Visualization and body distribution of [¹³¹I]-herceptin in nude mice with BT-474 breast carcinoma.

The study aimed to investigate the bio-distribution and radio-immuno-imaging features of [(131)I]-herceptin in nude mice with BT-474 breast carcinoma. [(131)I]-Herceptin was administrated by tail intravenous injection to the nude mice with BT-474 breast carcinoma. Radiocounting was performed at 4, 12, 24, 48, and 96 h after administration. The activity ratio in the tumor tissue and non-tumor tissue (T/NT) and the radiocounting percentage per gram tissue to the injected dose (%ID/g) were calculated. The nude mice with BT-474 breast carcinoma were also visualized continuously by single photon emission computed tomography at 2, 4, 8, 12, 24, 48, and 96 h after the injection of [(131)I]-herceptin. Nude mice with MDA-MB-231 used as the control group were subjected to the same analyses. Clear tumor images were obtained after the injection of [(131)I]-herceptin in nude mice with BT-474 breast carcinoma. The images were the clearest at 24 h after the injection and remained clear even at 96 h. The T/NT ratio and %ID/g in the tumor tissues of nude mice with BT-474 were both significantly higher than those of the control group (P < 0.01). [(131)I]-Herceptin displays tumors clearly in the nude mice with BT-474 and accumulates well in the tumor tissues.

99mTc direct labeling of anti-CEA monoclonal antibodies: quality control and preclinical studies.

The anti-carcinoembryonic B2C114 monoclonal antibody was radiolabeled with 99mTc by a direct method and quality control tested in vitro by instant thin layer chromatography, gel column scanning and cellulose acetate electrophoresis and assessed in vivo for radioimmunodetection on a murine spontaneous mammary carcinoma. The optimal results of percent 99mTc bound to protein were obtained at a dithiothreitol:antibody molar ratio ranging from 800:1 to 1000:1 and at a methylene diphosphonate:stannous fluoride weight ratio of 4.3:1. Although cysteine removed up to 18% of the label during the first 4 h, the stability of the tracer appeared to be excellent in human serum at 37 degrees C and when challenged with DTPA. 99mTc-labeled B2C114 demonstrated good and specific in vivo tumor targeting.

Um novo dispositivo no auxilio da punçao aspirativa por agulha fina nos tumores solidos de origem mamaria: estudo piloto em ratos / A new device to help fine needle aspiration cytology in solid mammary tumors: preliminary study in rats

A punçao aspirativa por agulha fina (PAAF) e considerada uma das bases no diagnostico de tumores solidos da mama. Apesar de simples, o metodo apresenta certas dificuldades tecnicas.Com o objetivo de facilitar o exame, foi idealizado um dispositivo em que uma mola de aço e montada em uma seringa de 5ml, gerando pressao negativa constante e permitindo que o examinador se preocupe exclusivamente com o direcionamento da agulha. Sao apresentados os resultados preliminares da PAAF com o dispositivo em 22 tumores de origem mamaria inoculados em ratos (11 Spl e 11 carcinossarcomas de Walker-256). Tais tumores apresentam consistencias diferentes, elasticas e petreas respectivamente. Assim foi possivel aspirar material em quantidade satisfatoria para a leitura citologica, consequentemente confirmando o diagnostico de malignidade em todos os casos de ambos os grupos. Tais numeros abonam a realizaçao de estudos futuros, comparando o uso do dispositivo apresentado com o procedimento classico em mulheres