RESUMO
Antiserum to 1 alpha-hydroxycorticosterone binding protein was used to investigate its location in selected tissues of the skate Raja ocellata. Immunofluorescence, using an indirect technique, showed 1 alpha-hydroxycorticosterone binding sites in potential target tissue: gill. chloride cells, rectal gland parenchyma, and a portion of the kidney tubule. The binding protein was not detectable in the ventricle or the intestinal spiral valve but was associated with liver parenchyma and interrenal cells. The intracellular location of the binding protein is apparently tissue specific.
Assuntos
Proteínas de Transporte/análise , Corticosterona/análogos & derivados , Peixe Elétrico/metabolismo , Rajidae/metabolismo , Animais , Corticosterona/metabolismo , Citoplasma/análise , Glândulas Exócrinas/análise , Imunofluorescência , Brânquias/análise , Glândula Inter-Renal/análise , Rim/análise , Fígado/análise , Masculino , Glândula de Sal/química , Distribuição TecidualAssuntos
Glândulas Suprarrenais/análise , Aldosterona/análise , Glândula Inter-Renal/análise , Hormônio Adrenocorticotrópico/farmacologia , Aldosterona/metabolismo , Animais , Células Cultivadas , Reações Cruzadas , Soros Imunes , Glândula Inter-Renal/efeitos dos fármacos , Glândula Inter-Renal/metabolismo , Microquímica , Perfusão , Fenetilaminas/farmacologia , Coelhos/imunologia , Radioimunoensaio/métodos , RanidaeRESUMO
Partial hepatectomy was carried out on Xenopus laevis to investigate its influence on the endocrine system. In addition to other endocrine effects, a marked hypertrophy and stimulation of the interrenal gland was observed. Activated cells contain mitochondria with extended and irregularly coiled tubules embedded in a low electron dense matrix. Hepatectomy induces two phases of proliferation [3 and 35 days postoperative (p.o.)]. After 106 days p.o. giant mitochondria possessing narrow and closely packed, parallel tubules surrounded by an electron dense matrix indicate a phase of inactivation. The smooth endoplasmic reticulum and the Golgi apparatus proliferate after hepatectomy. During activation the high lipid content seen in controls in decreased significantly.
