RESUMO
Physical and biochemical changes in the spinal cord of monkeys at 1/2, 2, and 4 hours following 200 g cm contusion injury and 50 g of compression injury and 2 hours of decompression following 4 hours of compression were studied. The pathophysiologic changes were milder in compression compared to contusion injury. Following contusion injury, at 1/2 and 2 hours there was significant increase in % water content, lipid peroxidation, and alpha-L-fucosidase. alpha-D-Mannosidase was significantly increased at all time periods, and beta-D-hexosaminidase was increased at 1/2 and 4 hours. At 4 hours following injury, serotonin (5 HT) and 5-hydroxyindole-3-acetic acid (5-HIAA) showed a significant increase. From 10 minutes to 2 hours there was increased platelet aggregation. In compression injury, a significant increase in water content and 5 HT was observed only at 1/2 hour. Lipid peroxidation had increased at all time periods, whereas B-D-hexosaminidase, beta-D-galactosidase, and 5-HIAA were increased at 2 hours. alpha-D-Mannosidase had increased at 1/2 and 2 hours, and alpha-L-fucosidase had increased at 4 hours. After 2 hours decompression following 4 hours compression, water content, beta-D-galactosidase, and alpha-D-Mannosidase were significantly increased. An attempt was made to correlate the findings and to understand the sequential pathophysiologic changes in the first 4 hours following spinal cord trauma, providing a baseline for evaluation of the efficacy of any therapeutic maneuvers.
Assuntos
Contusões/metabolismo , Edema/etiologia , Peróxidos Lipídicos/metabolismo , Lisossomos/enzimologia , Serotonina/metabolismo , Compressão da Medula Espinal/metabolismo , Doenças da Medula Espinal/etiologia , Traumatismos da Medula Espinal/metabolismo , Doença Aguda , Animais , Contusões/fisiopatologia , Feminino , Galactosidases/líquido cefalorraquidiano , Hexosaminidases/líquido cefalorraquidiano , Ácido Hidroxi-Indolacético/metabolismo , Macaca radiata , Masculino , Manosidases/líquido cefalorraquidiano , Agregação Plaquetária , Medula Espinal/metabolismo , Compressão da Medula Espinal/fisiopatologia , Traumatismos da Medula Espinal/fisiopatologia , Fatores de Tempo , alfa-L-Fucosidase/líquido cefalorraquidianoRESUMO
The activities of acid phosphatase and N-acetyl-beta-glucosaminidase have been measured in 171 samples of cerebrospinal fluid from 104 patients suffering from multiple sclerosis. The mean level of activity of these enzymes was lower than of controls. Patients who had the first or second bouts had somewhat higher activity of these enzymes compared to controls. The lowest values of these enzymes were found in patients with severe disability. Patients with late onset of the disease had higher levels of the enzymes compared to patients with an earlier debut of the illness, whereas patients with a short history had higher values than patients with a longer duration.
Assuntos
Acetilglucosaminidase/líquido cefalorraquidiano , Fosfatase Ácida/líquido cefalorraquidiano , Galactosidases/líquido cefalorraquidiano , Hexosaminidases/líquido cefalorraquidiano , Manosidases/líquido cefalorraquidiano , Esclerose Múltipla/enzimologia , beta-Galactosidase/líquido cefalorraquidiano , Adolescente , Adulto , Idoso , Feminino , Humanos , Lisossomos/enzimologia , Masculino , Pessoa de Meia-Idade , Esclerose Múltipla/líquido cefalorraquidianoRESUMO
In seven patients with cerebral atrophy due to pre-senile dementia and/or cerebrovascular disease, the activity of acid phosphatase in lumbar cerebrospinal fluid (CSF) was higher (p less than 0.05) than in six controls. The activity of arylsulphatase and beta-galactosidase in CSF was the same in the two groups. In the serum, the activities of acid phosphatase and arylsulphatase were the same in the two groups but the activity of beta-galactosidase was lower (p less than 0.02) in patients with cerebral atrophy.
