RESUMO
A high-pressure liquid chromatographic method was developed to quantify 2,4-disulfamyl-5-trifluoromethylaniline in hydroflumethiazide using a bonded pellicular packing. An internal standard was added to the samples to ensure reproducibility. The results were compared with methods currently in use.
Assuntos
Compostos de Anilina/análise , Hidroflumetiazida/análise , Compostos de Anilina/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Métodos , SulfonamidasRESUMO
A spectrofluorometric method for determination of hydroflumethiazide in human plasma and urine has been developed. The limit of detection was 10 ng/ml plasma and 100 ng/ml urine. The plasma concentration of hydroflumethiazide was determined for 9-11 hours and excretion in urine for 24-37 hrs after oral administration of about 1 mg/kg body weight to 7 subjects. Plasma half life in healthy subjects was 1.9-2.1 h, and 2.7-8.6 h in patients during the period 4-9 hrs after dosing. Cumulative excretion in urine was 67-79% of the dose during 31-37 hrs in 6 subjects; one patient with renal disease was found to excrete only 25.8% of dose during 24 hours. Renal clearance of hydroflumethiazide was higher in the healthy subjects (0.29-0.44 1 h-1 kg-1) than in the patients (0.040-0.15 1 h-1 kg-1). Plasma half life of hydroflumethiazide was not closely correlated with renal clearance of the drug, which suggests that other factors may play a role in determining plasma half life.
Assuntos
Hidroflumetiazida/análise , Administração Oral , Adulto , Idoso , Peso Corporal , Feminino , Meia-Vida , Humanos , Hidroflumetiazida/sangue , Hidroflumetiazida/urina , Masculino , Métodos , Pessoa de Meia-Idade , Espectrometria de FluorescênciaRESUMO
A rapid, accurate, sensitive, and reproducible assay for hydroflumethiazide in plasma and urine was developed after studies of its UV and fluorescence spectral properties and partitioning behavior. The assay is based on initial extraction from acidified plasma or urine into ether, back-extraction into basic solution followed by acidification to about pH 1, and measurement of the fluorescence derived from the unionized molecule. Analysis of variance indicated no significant differences in assays performed on the same day. The mean recovery was 98.8 +/- 7.4% for plasma over a concentration range of 0.2-2.0 mug/ml. The method is convenient for routine clinical use and has sufficient sensitivity to quantify hydroflumethiazide levels after administration of therapeutic doses.
