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Anat Histol Embryol ; 43(1): 56-63, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23521715

RESUMO

Distribution pattern of fibre types was studied in the muscles of the soft palate (palatinus, levator veli palatini and tensor veli palatini muscles) in the dog. The fibrillar classification was based on using histochemistry and immunohistochemistry methods: myofibrillar adenosine thriphosphatase (mATPase) to different pH of pre-incubation; nicotine adenine dinucleotide (reduced) tetrazolium reductase (NADH-TR) and finally, application of specific monoclonal antibodies against myosin heavy chain isoforms I, IIa and IIx. In the palatinus and levator veli palatini muscles, pure type I fibres and the hybrid type IIax and IIc were shown, with a checkerboard distribution in the first and a clear predominance of hybrid fibre types (about 98% of the total population) in levator veli palatini muscle. On the other hand, in the tensor veli palatini muscle, type IIx and IIm fibres were identified (fast-twitch fibres related to fast-moving muscles and the powerful jaw muscles of carnivores). The tensor veli palatini muscle had a different distribution and fibrillar composition with predominantly type IIm fibres in its central zone, whilst the peripheral zone was primarily type I and IIx fibres.


Assuntos
Adenosina Trifosfatases/metabolismo , Histocitoquímica/veterinária , Miofibrilas/enzimologia , NADH Tetrazólio Redutase/metabolismo , Palato Mole/anatomia & histologia , Adenosina Trifosfatases/imunologia , Animais , Anticorpos Monoclonais/imunologia , Cães/anatomia & histologia , Feminino , Imuno-Histoquímica/veterinária , Masculino , Miofibrilas/classificação , Cadeias Pesadas de Miosina/imunologia , NADH Tetrazólio Redutase/imunologia , Oxirredução
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