RESUMO
Recently, there has been an increase in the interest in various aspects of the biology of small passerine birds. In order to apply many modern neurobiological techniques, it would be useful to be able to surgically inject hormones and drugs directly into passerine brains. We describe a novel and cost-effective stereotaxic-like technique for injecting substances into the lateral ventricles of small passerine birds. The technique is rapid, reducing the period of anesthesia, and reliable. In addition, using this technique, we injected [3H]cysteine (to label newly synthesized proteins) with small doses of colchicine. Colchicine decreased the content of newly synthesized protein in the median eminence, thus suggesting that colchicine inhibited protein transport. The technique should prove useful in a number of neurobiologial applications.
Assuntos
Aves/fisiologia , Encéfalo/efeitos dos fármacos , Injeções Intraventriculares/instrumentação , Proteínas do Tecido Nervoso/metabolismo , Técnicas Estereotáxicas/instrumentação , Animais , Encéfalo/fisiologia , Mapeamento Encefálico/instrumentação , Colchicina/farmacologia , Análise Custo-Benefício , Injeções Intraventriculares/economia , Eminência Mediana/efeitos dos fármacos , Eminência Mediana/fisiologia , Técnicas Estereotáxicas/economiaRESUMO
An inexpensive and simple method to fabricate intracerebroventricular (i.c.v.) cannulae for rats with readily available material is described here. The procedure is cost effective and quick. The cannulae thus designed are suitable for injecting minute quantities (microliters) of drugs/chemicals interacerebroventricularly for acute or chronic experiments in rats.
