RESUMO
The most serious problem usually encountered in the field of implanted biomedical devices is infectious morbidity as a primary source of mortality. In this work, the synthesis and characterization of a macroporous iodine-based sanitizer (iodophor), poly(caprolactone)-iodine (PCL-I(2)), are presented. Characterization methods include nuclear magnetic resonance spectroscopy, gel permeation chromatography, nitrogen adsorption-desorption, and scanning electron microscopy. The in vitro cytotoxicity to CHO cells based on cell viability with Chinese hamster ovary cells (CHO) and antimicrobial activities against Escherichia coli and Staphylococcus aureus were examined. The obtained macropore PCL-I(2) structures had a rather narrow size distribution. The PCL-I(2) iodophor was noncytotoxic to Chinese hamster ovary cells. The antimicrobial activities of the PCL-I(2) were assessed against E. coli and S. aureus. The tested PCL-I(2) showed better antimicrobial activity against E. coli than against S. aureus.
Assuntos
Antibacterianos/farmacologia , Materiais Biocompatíveis/síntese química , Materiais Biocompatíveis/farmacologia , Iodóforos/farmacologia , Poliésteres , Animais , Antibacterianos/síntese química , Células CHO , Sobrevivência Celular/efeitos dos fármacos , Cricetinae , Desinfetantes/síntese química , Desinfetantes/farmacologia , Escherichia coli/efeitos dos fármacos , Implantes Experimentais , Iodo/química , Iodóforos/síntese química , Teste de Materiais , Poliésteres/síntese química , Poliésteres/farmacologia , Porosidade , Staphylococcus aureus/efeitos dos fármacosRESUMO
Synthetic calcitonin (CT) and Tyr(0)-katacalcin (tKT) were radioiodinated with Iodogen to high specific activity and with high yields. The products of the iodination procedure were chromatographed on Sephadex G-25 to remove unreacted iodide and then separated by chromatofocussing on PBE 94 (pH 9.6-6.0 for CT and pH 7.4-4.0 for tKT). Clear separation between uniodinated peptides and their mono- and di-iodinated derivatives was achieved with specific activities of 1900 and 3800 Ci/mmol for the respective mono- and di-iodinated peptides. Yields were up to 36 and 24% of mono- and di-iodinated CT and 41 and 29% for mono- and di-iodinated tKT. Our results show that Iodogen provides an effective and gentle way to iodinate peptides with high efficiency. Chromatofocussing is a simple, inexpensive and instrumentally undemanding method that can be performed without specialized chromatographic equipment and that should be applicable to a variety of different tracer preparations.
Assuntos
Calcitonina/metabolismo , Iodo/síntese química , Iodóforos/síntese química , Compostos de Tosil , Ureia/análogos & derivados , Calcitonina/síntese química , Cloraminas , Cromatografia em Gel , Lactoperoxidase , Fragmentos de Peptídeos/metabolismo , Peptídeos/isolamento & purificação , Ureia/metabolismoRESUMO
Five nonionic tensides of a general formula 4-R-C6H4O(CH2CH2O)nCH2CH2OH were prepared by modifying in their structure the nature of the substituent in phenyl group and the length of the ethyleneglycol polycondensate linked with the oxygen of the phenol rest. From the tensides obtained in this way appropriate complexes of iodophorous character were prepared by treating them with iodine solution in hydroiodic acid. Using 131I it was possible to obtain complexes labelled with 131I. This in turn enabled us to determine the degree of iodine complexing in the iodophor solutions by means of a radiometric method.