RESUMO
Inulin-type oligosaccharides with different DP were prepared by size-exclusion chromatography and purity of each oligosaccharide was determined by HPLC equipped with cyclodextrin-bond column. The purities of obtained inulin-type oligosaccharides with different DP were more than 98% by one-step process. The DP and molecular weight were obtained through ESI-MS in negative mode. The characterization of the inulin-type oligosaccharides with different DP was studied by MS/MS spectra obtained by collision-induced dissociation of molecular ions ([M-H](-)). When the DP was lower, the fragment ions were formed through cross-ring cleavages of two bonds within the sugar ring and glycosidic cleavages. However, with the increase of DP, the ions resulting from glycosidic cleavages between two sugar residues were predominant.
Assuntos
Inulina/química , Medicina Tradicional Chinesa , Morinda/química , Oligossacarídeos/isolamento & purificação , Espectrometria de Massas por Ionização por Electrospray/métodos , Espectrometria de Massas em Tandem/métodos , Morinda/anatomia & histologia , Oligossacarídeos/química , Raízes de Plantas/químicaRESUMO
OBJECTIVE: To explore the genetic diversity of different farm races of Morinda officinalis on molecular level. METHODS: The molecular biological technique-random amplified polymorphic DNA (RAPD) were used. RESULTS: Of the 40-mer arbitary primers, 14 were found to amplify polymorphic products. 3-5 polymorphic bands were amplified by each polymorphic primer on the average. Using UPGMA method all the tested accesions can be clustered into two groups: 4 accessions of Daye were classified as one group, 1 accession of Xiaoye be another group. CONCLUSION: There actually existed much genetic diversity on molecular level among the different farm races of Morinda officinalis.
Assuntos
Variação Genética , Morinda/genética , Plantas Medicinais/genética , Polimorfismo Genético , Análise por Conglomerados , Primers do DNA , DNA de Plantas/genética , Morinda/anatomia & histologia , Morinda/classificação , Folhas de Planta/anatomia & histologia , Raízes de Plantas/anatomia & histologia , Plantas Medicinais/classificação , Técnica de Amplificação ao Acaso de DNA Polimórfico/métodosRESUMO
The histological structures of the roots of Morinda officinalis How of different ages were observed, the distribution and accumulation of anthraquinones in the root were studied with the help of paraffin section, fluorescent microscope and ultraviolet spectrophotometer. The results indicated that the mature structures of the roots of M. officinalis were similar to those of common perennial herbs, and that the anthraquinones in the root of M. officinalis distributed in parenchymatous cells and the content of anthraquinones in the root gradually increased in number with age. Based on above mentioned investigation, we came to the conclusion that the root of M. officinalis should be collected after four years of growing and the top grade root should have well developed phloem and thin xylem.
