratamento
9.
JNMA J Nepal Med Assoc ; 56(207): 357-61, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29255320

RESUMO

INTRODUCTION: Acute calcific tendinitis of shoulder is very painful and disturbing condition. There are many modalities with variable outcome to address the condition. We studied needling technique with multiple fenestration, subacromial steroid and diclofenac phonophoresis and evaluated the outcome in term of pain relief and improvement of shoulder function at a simple outpatient clinical set up without image or ultrasound guidance. METHODS: We studied cases of acute calcific tendinitis presenting within one week from onset of symptoms and X-ray showing calcific deposit. All underwent needle fenestration at maximum tender site with lignocaine loaded syringe, subsequent injection of 40 mg subacromial methylprednisolone and diclofenac phonophoresis done by qualified physiotherapist for five days. Visual Analogue Scale was used to measure pain, Simple shoulder Test applied to evaluate shoulder function and size of calcific deposit was measured at maximum length. RESULTS: Pain subsided dramatically and there was substantial improvement of shoulder function within a week. CONCLUSIONS: Needle fenestration and subacromial methylprednisolone along with diclofenac phonophoresis without image guidance gives excellent pain reduction and improves shoulder function which can be done at simple outpatient clinic.


Assuntos
Anestésicos Locais/uso terapêutico , Anti-Inflamatórios não Esteroides/uso terapêutico , Calcinose/terapia , Diclofenaco/uso terapêutico , Glucocorticoides/uso terapêutico , Lidocaína/uso terapêutico , Metilprednisolona/uso terapêutico , Fonoforese/métodos , Punções/métodos , Lesões do Manguito Rotador/terapia , Doença Aguda , Administração Tópica , Adulto , Idoso , Feminino , Humanos , Injeções Intra-Articulares , Masculino , Pessoa de Meia-Idade , Agulhas , Medição da Dor
10.
Technol Health Care ; 25(6): 1131-1138, 2017 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-28946604

RESUMO

A needle-free vaccine/drug injector that works by virtue of the impulse of a moving shock wave is presented in this communication. The device can deliver controlled micro-volumes of liquid vaccines into skin and soft tissue targets in human with minimal invasion. The operation of the injector was investigated by delivering a dyed liquid into human skin samples and soft tissue models. The depth of penetration of the liquid was examined by histology of the targeted human skin samples. The delivery mechanics and the depth of penetration were analyzed theoretically with an elastic model for the skin and a viscoelastic model for the soft tissue targets, and a good agreement with experiments was observed. The current liquid vaccine/drug delivery technique can reduce pain, trauma and contamination, and can offer a cost-effective, needle-free, health-care solution.


Assuntos
Sistemas de Liberação de Medicamentos/métodos , Ondas de Choque de Alta Energia , Fonoforese/métodos , Pele/metabolismo , Vacinas/administração & dosagem , Desenho de Equipamento , Humanos
11.
Artigo em Russo | MEDLINE | ID: mdl-28884739

RESUMO

The present study was designed to investigate kinetics of flavonoidrelease from the working compositions containing a phytocomplex. The basic parameters of this processes during phonophoresis were determined in the model in vitro experiments. The study has demonstrated the dependence of the flavonoid release rate on their initial concentration in the working compositions and the influence of dimethylsulfoxide (as well as the main and auxiliary agents of the working composition) on the release of biologically active substances. The technological methods designed for the enhancement of the effectiveness of the phytocomplex phonophoresis technique are proposed.


Assuntos
Flavonoides/farmacocinética , Modelos Biológicos , Fonoforese/métodos , Preparações de Plantas/administração & dosagem , Dimetil Sulfóxido/farmacocinética , Liberação Controlada de Fármacos , Humanos , Técnicas In Vitro , Preparações de Plantas/farmacocinética , Fatores de Tempo
12.
J Bodyw Mov Ther ; 21(3): 743-746, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28750995

RESUMO

Pain in the coccyx is referred as coccydynia. The pain aggravates in weight bearing i.e. sitting. Total 48 persons with coccydynia diagnosed clinically were recruited and randomly assigned into one of the 3 groups. Experimental group I were treated by stretching of piriformis and iliopsoas muscles, experimental group II were treated by stretching of piriformis and iliopsoas muscles and Maitland's rhythmic oscillatory thoracic mobilization over the hypomobile segments and the conventional group were treated by seat cushioning + Sitz bath + Phonophorosis. All participants underwent an initial baseline assessment for Pressure Pain Threshold (PPT) by using modified syringe algometer and pain free sitting duration. All the subjects were advised to minimise sitting posture and use a seat cushion. Treatment was given for 3 weeks, 5 sessions per week and post-treatment evaluation was done after completion of 3 weeks. Follow up evaluation was done after 1 month. DATA ANALYSIS: The data was analyzed by using 3 × 3 ANOVA. Tukey's HSD post-Hoc analysis was used for all pair wise comparison. RESULTS: The overall results of the study showed that there was significant improvement in pain pressure threshold and pain free sitting in both the experimental groups with treatment and improvement continued after cessation of therapy, whereas the conventional group did not improve significantly.


Assuntos
Cóccix , Dor Lombar/terapia , Músculo Esquelético/fisiologia , Manipulações Musculoesqueléticas/métodos , Terapia Combinada , Feminino , Humanos , Masculino , Limiar da Dor/fisiologia , Fonoforese/métodos , Postura , Pressão , Músculos Psoas/fisiologia
13.
Technol Health Care ; 25(S1): 305-315, 2017 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-28582919

RESUMO

BACKGROUND: Due to different physical and biological mechanisms behind ultrasound hyperthermia and phonophoresis, the requirement for ultrasound power, frequency and control modes varies. OBJECTIVE: This paper introduces an adaptive ultrasonic physiotherapy system based on real-time surveillance over physiological characteristics of the patients, which in turn assists the individual treatment and dose limitation in auxiliary rehabilitation. METHODS: The method essentially takes advantage of distinctive characteristics of two different phases (systole and diastole) of the human cardiac cycle as a medium for modulation. The abundance of blood flow during systole enables energy exchange for hyperthermia while blood flow insufficiency caused by diastole assists in drug penetration. Said method could improve the adjuvant therapy as it provides partial drug penetration and therapeutic dosage control. RESULTS: By adjusting time window and intensity of multi-frequency ultrasound, it is possible to reduce the irradiation dosage to around 22% of that during continuous irradiation at 1 MHz. The method shows high potential in clinical practice. CONCLUSION: Frequency-tuning ultrasound therapy would be more efficient regarding drug penetration and improve the therapeutic efficacy of hyperthermia.


Assuntos
Modalidades de Fisioterapia/instrumentação , Terapia por Ultrassom/métodos , Pressão Sanguínea/fisiologia , Pressão Sanguínea/efeitos da radiação , Volume Sanguíneo/fisiologia , Diástole/fisiologia , Diástole/efeitos da radiação , Desenho de Equipamento , Retroalimentação/efeitos da radiação , Humanos , Hipertermia Induzida/instrumentação , Hipertermia Induzida/métodos , Modelos Estatísticos , Farmacocinética , Fonoforese/instrumentação , Fonoforese/métodos , Sístole/fisiologia , Sístole/efeitos da radiação , Terapia por Ultrassom/instrumentação , Ondas Ultrassônicas
14.
Vestn Otorinolaringol ; 81(4): 22-26, 2016.
Artigo em Russo | MEDLINE | ID: mdl-27500573

RESUMO

The objective of the present study was to develop an efficient system for the treatment of chronic tonsillitis in the patients of advanced and middle age based on the application of polyvalent bacteriophages in the combination with the physical factors and herbal medicines. The study involved 65 patients (39 women and 276 men) at the age from 65 to 73 years presenting with chronic tonsillitis. The treatment included washing the tonsillar lacunae with herbal infusion consisting of a tetterwort (Choledoniummajus) extract. This procedure was followed by phonophoresiswith the use of the combined polyvalent bacteriophage preparation in the non-liquid formulation during 7-10 days. The effectiveness of such treatment was evaluated based on the results of clinical examination and the analysis of the subjective feelings reported by the patients. In addition, the rosette-forming function of lymphocytes was estimated and palatine tonsil microbiotas in different patients were compared. The effectiveness of therapy was estimated at 89.2%. The positive outcome of the proposed treatment was documented in 78.6% of the cases within 6 months after the onset of therapy. It is concluded that the treatment of chronic tonsillitis with bacteriophagal preparations and herbal infusions in combination with thetraditionallow-frequency ultrasound treatment is highly efficacious (favourable outcome in 78.6% of the patients of middle and advanced age) without the use of antibiotic medications.


Assuntos
Bacteriófagos , Chelidonium , Microbiota , Fonoforese/métodos , Fitoterapia/métodos , Extratos Vegetais/administração & dosagem , Tonsilite , Idoso , Tratamento Conservador/métodos , Feminino , Humanos , Masculino , Microbiota/efeitos dos fármacos , Microbiota/fisiologia , Preferência do Paciente , Irrigação Terapêutica/métodos , Tonsilite/diagnóstico , Tonsilite/microbiologia , Tonsilite/fisiopatologia , Tonsilite/terapia , Resultado do Tratamento
15.
J Drug Target ; 24(8): 703-8, 2016 09.
Artigo em Inglês | MEDLINE | ID: mdl-26811100

RESUMO

BACKGROUND: Synthesized dl-Nordihydroguaiaretic acid (dl-NGDA or "Nordy") can inhibit the growth of malignant human tumors, especially the tumor angiogenesis. However, its liposoluble nature limits its in vivo efficacy in the hydrosoluble circulation of human. PURPOSE: We tried to use the ultrasonic microbubble as the carrier and the ultrasound-induced destruction for the targeted release of Nordy and evaluate its in vitro and in vivo anti-tumor effect. METHODS: Nordy-loaded lipid microbubbles were prepared by mechanical vibration. Effects of ultrasound-induced Nordy-loaded microbubbles destruction on proliferation of human umbilical vein endothelial cells (HUVECs), tumor derived endothelial cells (Td-ECs), and rabbit transplanted VX2 tumor models were evaluated. RESULTS: The ultrasound-induced Nordy-loaded microbubbles destruction inhibited the proliferations of HUVECs and Td-ECs in vitro, and inhibited the tumor growth and the microvasculature in vivo. Its efficacy was higher than those of Nordy used only and Nordy with ultrasound exposure. CONCLUSION: Ultrasonic microbubbles can be used as the carrier of Nordy and achieve its targeted release with improved anti-tumor efficacy in the condition of ultrasound-induced microbubbles destruction.


Assuntos
Inibidores da Angiogênese/administração & dosagem , Sistemas de Liberação de Medicamentos/métodos , Masoprocol/administração & dosagem , Microbolhas , Fonoforese/métodos , Ondas Ultrassônicas , Inibidores da Angiogênese/uso terapêutico , Animais , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Células Endoteliais da Veia Umbilical Humana , Humanos , Masoprocol/uso terapêutico , Neoplasias Experimentais/irrigação sanguínea , Neoplasias Experimentais/tratamento farmacológico , Coelhos
16.
J Cosmet Laser Ther ; 18(2): 116-23, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26821226

RESUMO

BACKGROUND: The topical use of caffeine has been indicated for the lipodystrophies treatment as it promotes increased lipolysis. Ultrasound (US) is often used in cutaneous diseases, esthetic conditions, and as a skin permeation enhancer. OBJECTIVE: We investigate the lipolytic response of adipocytes isolated from subcutaneous adipose pigs tissue subjected to treatment with topical application of phonophoresis associated with caffeine. METHOD: We treated dorsal regions of pigs (Landrace × Large White, 35 days, 15 kg, n = 6) daily for 15 days with gel, gel + US [3 MHz, continuous, 0.2 Wcm(2), 1 min/cm(2), in total 2 min], gel + caffeine (5%w/w), and gel + caffeine + US. We used a fifth untreated region as control. Twenty-four hours after the last application, we isolated the adipocytes of each treated area and quantified the basal and stimulated lipolytic responses to isoprenaline. The results, in µmol glycerol/10(6)cells/60 min, were analyzed with analysis of variance or ANOVA followed by Newman-Keuls test. The value of p < 0.05 was indicative of statistical difference. RESULTS: Only the adipocytes isolated from the area treated with caffeine + US showed increased basal lipolysis (0.76 ± 0.26; p = 0.0276) and maximal isoprenaline stimulation (0.38 ± 0.15, p = 0.0029) compared with the other areas. CONCLUSION: The results demonstrate that increased lipolysis of caffeine + US is due to an increase in basal and beta-adrenoceptor response by caffeine, and caffeine's effect is local, avoiding unwanted effects.


Assuntos
Adipócitos/efeitos dos fármacos , Cafeína/administração & dosagem , Cafeína/farmacologia , Lipólise/efeitos dos fármacos , Fonoforese/métodos , Animais , Masculino , Gordura Subcutânea , Suínos
17.
Drug Deliv ; 23(5): 1588-93, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26669820

RESUMO

Electro-phonophoresis (EP) has been used in various clinical fields. The objective of present study is to evaluate the skin permeability of isoniazid (INH) and rifampicin (RIF) in patients with tuberculous lymphadenitis with the aid of EP to validate the clinical applications of this transdermal delivery system for the treatment of superficial extrapulmonary tuberculosis. INH and RIF solutions were delivered transdermally, with or without EP, in the surrounding tissue of the lesion for 0.5 h. Local pyogenic fluids or necrotic tissue samples from the infection sites in patients were collected at 1 h after dosing. Drug concentrations in samples were evaluated by high performance liquid chromatography. The median INH and RIF intra-lesional concentrations were 0.365 (interquartile range [IQR] 0.185-1.775) µg/mL and 1.231 (IQR 0.304-1.836) µg/mL in oral group; 2.964 (IQR 0.193-7.325) µg/mL and 2.646 (IQR 1.211-3.753) µg/mL in INH- and RIF-transdermal plus EP group. Drug concentrations in the local sites of patients receiving INH or RIF through EP transdermal delivery were statistically higher than those observed in patients only taking INH and RIF orally. However, this enhancement was not observed in the transdermal delivery of INH or RIF without EP in contrast to the oral administrations of drugs. EP can effectively enhance the skin permeability of INH and RIF in patients with tuberculous lymphadenitis. The increase in drug concentrations in the lesions could help eradication of the germs; shorten the treatment course and increase the cure rate of patients with tuberculous lymphadenitis.


Assuntos
Antituberculosos/administração & dosagem , Antituberculosos/farmacologia , Isoniazida/administração & dosagem , Isoniazida/farmacologia , Linfadenite/tratamento farmacológico , Mycobacterium tuberculosis/efeitos dos fármacos , Fonoforese/métodos , Rifampina/administração & dosagem , Rifampina/farmacologia , Administração Cutânea , Antituberculosos/química , Feminino , Humanos , Isoniazida/química , Linfadenite/patologia , Masculino , Mycobacterium tuberculosis/química , Permeabilidade
18.
Physiotherapy ; 102(1): 57-63, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26051846

RESUMO

OBJECTIVE: To determine whether dexketoprofen administered by phonophoresis or iontophoresis is more effective for the treatment of subacromial impingement syndrome (SIS) than conventional ultrasound therapy. DESIGN: Randomised clinical trial. SETTING: University hospital. PARTICIPANTS: Ninety-nine participants with SIS without a complete tear of the rotator cuff were assigned at random to three intervention groups. INTERVENTION GROUPS: Participants received ultrasound (n=32), phonophoresis with dexketoprofen (50mg/session) (n=33) or iontophoresis with dexketoprofen (50mg/session) (n=34). All participants completed 20 treatment sessions plus exercise therapy and cryotherapy. OUTCOME MEASURES: A visual analogue scale (VAS), the Constant-Murley Scale (CMS) and the Disabilities of the Arm, Shoulder and Hand (DASH) questionnaire were administered pre-treatment (baseline), post-treatment and 1 month post-treatment. RESULTS: At baseline, there were no differences between the groups. Post-treatment, VAS score improved by -1.2 points and CMS score improved by 8.9 points in the ultrasound group compared with the iontophoresis group [95% confidence interval (CI) -0.2 to -2.2 and 95% CI 17.0 to 0.7, respectively]. CMS score improved by 7.1 points in the phonophoresis group compared with the iontophoresis group (95% CI 14.8 to -0.7). At 1 month post-treatment, no significant differences were detected between the groups. VAS, CMS and DASH scores of all groups improved post-treatment and at 1 month post-treatment. CONCLUSION: Ultrasound, iontophoresis with dexketoprofen and phonophoresis with dexketoprofen can improve pain, shoulder function, and physical functioning and symptoms in the upper limb in patients with SIS without a complete tear of the rotator cuff. CLINICAL TRIALS. GOV REGISTRATION NUMBER: NCT01748188.


Assuntos
Anti-Inflamatórios não Esteroides/uso terapêutico , Iontoforese/métodos , Cetoprofeno/análogos & derivados , Fonoforese/métodos , Síndrome de Colisão do Ombro/terapia , Trometamina/uso terapêutico , Terapia por Ultrassom/métodos , Adulto , Idoso , Anti-Inflamatórios não Esteroides/administração & dosagem , Crioterapia/métodos , Terapia por Exercício , Feminino , Hospitais Universitários , Humanos , Cetoprofeno/administração & dosagem , Cetoprofeno/uso terapêutico , Masculino , Pessoa de Meia-Idade , Medição da Dor , Amplitude de Movimento Articular , Dor de Ombro/terapia , Método Simples-Cego , Trometamina/administração & dosagem
19.
Drug Deliv ; 23(3): 926-32, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-24937380

RESUMO

The aim of this study was to evaluate the effects of therapeutic pulsed ultrasound with gold nanoparticles on oxidative stress parameters after traumatic muscle injury in Wistar rats. The animals were randomly divided into nine groups (n = 6 each): sham (uninjured muscle); muscle injury without treatment; muscle injury and treatment with dimethyl sulfoxide (15 mg/kg); muscle injury and treatment with gold nanoparticles (27 µg); muscle injury and treatment with dimethyl sulfoxide + gold nanoparticles (Plus); muscle injury and therapeutic pulsed ultrasound; muscle injury and therapeutic pulsed ultrasound + dimethyl sulfoxide; muscle injury and therapeutic pulsed ultrasound + gold nanoparticles; and muscle injury and therapeutic pulsed ultrasound + Plus. Gastrocnemius injury was induced by a single-impact blunt trauma. Therapeutic pulsed ultrasound (6-min application, frequency 1.0 MHz, intensity 0.8 W/cm(2)) was used 2, 12, 24, and 48 h after trauma. Mitochondrial superoxide generation, lipid peroxidation, and protein carbonylation, and the activities of superoxide dismutase, glutathione peroxidase, and catalase were evaluated. The increase in the superoxide production and TBARS and carbonyl levels observed in the control group after muscle damage were reduced in animals exposed to therapeutic pulsed ultrasound plus nanoparticles. Similarly, antioxidants enzymes showed a decreased activity with the same treatment. Our work suggest that therapeutic pulsed ultrasound + dimethyl sulfoxide + gold nanoparticles has beneficial effects on the muscle healing process by inducing a decrease in oxidative stress parameters and most likely decreasing the deleterious effects of the inflammatory response.


Assuntos
Ouro/farmacologia , Nanopartículas Metálicas/administração & dosagem , Músculo Esquelético/efeitos dos fármacos , Doenças Musculares/tratamento farmacológico , Estresse Oxidativo/efeitos dos fármacos , Ferimentos e Lesões/tratamento farmacológico , Animais , Antioxidantes/metabolismo , Catalase/metabolismo , Dimetil Sulfóxido/metabolismo , Modelos Animais de Doenças , Glutationa Peroxidase/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Músculo Esquelético/metabolismo , Doenças Musculares/metabolismo , Fonoforese/métodos , Ratos , Ratos Wistar , Superóxido Dismutase/metabolismo , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo , Ferimentos e Lesões/metabolismo
20.
Cancer Res ; 75(18): 3738-46, 2015 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-26216548

RESUMO

Pancreatic cancer is characterized by extensive stromal desmoplasia, which decreases blood perfusion and impedes chemotherapy delivery. Breaking the stromal barrier could both increase perfusion and permeabilize the tumor, enhancing chemotherapy penetration. Mechanical disruption of the stroma can be achieved using ultrasound-induced bubble activity-cavitation. Cavitation is also known to result in microstreaming and could have the added benefit of actively enhancing diffusion into the tumors. Here, we report the ability to enhance chemotherapeutic drug doxorubicin penetration using ultrasound-induced cavitation in a genetically engineered mouse model (KPC mouse) of pancreatic ductal adenocarcinoma. To induce localized inertial cavitation in pancreatic tumors, pulsed high-intensity focused ultrasound (pHIFU) was used either during or before doxorubicin administration to elucidate the mechanisms of enhanced drug delivery (active vs. passive drug diffusion). For both types, the pHIFU exposures that were associated with high cavitation activity resulted in disruption of the highly fibrotic stromal matrix and enhanced the normalized doxorubicin concentration by up to 4.5-fold compared with controls. Furthermore, normalized doxorubicin concentration was associated with the cavitation metrics (P < 0.01), indicating that high and sustained cavitation results in increased chemotherapy penetration. No significant difference between the outcomes of the two types, that is, doxorubicin infusion during or after pHIFU treatment, was observed, suggesting that passive diffusion into previously permeabilized tissue is the major mechanism for the increase in drug concentration. Together, the data indicate that pHIFU treatment of pancreatic tumors when resulting in high and sustained cavitation can efficiently enhance chemotherapy delivery to pancreatic tumors. .


Assuntos
Antibióticos Antineoplásicos/administração & dosagem , Carcinoma Ductal Pancreático/tratamento farmacológico , Doxorrubicina/administração & dosagem , Sistemas de Liberação de Medicamentos/métodos , Ondas de Choque de Alta Energia , Neoplasias Pancreáticas/tratamento farmacológico , Fonoforese/métodos , Animais , Antibióticos Antineoplásicos/farmacocinética , Carcinoma Ductal Pancreático/genética , Carcinoma Ductal Pancreático/patologia , Difusão , Doxorrubicina/farmacocinética , Sistemas de Liberação de Medicamentos/instrumentação , Desenho de Equipamento , Camundongos , Camundongos Transgênicos , Microscopia de Fluorescência , Neoplasias Pancreáticas/genética , Neoplasias Pancreáticas/patologia , Fonoforese/instrumentação , Distribuição Aleatória , Células Estromais/patologia , Espectrometria de Massas em Tandem
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