Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 6 de 6
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Spectrochim Acta A Mol Biomol Spectrosc ; 227: 117542, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31685427

RESUMO

Novel boronate affinity imprinted quantum dots (BA-CdTe@MIPs QDs) were used to develop a selective and sensitive fluorescent nanosensor for determination of cis-diol-containing flavonoids such as quercetin (Qu), baicalein (Bai) and luteolin (Lut) based on controllable oriented surface imprinting approach. The boronate affinity imprinted silica was used as recognition elements. Under the optimum conditions, the imprinting factor (IF) for Qu, Bai and Lut was evaluated to be 9.42, 6.58 and 10.91, respectively. The results indicated that the boronate affinity quantum dots coated with imprinted silica were successfully prepared. The obtained BA-CdTe@MIPs QDs provided high selectivity and high sensitivity for cis-diol-containing flavonoids such as quercetin and luteolin. The BA-CdTe@MIPs QDs exhibited linear decrease in fluorescence intensity with the increase of concentration of quercetin in the 0.05-25 µM concentration range. The detection limit (LOD) is evaluated to be 0.02 µM. The obtained fluorescent nanosensor could be successfully applied to efficient detection of cis-diol-containing flavonoids in onion skin and human urine samples. The recoveries for the spiked onion skin and urine samples were evaluated to be 83.50-104.00% and 86.67-105.00%, respectively. Clearly, this study provides a rapid and efficient fluorescent detection tool for cis-diol-containing flavonoids in real samples.


Assuntos
Ácidos Borônicos/química , Flavonoides/análise , Flavonoides/urina , Pontos Quânticos/química , Dióxido de Silício/química , Compostos de Cádmio/química , Flavanonas/análise , Flavanonas/urina , Humanos , Limite de Detecção , Luteolina/análise , Luteolina/urina , Masculino , Impressão Molecular/métodos , Cebolas/química , Quercetina/análise , Quercetina/urina , Espectrometria de Fluorescência/métodos , Telúrio/química
2.
Mol Nutr Food Res ; 57(5): 877-85, 2013 May.
Artigo em Inglês | MEDLINE | ID: mdl-23386290

RESUMO

SCOPE: The study aimed to investigate the regioselectivity of methylation of luteolin (3',4',5,7-tetrahydroxyflavone) in human in vitro and in vivo. METHODS AND RESULTS: Recombinant human catechol-O-methyltransferase (COMT) and human liver S9 were utilized to study the kinetics of meta (3')- and para (4')- methylation of luteolin, and urine samples from volunteers after giving a luteolin-containing formulation were collected to determine the ratio of para-/meta-production. The results showed luteolin favored a para-methylation, with a ratio of of para-/meta-production in CLint (1.43 in recombinant human COMT and 1.47 in human liver S9), which was contrary to the known substrates of COMT. However, the result of urine sample assay showed a preference of meta-methylation with a ratio of of para-/meta-production (0.460 ± 0.126). To elucidate the mechanism for different preference of methylation of luteolin in vitro and in vivo, metabolism stability of the meta- and para-methylated luteolin was evaluated in human liver microsomes and recombinant human CYP450s, which revealed that para-methylated luteolin was more easily demethylated by human CYP1A2 and CYP3A4/5 than meta-methylated luteolin. CONCLUSION: Luteolin was a rare substrate of human COMT favoring a para-methylation, but further demethylation by human CYP1A2 and CYP3A4/5 caused a preference of accumulation in meta-methylated luteolin in vivo.


Assuntos
Catecol O-Metiltransferase/metabolismo , Luteolina/farmacologia , Administração Oral , Adulto , Citocromo P-450 CYP1A2/metabolismo , Citocromo P-450 CYP3A/metabolismo , Feminino , Humanos , Fígado/metabolismo , Luteolina/urina , Masculino , Metilação , Microssomos Hepáticos/metabolismo , Proteínas Recombinantes
3.
J Agric Food Chem ; 60(33): 8123-8, 2012 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-22812915

RESUMO

5,7,3',4'-Tetramethoxyflavone (TMF), one of the major polymethoxyflavones (PMFs) isolated from Kaempferia parviflor , has been reported possessing various bioactivities, including antifungal, antimalarial, antimycobacterial, and anti-inflammatory activities. Although several studies on the TMF have been reported, the information about the metabolism of TMF and the structures of TMF metabolites is still not yet clear. In this study, an isotope-labeling method was developed for the identification of TMF metabolites. Three isotope-labeled TMFs (5,7,3',4'-tetramethoxy[3'-D(3)]flavone, 5,7,3',4'-tetramethoxy[4'-D(3)]flavone, and 5,7,3',4'-tetramethoxy[5,4'-D(6)]flavone) were synthesized and administered to rats. The urine samples were collected, and the main metabolites were monitored by ultrahigh-performance liquid chromatography-electrospray ionization-mass spectrometry. Five TMF metabolites were unambiguously identified as 3'-hydroxy-5,7,4'-trimethoxyflavone, 7-hydroxy-5,3',4'-trimethoxyflavone sulfate, 7-hydroxy-5,3',4'-trimethoxyflavone, 4'-hydroxy-5,7,3'-trimethoxyflavone, and 5-hydroxy-7,3',4'-trimethoxyflavone.


Assuntos
Cromatografia Líquida/métodos , Marcação por Isótopo/métodos , Luteolina/urina , Espectrometria de Massas por Ionização por Electrospray/métodos , Animais , Luteolina/metabolismo , Masculino , Ratos , Ratos Sprague-Dawley
4.
Drug Metab Dispos ; 39(4): 667-74, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21209248

RESUMO

Luteolin is mainly metabolized by phase II enzymes in animals and humans with glucuronidation and sulfation as the two known metabolic pathways. Although methylation of luteolin was reported previously, the structure of the methylated metabolites and the enzymes involved in the process have not been clarified. In our study, two methylated metabolites, M1 (chrysoeriol) and M2 (diosmetin), were identified in the urine after intravenous administration of luteolin to rats, and the data suggested that the methylation was mediated by catechol-O-methyltransferase (COMT). When luteolin was coadministered with a specific COMT inhibitor, entacapone, the formation of M1 and M2 was significantly reduced, whereas the plasma concentration of luteolin increased. Methylation of luteolin was also studied in vitro using rat tissue homogenates. The apparent kinetic parameters associated with the formation of M1 and M2 in vitro were estimated, and regioselectivity of methylation of luteolin was observed. In the in vitro experiment, there was a preference for the formation of M2 over M1. In contrast, accumulation of M1 was preferred in vivo in both rat plasma and urine after an intravenous dose of luteolin. In conclusion, COMT played a crucial role in the disposition of luteolin in rats. Our results indicated that the methylation pathway in rats was significantly reduced when luteolin was coadministered with a specific COMT inhibitor. Therefore, COMT-associated drug-drug interactions need be considered in the future in luteolin clinical trials because the plasma concentrations and related therapeutic effects may be altered in vivo in the presence of a COMT inhibitor.


Assuntos
Anti-Inflamatórios/farmacocinética , Antiparkinsonianos/farmacocinética , Catecol O-Metiltransferase/metabolismo , Luteolina/farmacocinética , Animais , Anti-Inflamatórios/sangue , Anti-Inflamatórios/metabolismo , Antiparkinsonianos/metabolismo , Catecóis/metabolismo , Catecóis/farmacocinética , Luteolina/sangue , Luteolina/metabolismo , Luteolina/urina , Masculino , Desintoxicação Metabólica Fase I , Metilação , Microssomos Hepáticos/metabolismo , Nitrilas/metabolismo , Nitrilas/farmacocinética , Ratos , Ratos Sprague-Dawley
5.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 37(2): 139-45, 2008 03.
Artigo em Chinês | MEDLINE | ID: mdl-18422272

RESUMO

OBJECTIVE: To observe the excretion of luteolin after oral administration of Elsholtzia blanda benth extracts in rats. METHODS: Samples of urine, feces and bile were collected after oral administration of Elsholtzia blanda benth extracts in rate. After deconjugation with beta-glucuronidase/sulfatase, the levels of luteolin in urine, feces and bile were measured by RP-HPLC. RESULT: The recovery rate of luteolin was 98.0 %-106.0 % and the extract recoveries were 85.0 %-108.0%. Relative standard deviation (RSD) of intra-and inter-day assay was less than 10.0 %. The total accumulative excretion was 37 % (11 % in urine, 26 % in feces and bile). CONCLUSION: The established RP-HPLC method is sensitive, specific, accurate, and is applicable for determination of luteolin in rat urine,feces and bile.


Assuntos
Lamiaceae/química , Luteolina/farmacocinética , Administração Oral , Animais , Bile/química , Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/metabolismo , Medicamentos de Ervas Chinesas/farmacocinética , Fezes/química , Luteolina/isolamento & purificação , Luteolina/urina , Masculino , Extratos Vegetais/administração & dosagem , Ratos , Ratos Sprague-Dawley
6.
J Pharm Biomed Anal ; 41(1): 261-5, 2006 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-16318906

RESUMO

A simple, selective, precise, and accurate RP-HPLC assay for simultaneous analysis of luteolin and apigenin in human urine was developed and validated. Prior to HPLC analysis, urine samples were incubated with beta-glucuronidase/sulfatase. Separation and quantification were achieved on an Agilent C18 column under isocratic conditions using a mobile phase (methanol:0.2% phosphoric acid aqueous solution 55:45, v/v) maintained at 1.0 ml/min at 30 degrees C. The standard curves were linear over the range of 0.0975-7.800 and 0.1744-13.95 microg/ml for luteolin and apigenin, respectively (r > 0.999). The assay recoveries for luteolin and apigenin were above 85.7%. The intra-day and inter-day precision (R.S.D.) for luteolin were below 2.2 and 4.0%, respectively, and for apigenin were less than 2.8 and 5.4%, respectively. Stability studies showed three concentration of luteolin and apigenin in urine quality control samples were stable undergoing three freeze-thaw cycles, storage at room temperature for 4 h, and at -20 degrees C for 3 days. The limit of quantitation was 39.20 ng/ml (n = 5) for luteolin and 31.45 ng/ml (n = 5) for apigenin in human urine. The method developed was employed successfully to determine luteolin and apigenin in urine samples obtained from eight healthy volunteers following oral administration of tablet of Chrysanthemum morifolium extract (CME).


Assuntos
Apigenina/urina , Cromatografia Líquida de Alta Pressão/métodos , Chrysanthemum/metabolismo , Luteolina/urina , Administração Oral , Apigenina/administração & dosagem , Apigenina/análise , Calibragem , Cromatografia/métodos , Humanos , Luteolina/administração & dosagem , Luteolina/análise , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Comprimidos , Fatores de Tempo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...