RESUMO
Five kinds of oligopeptides H-Asp-Ser-Asp-OH (1), H-Asp-Gly-Lys-OH (2), H-Ser-Asp-Gly-Lys-OH (3), H-Asp-Ser-Asp-Gly-Lys-OH (4), and H-Ala-Asp-Ser-Asp-Gly-Lys-OH (5) related to immunoglobulin E (IgE) were synthesized by the conventional solution method with the objective of obtaining a new type of antiallergic agent. Their pharmacological activity was examined by measuring the inhibition of the production of IgE and relaxation of the smooth muscle contraction of rabbit aorta. H-Ala-Asp-Ser-Asp-Gly-Lys-OH (5) displayed potent inhibition against the production of IgE antibody (69.6%) and relaxation against the contraction of rabbit aorta.
Assuntos
Imunoglobulina E/síntese química , Peptídeos/síntese química , Sequência de Aminoácidos , Animais , Formação de Anticorpos/efeitos dos fármacos , Aorta/efeitos dos fármacos , Aorta/fisiologia , Imunoglobulina E/farmacologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Contração Muscular/efeitos dos fármacos , Músculo Liso Vascular/efeitos dos fármacos , Músculo Liso Vascular/fisiologia , Peptídeos/farmacologia , CoelhosRESUMO
Conventional methods of peptide chemistry have been used to synthesize the C-terminal nonapeptide from human immunoglobulin E, which is a potential cytophilic binding site of the IgE molecule responsible for its primary recognition and binding to specific target cell receptors.
