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Front Neural Circuits ; 14: 11, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32296310

RESUMO

Objective: To investigate whether the CSF-contacting nucleus receives brainstem and spinal cord projections and to understand the functional significance of these connections. Methods: The retrograde tracer cholera toxin B subunit (CB) was injected into the CSF-contacting nucleus in Sprague-Dawley rats according the previously reported stereotaxic coordinates. After 7-10 days, these rats were perfused and their brainstem and spinal cord were sliced (thickness, 40 µm) using a freezing microtome. All the sections were subjected to CB immunofluorescence staining. The distribution of CB-positive neuron in different brainstem and spinal cord areas was observed under fluorescence microscope. Results: The retrograde labeled CB-positive neurons were found in the midbrain, pons, medulla oblongata, and spinal cord. Four functional areas including one hundred and twelve sub-regions have projections to the CSF-contacting nucleus. However, the density of CB-positive neuron distribution ranged from sparse to dense. Conclusion: Based on the connectivity patterns of the CSF-contacting nucleus receives anatomical inputs from the brainstem and spinal cord, we preliminarily conclude and summarize that the CSF-contacting nucleus participates in pain, visceral activity, sleep and arousal, emotion, and drug addiction. The present study firstly illustrates the broad projections of the CSF-contacting nucleus from the brainstem and spinal cord, which implies the complicated functions of the nucleus especially for the unique roles of coordination in neural and body fluids regulation.


Assuntos
Tronco Encefálico/química , Líquido Cefalorraquidiano/química , Conectoma/métodos , Imageamento Tridimensional/métodos , Medula Espinal/química , Núcleo do Nervo Abducente/química , Núcleo do Nervo Abducente/citologia , Núcleo do Nervo Abducente/fisiologia , Animais , Tronco Encefálico/citologia , Tronco Encefálico/fisiologia , Aqueduto do Mesencéfalo/química , Aqueduto do Mesencéfalo/citologia , Aqueduto do Mesencéfalo/fisiologia , Líquido Cefalorraquidiano/fisiologia , Vias Neurais/química , Vias Neurais/citologia , Vias Neurais/fisiologia , Ratos , Ratos Sprague-Dawley , Medula Espinal/citologia , Medula Espinal/fisiologia , Núcleos Vestibulares/química , Núcleos Vestibulares/citologia , Núcleos Vestibulares/fisiologia
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