Biomechanical and chemical behavior of various bioactive materials in class II restorations / Desempeño biomecánico y químico de diferentes materiales bioactivos en restauraciones clase II

The aim of this in vitro study was to evaluate the biomechanical and chemical behavior of various bioactive materials in class II MOD restorations. Forty- eight standardized class II MOD cavities were prepared in sound extracted human molar teeth. The specimens were divided into four groups according to the restorative material: Group 1 (Filtek™ Z350 XT), Group 2 (Biodentine™ as a liner, and then restored with Filtek™ Z350 XT), Group 3 (Cention N™), and Group 4 (Activa™ Bioactive-Restorative). The samples were tested for fracture resistance by subjecting them to a compressive load in a Universal testing Machine. The failure modes of each specimen were evaluated. The alkalinizing potential and calcium ion release of the materials were measured. SEM-EDAX analyses were also performed for all materials. Data were analyzed using ANOVA and post hoc Tukey test (p<0.05). Group 1 showed the higher fracture resistance (p<0.05). Group 3 had greater fracture resistance values but no statistical difference from Group 4. Biodentine™ showed greater biomineralization potential. Class II MOD restorations of Group 1 displayed the higher fracture resistance; however, it was highly associated with catastrophic failure. Conversely, Biodentine™ presented a more significant bioactivity potential, and its use, as in Group 2, promoted the most favorable failure mode.

El objetivo de este estudio, in vitro, fue evaluar el desempeño biomecánico y químico de varios materiales bioactivos en restauraciones clase II MOD. Se prepararon cuarenta y ocho cavidades clase II MOD estandarizadas en dientes molares humanos extraídos. Las muestras se dividieron en cuatro grupos según el material de restauración: Grupo 1 (Filtek™ Z350 XT), Grupo 2 (Biodentine™ como base y luego restaurado con Filtek™ Z350 XT), Grupo 3 (Cention N™) y Grupo 4 (Activa™ Bioactivo-Reparador). La prueba de resistencia a la fractura fue realizada en una máquina de ensayo universal Instron. Se evaluaron los modos de falla de cada espécimen. Se midió el pH y la liberación de iones de calcio de los materiales. Se realizaron análisis SEM-EDAX. Los datos se analizaron mediante ANOVA y la prueba post hoc de Tukey (p<0,05). El grupo 1 mostró la mayor resistencia a la fractura (p<0,05). El Grupo 3 tuvo mayores valores de resistencia a la fractura que el Grupo 4, pero sin diferencia estadística. Biodentine™ mostró un mayor potencial de biomineralización. Las restauraciones Clase II MOD del Grupo 1 mostraron la mayor resistencia a la fractura; sin embargo, estuvo altamente asociado con fallas irreparables. Por el contrario, Biodentine™ presentó un potencial de bioactividad más significativo y su uso, como en el Grupo 2, promovió el modo de falla más favorable.

The bioactivity and ion release of titanium-containing glass polyalkenoate cements for medical applications.

The ion release profiles and bioactivity of a series of Ti containing glass polyalkenoate cements. Characterization revealed each material to be amorphous with a T(g) in the region of 650-660°C. The network connectivity decreased (1.83-1.35) with the addition of TiO(2) which was also evident with analysis by X-ray photoelectron spectroscopy. Ion release from cements were determined using atomic absorption spectroscopy for zinc (Zn(2+)), calcium (Ca(2+)), strontium (Sr(2+)), Silica (Si(4+)) and titanium (Ti(4+)). Ions such as Zn(2+) (0.1-2.0 mg/l), Ca(2+) (2.0-8.3 mg/l,) Sr(2+) (0.1-3.9 mg/l), and Si(4+) (14-90 mg/l) were tested over 1-30 days. No Ti(4+) release was detected. Simulated body fluid revealed a CaP surface layer on each cement while cell culture testing of cement liquid extracts with TW-Z (5 mol% TiO(2)) produced the highest cell viability (161%) after 30 days. Direct contact testing of discs resulted in a decrease in cell viability of the each cement tested.

Novel stir bar sorptive extraction methods for environmental and biomedical analysis.

Stir bar sorptive extraction (SBSE) is sample preparation technique that involves the extraction and enrichment of organic compounds from a liquid sample. The technique is based on the principle of sorptive extraction. A large amount of extraction phase is coated on a stir bar. An analyte is extracted into the extraction phase, based on its octanol-water partitioning coefficient and the phase ratio. Recently, various methods involving SBSE were developed in order to further facilitate analysis and improve sensitivity. In this review, we focused on the novel methods that involve SBSE with in situ derivatization, SBSE with in situ de-conjugation, thermal desorption (TD) in the multi-shot mode and TD with in tube derivatization method. Those methods were applied successfully to the trace analysis of environmental and biological samples and extremely low detection limits were achieved.