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1.
PLoS One ; 17(1): e0262984, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35077517

RESUMO

Carbapenems are the last resort antimicrobials for the treatment of extended spectrum ß-lactamases (ESBLs) producing Enterobacteriaceae. Emergence of carbapenems resistant group B2 uropathogenic E. coli (UPEC) is a major concern because of their high virulence. Prevalence of these enzymes and multidrug resistance (MDR) among B2 UPEC isolates from Iraqi outpatients with acute urinary tract infection (UTI) was evaluated in this research. Urine cultures were performed and the isolates were identified biochemically. Escherichia coli isolates were tested for phylogroup reference by quadraplex PCR, then B2 isolates were detected for antimicrobial resistance by disc diffusion test and carbapenemase genes by PCR. Escherichia coli was the most prevalent among Gram-negative isolates (66.6%) and B2 was the most detected phylogroup among E. coli isolates (33.9%). Most of B2 isolates showed high resistance rates to tested antimicrobials, especially ß-lactams with MDR revealed in 100% of them. Whereas, low resistance rates were noted against carbapenems, aminoglycosides and nitrofurantoin. Carbapenemase genes were detected in 76.3% of B2 isolates. Of which, blaOXA-48 was the most frequent (57.8%), followed by blaPER (47.3%), blaKPC (15.7%), blaVEB and blaVIM (10.5%, for each). Whereas, blaGES and blaIMP genes were not found. Coproduction of these genes occurred among 17 isolates. The combination of blaOXA-48 and blaPER was the most frequent (41.1%). All carbapenemase producing isolates were MDR. These results revealed high prevalence of carbapenemase genes and MDR among B2 UPEC recovered in this study. In the study area. it is strongly advised to use aminoglycosides and nitrofurantoin for empirical treatment of UPEC.


Assuntos
Proteínas de Bactérias/genética , Farmacorresistência Bacteriana Múltipla , Infecções por Escherichia coli/genética , Pacientes Ambulatoriais , Infecções Urinárias/genética , Escherichia coli Uropatogênica , Fatores de Virulência/genética , beta-Lactamases/genética , Infecções por Escherichia coli/enzimologia , Infecções por Escherichia coli/epidemiologia , Feminino , Humanos , Iraque/epidemiologia , Masculino , Prevalência , Infecções Urinárias/enzimologia , Infecções Urinárias/epidemiologia , Escherichia coli Uropatogênica/enzimologia , Escherichia coli Uropatogênica/genética , Escherichia coli Uropatogênica/isolamento & purificação
2.
Afr Health Sci ; 21(2): 497-504, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-34795701

RESUMO

BACKGROUND: T2DM patients are more likely to have UTIs caused by resistant organisms such as ESBLs producing bacteria. Challenging reliable identification and prompt characterization of in-vitro susceptibilities of these bacteria are the first steps of deciding the appropriate antimicrobial therapy for UTIs caused by them. OBJECTIVES: To isolate and identify E. coli and K. pneumoniae from urine of T2DM patients with UTIs, to determine antibiotic resistance pattern among isolates, and to identify ESBLs production phenotypically and genotypically. MATERIAL AND METHOD: All samples were cultured on Cystine-Lactose-Electrolyte-Deficient Agar medium (CLED) by using calibrated loop. Growth of 100 colonies or more, i.e. 105 colony forming units (CFU)/mL urine was considered as significant bacteriuria. Isolation and identification were done according to standard method. All isolates were tested for antibiotic susceptibility testing by the disc diffusion method according to CLSI guidelines. Phenotypic detection of ESBLs was done by double-disk synergy test. Genotypic detection of blaTEM, blaSHV and blaCTX-M genes by using PCR. RESULTS: Results of this study showed that E. coli and K. pneumoniae were the dominant bacterial isolates, they constituted 103 (91.2%) out of 113 urine isolates. E. coli (58. 4%) K. pneumoniae (32.7%), Enterococcus spp. (4.4%), Proteus spp. (2.7%) and Pseudomonas spp. (1.8%). About 25 (24.3%) out of 103 E. coli and K. pneumoniae isolates were ESBLs positive by DDST, and 22 (88.0%) out of them had ESBLs encoding genes by conventional PCR. The most common gene detected was blaTEM (59.1%), followed by blaSHV (27.3%). CTX-M had not been detected in any of testes isolates. CONCLUSION: blaTEM and blaSHV genes were detected in 22 out of 25 ESBLs producing E. coli and K. pneumoniae isolates phenotypically detected by DDST. blaTEM was found to be the predominant gene (59.1%), while blaCTX-Mene was not detected in any of tested isolates.


Assuntos
Diabetes Mellitus Tipo 2/complicações , Escherichia coli/genética , Klebsiella pneumoniae/genética , Infecções Urinárias/diagnóstico , Infecções Urinárias/epidemiologia , beta-Lactamases/efeitos dos fármacos , DNA Bacteriano/análise , Farmacorresistência Bacteriana Múltipla , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/diagnóstico , Infecções por Escherichia coli/tratamento farmacológico , Humanos , Infecções por Klebsiella/diagnóstico , Infecções por Klebsiella/tratamento farmacológico , Klebsiella pneumoniae/isolamento & purificação , Reação em Cadeia da Polimerase , Infecções Urinárias/tratamento farmacológico , Infecções Urinárias/enzimologia , beta-Lactamases/genética
3.
Am J Physiol Renal Physiol ; 318(6): F1441-F1453, 2020 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-32390512

RESUMO

Carbonic anhydrase II knockout (Car2-/-) mice have depleted numbers of renal intercalated cells, which are increasingly recognized to be innate immune effectors. We compared pyelonephritis susceptibility following reciprocal renal transplantations between Car2-/- and wild-type mice. We examined the effect of pharmacological CA suppression using acetazolamide in an experimental murine model of urinary tract infection. Car2-/- versus wild-type mice were compared for differences in renal innate immunity. In our transplant scheme, mice lacking CA-II in the kidney had increased pyelonephritis risk. Mice treated with acetazolamide had lower kidney bacterial burdens at 6 h postinfection, which appeared to be due to tubular flow from diuresis because comparable results were obtained when furosemide was substituted for acetazolamide. Isolated Car2-/- kidney cells enriched for intercalated cells demonstrated altered intercalated cell innate immune gene expression, notably increased calgizzarin and insulin receptor expression. Intercalated cell number and function along with renal tubular flow are determinants of pyelonephritis risk.


Assuntos
Acetazolamida/farmacologia , Anidrase Carbônica II/deficiência , Inibidores da Anidrase Carbônica/farmacologia , Infecções por Escherichia coli/prevenção & controle , Rim/efeitos dos fármacos , Pielonefrite/prevenção & controle , Infecções Urinárias/prevenção & controle , Acidose/enzimologia , Acidose/genética , Animais , Anidrase Carbônica II/antagonistas & inibidores , Anidrase Carbônica II/genética , Modelos Animais de Doenças , Infecções por Escherichia coli/enzimologia , Infecções por Escherichia coli/genética , Infecções por Escherichia coli/microbiologia , Regulação da Expressão Gênica no Desenvolvimento , Predisposição Genética para Doença , Imunidade Inata , Rim/enzimologia , Rim/imunologia , Rim/microbiologia , Transplante de Rim , Camundongos Endogâmicos C57BL , Camundongos Knockout , Pielonefrite/enzimologia , Pielonefrite/genética , Pielonefrite/microbiologia , Receptor de Insulina/genética , Receptor de Insulina/metabolismo , Proteínas S100/genética , Proteínas S100/metabolismo , Infecções Urinárias/enzimologia , Infecções Urinárias/genética , Infecções Urinárias/microbiologia , Escherichia coli Uropatogênica/patogenicidade
4.
Front Immunol ; 10: 2786, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31849967

RESUMO

The lower urinary tract is routinely exposed to microbes residing in the gastrointestinal tract, yet the urothelium resists invasive infections by gut microorganisms. This infection resistance is attributed to innate defenses in the bladder urothelium, kidney epithelium, and resident or circulating immune cells. In recent years, surmounting evidence suggests that these cell types produce and secrete soluble host defense peptides, including members of the Ribonuclease (RNase) A Superfamily, to combat invasive bacterial challenge. While some of these peptides, including RNase 4 and RNase 7, are abundantly produced by epithelial cells, the expression of others, like RNase 3 and RNase 6, increase at infection sites with immune cell recruitment. The objective of this mini-review is to highlight recent evidence showing the biological importance and responses of RNase A Superfamily members to infection in the kidney and bladder.


Assuntos
Família Multigênica , Ribonuclease Pancreático/genética , Ribonuclease Pancreático/metabolismo , Infecções Urinárias/enzimologia , Infecções Urinárias/etiologia , Animais , Peptídeos Catiônicos Antimicrobianos , Humanos , Imunidade Inata , Leucócitos/enzimologia , Mucosa/metabolismo
5.
J Am Soc Nephrol ; 30(8): 1385-1397, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31239387

RESUMO

BACKGROUND: Evidence suggests that antimicrobial peptides, components of the innate immune response, protect the kidneys and bladder from bacterial challenge. We previously identified ribonuclease 7 (RNase 7) as a human antimicrobial peptide that has bactericidal activity against uropathogenic Escherichia coli (UPEC). Functional studies assessing RNase 7's contributions to urinary tract defense are limited. METHODS: To investigate RNase 7's role in preventing urinary tract infection (UTI), we quantified urinary RNase 7 concentrations in 29 girls and adolescents with a UTI history and 29 healthy female human controls. To assess RNase 7's antimicrobial activity in vitro in human urothelial cells, we used siRNA to silence urothelial RNase 7 production and retroviral constructs to stably overexpress RNase 7; we then evaluated UPEC's ability to bind and invade these cells. For RNase 7 in vivo studies, we developed humanized RNase 7 transgenic mice, subjected them to experimental UTI, and enumerated UPEC burden in the urine, bladder, and kidneys. RESULTS: Compared with controls, study participants with a UTI history had 1.5-fold lower urinary RNase 7 concentrations. When RNase 7 was silenced in vitro, the percentage of UPEC binding or invading human urothelial cells increased; when cells overexpressed RNase 7, UPEC attachment and invasion decreased. In the transgenic mice, we detected RNase 7 expression in the kidney's intercalated cells and bladder urothelium. RNase 7 humanized mice exhibited marked protection from UPEC. CONCLUSIONS: These findings provide evidence that RNase 7 has a role in kidney and bladder host defense against UPEC and establish a foundation for investigating RNase 7 as a UTI prognostic marker or nonantibiotic-based therapy.


Assuntos
Infecções por Escherichia coli/enzimologia , Rim/enzimologia , Ribonucleases/genética , Bexiga Urinária/enzimologia , Infecções Urinárias/enzimologia , Infecções Urinárias/microbiologia , Escherichia coli Uropatogênica , Adolescente , Animais , Antibacterianos/farmacologia , Peptídeos Catiônicos Antimicrobianos/genética , Criança , Pré-Escolar , Feminino , Inativação Gênica , Humanos , Imunidade Inata , Lactente , Rim/microbiologia , Masculino , Camundongos , Camundongos Transgênicos , Fenótipo , Prognóstico , Bexiga Urinária/microbiologia , Urotélio/metabolismo , Urotélio/patologia , Adulto Jovem
6.
Am J Physiol Regul Integr Comp Physiol ; 314(3): R353-R365, 2018 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-29118019

RESUMO

Chronic pelvic pain causes significant patient morbidity and is a challenge to clinicians. Using a murine neurogenic cystitis model that recapitulates key aspects of interstitial cystitis/bladder pain syndrome (IC), we recently showed that pseudorabies virus (PRV) induces severe pelvic allodynia in BALB/c mice relative to C57BL/6 mice. Here, we report that a quantitative trait locus (QTL) analysis of PRV-induced allodynia in F2CxB progeny identified a polymorphism on chromosome 13, rs6314295 , significantly associated with allodynia (logarithm of odds = 3.11). The nearby gene encoding acyloxyacyl hydrolase ( Aoah) was induced in the sacral spinal cord of PRV-infected mice. AOAH-deficient mice exhibited increased vesicomotor reflex in response to bladder distension, consistent with spontaneous bladder hypersensitivity, and increased pelvic allodynia in neurogenic cystitis and postbacterial chronic pain models. AOAH deficiency resulted in greater bladder pathology and tumor necrosis factor production in PRV neurogenic cystitis, markers of increased bladder mast cell activation. AOAH immunoreactivity was detectable along the bladder-brain axis, including in brain sites previously correlated with human chronic pelvic pain. Finally, AOAH-deficient mice had significantly higher levels of bladder vascular endothelial growth factor, an emerging marker of chronic pelvic pain in humans. These findings indicate that AOAH modulates pelvic pain severity, suggesting that allelic variation in Aoah influences pelvic pain in IC.


Assuntos
Hidrolases de Éster Carboxílico/metabolismo , Cistite Intersticial/enzimologia , Infecções por Escherichia coli/enzimologia , Hiperalgesia/enzimologia , Dor Pélvica/enzimologia , Pseudorraiva/enzimologia , Bexiga Urinária/inervação , Infecções Urinárias/enzimologia , Animais , Comportamento Animal , Hidrolases de Éster Carboxílico/deficiência , Hidrolases de Éster Carboxílico/genética , Cistite Intersticial/genética , Cistite Intersticial/fisiopatologia , Cistite Intersticial/psicologia , Modelos Animais de Doenças , Infecções por Escherichia coli/genética , Infecções por Escherichia coli/fisiopatologia , Infecções por Escherichia coli/psicologia , Feminino , Predisposição Genética para Doença , Hiperalgesia/genética , Hiperalgesia/fisiopatologia , Hiperalgesia/psicologia , Masculino , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos Knockout , Percepção da Dor , Limiar da Dor , Dor Pélvica/genética , Dor Pélvica/fisiopatologia , Fenótipo , Pseudorraiva/genética , Pseudorraiva/fisiopatologia , Pseudorraiva/psicologia , Locos de Características Quantitativas , Índice de Gravidade de Doença , Fator de Necrose Tumoral alfa/metabolismo , Bexiga Urinária/metabolismo , Infecções Urinárias/genética , Infecções Urinárias/fisiopatologia , Infecções Urinárias/psicologia , Fator A de Crescimento do Endotélio Vascular/metabolismo
7.
Rev Esp Quimioter ; 30(6): 450-457, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29115369

RESUMO

OBJECTIVE: The aim of this study is to know epidemiologic and clinical differences among those patients colonized or infected by carbapenemase-producing Enterobacteriaceae (CPE) and develop a predictive model to facilitate the clinical approach concerning to start antimicrobial therapy. METHODS: Observational retrospective cohort study was performed involving all patients with Urine carbapenemase-producing Enterobacteriaceae isolation (UCPEI) between November 2013 and July 2015. Patients were classifieds as colonized or infected considering Center for Disease Control and Prevention (CDC) definition for urinary tract infection (UTI). RESULTS: A total of 72 patients were included, mean age 76.4 (IQR 23-99) years and 40 (55.6%) were women. Thirty-four (47.2%) were colonized and 38 (52.8%) met the criteria of UTI and were considered infected. The independent variables associated to infection were female sex, peripheral vascular disease, admission in medical ward, permanent urinary catheter carrier, previous antimicrobial therapy, and length of stay. Isolation of OXA-48 carbapenemase-producing Enterobacteriaceae behaved as a non UTI (colonization) factor in comparison with KPC or VIM CPE. The developed predictive model showed an area under the curve (AUC) of 0.901 (95% CI: 0.832-0.970; p < 0.001). CONCLUSIONS: The predictive model that includes all this factors has demonstrated a good accuracy for infection diagnosis in these patients, an important issue considering that establishing the diagnosis of infection is not always easy in the profile of patients in which a CPE is isolated.


Assuntos
Proteínas de Bactérias/metabolismo , Enterobacteriáceas Resistentes a Carbapenêmicos/enzimologia , Enterobacteriáceas Resistentes a Carbapenêmicos/genética , Infecções por Enterobacteriaceae/enzimologia , Infecções por Enterobacteriaceae/urina , Infecções Urinárias/enzimologia , Infecções Urinárias/urina , beta-Lactamases/metabolismo , Adulto , Idoso , Idoso de 80 Anos ou mais , Antibacterianos/uso terapêutico , Estudos de Coortes , Contagem de Colônia Microbiana , Farmacorresistência Bacteriana , Infecções por Enterobacteriaceae/diagnóstico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores Sexuais , Infecções Urinárias/diagnóstico , Doenças Vasculares/complicações , Doenças Vasculares/epidemiologia , Adulto Jovem
8.
Clin Chim Acta ; 471: 286-291, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28622967

RESUMO

Urinary tract infection (UTI) is the most common bacterial infection independent of age. It is also one of the most common causes of hospitalizations for infections among elderly people and the most common indication for antibiotic prescriptions in primary care. Both diagnostics and management of lower and upper urinary tract infections provide challenges in clinical practice due to their high prevalence and recurrence, and worldwide increase of antibiotic resistance. The clinical symptoms of UTI are often uncharacteristic or asymptomatic. The accurate diagnosis and early treatment are crucial due to risk of septicaemia and long-term consequences. Currently the diagnosis of urinary tract infection is based on the presence of clinical symptoms in combination with the results of nitrite strip test indicating the presence of bacteria in urine and semi-quantitative measurement of white blood cells count in urine. Although urine culture is the gold standard in UTI diagnostics it is both time-consuming and costly. Searching for novel biomarkers of UTI has attracted much attention in recent years. The article reviews several promising serum and urine biomarkers of UTI such as leukocyte esterase, C-reactive protein, procalcitonin, interleukins, elastase alpha (1)-proteinase inhibitor, lactofferin, secretory immunoglobulin A, heparin-binding protein, xanthine oxidase, myeloperoxidase, soluble triggering receptor expressed on myeloid cells-1, α-1 microglobulin (α1Mg) and tetrazolium nitroblue test (TNB).


Assuntos
Infecções Urinárias/diagnóstico , Infecções Urinárias/urina , Animais , Biomarcadores/metabolismo , Biomarcadores/urina , Humanos , Infecções Urinárias/enzimologia
9.
Am J Obstet Gynecol ; 213(5): 650-2, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25771212

RESUMO

Extended-spectrum ß-lactamases (ESBLs) are rapidly evolving plasmid transferrable enzymes that confer unique patterns of antibiotic resistance on various bacterial species. Such organisms pose special challenges to laboratory identification, as well as antibiotic selection, administration, and follow-up. Although such infections are increasingly common in the obstetric population, issues surrounding ESBLs are not widely recognized by practicing obstetricians, and controversies exist regarding diagnosis and management. This article provides the practitioner with a summary of clinically pertinent information that will assist in the proper care of pregnant patients with ESBL infection.


Assuntos
Farmacorresistência Bacteriana , Infecções por Enterobacteriaceae/enzimologia , Infecções por Escherichia coli/enzimologia , Complicações Infecciosas na Gravidez/enzimologia , beta-Lactamases/metabolismo , Adulto , Cefalosporinas/uso terapêutico , Feminino , Humanos , Testes de Sensibilidade Microbiana , Gravidez , Complicações Infecciosas na Gravidez/tratamento farmacológico , Infecções Urinárias/tratamento farmacológico , Infecções Urinárias/enzimologia
10.
Libyan J Med ; 10(1): 26412, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25651907

RESUMO

INTRODUCTION: Multidrug resistance (MDR) and emergence of extended-spectrum ß-lactamases (ESBLs) that mediate resistance to ß-lactam drugs among Escherichia coli and other uropathogens have been reported worldwide. However, there is little information on the detection of ESBLs genes in E. coli from patients with urinary tract infections (UTIs) in the Arab countries using polymerase chain reaction (PCR), and in Libya such information is lacking. METHODS: All patients attending Zawiya Teaching Hospital in Zawiya city between November 2012 and June 2013 suspected of having UTIs and from whom midstream urine samples were taken as part of the clinical workup were included in this prospective study. Samples were examined for uropathogens by standard bacteriological procedures. VITEK-2 automated microbiology system was used to identify the isolated uropathogens and determine the susceptibility of E. coli and Klebsiella spp. isolates to antimicrobials. In addition, phenotypically ESBLs-positive E. coli isolates were tested for ESBLs genes by PCR. RESULTS: The present study enrolled 1,790 patients with UTIs. Uropathogens were found in 371 (20.7%) urine specimens examined. Mixed pathogens were detected in two specimens with 373 total pathogens isolated. E. coli and Klebsiella spp. were the predominant uropathogens at 55.8% (208/373) and 18.5% (69/373), respectively. Other pathogens were detected in 25.7% (96/373) of urine samples. Of the E. coli and Klebsiella spp. tested, 69.2 and 100% were resistant to ampicillin, 6.7 and 33.3% to ceftriaxone, and 23.1 and 17.4% to ciprofloxacin, respectively. MDR (resistance to ≥3 antimicrobial groups) was found in 69 (33.2%) of E. coli and in 29 (42%) of Klebsiella spp. isolates. ESBLs were detected phenotypically in 14 (6.7%) of E. coli and in 15 (21.7%) of Klebsiella spp. isolates. Thirteen out of the 14 phenotypically ESBL-positive E. coli were positive for ESBL genes by PCR. bla TEM gene was detected in seven isolates, bla OXA gene in 10 isolates and bla CTX-M gene in six isolates. bla SHV gene was not detected in the present study. CONCLUSION: The isolation of MDR ESBL-producing uropathogens undoubtedly will limit the choices clinicians have to treat their patients with UTIs. Therefore, there is an urgent need for surveillance studies on antimicrobial resistance and prevalence of ESBLs among uropathogens to guide the clinical treatment of UTIs in Libya in the future.


Assuntos
Infecções por Escherichia coli/epidemiologia , Escherichia coli/enzimologia , Infecções por Klebsiella/epidemiologia , Klebsiella pneumoniae/enzimologia , Infecções Urinárias/epidemiologia , beta-Lactamases/efeitos dos fármacos , beta-Lactamases/genética , Farmacorresistência Bacteriana Múltipla , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/tratamento farmacológico , Infecções por Escherichia coli/enzimologia , Feminino , Humanos , Infecções por Klebsiella/tratamento farmacológico , Infecções por Klebsiella/enzimologia , Klebsiella pneumoniae/isolamento & purificação , Líbia/epidemiologia , Masculino , Testes de Sensibilidade Microbiana , Reação em Cadeia da Polimerase , Guias de Prática Clínica como Assunto , Prevalência , Estudos Prospectivos , Vigilância de Evento Sentinela , Infecções Urinárias/tratamento farmacológico , Infecções Urinárias/enzimologia , Resistência beta-Lactâmica
11.
Pharm Biol ; 53(3): 326-33, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25366133

RESUMO

CONTEXT: Bacterial ureases play an important role in pathogenesis of urinary infections. Selection of plants was done on the basis of their uses by the local people for the treatment of various bacterial and urinary infections. OBJECTIVE: Our investigation screens and evaluates 15 Indian medicinal plants for their possible urease inhibitory activity as well as their ability to inhibit bacteria causing urinary infections. MATERIALS AND METHODS: Plant extracts in three different solvents (methanol, aqueous, and cow urine) were screened for their effect on Jack-bean urease using the phenol-hypochlorite method. Subsequently, seven bacterial strains were screened for their ability to release urease and further antimicrobial-linked urease inhibition activity and minimum inhibitory concentration of the tested extracts were evaluated by the agar well diffusion and microdilution method, respectively. RESULTS: Five plants out of 15 crude extracts revealed good urease inhibitory activity (≥ 20% at 1 mg/ml conc.) and IC50 values for these extracts ranged from 2.77 to 0.70 mg/ml. Further testing of these extracts on urease-producing bacterial strains (Staphylococcus aureus NCDC 109, S. aureus MTCC 3160, Proteus vulgaris MTCC 426, Klebsiella pneumoniae MTCC 4030, and Pseudomonas aeruginosa MTCC 7453) showed good anti-urease potency with an MIC ranging from 500 to 7.3 µg/ml. DISCUSSION AND CONCLUSION: The results of screening as well as susceptibility assay clearly revealed a strong urease inhibitory effect of Acacia nilotica L. (Fabaceae), Emblica officinalis Gaertn. (Phyllanthaceae), Psidium guajava L. (Myrtaceae), Rosa indica L. (Rosaceae), and Terminalia chebula Retz. (Combretaceae). Our findings may help to explain the beneficial effect of these plants against infections associated with the urease enzyme.


Assuntos
Antibacterianos/farmacologia , Canavalia/enzimologia , Plantas Medicinais , Urease/antagonistas & inibidores , Infecções Urinárias/enzimologia , Animais , Antibacterianos/isolamento & purificação , Antibacterianos/uso terapêutico , Proteínas de Bactérias/antagonistas & inibidores , Proteínas de Bactérias/metabolismo , Canavalia/efeitos dos fármacos , Bovinos , Avaliação Pré-Clínica de Medicamentos/métodos , Índia , Pseudomonas aeruginosa/crescimento & desenvolvimento , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/crescimento & desenvolvimento , Urease/metabolismo , Infecções Urinárias/tratamento farmacológico , Infecções Urinárias/microbiologia
12.
Urol Nurs ; 34(3): 135-8, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25112022

RESUMO

Purple urine bag syndrome (PUBS) is a constellation of findings resulting in purple discoloration of the urine and/or urine drainage bag(s) occurring in patients with long-term urinary indwelling catheters. Other causative factors may include constipation, female gender, the presence of bacteria containing sulphatase and phosphatase enzymes, and alkaline urine. While the contributing factors for PUBS are linked with high morbidity, PUBS itself is a benign condition. A case study of monolateral PUBS in a patient with bilateral nephrostomy tubes (NTs) is presented.


Assuntos
Nefrostomia Percutânea/efeitos adversos , Infecções Urinárias/etiologia , Urina , Idoso de 80 Anos ou mais , Cor , Humanos , Indicã/metabolismo , Masculino , Nefrostomia Percutânea/instrumentação , Síndrome , Infecções Urinárias/enzimologia
13.
Dis Markers ; 2014: 269362, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24591758

RESUMO

OBJECTIVES: The aim of this study was to evaluate if xanthine oxidase and myeloperoxidase levels quantitation method may alternate routine culture method, which takes more time in the diagnosis of urinary tract infections. MATERIAL AND METHODS: Five hundred and forty-nine outpatients who had admitted to Clinic Microbiology Laboratory were included in the study. The microorganisms were identified by using VITEK System. The urine specimens that were negative from the quantitative urine culture were used as controls. The activities of MPO and XO in spot urine were measured by spectrophotometric method. RESULTS: Through the urine cultures, 167 bacteria were isolated from 163 urine specimens; 386 cultures yielded no bacterial growth. E. coli was the most frequent pathogen. In infection with E. coli both XO and MPO levels were increased the most. The sensitivity, specificity, positive predictive value, and negative predictive value for XO were 100%, 100%, 100%, and 100%, respectively. These values for MPO were 87%, 100%, 100%, and 94%, respectively. CONCLUSION: These data obtained suggest that urine XO and MPO levels may be new markers in the early detection of UTI.


Assuntos
Infecções por Escherichia coli/urina , Peroxidase/urina , Proteinúria/urina , Infecções Urinárias/urina , Xantina Oxidase/urina , Adolescente , Adulto , Idoso , Biomarcadores/urina , Criança , Pré-Escolar , Diagnóstico Precoce , Infecções por Escherichia coli/diagnóstico , Infecções por Escherichia coli/enzimologia , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Proteinúria/diagnóstico , Proteinúria/enzimologia , Proteinúria/microbiologia , Sensibilidade e Especificidade , Infecções Urinárias/diagnóstico , Infecções Urinárias/enzimologia , Adulto Jovem
14.
J Intern Med ; 276(2): 120-9, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24635559

RESUMO

This review is largely based on a previous paper published in the journal Spinal Cord. The care of many patients undergoing long-term bladder catheterization is complicated by encrustation and blockage of their Foley catheters. This problem stems from infection by urease-producing bacteria, particularly Proteus mirabilis. These organisms colonize the catheter forming an extensive biofilm; they also generate ammonia from urea, thus elevating the pH of urine. As the pH rises, crystals of calcium and magnesium phosphates precipitate in the urine and in the catheter biofilm. The continued development of this crystalline biofilm blocks the flow of urine through the catheter. Urine then either leaks along the outside of the catheter and the patient becomes incontinent or is retained causing painful distension of the bladder and reflux of urine to the kidneys. The process of crystal deposition can also initiate stone formation. Most patients suffering from recurrent catheter encrustation develop bladder stones. P. mirabilis establishes stable residence in these stones and is extremely difficult to eliminate from the catheterized urinary tract by antibiotic therapy. If blocked catheters are not identified and changed, serious symptomatic episodes of pyelonephritis, septicaemia and endotoxic shock can result. All types of Foley catheters including silver- or nitrofurazone-coated devices are vulnerable to this problem. In this review, the ways in which biofilm formation on Foley catheters is initiated by P. mirabilis will be described. The implications of understanding these mechanisms for the development of an encrustation-resistant catheter will be discussed. Finally, the way forward for the prevention and control of this problem will be considered.


Assuntos
Biofilmes/crescimento & desenvolvimento , Infecções Relacionadas a Cateter/microbiologia , Proteus mirabilis/patogenicidade , Cateterismo Urinário/efeitos adversos , Cateteres Urinários/efeitos adversos , Infecções Urinárias/microbiologia , Compostos de Cálcio/metabolismo , Infecções Relacionadas a Cateter/prevenção & controle , Citratos/urina , Cristalização , Humanos , Compostos de Magnésio/metabolismo , Infecções por Proteus/etiologia , Infecções por Proteus/prevenção & controle , Fatores de Tempo , Cateterismo Urinário/instrumentação , Cateterismo Urinário/métodos , Infecções Urinárias/enzimologia , Infecções Urinárias/prevenção & controle , Urina/química , Urina/microbiologia
15.
Prog Urol ; 23(11): 926-35, 2013 Sep.
Artigo em Francês | MEDLINE | ID: mdl-24010923

RESUMO

GOAL: The aim was to review the literature on nitric oxide and female lower urinary tract. MATERIAL: A literature review through the PubMed library until December, 31 2012 was carried out using the following keywords: lower urinary tract, bladder, urethra, nervous central system, innervation, female, women, nitric oxide, phosphodiesterase, bladder outlet obstruction, urinary incontinence, overactive bladder, urinary tract infection. RESULTS: Two nitric oxide synthase isoforms, the neuronal (nNOS) and the endothelial (eNOS), are constitutively expressed in the lower urinary tract. Nevertheless, nNOS is mainly expressed in the bladder neck and the urethra. In the bladder, NO modulates the afferent neurons activity. In pathological condition, inducible NOS expression induces an increase in detrusor contractility and bladder wall thickness and eNOS facilitates Escherichia coli bladder wall invasion inducing recurrent urinary tract infections. In the urethra, NO play a major role in smooth muscle cells relaxation. CONCLUSION: The NO pathway plays a major role in the female lower urinary tract physiology and physiopathology. While it acts mainly on bladder outlet, in pathological condition, it is involved in bladder dysfunction occurrence.


Assuntos
Óxido Nítrico Sintase Tipo III/metabolismo , Óxido Nítrico Sintase Tipo I/metabolismo , Óxido Nítrico/metabolismo , Transdução de Sinais , Uretra/enzimologia , Bexiga Urinária/enzimologia , Biomarcadores/metabolismo , Feminino , Humanos , Redes e Vias Metabólicas , Óxido Nítrico Sintase/biossíntese , Diester Fosfórico Hidrolases/metabolismo , Uretra/inervação , Uretra/fisiopatologia , Bexiga Urinária/inervação , Bexiga Urinária/fisiopatologia , Obstrução do Colo da Bexiga Urinária/enzimologia , Bexiga Urinária Hiperativa/enzimologia , Incontinência Urinária/enzimologia , Sistema Urinário/enzimologia , Infecções Urinárias/enzimologia , Fenômenos Fisiológicos do Sistema Urinário
16.
J Clin Invest ; 123(6): 2348-9, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23728167

RESUMO

A 5-year-old girl has come to you a week after completing a course of antibiotics for a febrile urinary tract infection (UTI). She now seems well and energetic. A urinalysis is now clear without traces of inflammation, including an absence of protein, blood, leukocyte esterase, and nitrites. Her urine is submitted for a test of cure and comes back positive, with over 100,000 colonies per milliliter of E. coli, the same kind of bacteria that was cultured from her urine when she was symptomatic with the UTI. Perplexed, her mother asks how her child can have bacteria once again in her bladder but not be symptomatic and asks if antibiotics are again necessary.


Assuntos
Bacteriúria/enzimologia , Infecções por Escherichia coli/enzimologia , RNA Polimerase II/metabolismo , Infecções Urinárias/enzimologia , Humanos
17.
J Clin Invest ; 123(6): 2366-79, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23728172

RESUMO

The normal flora furnishes the host with ecological barriers that prevent pathogen attack while maintaining tissue homeostasis. Urinary tract infections (UTIs) constitute a highly relevant model of microbial adaptation in which some patients infected with Escherichia coli develop acute pyelonephritis, while other patients with bacteriuria exhibit an asymptomatic carrier state similar to bacterial commensalism. It remains unclear if the lack of destructive inflammation merely reflects low virulence or if carrier strains actively inhibit disease-associated responses in the host. Here, we identify a new mechanism of bacterial adaptation through broad suppression of RNA polymerase II­dependent (Pol II­dependent) host gene expression. Over 60% of all genes were suppressed 24 hours after human inoculation with the prototype asymptomatic bacteriuria (ABU) strain E. coli 83972, and inhibition was verified by infection of human cells. Specific repressors and activators of Pol II­dependent transcription were modified, Pol II phosphorylation was inhibited, and pathogen-specific signaling was suppressed in cell lines and inoculated patients. An increased frequency of strains inhibiting Pol II was epidemiologically verified in ABU and fecal strains compared with acute pyelonephritis, and a Pol II antagonist suppressed the disease-associated host response. These results suggest that by manipulating host gene expression, ABU strains promote tissue integrity while inhibiting pathology. Such bacterial modulation of host gene expression may be essential to sustain asymptomatic bacterial carriage by ensuring that potentially destructive immune activation will not occur.


Assuntos
Bacteriúria/enzimologia , Infecções por Escherichia coli/enzimologia , RNA Polimerase II/metabolismo , Infecções Urinárias/enzimologia , Infecções Assintomáticas , Bacteriúria/imunologia , Bacteriúria/microbiologia , Células Cultivadas , Repressão Enzimática , Células Epiteliais/enzimologia , Células Epiteliais/imunologia , Células Epiteliais/microbiologia , Escherichia coli/imunologia , Escherichia coli/fisiologia , Infecções por Escherichia coli/imunologia , Infecções por Escherichia coli/microbiologia , Fezes/microbiologia , Expressão Gênica , Interações Hospedeiro-Patógeno , Humanos , Imunidade Inata , Fosforilação , Processamento de Proteína Pós-Traducional , Pielonefrite/enzimologia , Pielonefrite/imunologia , Pielonefrite/microbiologia , RNA Polimerase II/genética , Transdução de Sinais , Transcrição Gênica , Infecções Urinárias/imunologia , Infecções Urinárias/microbiologia
18.
Kidney Int ; 83(4): 548-50, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23538695

RESUMO

Antibacterial peptides and proteins maintain the sterility of the human urinary tract. A broad-spectrum antimicrobial protein, ribonuclease 7 (RNase 7), previously discovered to play a role in controlling the growth of bacteria on human skin, has now been shown to have an important antibacterial function in the human urinary tract.


Assuntos
Rim/enzimologia , Pielonefrite/enzimologia , Ribonucleases/metabolismo , Infecções Urinárias/enzimologia , Humanos
19.
Kidney Int ; 83(4): 615-25, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23302724

RESUMO

The mechanisms that maintain sterility in the urinary tract are incompletely understood; however, recent studies stress the importance of antimicrobial peptides in protecting the urinary tract from infection. Ribonuclease 7 (RNase 7), a potent antimicrobial peptide contributing to urinary tract sterility, is expressed by intercalated cells in the renal collecting tubules and is present in the urine at levels sufficient to kill bacteria at baseline. Here, we characterize the expression and function of RNase 7 in the human urinary tract during infection. Both quantitative real-time PCR and enzyme-linked immunosorbant assays demonstrated increases in RNASE7 expression in the kidney along with kidney and urinary RNase 7 peptide concentrations with infection. While immunostaining localized RNase 7 production to the intercalated cells of the collecting tubule during sterility, its expression during pyelonephritis was found to increase throughout the nephron but not in glomeruli or the interstitium. Recombinant RNase 7 exhibited antimicrobial activity against uropathogens at low micromolar concentrations by disrupting the microbial membrane as determined by atomic force microscopy. Thus, RNase 7 expression is increased in the urinary tract with infection and has antibacterial activity against uropathogens at micromolar concentrations.


Assuntos
Rim/enzimologia , Pielonefrite/enzimologia , Ribonucleases/metabolismo , Infecções Urinárias/enzimologia , Bactérias/enzimologia , Estudos de Casos e Controles , Membrana Celular/enzimologia , Ensaio de Imunoadsorção Enzimática , Interações Hospedeiro-Patógeno , Humanos , Rim/microbiologia , Cinética , Viabilidade Microbiana , Microscopia de Força Atômica , Pielonefrite/genética , Pielonefrite/microbiologia , Pielonefrite/urina , RNA Mensageiro/análise , Reação em Cadeia da Polimerase em Tempo Real , Ribonucleases/genética , Ribonucleases/urina , Regulação para Cima , Infecções Urinárias/genética , Infecções Urinárias/microbiologia , Infecções Urinárias/urina
20.
Am J Infect Control ; 41(7): 597-600, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23337303

RESUMO

BACKGROUND: Recently, there has been a notable surge in urinary tract infections (UTIs) by extended spectrum ß-lactamase (ESBL)-producing Escherichia coli, which considerably limits treatment options. This study aimed to determine prevalence, phenotypic patterns, and ESBL-production status of E coli in isolates from UTI patients along with uncovering locally relevant risk factors for contracting ESBL-producing E coli infections. METHODS: One hundred four nonrepetitive urine samples were collected from 3 major hospitals in Aleppo, Syria. Antibiotic susceptibility and ESBL production were studied by disc diffusion and double disk synergy tests according to Clinical Laboratory Standards Institute guidelines. Epidemiologic analysis was done using χ(2) and multivariate logistic regression tests. RESULTS: This study revealed high prevalence of multidrug-resistant (MDR) E coli reaching 63%, whereas ESBL-producing E coli exceeded 52%. The latter exhibited alarmingly elevated levels of coresistance to non-ß-lactam antibiotics leading to vast increase in MDR rates in comparison with non-ESBL-producing E coli (83.6% vs 12.2%, respectively). CONCLUSION: We found previous exposure to third-generation cephalosporins and fluoroquinolones to be a significant risk factor for ESBL-producing E coli infections, in addition to other known factors such as hospitalization and catheterization. Tigecycline and carbapenems demonstrated near perfect efficacy against tested E coli, so they rank high among treatment options.


Assuntos
Antibacterianos/uso terapêutico , Infecções por Escherichia coli/tratamento farmacológico , Infecções por Escherichia coli/enzimologia , Infecções Urinárias/tratamento farmacológico , Infecções Urinárias/enzimologia , Resistência beta-Lactâmica , beta-Lactamases/metabolismo , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Estudos de Coortes , Estudos Transversais , Resistência a Múltiplos Medicamentos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Síria , Adulto Jovem
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