RESUMO
Hydroxytyrosol and two other polyphenols of olive tree, hydroxytyrosol acetate and 3,4-dihydroxyphenylglycol, are known for a wide range of beneficial activities in human health and prevention from diseases. The inability to isolate high, pure amounts of these natural compounds and the difficult and laborious procedures for the synthesis of them led us to describe herein an efficient, easy, cheap, and scaling up synthetic procedure, from catechol, via microwave irradiation.
Assuntos
Técnicas de Química Sintética , Metoxi-Hidroxifenilglicol/análogos & derivados , Álcool Feniletílico/análogos & derivados , Técnicas de Química Sintética/métodos , Humanos , Metoxi-Hidroxifenilglicol/síntese química , Metoxi-Hidroxifenilglicol/química , Estrutura Molecular , Álcool Feniletílico/síntese química , Álcool Feniletílico/químicaRESUMO
The concentrations of free and total 3-methoxy-4-hydroxyphenylethylene glycol (MHPG) in the cerebrospinal fluid of 43 patients were measured by selected ion monitoring gas chromatography-mass spectrometry using MHPG specifically labelled with deuterium as internal standard. Unpaired Wilcoxon comparisons of free and total MHPG concentrations in males and females showed no significant difference between the sexes. The relationships between free, conjugated and total MHPG concentrations and age, blood pressure, glucose and protein levels in cerebrospinal fluid were examined by linear regression analysis. The only significant correlations found were between free MHPG and glucose and between total MHPG and glucose.
Assuntos
Pressão Sanguínea , Glicóis/líquido cefalorraquidiano , Hipertensão/líquido cefalorraquidiano , Transtornos Mentais/líquido cefalorraquidiano , Metoxi-Hidroxifenilglicol/líquido cefalorraquidiano , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Feminino , Glucose/líquido cefalorraquidiano , Humanos , Hipertensão/fisiopatologia , Lactente , Masculino , Transtornos Mentais/fisiopatologia , Metoxi-Hidroxifenilglicol/síntese química , Pessoa de Meia-IdadeRESUMO
We present the first published procedure for the measurement of endogenous conjugated 3,4-dihydroxyphenylglycol (DOPEG) in the rat brain. Conjugated DOPEG is estimated from brain extracts after enzymic hydrolysis, isolation of hydrolysed DOPEG on alumina, methylation of DOPEG to 3-methoxy-4-hydroxyphenylglycol (MOPEG) and gas chromatographic quantification of MOPEG. The level of conjugated DOPEG in the CNS of rats (65.7 +/- 0.7 ng/g whole brain tissue corrected for recovery) almost equals the level of conjugated MOPEG. The sensitivity of the method is about 6 ng/g brain tissue. After inhibition of monoamine oxidase with clorgyline (30 mg/kg) conjugated DOPEG and MOPEG both disappeared from the brain with a half-life of about 1 h. Turnover calculations indicate that conjugated DOPEG and MOPEG are the two major noradrenaline end-metabolites in the rat brain. The method of estimating conjugated DOPEG also allows the measurement of noradrenaline, dopamine and total MOPEG in an extract from one half of a rat brain.
