Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 30
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Cell ; 184(16): 4268-4283.e20, 2021 08 05.
Artigo em Inglês | MEDLINE | ID: mdl-34233163

RESUMO

Ultraviolet (UV) light and incompletely understood genetic and epigenetic variations determine skin color. Here we describe an UV- and microphthalmia-associated transcription factor (MITF)-independent mechanism of skin pigmentation. Targeting the mitochondrial redox-regulating enzyme nicotinamide nucleotide transhydrogenase (NNT) resulted in cellular redox changes that affect tyrosinase degradation. These changes regulate melanosome maturation and, consequently, eumelanin levels and pigmentation. Topical application of small-molecule inhibitors yielded skin darkening in human skin, and mice with decreased NNT function displayed increased pigmentation. Additionally, genetic modification of NNT in zebrafish alters melanocytic pigmentation. Analysis of four diverse human cohorts revealed significant associations of skin color, tanning, and sun protection use with various single-nucleotide polymorphisms within NNT. NNT levels were independent of UVB irradiation and redox modulation. Individuals with postinflammatory hyperpigmentation or lentigines displayed decreased skin NNT levels, suggesting an NNT-driven, redox-dependent pigmentation mechanism that can be targeted with NNT-modifying topical drugs for medical and cosmetic purposes.


Assuntos
Fator de Transcrição Associado à Microftalmia/metabolismo , NADP Trans-Hidrogenases/metabolismo , Pigmentação da Pele/efeitos da radiação , Raios Ultravioleta , Animais , Linhagem Celular , Estudos de Coortes , AMP Cíclico/metabolismo , Dano ao DNA , Inibidores Enzimáticos/química , Inibidores Enzimáticos/farmacologia , Predisposição Genética para Doença , Humanos , Melanócitos/efeitos dos fármacos , Melanócitos/metabolismo , Melanossomas/efeitos dos fármacos , Melanossomas/metabolismo , Melanossomas/efeitos da radiação , Camundongos , Camundongos Endogâmicos C57BL , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Monofenol Mono-Oxigenase/genética , Monofenol Mono-Oxigenase/metabolismo , NADP Trans-Hidrogenases/antagonistas & inibidores , Oxirredução/efeitos dos fármacos , Oxirredução/efeitos da radiação , Polimorfismo de Nucleotídeo Único/genética , Complexo de Endopeptidases do Proteassoma/metabolismo , Proteólise/efeitos dos fármacos , Proteólise/efeitos da radiação , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Pigmentação da Pele/efeitos dos fármacos , Pigmentação da Pele/genética , Ubiquitina/metabolismo , Peixe-Zebra
2.
J Parasitol ; 105(2): 321-329, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30998130

RESUMO

The mitochondrial, inner-membrane-associated, reversible NADPH→NAD+ transhydrogenase of the energetically anaerobic adult cestode Hymenolepis diminuta connects NADPH generation, via a mitochondrial NADP+-specific "malic" enzyme, with NADH formation needed for electron transport. In reducing the pyridine nucleotide, the enzyme concomitantly catalyzes transmembrane proton translocation, thereby coupling NADH formation to ATP generation or NADPH formation to ATP hydrolysis. Detergent-solubilized transhydrogenase, from isolated mitochondrial membranes, was purified to apparent homogeneity using ion exchange and hydroxylapatite chromatographies. The enzyme displayed a monomeric Mr of ∼110 kDa and required phospholipid, without which activity was rapidly lost. Of the phospholipids examined, phosphatidylcholine was the most effective. Transhydrogenase-catalyzed NADH formation was inhibited by NAD(P)+ and adenylates, suggesting regulatory effects of the pyridine nucleotides and effects of pyridine nucleotide-simulating molecules. In keeping with its proton-translocating function, the enzyme was inhibited by dicyclohexylcarbodiimide. The isolated enzyme catalyzed neither NADH→NADP+ nor NADH→NAD+ transhydrogenations, thereby suggesting a need for a minimal coupling to electron transport for the NADH→NADP+ reaction as well as enzyme specificity. Anti-transhydrogenase monospecific antibodies proved inhibitory to NADPH→NAD+ transhydrogenation catalyzed by both isolated and membrane-associated enzymes. This purification study apparently represents a first for parasitic helminths or multicellular invertebrates generally and establishes a framework for evaluating the transhydrogenase as a potential site for specific chemotherapeutic attack.


Assuntos
Hymenolepis diminuta/enzimologia , Mitocôndrias/enzimologia , NADP Trans-Hidrogenases/isolamento & purificação , NADP/metabolismo , NAD/metabolismo , Animais , Eletroforese em Gel de Poliacrilamida , Feminino , Immunoblotting , Imunoglobulina G/imunologia , Masculino , NADP Trans-Hidrogenases/antagonistas & inibidores , NADP Trans-Hidrogenases/imunologia , NADP Trans-Hidrogenases/metabolismo , Fosfolipídeos/metabolismo , Fosfolipídeos/farmacologia , Coelhos , Ratos , Ratos Sprague-Dawley
3.
Biomolecules ; 9(2)2019 02 25.
Artigo em Inglês | MEDLINE | ID: mdl-30823587

RESUMO

Circulating fatty acids (FAs) increase with obesity and can drive mitochondrial damage and inflammation. Nicotinamide nucleotide transhydrogenase (NNT) is a mitochondrial protein that positively regulates nicotinamide adenine dinucleotide phosphate (NADPH), a key mediator of energy transduction and redox homeostasis. The role that NNT-regulated bioenergetics play in the inflammatory response of immune cells in obesity is untested. Our objective was to determine how free fatty acids (FFAs) regulate inflammation through impacts on mitochondria and redox homeostasis of peripheral blood mononuclear cells (PBMCs). PBMCs from lean subjects were activated with a T cell-specific stimulus in the presence or absence of generally pro-inflammatory palmitate and/or non-inflammatory oleate. Palmitate decreased immune cell expression of NNT, NADPH, and anti-oxidant glutathione, but increased reactive oxygen and proinflammatory Th17 cytokines. Oleate had no effect on these outcomes. Genetic inhibition of NNT recapitulated the effects of palmitate. PBMCs from obese (BMI >30) compared to lean subjects had lower NNT and glutathione expression, and higher Th17 cytokine expression, none of which were changed by exogenous palmitate. Our data identify NNT as a palmitate-regulated rheostat of redox balance that regulates immune cell function in obesity and suggest that dietary or therapeutic strategies aimed at increasing NNT expression may restore redox balance to ameliorate obesity-associated inflammation.


Assuntos
Ácidos Graxos/farmacologia , Inflamação/tratamento farmacológico , NADP Trans-Hidrogenases/antagonistas & inibidores , Linfócitos T/efeitos dos fármacos , Adulto , Feminino , Humanos , Inflamação/metabolismo , Masculino , Pessoa de Meia-Idade , NADP Trans-Hidrogenases/genética , NADP Trans-Hidrogenases/metabolismo , Linfócitos T/metabolismo
4.
Arch Insect Biochem Physiol ; 69(3): 118-26, 2008 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-18839416

RESUMO

Midgut mitochondria from fifth larval instar Manduca sexta exhibited a transhydrogenase that catalyzes the following reversible reaction: NADPH + NAD(+) <--> NADP(+) + NADH. The NADPH-forming transhydrogenation occurred as a nonenergy- and energy-linked activity. Energy for the latter was derived from the electron transport-dependent utilization of NADH or succinate, or from Mg++-dependent ATP hydrolysis by ATPase. The NADH-forming and all of the NADPH-forming reactions appeared optimal at pH 7.5, were stable to prolonged dialysis, and displayed thermal lability. N,N'-dicyclohexylcarbodiimide (DCCD) inhibited the NADPH --> NAD(+) and energy-linked NADH --> NADP(+) transhydrogenations, but not the nonenergy-linked NADH --> NADP(+) reaction. Oligomycin only inhibited the ATP-dependent energy-linked activity. The NADH-forming, nonenergy-linked NADPH-forming, and the energy-linked NADPH-forming activities were membrane-associated in M. sexta mitochondria. This is the first demonstration of the reversibility of the M. sexta mitochondrial transhydrogenase and, more importantly, the occurrence of nonenergy-linked and energy-linked NADH --> NADP(+) transhydrogenations. The potential relationship of the transhydrogenase to the mitochondrial, NADPH-utilizing ecdysone-20 monooxygenase of M. sexta is considered.


Assuntos
Manduca/enzimologia , NADP Trans-Hidrogenases/metabolismo , Animais , Diálise , Dicicloexilcarbodi-Imida/farmacologia , Trato Gastrointestinal/enzimologia , Temperatura Alta , Concentração de Íons de Hidrogênio , Larva/enzimologia , NADP Trans-Hidrogenases/antagonistas & inibidores , Oligomicinas/farmacologia
5.
Biochemistry ; 42(5): 1217-26, 2003 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-12564924

RESUMO

Transhydrogenase, found in bacterial membranes and inner mitochondrial membranes of animal cells, couples the redox reaction between NAD(H) and NADP(H) to proton translocation. In this work, the invariant Gln132 in the NAD(H)-binding component (dI) of the Rhodospirillum rubrum transhydrogenase was substituted with Asn (to give dI.Q132N). Mixtures of the mutant protein and the NADP(H)-binding component (dIII) of the enzyme readily produced an asymmetric complex, (dI.Q132N)(2)dIII(1). The X-ray structure of the complex revealed specific changes in the interaction between bound nicotinamide nucleotides and the protein at the hydride transfer site. The first-order rate constant of the redox reaction between nucleotides bound to (dI.Q132N)(2)dIII(1) was <1% of that for the wild-type complex, and the deuterium isotope effect was significantly decreased. The nucleotide binding properties of the dI component in the complex were asymmetrically affected by the Gln-to-Asn mutation. In intact, membrane-bound transhydrogenase, the substitution completely abolished all catalytic activity. The results suggest that Gln132 in the wild-type enzyme behaves as a "tether" or a "tie" in the mutual positioning of the (dihydro)nicotinamide rings of NAD(H) and NADP(H) for hydride transfer during the conformational changes that are coupled to the translocation of protons across the membrane. This ensures that hydride transfer is properly gated and does not take place in the absence of proton translocation.


Assuntos
Glutamina/química , NADP Trans-Hidrogenases/química , NAD/química , Prótons , Substituição de Aminoácidos/genética , Asparagina/genética , Proteínas de Bactérias/antagonistas & inibidores , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Sítios de Ligação/genética , Cristalização , Cristalografia por Raios X , Transporte de Elétrons/genética , Glutamina/genética , Cinética , Mutagênese Sítio-Dirigida , NAD/genética , NADP/química , NADP Trans-Hidrogenases/antagonistas & inibidores , NADP Trans-Hidrogenases/genética , Proteínas Recombinantes/antagonistas & inibidores , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Rhodospirillum rubrum/enzimologia , Rhodospirillum rubrum/genética
6.
Biochim Biophys Acta ; 1413(3): 159-71, 1999 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-10556628

RESUMO

Pyridine nucleotide transhydrogenases of bacterial cytosolic membranes and mitochondrial inner membranes are proton pumps in which hydride transfer between NADP(+) and NAD(+) is coupled to proton translocation across cytosolic or mitochondrial membranes. The pyridine nucleotide transhydrogenase of Escherichia coli is composed of two subunits (alpha and beta). Three domains are recognized. The extrinsic cytosolic domain 1 of the amino-terminal region of the alpha subunit bears the NAD(H)-binding site. The NADP(H)-binding site is present in domain 3, the extrinsic cytosolic carboxyl-terminal region of the beta subunit. Domain 2 is composed of the membrane-intrinsic carboxyl-terminal region of the alpha subunit and the membrane-intrinsic amino-terminal region of the beta subunit. Treatment of the transhydrogenase of E. coli with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD chloride) inhibited enzyme activity. Analysis of inhibition revealed that several sites on the enzyme were involved. NBD chloride modified two (betaCys-147 and betaCys-260) of the seven cysteine residues present in the transhydrogenase. Modification of betaCys-260 in domain 2 resulted in inhibition of enzyme activity. Modification of residues other than cysteine residues also resulted in inhibition of transhydrogenation as shown by use of a cysteine-free mutant enzyme. The beta subunit was modified by NBD chloride to a greater extent than the alpha subunit. Reaction of domain 2 and domain 3 was prevented by NADPH. Modification of domain 3 is probably not associated with inhibition of enzyme activity. Modification of domain 2 of the beta subunit resulted in a decreased binding affinity for NADPH at its binding site in domain 3. The product resulting from the reaction of NBD chloride with NADPH was a very effective inhibitor of transhydrogenation. In experiments with NBD chloride in the presence of NADPH it is likely that all of the sites of reaction described above will contribute to the inhibition observed. The NBD-NADPH adduct will likely be more useful than NBD chloride in investigations of the pyridine nucleotide transhydrogenase.


Assuntos
4-Cloro-7-nitrobenzofurazano/farmacologia , Inibidores Enzimáticos/farmacologia , NADP Trans-Hidrogenases/antagonistas & inibidores , Sítios de Ligação , Cisteína/química , Escherichia coli , Membranas Intracelulares/enzimologia , Mutagênese Sítio-Dirigida , Mutação , NADP Trans-Hidrogenases/química , NADP Trans-Hidrogenases/genética , Octoxinol , Plasmídeos , Espectrofotometria Ultravioleta
7.
Biochemistry ; 38(5): 1652-8, 1999 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-9931033

RESUMO

Conserved and semiconserved acidic and basic residues of the beta subunit of the proton-pumping nicotinamide nucleotide transhydrogenase from Escherichia coli potentially involved in proton pumping were investigated. Out of 16 charged residues studied, 6 have not been previously investigated. The most dramatic effects of mutation were observed with beta H91, beta D392, and beta K424. beta H91E showed a pronounced shift of the pH optimum for both reduction of thio-NADP+ by NADH (forward reaction) and reduction of 3-acetylpyridine-NAD+ by NADPH (reverse reaction) to lower pH. This mutant catalyzed a cyclic reduction of 3-acetylpyridine-NAD+ by NADH in the presence of NADP(H) with a pH profile also shifted toward a lower pH. These results are consistent with a mechanism where the normal forward and reverse reactions are indeed limited by protonation/deprotonation of beta H91. The cyclic reaction was affected by mutations of beta H91, probably through conformational changes involving the active NADP(H) site. The beta D392A mutant was inactive with regard to forward and reverse reactions, but showed a wild-type-like pH dependence for the partly active cyclic reaction. However, Km,app for NADP(H) in this reaction was elevated 50-100-fold, suggesting that beta D392 is located in or near the NADP(H)-binding site. Transhydrogenases contain a conserved beta K424-beta R425-beta S426 sequence that has been proposed to be important for NADP(H) binding. beta K424R was strongly inhibited and showed an 18-fold increased Km,app for NADPH in the reverse reaction as compared to wild type. Consequently, this mutation affected all NADP(H)-linked activities and essentially abolished the unspecific interaction of NAD(H) with this site. The pH dependences of the forward and reverse reactions, as well as the cyclic reaction, were shifted to a lower pH as compared to the wild-type enzyme, and the salt dependence was also altered.


Assuntos
Ácido Aspártico/genética , Escherichia coli/enzimologia , Histidina/genética , Lisina/genética , Mutagênese Sítio-Dirigida , NADP Trans-Hidrogenases/genética , Bombas de Próton/genética , Transporte Biológico/genética , Catálise , Sequência Conservada , Ativação Enzimática/genética , Escherichia coli/genética , Ácido Glutâmico/genética , Concentração de Íons de Hidrogênio , Cloreto de Magnésio/farmacologia , NADP/química , NADP Trans-Hidrogenases/antagonistas & inibidores , NADP Trans-Hidrogenases/química , Bombas de Próton/química , Proteínas Recombinantes/antagonistas & inibidores , Proteínas Recombinantes/química , Cloreto de Sódio/farmacologia
8.
Biochim Biophys Acta ; 1231(3): 304-12, 1995 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-7578218

RESUMO

Transhydrogenase from beef-heart mitochondria was solubilised with Triton X-100 and purified by column chromatography. The detergent-dispersed enzyme catalysed the reduction of acetylpyridine adenine dinucleotide (AcPdAD+) by NADH, but only in the presence of NADP+. Experiments showed that this reaction was cyclic; NADP(H), whilst remaining bound to the enzyme, was alternately reduced by NADH and oxidised by AcPdAD+. A period of incubation of the enzyme with NADPH at pH 6.0 led to inhibition of the simple transhydrogenation reaction between AcPdAD+ and NADPH. However, after such treatment, transhydrogenase acquired the ability to catalyse the (NADPH-dependent) reduction of AcPdAD+ by NADH. It is suggested that this is a similar cycle to the one described above. Evidently, the binding affinity for NADP+ increases as a consequence of the inhibition process resulting from prolonged incubation with NADPH. The pH dependences of simple and cyclic transhydrogenation reactions are described. Though more complex than those in Escherichia coli transhydrogenase, they are consistent with the view [Hutton, M., Day, J.M., Bizouarn, T. and Jackson, J.B. (1994) Eur. J. Biochem. 219, 1041-1051] that, also in the mitochondrial enzyme, binding and release of NADP+ and NADPH are accompanied by binding and release of a proton. The enzyme was successfully reconstituted into liposomes by a cholate dilution procedure. The proteoliposomes catalysed cyclic NADPH-dependent reduction of AcPdAD+ by NADH only when they were tightly coupled. However, they catalysed cyclic NADP(+)-dependent reduction of AcPdAD+ by NADH only when they were uncoupled eg. by addition of carbonylcyanide-p-trifluoromethoxyphenyl hydrazone.(ABSTRACT TRUNCATED AT 250 WORDS)


Assuntos
Hidrogênio/metabolismo , Mitocôndrias Cardíacas/enzimologia , NADP Trans-Hidrogenases/metabolismo , Animais , Transporte Biológico , Catálise , Bovinos , Detergentes , Lipossomos , NADP Trans-Hidrogenases/antagonistas & inibidores , Oxirredução , Prótons , Solubilidade , Partículas Submitocôndricas/enzimologia , Temperatura
9.
Biochim Biophys Acta ; 1144(2): 225-8, 1993 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-8369341

RESUMO

Mitochondrial transhydrogenase has been reported previously to be inhibited by high, rather non-physiological concentrations (in the range of 2-20 mM) of divalent cations. We show that the enzyme could be activated by low (from about 1 microM to 1 mM) concentrations of Ca2+ and Mg2+, which are within physiological range. These results bring in line the effects observed with mitochondrial enzyme to the findings with bacterial transhydrogenases. The activation of transhydrogenase by divalent cations is interpreted as an increase in affinity of the NADP(H)-binding site of the enzyme-NAD(H) complex. Reported effects of the metal ions could be important for the enzyme function in vivo.


Assuntos
Cálcio/farmacologia , Magnésio/farmacologia , Mitocôndrias Cardíacas/enzimologia , NADP Trans-Hidrogenases/antagonistas & inibidores , Animais , Cátions Bivalentes , Bovinos , Ativação Enzimática , Modelos Químicos
10.
Eur J Biochem ; 211(3): 663-9, 1993 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-8436126

RESUMO

The effects of N,N'-dicyclohexylcarbodiimide [(cHxN)2C] on the proton-translocating enzyme, NAD(P) H(+)-transhydrogenase (H(+)-Thase), from two species of phototrophic bacteria have been investigated. The polypeptides of H(+)-Thase from Rhodobacter capsulatus are membrane-associated, requiring detergent to maintain solubility. The enzyme from Rhodospirillum rubrum, however, has a water soluble polypeptide (Ths) and a membrane-associated component (Thm) which, separately, have no activity but which can be fully reconstituted to give a functional complex. Two observations suggest that (cHxN)2C inhibited H(+)-Thase from both species by modification either close to or at the NADP(H)-binding site on the enzyme: (a) the presence of NADP+ or NADPH caused increased inhibition by (cHxN)2C and (b) after treatment of the purified enzyme from Rb. capsulatus with (cHxN)2C, the release of NADP+ became rate-limiting, as evidenced by a stimulated rate of NADPH-dependent reduction of acetylpyridine adenine dinucleotide by NADH. Experiments in which Ths and Thm from R. rubrum were separately treated with (cHxN)2C then reconstituted with the complementary, untreated component revealed that the NADP(H)-enhanced modification by (cHxN)2C was confined to Thm. In contrast to some experiments with mitochondrial H(+)-Thase [Wakabayashi, S. & Hatefi, Y. (1987) Biochem. Int. 15, 667-675], there was no protective effect of either NAD+ or NADH on the inhibition by (cHxN)2C of enzyme from photosynthetic bacteria. However, amino acid sequence analysis of proteolytic fragments of Ths revealed that the NAD(H)-protectable, (cHxN)2C-reactive glutamate residue in mitochondrial H(+)-Thase might be replaced by glutamine in R. rubrum.


Assuntos
Dicicloexilcarbodi-Imida/farmacologia , NADP Trans-Hidrogenases/antagonistas & inibidores , Rhodobacter capsulatus/enzimologia , Rhodospirillum rubrum/enzimologia , Sequência de Aminoácidos , Sítios de Ligação , Dados de Sequência Molecular , NAD/análogos & derivados , NAD/metabolismo , NAD/farmacologia , NADP/farmacologia , NADP Trans-Hidrogenases/química , Oxirredução , Fragmentos de Peptídeos/química
11.
Biochim Biophys Acta ; 1102(1): 19-29, 1992 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-1324729

RESUMO

Purified nicotinamide nucleotide transhydrogenase from beef heart was investigated with respect to labeling and subsequent sequence analysis of a nicotinamide nucleotide-binding site. A photo-activated azide derivative, 8-azidoadenosine 5'-monophosphate, was used as an active-site-directed photoaffinity label, which was shown to be specific for the NAD(H)-binding site in the dark. Light-activated incorporation of the label in transhydrogenase was accompanied by an inactivation, which approached 100% at the incorporation of about 1 mol label/mol transhydrogenase monomer. As expected from the assumed site-specificity of the label. NADH prevented both labeling and inactivation to some extent. However, NADPH also prevented labeling and inactivation marginally. The oxidized substrates NAD+ and NADP+ were inhibitory by themselves under these conditions, and the substrate analogs 5'-AMP and 2'-AMP were also poor protectors. The NAD(H)-site specificity of the azido compound was thus largely lost upon illumination and covalent modification. Radioactive labeling of transhydrogenase with 8-azido-[2-3H]-adenosine 5'-monophosphate followed by protease digestion, isolation of labeled peptides and amino-acid sequence analysis showed that Tyr 1006 in the sequence 1001-1027 close to the C-terminus was labeled. This sequence shows homologies with nucleotide-binding sequences in, e.g., F1-ATPase. On the basis of sequence homologies with other NAD(P)-dependent enzymes it is proposed that transhydrogenase contains 4 nucleotide-binding sites, of which 2 constitute the adenine nucleotide-binding domains of the catalytic sites for NAD(H) and NADP(H) close to the N- and C-terminals, respectively. Each of these domains has an additional vicinal nucleotide-binding sequence which may constitute a non-catalytic nucleotide-binding site or the nicotinamide nucleotide-binding domain of the catalytic site. The present results indicate that 8-azidoadenosine 5'-monophosphate is kinetically specific for the catalytic NAD(H)-binding site, but reacts covalently with Tyr 1006 of the putative non-catalytic site or nicotinamide nucleotide-binding domain formed by the 1001-1027 amino acid sequence of the catalytic NADP(H)-binding site. Interactions between the catalytic NAD(H) and NADP(H) binding sites, and the assumed non-catalytic sites, may be facilitated by a ligand-triggered formation of a narrow pocket, which normally allows an efficient hydride ion transfer between the natural substrates.


Assuntos
Miocárdio/enzimologia , NADP Trans-Hidrogenases/química , NADP/metabolismo , NAD/metabolismo , Marcadores de Afinidade , Sequência de Aminoácidos , Animais , Azidas/farmacologia , Sítios de Ligação , Bovinos , AMP Cíclico/análogos & derivados , AMP Cíclico/farmacologia , Dados de Sequência Molecular , NADP Trans-Hidrogenases/antagonistas & inibidores , NADP Trans-Hidrogenases/metabolismo , Homologia de Sequência do Ácido Nucleico , Tripsina
12.
J Biol Chem ; 266(26): 17020-5, 1991 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-1654321

RESUMO

The mitochondrial proton-translocating nicotinamide nucleotide transhydrogenase is embedded in the inner membrane as a homodimer of monomer Mr = 109,288. Its N-terminal 430 residues and C-terminal 200 residues protrude into the matrix, whereas its central 400 residues appear to intercalate into the inner membrane as 14 hydrophobic clusters of about 20 residues each (Yamaguchi, M., and Hatefi, Y. (1991) J. Biol. Chem. 266, 5728-5735). Treatment of mitoplasts (mitochondria denuded of outer membrane) with several proteolytic enzymes cleaves the transhydrogenase into a 72-kDa N-terminal and a 37-kDa C-terminal fragment. The cleavage site of proteinase K was determined to be Ala690-Ala691, which is located in a small loop of the transhydrogenase exposed on the cytosolic side of the inner membrane. This paper shows that the bisected transhydrogenase can be purified from proteinase K-treated mitoplasts with retention of greater than or equal to 85% transhydrogenase activity. The inactivation rate of the bisected enzyme by trypsin and N-ethylmaleimide was altered in the presence of NADP and NADPH, suggesting substrate-induced conformation changes similar to those reported previously for the intact transhydrogenase. Also, like the intact enzyme, proteoliposomes of the bisected transhydrogenase were capable of membrane potential formation and internal acidification coupled to NADPH----NAD transhydrogenation. The properties of the bisected transhydrogenase have been discussed in relation to those of the two-subunit Escherichia coli transhydrogenase, the bisected lac permease (via gene restriction), and the fragmented and reconstituted bacteriorhodopsin.


Assuntos
Mitocôndrias/enzimologia , NADP Trans-Hidrogenases/isolamento & purificação , Sequência de Aminoácidos , Animais , Bovinos , Eletroforese em Gel de Poliacrilamida , Endopeptidase K , Transferência de Energia , Cinética , Potenciais da Membrana , Dados de Sequência Molecular , NADP Trans-Hidrogenases/antagonistas & inibidores , NADP Trans-Hidrogenases/metabolismo , Conformação Proteica , Prótons , Serina Endopeptidases , Especificidade por Substrato , Tripsina
13.
Biokhimiia ; 55(2): 225-31, 1990 Feb.
Artigo em Russo | MEDLINE | ID: mdl-2160290

RESUMO

It was shown that the increase in the activities of transhydrogenase and NAD(+)-dependent isocitrate dehydrogenase after incubation of mitochondria with cAMP is due to the stimulating effect of cAMP on mitochondria, but not to the increased stability of mitochondria to the incubation procedure. Treatment of mitochondria with trypsin prevents the action of cAMP on the both enzymes. The integrity of the inner mitochondrial membrane is necessary for the manifestation of cAMP effect. Pretreatment of mitochondria with the local anesthetic, lidocaine, prevents the activation of NAD(P)(+)-transhydrogenase and NAD(+)-dependent isocitrate dehydrogenase during subsequent incubation of mitochondria with cAMP. It is concluded that the role of the inner mitochondrial membrane consists in the reception of the cAMP signal for the internal compartment of mitochondria, i.e. for mitoplasts. Peripheral protein(s) on the external side of the inner mitochondrial membrane seems to play a role in cAMP reception.


Assuntos
Membranas Intracelulares/enzimologia , Isocitrato Desidrogenase/metabolismo , Mitocôndrias Hepáticas/enzimologia , NADH NADPH Oxirredutases/metabolismo , NADP Trans-Hidrogenases/metabolismo , Animais , Cloromercurobenzoatos/farmacologia , AMP Cíclico/farmacologia , Ativação Enzimática , Feminino , Técnicas In Vitro , Isocitrato Desidrogenase/antagonistas & inibidores , Lidocaína/farmacologia , Masculino , NADP Trans-Hidrogenases/antagonistas & inibidores , Ratos , Ratos Endogâmicos , Tripsina/farmacologia
14.
Biochemistry ; 28(14): 6050-6, 1989 Jul 11.
Artigo em Inglês | MEDLINE | ID: mdl-2775749

RESUMO

The mitochondrial nicotinamide nucleotide transhydrogenase is a dimeric enzyme of monomer Mr 110,000. It is located in the inner mitochondrial membrane and catalyzes hydride ion transfer between NAD(H) and NADP(H) in a reaction that is coupled to proton translocation across the inner membrane. The amino acid sequence and the nucleotide binding sites of the enzyme have been determined [Yamaguchi, M., Hatefi, Y., Trach, K., & Hoch, J.A. (1988) J. Biol. Chem. 263, 2761-2767; Wakabayashi, S., & Hatefi, Y. (1987) Biochem. Int. 15, 915-924]. N-Ethylmaleimide, as well as other sulfhydryl group modifiers, inhibits the transhydrogenase. The presence of NADP in the incubation mixture suppressed the inhibition rate by N-ethylmaleimide, and the presence of NADPH greatly increased it. NAD and NADH had little or no effect. The NADPH effect was concentration dependent and saturable, with a half-maximal NADPH concentration effect close to the Km of the enzyme for NADPH. Study of the effect of pH on the N-ethylmaleimide inhibition rate showed that NADPH binding by the enzyme lowers the apparent pKa of the N-ethylmaleimide-sensitive group by 0.4 of a pH unit and NADP binding raises this pKa by 0.4 of a pH unit, thus providing a rationale for the effects of NADP and NADPH on the N-ethylmaleimide inhibition rate. With the use of N-[3H]ethylmaleimide, the modified sulfhydryl group involved in the NADP(H)-modulated inhibition of the transhydrogenase was identified as that belonging to Cys-893, which is located 113 residues upstream of the tyrosyl residue modified by [p-(fluorosulfonyl)benzoyl]-5'-adenosine at the putative NADP(H) binding site of the enzyme (see above references).(ABSTRACT TRUNCATED AT 250 WORDS)


Assuntos
NADH NADPH Oxirredutases/metabolismo , NADP Trans-Hidrogenases/metabolismo , Animais , Bovinos , Cisteína , Etilmaleimida/farmacologia , Concentração de Íons de Hidrogênio , Técnicas In Vitro , Cinética , Mitocôndrias/enzimologia , NADP , NADP Trans-Hidrogenases/antagonistas & inibidores , Especificidade por Substrato
15.
Biochemistry ; 27(20): 7665-71, 1988 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-3207696

RESUMO

Both purified and functionally reconstituted bovine heart mitochondrial transhydrogenase were treated with various sulfhydryl modification reagents in the presence of substrates. In all cases, NAD+ and NADH had no effect on the rate of inactivation. NADP+ protected transhydrogenase from inactivation by 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) in both systems, while NADPH slightly protected the reconstituted enzyme but stimulated inactivation in the purified enzyme. The rate of N-ethylmaleimide (NEM) inactivation was enhanced by NADPH in both systems. The copper-(o-phenanthroline)2 complex [Cu(OP)2] inhibited the purified enzyme, and this inhibition was substantially prevented by NADP+. Transhydrogenase was shown to undergo conformational changes upon binding of NADP+ or NADPH. Sulfhydryl quantitation with DTNB indicated the presence of two sulfhydryl groups exposed to the external medium in the native conformation of the soluble purified enzyme or after reconstitution into phosphatidylcholine liposomes. In the presence of NADP+, one sulfhydryl group was quantitated in the nondenatured soluble enzyme, while none was found in the reconstituted enzyme, suggesting that the reactive sulfhydryl groups were less accessible in the NADP+-enzyme complex. In the presence of NADPH, however, four sulfhydryl groups were found to be exposed to DTNB in both the soluble and reconstituted enzymes. NEM selectively reacted with only one sulfhydryl group of the purified enzyme in the absence of substrates, but the presence of NADPH stimulated the NEM-dependent inactivation of the enzyme and resulted in the modification of three additional sulfhydryl groups. The sulfhydryl group not modified by NEM in the absence of substrates is not sterically hindered in the native enzyme as it can still be quantitated by DTNB or modified by iodoacetamide.(ABSTRACT TRUNCATED AT 250 WORDS)


Assuntos
NADH NADPH Oxirredutases/metabolismo , NADP Trans-Hidrogenases/metabolismo , Animais , Sítios de Ligação , Bovinos , Reagentes de Ligações Cruzadas/farmacologia , Cisteína/metabolismo , Ácido Ditionitrobenzoico/farmacologia , Ditiotreitol/farmacologia , Etilmaleimida/farmacologia , Técnicas In Vitro , Mitocôndrias Cardíacas/enzimologia , NADP/metabolismo , NADP Trans-Hidrogenases/antagonistas & inibidores , Conformação Proteica , Reagentes de Sulfidrila/farmacologia
16.
Biochem J ; 252(3): 833-6, 1988 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-3138976

RESUMO

The biosynthesis of pyridine dinucleotide transhydrogenase has been studied in isolated rat hepatocytes and in a rabbit reticulocyte-lysate translation system supplemented with either intact isolated rat liver mitochondria or the soluble matrix fraction from isolated mitochondria. In intact hepatocytes, the transhydrogenase precursor was short-lived in the cytosol and was efficiently imported into the membranous fraction. When the cell-free translation mixture was incubated with intact mitochondria, the transhydrogenase precursor was processed to the mature form, to an extent that depended on the amount of added mitochondria. Incubation of the translation mixture with the soluble mitochondria matrix fraction converted the precursor to a mature-sized protein with 75% efficiency, this being blocked by various proteinase inhibitors such as EDTA, 1,10-phenanthroline and leupeptin.


Assuntos
Precursores Enzimáticos/metabolismo , Mitocôndrias Hepáticas/enzimologia , NADH NADPH Oxirredutases/metabolismo , NADP Trans-Hidrogenases/metabolismo , Animais , Transporte Biológico , Ácido Edético/farmacologia , Eletroforese em Gel de Poliacrilamida , Técnicas In Vitro , Leupeptinas/farmacologia , Fígado/enzimologia , Mitocôndrias Hepáticas/efeitos dos fármacos , NADP Trans-Hidrogenases/antagonistas & inibidores , Fenantrolinas/farmacologia , Ratos , Rodaminas/farmacologia
17.
Biochim Biophys Acta ; 953(3): 241-8, 1988 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-3128329

RESUMO

Modification of mitochondrial nicotinamide nucleotide transhydrogenase (NADPH: NAD+ oxidoreductase, EC 1.6.1.1) with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl), followed by measurement of the absorption or fluorescence of the transhydrogenase-NBD adducts, resulted in a biphasic labelling of approx. 4-6 sulfhydryls, presumably cysteine residues. Of these 1-2 (27%) were fast-reacting and 3-4 (73%) slow-reacting sulfhydryls. In the presence of substrates, e.g., NADPH, the labelling was monophasic and all sulfhydryls were fast-reacting, suggesting that the modified sulfhydryls are predominantly localized peripheral to the NAD(P)(H)-binding sites. The rates of modification allowed the calculation of the rate constants for each phase of the labelling. Both in the absence and in the presence of a substrate, e.g., NADPH, the extent of labelling essentially parallelled the inhibition of transhydrogenase activity. Attempts to reactivate transhydrogenase by reduction of labelled sulfhydryls were not successful. Photo-induced transfer of the NBD adduct in partially inhibited transhydrogenase, from the sulfhydryls to reactive NH2 groups of amino-acid residue(s), identified as lysine residue(s), was parallelled by an inhibition of the residual transhydrogenase activity. It is suggested that a lysine localized close to the fast-reacting NBD-Cl-reactive sulfhydryl groups is essential for activity.


Assuntos
4-Cloro-7-nitrobenzofurazano/farmacologia , Mitocôndrias Cardíacas/enzimologia , NADH NADPH Oxirredutases/antagonistas & inibidores , NADP Trans-Hidrogenases/antagonistas & inibidores , Oxidiazóis/farmacologia , Compostos de Sulfidrila , Animais , Bovinos , Fenômenos Químicos , Química , Cisteína , Cinética , Lisina , NAD/farmacologia , NADP/farmacologia , Fotoquímica , Espectrometria de Fluorescência , Espectrofotometria
18.
Biochim Biophys Acta ; 894(2): 239-51, 1987 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-2960379

RESUMO

The mechanism of coupling between mitochondrial ATPase (EC 3.6.1.3) and nicotinamide nucleotide transhydrogenase (EC 1.6.1.1) was studied in reconstituted liposomes containing both purified enzymes and compared with their behavior in submitochondrial particles. In order to investigate the mode of coupling between the transhydrogenase and the ATPase by the double-inhibitor and inhibitor-uncoupler methods, suitable inhibitors of transhydrogenase and ATPase were selected. Phenylarsine oxide and A3'-O-(3-(N-(4-azido-2-nitrophenyl)amino)propionyl)-NAD+ were used as transhydrogenase inhibitors, whereas of the various ATPase inhibitors tested aurovertin was found to be the most convenient. The inhibition of the ATP-driven transhydrogenase activity was proportional to the inhibition of both the ATPase and the transhydrogenase. Inhibitor-uncoupler titrations showed an increased sensitivity of the coupled reaction towards carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP)--an uncoupler that preferentially uncouples localized interactions, according to Herweijer et al. (Biochim. Biophys. Acta 849 (1986) 276-287)--when the primary pump was partially inhibited. However, when the secondary pump was partially inhibited the sensitivity towards FCCP remained unchanged. Similar results were obtained with submitochondrial particles. These results are in contrast to those obtained previously with the ATP-driven reverse electron flow. In addition, the amount of uncoupler required for uncoupling of the ATP-driven transhydrogenase was found to be similar to that required for the stimulation of the ATPase activity, both in reconstituted vesicles and in submitochondrial particles. Uncoupling of reversed electron flow to NAD+ required much less uncoupler. On the basis of these results, it is proposed that, in agreement with the chemiosmotic model, the interaction between ATPase and transhydrogenase in reconstituted vesicles as well as in submitochondrial particles occurs through the delta mu H+. In contrast, the energy transfer between ATPase and NADH-ubiquinone oxidoreductase appears to occur via a more direct interaction, according to the above-mentioned results by Herweijer et al.


Assuntos
Trifosfato de Adenosina/metabolismo , NADH NADPH Oxirredutases/análise , NADP Trans-Hidrogenases/análise , Partículas Submitocôndricas/enzimologia , Adenosina Trifosfatases/antagonistas & inibidores , Animais , Arsenicais/farmacologia , Carbonil Cianeto p-Trifluormetoxifenil Hidrazona/farmacologia , Bovinos , Transferência de Energia , NAD/metabolismo , NAD/farmacologia , NADP Trans-Hidrogenases/antagonistas & inibidores , Oxirredução
19.
Biochem Biophys Res Commun ; 142(2): 573-8, 1987 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-3028417

RESUMO

The effect of glutathione, glutathione disulfide and the dithiol reagent phenylarsine oxide on purified soluble as well as reconstituted mitochondrial nicotinamide nucleotide transhydrogenase from beef heart was investigated. Glutathione disulfide and phenylarsine oxide caused an inhibition of transhydrogenase, the extent of which was dependent on the presence of either of the transhydrogenase substrates. In the absence of NADPH glutathione protected partially against inactivation by glutathione disulfide and phenylarsine oxide. In the presence of NADPH glutathione also inhibited transhydrogenase. Reconstituted transhydrogenase vesicles behaved differently as compared to the soluble transhydrogenase and was partially uncoupled by GSSG. It is concluded that transhydrogenase contains a dithiol that is essential for catalysis as well as for proton translocation.


Assuntos
Mitocôndrias/enzimologia , NADH NADPH Oxirredutases/análise , NADP Trans-Hidrogenases/análise , Compostos de Sulfidrila/análise , Glutationa/farmacologia , NAD/farmacologia , NADP/farmacologia , NADP Trans-Hidrogenases/antagonistas & inibidores , Prótons
20.
Arch Biochem Biophys ; 243(1): 20-7, 1985 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-4062302

RESUMO

Mitochondrial energy-linked nicotinamide nucleotide transhydrogenase (TH; EC 1.6.1.1) was inactivated by treatment with pyridoxal phosphate, ethoxyformic anhydride (EFA) or dansyl chloride. NADP and NADPH, but not NAD and NADH, protected TH against inhibition by pyridoxal phosphate, and L-lysine reversed this inhibition. The results suggested modification of an essential lysyl residue by pyridoxal phosphate, possibly at the NADP(H) binding site of TH. EFA and dansyl chloride inhibited TH in a similar manner. The effect of pH on the rate of inhibition of TH by EFA and dansyl chloride was the same, and in both cases addition of NADP and particularly NADPH accelerated the rate of inhibition, while addition of NAD or NADH had no effect. Double inhibition studies, using in one experiment dithiothreitol-reversible inhibition by 5,5'-dithiobis(2-nitrobenzoic acid) to protect the thiol groups of TH, and in another experiment lysine-reversible inhibition by pyridoxal phosphate to protect the putative essential lysyl residues of the enzyme, followed in each case by further treatment of the protected TH with EFA or dansyl chloride, suggested that the inhibitions by EFA and dansyl chloride were independent of the inhibitions by 5,5'-dithiobis (2-nitrobenzoic acid) and pyridoxal phosphate. The inhibitors discussed above are interesting, because pyridoxal phosphate is the only reagent known which appears to modify an essential residue in the NADP(H), but not the NAD(H), binding site of TH, and EFA and dansyl chloride are the only inhibitors known which appear to react with essential residues outside the active site of TH. It is possible that EFA and dansyl chloride inhibitions involve modification of essential prototropic residues in the proton translocation domain of the enzyme.


Assuntos
Compostos de Dansil/farmacologia , Dietil Pirocarbonato/farmacologia , Formiatos/farmacologia , Mitocôndrias/enzimologia , NADH NADPH Oxirredutases/antagonistas & inibidores , NADP Trans-Hidrogenases/antagonistas & inibidores , Fosfato de Piridoxal/farmacologia , Animais , Dicicloexilcarbodi-Imida/farmacologia , Ácido Ditionitrobenzoico/farmacologia , Concentração de Íons de Hidrogênio , Cinética , NAD/metabolismo , NADP/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...