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1.
Am J Physiol Regul Integr Comp Physiol ; 297(1): R60-9, 2009 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-19403864

RESUMO

Severe global fetal asphyxia, if caused by a brief occlusion of the umbilical cord, results in prolonged cerebral hypoperfusion in fetal sheep. In this study, we sought evidence to support the hypothesis that cerebral hypoperfusion is a consequence of suppressed cerebral metabolism. In the 24 h following complete occlusion of the umbilical cord for 10 min, sagittal sinus blood flow velocity was significantly decreased for up to 12 h. Capillary blood flow, measured using microspheres, decreased at 1 and 5 h after cord occlusion in many brain regions, including cortical gray and white matter. Microdialysis probes implanted in the cerebral cortex revealed an increase in extracellular glucose concentrations in gray matter for 7-8 h postasphyxia, while lactate increased only briefly, suggesting decreased cerebral glucose utilization over this time. Although these data, as well as the concurrent suppression of breathing movements and electrocortical activity, support the concept of hypometabolic hypoperfusion, the significant increase of pyruvate and glycerol concentrations in dialysate fluid obtained from the cerebral cortex at 3-8 h after cord occlusion suggests an eventual loss of membrane integrity. The prolonged increase of breathing movements for many hours suggests loss of the pontine/thalamic control that produces the distinct pattern of fetal breathing movements.


Assuntos
Asfixia/fisiopatologia , Encéfalo/irrigação sanguínea , Encéfalo/metabolismo , Circulação Cerebrovascular , Metabolismo Energético , Hipóxia Fetal/fisiopatologia , Mecânica Respiratória , Cordão Umbilical/irrigação sanguínea , Animais , Asfixia/sangue , Asfixia/líquido cefalorraquidiano , Asfixia/embriologia , Biomarcadores/sangue , Biomarcadores/líquido cefalorraquidiano , Velocidade do Fluxo Sanguíneo , Pressão Sanguínea , Encéfalo/embriologia , Dióxido de Carbono/sangue , Modelos Animais de Doenças , Eletroencefalografia , Feminino , Sangue Fetal/metabolismo , Hipóxia Fetal/sangue , Hipóxia Fetal/líquido cefalorraquidiano , Idade Gestacional , Glucose/líquido cefalorraquidiano , Glicerol/líquido cefalorraquidiano , Frequência Cardíaca , Concentração de Íons de Hidrogênio , Ácido Láctico/líquido cefalorraquidiano , Microdiálise , Oxigênio/sangue , Gravidez , Ácido Pirúvico/líquido cefalorraquidiano , Ovinos , Fatores de Tempo , Cordão Umbilical/cirurgia
2.
Pediatr Res ; 45(6): 820-6, 1999 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-10367772

RESUMO

Concentrations of the endogenous glutamate receptor antagonist kynurenic acid (KA) were measured in various brain regions and in cisternal cerebrospinal fluid of fetal, newborn, and adult sheep. KA concentrations were significantly higher in the fetal brain and cerebrospinal fluid at 90 and 140 d gestation compared with postnatal ages. In fetuses of 132-139 d gestation, KA concentrations in cerebrospinal fluid collected by drainage from an indwelling cisternal catheter increased significantly after infusion of the organic acid transport inhibitor probenecid (100 or 200 mg/kg, i.v.) indicating active transport of KA out of the fetal brain. In fetuses in which the umbilical circulation had been chronically restricted from 120 to 140 d gestation by partial embolization of the placenta, plasma concentrations of the KA precursor kynurenine were significantly lower than in control fetuses, and KA concentrations in the hypothalamus and hippocampus were significantly reduced; other brain regions were not affected. These results indicate that the production of KA is higher in the fetal brain compared with the newborn and adult brain. Because KA diminishes the risk of excitotoxic neuronal damage under hypoxic-ischemic conditions, the high levels of KA in the brain before birth may have a neuroprotective function. The decrease of KA concentrations in the hypothalamus and hippocampus after umbilical embolization suggests that, after chronic hypoxia in utero, these regions of the brain may become more vulnerable to subsequent episodes of acute hypoxia or ischemia encountered in late gestation or during parturition.


Assuntos
Encéfalo/metabolismo , Feto/metabolismo , Ácido Cinurênico/líquido cefalorraquidiano , Ácido Cinurênico/metabolismo , Animais , Animais Recém-Nascidos , Encéfalo/efeitos dos fármacos , Encéfalo/embriologia , Lesões Encefálicas/embriologia , Lesões Encefálicas/etiologia , Lesões Encefálicas/metabolismo , Embolia/líquido cefalorraquidiano , Embolia/metabolismo , Feminino , Hipóxia Fetal/líquido cefalorraquidiano , Hipóxia Fetal/metabolismo , Circulação Placentária , Gravidez , Probenecid/farmacologia , Ovinos
3.
Pediatr Res ; 36(5): 595-600, 1994 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-7877877

RESUMO

During severe oxygen shortage, the fetal brain resorts to anaerobic metabolism and ATP becomes catabolized. High levels of nucleosides, hypoxanthine, and xanthine (ATP catabolites) in cerebrospinal fluid (CSF) may therefore be associated with increased neonatal neurologic morbidity. In 22 fetal lambs (3 to 5 d after surgery, gestational age 123.5 +/- 3.5 d), arterial oxygen content was progressively reduced to 35% of the baseline value with a balloon occluder around the maternal common internal iliac artery. This resulted in a 1-h period of asphyxia, leading to a pH of 7.02 +/- 0.03 and a base excess of -17.0 +/- 1.0 mM. Mortality was 50%. CSF was sampled from the spinal cistern and analyzed using HPLC. During reoxygenation, hypoxanthine and xanthine may serve as substrate for xanthine oxidase with concomitant production of oxygen-derived free radicals, which may aggravate cerebral damage. The main difference between surviving and nonsurviving animals was the speed of increment of ATP catabolites in CSF: in the surviving group levels increased steadily, recovery values being significantly elevated compared with asphyxia values, whereas in the nonsurviving group the rise was rapid and levels during asphyxia did not differ significantly from levels during recovery. We conclude that 1) catheterization of the spinal cistern leads to increased levels of CSF hypoxanthine, xanthine, and inosine, and 2) during fetal asphyxia, levels of these ATP catabolites and lactate in CSF increase. 3) Maximum levels are reached during the recovery period and are similar for surviving and nonsurviving animals, but during asphyxia CSF levels of hypoxanthine and lactate were higher in the nonsurviving fetuses.(ABSTRACT TRUNCATED AT 250 WORDS)


Assuntos
Asfixia/líquido cefalorraquidiano , Hipóxia Fetal/líquido cefalorraquidiano , Feto/cirurgia , Lactatos/líquido cefalorraquidiano , Nucleosídeos/líquido cefalorraquidiano , Nucleotídeos de Purina/líquido cefalorraquidiano , Trifosfato de Adenosina/líquido cefalorraquidiano , Animais , Feminino , Ácido Láctico , Gravidez , Ovinos , Sobreviventes
4.
Endocrinology ; 116(1): 65-72, 1985 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-3964753

RESUMO

The concentrations of vasopressin in the plasma and cerebrospinal fluid (CSF) of the chronically catheterized fetal lamb were measured under basal and hypoxic conditions. Under basal conditions, samples were obtained from 13 fetal lambs of 117-146 days gestation. The mean +/- SEM vasopressin level in CSF was 19.5 +/- 1.5 pg/ml; the mean plasma vasopressin level of 1.9 +/- 0.2 pg/ml was significantly less (P less than 0.001). No consistent change in concentrations of vasopressin in CSF was observed with gestational maturation in 3 animals sampled sequentially or in individual samples obtained over the last 32 days of gestation. The mean vasopressin concentration in the CSF of the pregnant ewe was 5.1 +/- 0.4 pg/ml. The gradients for osmolality, sodium, and potassium between fetal plasma and CSF were: osmolality, 298.4 +/- 1.6 to 304.3 +/- 1.4 mosmol/kg; sodium, 140.9 +/- 0.5-142.5 +/- 0.5 meq/liter; and potassium, 4.3 +/- 0.1 to 3.3 +/- 0.1 meq/liter. Fetal hypoxia was induced by exposure of the ewe to 10% O2 in N2 for 30 min. The concentration of vasopressin increased from 1.7 +/- 0.3 to 277 +/- 144 pg/ml (P less than 0.001) in fetal plasma and from 21.4 +/- 3.8 to 47.1 +/- 9.9 pg/ml (P less than 0.04) in fetal CSF. When the ewe was exposed to room air under comparable experimental conditions, no similar changes in plasma or CSF vasopressin levels were observed in the fetus. Infusion of vasopressin into the fetal jugular vein at 1.0 mU/min for 30 min increased plasma concentrations from 2.3 +/- 0.5 to 83 +/- 17 pg/ml, while the CSF vasopressin values were 31.9 +/- 5.9 (basally) and 30.7 +/- 4.8 pg/ml (after infusion). Mean plasma and CSF osmolality, sodium, and potassium were not changed by any of these experimental interventions. We conclude that 1) under basal conditions, high concentrations of vasopressin are present in the CSF of the fetal lamb, the blood-CSF barrier appears to be impermeable to vasopressin, and concentrations of the hormone in fetal plasma are less than those in CSF; and 2) hypoxia is a potent stimulus of vasopressin release in both fetal plasma and CSF. The route of vasopressin released into the fetal CSF may be distinct from that released into plasma.


Assuntos
Hipóxia Fetal/líquido cefalorraquidiano , Vasopressinas/líquido cefalorraquidiano , Animais , Pressão Sanguínea , Barreira Hematoencefálica , Feminino , Hipóxia Fetal/sangue , Hipóxia Fetal/fisiopatologia , Idade Gestacional , Frequência Cardíaca , Gravidez , Valores de Referência , Ovinos , Vasopressinas/sangue
5.
J Clin Pathol ; 36(1): 1-8, 1983 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-6681617

RESUMO

CSF obtained for clinical purposes from newborn, children and adults has been analysed by high pressure liquid chromatography for hypoxanthine, xanthine, inosine, uridine and urate. Large rises in hypoxanthine and to a lesser extent xanthine occur for about 24 h after hypoxia. High concentrations were associated with later evidence of brain damage or subsequent death. Changes in CSF could be independent of those in plasma. Small or negligible rises were associated with localised and generalised infections including bacterial meningitis, fits, or both. Marked and rapid rises were found after death. These estimations may "predict" the extent of brain damage or brain death.


Assuntos
Hipóxia Fetal/líquido cefalorraquidiano , Hipoxantinas/líquido cefalorraquidiano , Adulto , Asfixia Neonatal/líquido cefalorraquidiano , Dano Encefálico Crônico/líquido cefalorraquidiano , Dano Encefálico Crônico/etiologia , Feminino , Hipóxia Fetal/complicações , Humanos , Recém-Nascido , Inosina/líquido cefalorraquidiano , Mudanças Depois da Morte , Gravidez , Prognóstico , Ácido Úrico/líquido cefalorraquidiano , Uridina/líquido cefalorraquidiano , Xantinas/líquido cefalorraquidiano
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