The Effects of Two Different Burn Dressings on Serum Oxidative Stress Indicators in Children with Partial Burn.

In this study, we evaluated and compared the effect of treatment with a hydrofiber dressing with silver (HFAg) and a polylactic membrane (PLM) on systemic oxidative stress in systemic inflammatory reaction in thermal burn injuries in children. A prospective randomized and matched pairing study of 20 to 50% of TBSA was performed from children equal to both sexes affected by thermal injuries. The control group was included in normal children of both sexes. Serum malondialdehyde (MDA), total antioxidant capacity (TAC), total oxidant capacity (TOC), and glutathione (GSH) levels were analyzed and the results were analyzed statistically. In this study, it was found that PLM treatment increased TAC and GSH levels in burn patients significantly more than the other group. With the use of PLM, TOC decreased to normal level from day 3. In the HFAg group, TAC and GSH levels began to increase on the seventh day. On the first day of the burn, the TOC level started to increase. This increase continued on days 7 and 14. The TOC level began to fall on the 21st day. The increase in TAC was higher in the PLM group. In the PLM group, TOC fell faster. As a result, we think that different burn dressings can have different systemic effects. We can speculate that PLM has an antioxidant effect in the burn tissue due to high lactate content. Therefore, PLM may have decreased serum oxidative stress indicators more effectively than HFAg.

Malondialdehído en la fase inicial de la pancreatitis aguda / Malondialdehyde in early phase of acute pancreatitis

Objetivos: valorar el estrés oxidativo en la pancreatitis aguda, su evolución a lo largo del tiempo y su relación con la gravedad de la enfermedad. Métodos: durante un periodo de dos años, se estudiaron los pacientes ingresados por pancreatitis aguda con dolor abdominal de menos de 24 horas de evolución. Se obtuvo suero de los pacientes el primer, segundo y cuarto día de ingreso, en el momento en que se detectaban complicaciones y tras la recuperación. La concentración de malondialdehído fue determinada utilizando cromatografía líquida de alta resolución. Veinte voluntarios sanos conformaron el grupo control. La concentración de malondialdehído entre los diferentes grupos se comparó utilizando el test de Mann-Whitney y el test de Kruskal-Wallis; la evolución de malondialdehído se valoró mediante el test de Wilcoxon. Resultados: se incluyeron 169 pacientes (91 mujeres, edad mediana 67 años, rango 20-95); 33 sufrieron un episodio grave. La concentración de malondialdehído descendió desde el primer al cuarto día (0,600 vs. 0,451 vs. 0,343 M, respectivamente, p < 0,05). Cuando se detectaban complicaciones, los niveles de malondialdehído eran similares a los del primer y segundo día (0,473 μM, p > 0,05). En los episodios graves la concentración de malondialdehído fue superior que en el grupo control en el día 2 (grave: 0,514; leve: 0,440; control: 0.347 μM, p < 0,05 grave vs. control). Conclusiones: en la pancreatitis aguda existe un estrés oxidativo precoz. En los episodios graves, el estrés oxidativo permanece elevado durante más tiempo que en los episodios leves. El desarrollo de complicaciones se asocia a una elevada concentración de malondialdehído(AU)

Aims: to assess oxidative stress in acute pancreatitis, its evolution over time and its relationship with the severity of the disease. Methods: during a two-year period, patients with acute pancreatitis with less than 24 hours of pain were evaluated. Serum was obtained the first, second and fourth day from admittance, if complications were detected, and after recovery. Malondialdehyde was determined by high performance liquid chromatography. Twenty healthy volunteers constituted the control group. Malondialdehyde between groups was compared with Mann-Whitney and Kruskal-Wallis tests; malondialdehyde evolution was studied with Wilcoxon test. Results: one hundred and sixty-nine patients were included (91 women, median age 67 years, range 20-95); 33 suffered a severe episode. Malondialdehyde decreased from first to fourth day (0.600 vs. 0.451 vs. 0.343 μM, respectively, p < 0.05). When complications were detected, malondialdehyde level was similar to that of first and second day (0.473 M, p > 0.05). In severe attacks malondialdehyde was higher than in control group at day 2 (severe: 0.514; mild: 0.440; control: 0.347 μM, p < 0.05 severe vs. control). Conclusions: an early oxidative stress is observed in acute pancreatitis. In severe attacks, oxidative stress remains high longer than in mild episodes. The onset of complications is associated with high malondialdehyde concentration(AU)

Antioxidant therapy in idiopathic tinnitus: preliminary outcomes.

BACKGROUND: Reactive oxygen species (ROS) play an important role in several pathogenic processes, damaging various structural and functional cellular components. The endothelium is at major risk of radical-induced lesions and this damage is most manifest in microcirculation. It has been recently observed that ROS are implicated in the pathology of the inner ear and the peripheral and central pathways. In a previous study we detected high serum values of ROS in subjects with idiopathic tinnitus. The purpose of the present study was to evaluate the validity of antioxidant treatment in tinnitus sufferers with high ROS values. METHODS: The study considered 31 consecutive patients with unilateral idiopathic tinnitus. The mean pure tone audiometric threshold (PTA), tinnitus loudness, subjective disturbance level [visual analogue scale (VAS) determination], and the indirect ROS dosage 48 h before and after medical treatment were evaluated. Patients underwent an 18-week oral treatment with a mix of phospholipids and vitamins (glycerophosphorylcholine, glycerophosphorylethanolamine, beta-carotene, vitamin C, vitamin E). RESULTS: ROS levels were significantly reduced following antioxidant treatment (malonaldehyde: 2.10 vs. 1.98 mumol/dL, p = 0.003; 4-hydroxynonenal: 2.36 vs. 2.16 mumol/dL, p = 0.002) In addition, great improvement was observed in the reduction of tinnitus (VAS and tinnitus loudness evaluations). No significant changes in audiometric threshold occurred. CONCLUSIONS: Oral antioxidant therapy in patients with idiopathic tinnitus seems to reduce the subjective discomfort and tinnitus intensity and may be considered as an additional treatment modality.

Concentraciones de malondialdehido en un grupo de diabéticos tipo 1 con retinopatía diabética / Malondialdehyde concentrations in a group of type 1 diabetics with diabetic retinopathy

Antecedentes y objetivos: Se realizó un estudio transversal en 70 diabéticos tipo l(35 sin retinopatía diabética (RD) y 35 con RD) con el objetivo de determinar la relación existente entre las concentraciones de malondialdehido (MDA) con la presencia y gravedad de la RD. Material y métodos: A todos los pacientes se les determinó en plasma: glucosa, creatinina, hemoglobina glicosilada (Hb A1), y MDA. También se determinó la excreción urinaria de albúmina (EUA). Resultados: Los niveles de EUA fueron significativamente mayores en los afectados de RD al compararlos con los de los pacientes sin esta complicación (99,1 ñ 129,8 mg/L vs 34,3 ñ 69,8 mg/L. Las concentraciones de MDA fueron significativamente mayores (p<0,01) en los pacientes portadores de RD al compararlos con los que no la tenían (863,4ñ 348,0 nmol/L vs 183,5 ñ 435,0 nmol/L) y significativamente mayores en aquellos con RD proliferativa al compararlos con los valores de los afectados de RD no proliferativa (1196,2 ñ 398,1 nmol/L vs 914,4 ñ 448,2 nmol/L). Conclusiones: comprobamos en nuestro estudio que en los pacientes con DM tipo 1 existe un estrés oxidativo, expresado por concentraciones elevadas de MDA, significativamente mayor en los portadores de RD, el cual se incrementa con la severidad de esta complicación. Los niveles de MDA constituyen un buen marcador del estrés oxidativo en los pacientes portadores de RD. (AU)

Serum malondialdehyde: possible use for the clinical management of chronic hepatitis C patients.

Serum lipid peroxidation products are increased in inflammatory liver disease and, as we previously reported, also in chronic hepatitis C. We have performed a specific assay of malondialdehyde, the reported most abundant product of lipid peroxidation, in serum of twenty four chronic hepatitis C patients, before, during, and after interferon treatment. Liver biopsies were performed in each patient before and after interferon treatment. The results show higher serum malondialdehyde values in chronic hepatitis C patients than healthy subjects (n = 68) before interferon treatment (p < .001). Mean value of serum malondialdehyde levels after interferon treatment was significantly lower than before it (p < .002). Associating the histopathological findings in each of the 48 biopsies performed, with serum malondialdehyde and alanine aminotransferase activity levels, of the sample obtained the same day of biopsy, a much better correspondence with the histopathological severity was observed for malondialdehyde concentration than for alanine aminotransferase activity. These levels decreased significantly after interferon treatment. However, when the patients were grouped in responding (group I; n = 9) and non-responding (group II; n = 15) to interferon treatment, according to the histopathological findings before and after interferon, the values of group I before interferon treatment were significantly higher than group II (p < .03). Thus, a potential predictive value could be ascribed to the serum malondialdehyde levels before interferon treatment in these patients. We propose the utility of the specific assay of malondialdehyde for the clinical management of chronic hepatitis C patients.

[Free radical scavenging agents in myocardial protection during cardiac surgery]. / Les piégeurs de radicaux libres dans la protection myocardique en chirurgie cardiaque.

Oxygen free radicals are very unstable metabolites which are produced in abundant quantity during the reoxygenation of an ischemic organ. Oxidation, by these radicals, of the structural lipids of the membranes, is at the origin of cellular lesions all the more extensive as the ischemia, by itself, decreases the ischemic tissue content in "trapping" molecules which usually inactivate those free radicals. Thus, was introduced the concept of an exogenous supply of trappers intended to bring under control the production of radicals and consequently preserve the membrane integrity in the revascularized tissue. This review summarizes, in light of our experience, the results obtained with free radicals trappers in the scope of myocardial preservation, especially in cardiac surgery, and analyzes some of the problems that remain to be resolved before considering the clinical use of these trappers.