Assuntos
Doenças dos Bovinos/prevenção & controle , Herpes Simples/veterinária , Herpesvirus Bovino 2/metabolismo , Timidina Quinase/análise , Vacinas Virais/administração & dosagem , Animais , Bovinos , Cobaias , Herpesvirus Bovino 2/imunologia , Herpesvirus Bovino 2/patogenicidade , Vacinas Virais/química , VirulênciaRESUMO
Polypeptides synthesized in bovine testes cells infected with bovine herpesvirus type 2 were labeled with [35S]methionine and were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Thirty-eight virus-induced proteins, ranging in molecular weight from 32,000 to 149,000, were detectable by analysis of whole cell lysates between postinfection hours 2 and 36. A similar number was immunoprecipitated by rabbit antiserum to bovine herpesvirus type 2. Twelve proteins incorporated [3H]glucosamine. On the basis of their temporal characteristics and their pattern of synthesis in the presence of cycloheximide and dactinomycin, 3 proteins, including at least one that was nondetectable in the absence of drugs, were classified as alpha proteins, 4 as beta proteins, 1 as a beta/gamma protein, and 27 as gamma proteins. Host cell protein synthesis was not reduced substantially until postinfection hours 22 to 28.
