RESUMO
Anticoagulants are used to prevent the formation and extension of blood clots in various disorders as prophylactic agents for thrombo-embolic disorders. Designing of specific inhibitors against molecular targets that play a pivotal role in the coagulation cascade is indispensable. Clotting Factor Xa is one such attractive target for the design of new oral anticoagulants because of the unique role factor Xa plays in the coagulation cascade as a connection between the extrinsic and intrinsic pathways. Application of computational techniques in drug discovery process helps in identifying parameters which can lead to achieve better pharmacological profile. The docking interactions and QSAR studies performed on series of 4-methy-3-(6-[phenyl methylene] amino} pyridine-3-yl)-2H chromen-2-one derivatives provide significant insights for designing of better ligands as anticoagulants.
Assuntos
Aminopiridinas/farmacologia , Anticoagulantes/farmacologia , Inibidores dos Fatores de Coagulação Sanguínea/farmacologia , Cumarínicos/farmacologia , Inibidores do Fator Xa , Aminopiridinas/síntese química , Aminopiridinas/química , Anticoagulantes/síntese química , Anticoagulantes/química , Inibidores dos Fatores de Coagulação Sanguínea/síntese química , Inibidores dos Fatores de Coagulação Sanguínea/química , Cumarínicos/síntese química , Cumarínicos/química , Descoberta de Drogas , Modelos Lineares , Modelos Moleculares , Estrutura Molecular , Relação Quantitativa Estrutura-AtividadeRESUMO
We have been researching orally active factor Xa inhibitor for a long time. We explored the new diamine linker using effective ligands to obtain a new attractive original scaffold 2-aminomethylphenylamine derivative. Compound 1D showed very strong in vitro and in vivo factor Xa inhibitory activity, as well as favorable PK profiles in po administration to monkeys.
Assuntos
Compostos de Anilina/química , Compostos de Anilina/síntese química , Inibidores dos Fatores de Coagulação Sanguínea/síntese química , Inibidores do Fator Xa , Animais , Inibidores dos Fatores de Coagulação Sanguínea/química , Cristalografia por Raios X , Haplorrinos , Humanos , Concentração Inibidora 50 , Ligantes , Estrutura Molecular , RatosRESUMO
Highly potent and selective substrate analogue factor Xa inhibitors were obtained by incorporation of non-basic or modestly basic P1 residues known from the development of thrombin inhibitors. The modification of the P2 and P3 amino acids strongly influenced the selectivity and provided potent dual factor Xa and thrombin inhibitors without affecting the fibrinolytic enzymes. Several inhibitors demonstrated excellent anticoagulant efficacy in standard clotting assays in human plasma.
Assuntos
Anticoagulantes/farmacologia , Antitrombinas/farmacologia , Inibidores dos Fatores de Coagulação Sanguínea/farmacologia , Coagulação Sanguínea/efeitos dos fármacos , Inibidores do Fator Xa , Trombina/antagonistas & inibidores , Aminoácidos/metabolismo , Anticoagulantes/síntese química , Antitrombinas/síntese química , Coagulação Sanguínea/fisiologia , Inibidores dos Fatores de Coagulação Sanguínea/síntese química , Humanos , Modelos Químicos , Fragmentos de Peptídeos/metabolismo , Especificidade por SubstratoRESUMO
We found the novel selective and orally available non-amidine TF/FVIIa complex inhibitor 21e, 4-({[(1S)-(aminocarbonyl)-3-methylbutyl]amino}carbonyl)-2'-({[4- (aminomethyl)phenyl]amino}carbonyl)-4'-(methylamino)biphenyl-2- carboxylic acid. The derivatives were synthesized by conversions of the isobutyl moiety and the introduction of alkylamino groups to 4'-position of the central phenyl ring of compounds 2a and 2b reported previously. Some compounds show increased in vitro anti-TF/FVIIa and PT prolongation activities. Among them, compound 21e reached and sustained micromolar plasma concentration levels of up to 2h after oral administration in mice. Moreover, compound 21e did not prolong the bleeding time even at the highest dose level in cynomolgus monkeys, while PT was prolonged 3.7-fold increases at this dose.
Assuntos
Compostos de Bifenilo/síntese química , Compostos de Bifenilo/farmacologia , Inibidores dos Fatores de Coagulação Sanguínea/síntese química , Fator VIIa/antagonistas & inibidores , Lipoproteínas/síntese química , Metilaminas/síntese química , Metilaminas/farmacologia , Tromboplastina/antagonistas & inibidores , Administração Oral , Animais , Sítios de Ligação , Compostos de Bifenilo/química , Inibidores dos Fatores de Coagulação Sanguínea/química , Inibidores dos Fatores de Coagulação Sanguínea/farmacologia , Desenho de Fármacos , Avaliação Pré-Clínica de Medicamentos , Humanos , Ligação de Hidrogênio , Ligantes , Lipoproteínas/química , Lipoproteínas/farmacologia , Macaca fascicularis , Masculino , Metilaminas/química , Camundongos , Camundongos Endogâmicos ICR , Modelos Moleculares , Estrutura Molecular , Estrutura Secundária de Proteína , Sensibilidade e Especificidade , Estereoisomerismo , Relação Estrutura-AtividadeRESUMO
The discovery of a highly potent and selective tissue factor/factor VIIa inhibitor is described. Upon oral administration of its double prodrug in the guinea pig, a dose-dependent antithrombotic effect is observed in an established model of arterial thrombosis without prolonging bleeding time. The pharmacodynamic properties of this selective inhibitor are compared to the behaviour of a mixed factor VIIa/factor Xa inhibitor.