RESUMO
The effect of treatment of Yersinia pseudotuberculosis cells with antibodies of various specificities on adhesiveness of pseudotuberculosis bacteriophage was analyzed by competitive inhibition technique. Bacteriophage adsorption to bacteria was sterically inhibited by monoclonal antibodies to protein epitopes of Y. pseudotuberculosis outer membrane. These results suggest that receptors of pseudotuberculosis diagnostic bacteriophage are localized on the LPS core of microbial cell.
Assuntos
Anticorpos Monoclonais/imunologia , Bacteriófagos/metabolismo , Lipopolissacarídeos/metabolismo , Receptores Virais/imunologia , Ligação Viral , Yersinia pseudotuberculosis/metabolismo , Infecções por Yersinia pseudotuberculosis/virologiaRESUMO
The sample included five indicator pseudotuberculosis strains. The application of these strains permitted to isolate out of 161 strains of Y. pseudotuberculosis 9 bacteriophages identical by their morphologic and serologic characteristics but having individual particularities in their lytic activity. The test on sensitivity to bacteriophages can be used in laboratory diagnostic to differentiate the strains of Yersinia pseudotuberculosis.