Assuntos
Herpesvirus Bovino 2/enzimologia , Herpesvirus Bovino 2/patogenicidade , Ribonucleotídeo Redutases/metabolismo , Sequência de Aminoácidos , Animais , Bovinos , Doenças dos Bovinos/imunologia , Doenças dos Bovinos/prevenção & controle , Genes Virais , Herpes Simples/imunologia , Herpes Simples/prevenção & controle , Herpes Simples/veterinária , Herpesvirus Bovino 2/genética , Herpesvirus Bovino 2/imunologia , Dados de Sequência Molecular , Ribonucleotídeo Redutases/genética , Timidina Quinase/genética , Timidina Quinase/metabolismo , Vacinas Virais/genética , Vacinas Virais/imunologia , Virulência/genética , Virulência/imunologia , Virulência/fisiologiaRESUMO
Bovine herpesvirus type 2 (BoHV-2), also known as bovine mammillitis virus, is classified in the Family Herpesviridae, Subfamily Alphaherpesvirinae, and Genus Simplexvirus along with herpes simplex viruses type 1 and 2 (HSV-1 and HSV-2) and other primate simplexviruses on the basis of similarities in 4 genes within the 15 kb U(L) 23-29 cluster. This could be explained either by a global similarity or a recombination event that brought primate herpesviral sequences into a bovine virus. Our sequences for DNA polymerase (U(L)30), a large gene adjacent to the previously identified conserved cluster, and glycoprotein G (U(S)4), a gene as distant from the cluster as possible on the circularized genome, confirm the close relationship between BoHV-2 and the primate simplexviruses, and argue for a global similarity and probably a close evolutionary relationship. Thus one can speculate that BoHV-2 may represent a greater hazard to humans than has been appreciated previously.
Assuntos
Alphaherpesvirinae/genética , Herpesvirus Bovino 2/genética , Primatas/virologia , Alphaherpesvirinae/classificação , Alphaherpesvirinae/enzimologia , Sequência de Aminoácidos , Animais , Bovinos , Linhagem Celular , DNA Viral/análise , DNA Polimerase Dirigida por DNA/genética , Herpesvirus Bovino 2/classificação , Herpesvirus Bovino 2/enzimologia , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase , Homologia de Sequência de AminoácidosRESUMO
The nucleotide (nt) sequence of the thymidine kinase (TK) gene (a 918 nt long coding region) of two TK-deficient (TK) strains of bovine herpesvirus 2 (BHV-2) was determined. The candidate vaccine strain C290BU5, which was no longer able to cause disease, was found to have an A deletion after nt 887 of the TK gene with a predicted change of His 296 to Pro, altering the last 10 amino acids (aa) and extending the gene by another 34 aa. The strain which still caused disease, C290BU3, had a T insertion after nt 16 causing a predicted chain termination after only 16 aa.
Assuntos
Herpesvirus Bovino 2/enzimologia , Timidina Quinase/genética , Vacinas Virais/genética , DNA Viral/genética , Genes Virais , Herpesvirus Bovino 2/genética , Mutação Puntual , Deleção de Sequência , Timidina Quinase/metabolismo , Proteínas Estruturais Virais/genéticaAssuntos
Doenças dos Bovinos/etiologia , Herpes Simples/veterinária , Herpesvirus Bovino 2/isolamento & purificação , Timidina Quinase/deficiência , Animais , Sequência de Bases , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/prevenção & controle , DNA Viral/análise , DNA Viral/química , DNA Viral/genética , Cobaias , Herpes Simples/epidemiologia , Herpes Simples/etiologia , Herpesvirus Bovino 2/enzimologia , Herpesvirus Bovino 2/genética , Reação em Cadeia da Polimerase/veterinária , Timidina Quinase/genética , Vacinas/imunologia , Vitória/epidemiologiaRESUMO
The precise genomic location and the nucleotide sequence of the bovine herpesvirus type 2 (bovine herpes mammillitis virus) thymidine kinase (TK) gene have been determined. The genomic location of the TK gene was found to be in a similar position to that of herpes simplex virus. The coding region consists of 918 bases, which is slightly smaller in length than other reported herpesvirus TK genes. However with an Mr of 38,108 the individual protein is similar in size to other herpesvirus TK enzymes. Despite there being only limited overall sequence homology with the TK genes of other herpesviruses, there are several regions of extensive homology at the amino acid level.
