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1.
Dis Markers ; 2021: 5546858, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34234876

RESUMO

OBJECTIVES: To investigate the relationship between polymorphisms of calcitonin-related peptide gene II (beta-calcitonin gene-related peptide (ßCGRP), CALCB) and serum CGRP levels in salivary adenoid cystic carcinoma. MATERIALS AND METHODS: Using the polymerase chain reaction (PCR) technique, the full-length amplification and genotype analysis of CALCB genes were performed in 39 patients with adenoid cystic carcinoma of salivary gland and 158 normal controls. The gene frequencies of major genotype of CALCB in adenoid cystic carcinoma of salivary gland and normal control group were analyzed. Enzyme-linked immunosorbent assay (ELISA) was used to evaluate serum calcitonin gene-related peptide (CGRP) and its concentration of alpha and beta subtypes. RESULTS: Univariate logistic regression analysis showed that the CALCB rs2839222 T/T genotype was closely related to the occurrence of salivary adenoid cystic carcinoma, with a correlation coefficient of 3.89. CONCLUSIONS: The serum CGRP concentration in the salivary adenoid cystic carcinoma group was 1.56 times that of the normal control group. The αCGRP subtype was significant, which was 3.02 times that of the normal control. The polymorphism of ßCGRP gene is associated with genetic susceptibility to salivary adenoid cystic carcinoma, and serum CGRP and ßCGRP can be used as novel markers of salivary adenoid cystic carcinoma.


Assuntos
Biomarcadores Tumorais/genética , Peptídeo Relacionado com Gene de Calcitonina/genética , Carcinoma Adenoide Cístico/genética , Genótipo , Polimorfismo Genético , Neoplasias das Glândulas Salivares/genética , Adulto , Idoso , Biomarcadores Tumorais/sangue , Peptídeo Relacionado com Gene de Calcitonina/sangue , Carcinoma Adenoide Cístico/sangue , Carcinoma Adenoide Cístico/diagnóstico , Estudos de Casos e Controles , Ensaio de Imunoadsorção Enzimática , Feminino , Predisposição Genética para Doença , Humanos , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Neoplasias das Glândulas Salivares/sangue , Neoplasias das Glândulas Salivares/diagnóstico
2.
Indian J Pathol Microbiol ; 64(1): 22-27, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33433405

RESUMO

BACKGROUND: Salivary gland tumors bear uncanny characteristics of being different based on their morphological aspects rather than the presence of clear demarcation. This ambiguity in the spectrum from benign to malignant salivary gland neoplasms while categorizing the neoplasm is having inherent pitfalls. The present study was, therefore, designed to characterize benign and malignant salivary gland tumors based on their proliferative indices. MATERIALS AND METHOD: Study samples comprised of 97 cases of histopathologically confirmed benign and malignant salivary gland tumors. The cases were immunohistochemically assessed for MCM3 and Ki-67 expressions and the molecular characterization was performed based on the findings. RESULTS: The majority of benign and malignant salivary gland tumors were from the parotid gland, (51.2%) and (42.4%), respectively. Overall mean labeling index of MCM3 was higher i.e., (5.60 ± 3.99) in comparison to Ki-67 i.e., (2.82 ± 3.14) with P = 0.05 using paired t-test. Besides, malignant salivary gland neoplasms represented a higher mean score of MCM3 and Ki-67 than benign neoplasms. CONCLUSION: The requirement of a novel marker has led to the use of MCM3 which has a characteristic role in the entire spectrum of the cell cycle. The present study highlighted the extrapolation of MCM3 over Ki-67 for diagnosis and for true characterization of biologic behavior of salivary gland pathologies which may, in turn, influence the treatment modality employed for such lesions.


Assuntos
Antígeno Ki-67/genética , Componente 3 do Complexo de Manutenção de Minicromossomo/genética , Neoplasias das Glândulas Salivares/diagnóstico , Biomarcadores Tumorais/análise , Biomarcadores Tumorais/genética , Feminino , Humanos , Antígeno Ki-67/análise , Masculino , Pessoa de Meia-Idade , Componente 3 do Complexo de Manutenção de Minicromossomo/análise , Inclusão em Parafina , Neoplasias das Glândulas Salivares/sangue , Neoplasias das Glândulas Salivares/secundário , Glândulas Salivares/patologia
3.
Asian Pac J Cancer Prev ; 20(2): 545-548, 2019 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-30803218

RESUMO

Background: Variation in serum levels of trace elements including zinc, copper and ferritin has been reported in cancer patients. The aim of this study was to evaluate these trace elements in the patients' sera with benign and malignant salivary gland tumors (SGTs) and compare them with normal individuals. Methods: In this cross-sectional study, 60 patients with SGTs including 16 pleomorphic adenoma and 44 malignant SGTs, as well as 28 healthy controls, were enrolled. Serum levels of zinc, copper and ferritin were determined by atomic absorption and ELISA methods. Data were analyzed using one way ANOVA, Chi-square, Kruskal-Wallis and Mann- Whitney tests. Results: The mean concentration of zinc, copper, ferritin was1.5± 2 ppm, 1.2± 0.5 ppm, and 96.7± 65.7 ng/ml in PA, 1.5± 1.4,1.3± 0.4, and 111.2± 112 in malignant SGTs, and1.1±0.3, 1.2± 0.23 and 124±135.8 in normal control groups. There was no statistically significant difference between the patients and control groups, and between benign and malignant SGTs (P>0.05). Conclusion: The serum levels of trace elements in SGTs were not different from normal individuals. The results might have been affected by some interventional factors. Therefore, designing cohort complementary studies might result in obtaining more accurate data.


Assuntos
Cobre/sangue , Ferritinas/sangue , Neoplasias das Glândulas Salivares/sangue , Neoplasias das Glândulas Salivares/patologia , Oligoelementos/sangue , Zinco/sangue , Adulto , Estudos de Casos e Controles , Estudos Transversais , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico
4.
Cancer Biomark ; 23(1): 61-65, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29991126

RESUMO

BACKGROUND: Interleukin-33 (IL-33) has been recently discovered as an influential factor in the process of tumor immunity, and is presented in cancer pathogenesis. OBJECTIVE: This study aimed to determine the serum levels of IL-33 in patients with benign and malignant Salivary gland tumors (SGTs). METHODS: This descriptive cross-sectional study was performed on 47 samples of malignant SGTs including 18 mucoepidermoid carcinoma (MEC), 8 adenoid cystic carcinoma (ADCC), 21 malignant mixed tumor (MMT), and 14 benign pleomorphic adenoma (PA). A control group was considered consisting of 28 healthy subjects. The serum level of IL-33 was measured by using sandwich ELISA method. The data were statistically analyzed through Kruskal-Wallis and Mann-Whitney tests. RESULTS: The median concentration of IL-33 was 6.91 in malignant, 5.14 in benign, and 5.01 in healthy cases, with a statistically significant difference (P= 0.001). The median serum levels of IL-33 increased significantly in ADCC (7.15), MEC (7.03), and MMT (6.91) compared with the control group (5.01) (P< 0.05). The mean rank of MEC was significantly higher than PA (P= 0.01). IL-33 concentration was positively and significantly correlated with the tumor stage (P= 0.02) and tumor size (P= 0.03). CONCLUSIONS: IL-33 could be suggested as a novel biomarker to distinguish different types of SGTs.


Assuntos
Biomarcadores Tumorais/sangue , Interleucina-33/sangue , Neoplasias/sangue , Neoplasias das Glândulas Salivares/sangue , Adenoma Pleomorfo/sangue , Adenoma Pleomorfo/patologia , Adulto , Idoso , Carcinoma Adenoide Cístico/sangue , Carcinoma Adenoide Cístico/patologia , Carcinoma Mucoepidermoide/sangue , Carcinoma Mucoepidermoide/patologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias/classificação , Neoplasias/patologia , Neoplasias das Glândulas Salivares/classificação , Neoplasias das Glândulas Salivares/patologia
5.
Cytokine ; 106: 40-44, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29549722

RESUMO

OBJECTIVES: The aim of this study was to evaluate serum levels of adiponectin, leptin, visfatin and IL-6 in patients with pleomorphic adenoma, Warthin's tumor and acinic cell carcinoma of the parotid gland. MATERIALS AND METHODS: Venous blood samples were collected from 30 patients with pleomorphic adenoma, 21 patients with Warthin's tumor and 8 patients with acinic cell carcinoma. Serum adiponectin, leptin, visfatin, IL-6 and CRP concentrations were determined. RESULTS: Our results revealed significantly lower adiponectin serum levels in patients with malignant tumors compared to benign tumor individuals. Moreover, in benign cases the level was significantly higher compared to controls. Furthermore, serum leptin concentrations of benign tumor patients were higher compared to controls. Those differences, however, were observed only in males. The serum visfatin level was elevated in all tumor subjects compared to healthy individuals, whereas the serum IL-6 concentration was similar. CONCLUSIONS: We anticipate that adiponectin may play a potential protective role in salivary gland tumors. Also leptin and visfatin seem to play an important role in salivary gland tumor pathology, although in males and females leptin may act or be regulated in a different manner. The influence of visfatin on salivary gland tumors is probably independent of IL-6 production.


Assuntos
Adipocinas/sangue , Glândula Parótida/patologia , Neoplasias das Glândulas Salivares/sangue , Neoplasias das Glândulas Salivares/patologia , Adiponectina/sangue , Feminino , Humanos , Interleucina-6/sangue , Leptina/sangue , Masculino , Pessoa de Meia-Idade , Nicotinamida Fosforribosiltransferase/sangue
6.
Asian Pac J Cancer Prev ; 19(3): 689-692, 2018 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-29580041

RESUMO

Objective: To identify serum levels of galectin-3 in salivary gland cancer and healthy populations; a prospective analysis was performed on serum specimens from 105 patients with salivary gland cancer and 56 healthy persons. Methods: Enzyme-linked immunosorbent assay (ELISA) was used to measure levels of galectin-3 (GAL-3). Serum levels were compared between patients with salivary gland tumors and healthy control. A total of 105 patients were enrolled in the study (55 men, 50 women). Result: Mean age was 45.5 years. Thirty-nine patients with malignant and 66 cases with benign tumors were compared with 56 healthy participants with a mean age of 51.7. No statistically significant differences were observed when comparing GAL-3 serum levels between malignant and benign salivary gland tumor patients, but a statistically significant difference was found between case and control patients with p-values of 0.02. Serum levels of galectin-3 protein were elevated in patients with salivary gland cancer compared with the healthy population. Conclusion: The difference between benign and malignant tumor patients was significant, but revealed no clinic pathological characteristics in malignant tumors. To the best of the authors' knowledge, this is the first time a study suggests that GAL-3 serum levels could help clinicians screen for salivary gland cancer.


Assuntos
Biomarcadores Tumorais/sangue , Galectina 3/sangue , Neoplasias das Glândulas Salivares/sangue , Neoplasias das Glândulas Salivares/diagnóstico , Proteínas Sanguíneas , Estudos de Casos e Controles , Feminino , Seguimentos , Galectinas , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico , Estudos Prospectivos
7.
Oncotarget ; 8(1): 1083-1091, 2017 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-27894101

RESUMO

The prognostic role of modified Glasgow Prognostic Score (mGPS), neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) in patients with salivary duct carcinoma (SDC) remains unclear. We conducted a multi-institutional retrospective cohort study of 140 SDC patients. The survival impact of these hematological markers was evaluated using multivariate proportional hazard models.High mGPS (≥1) was significantly associated with worse survival (3-year overall survival (OS): 16.7% vs 66.1%, p-value=0.003; 3-year progression-free survival (PFS): 0.0% vs 27.9%, p-value<0.001). Additionally, high C-reactive protein (CRP) (≥0.39 mg/dl) was significantly associated with worse survival (3-year OS: 32.1% vs 68.2%, p-value=0.001; 3-year PFS: 7.1% vs 31.1%, p-value<0.001). These associations were consistent with multivariate analysis adjusted for established prognostic factors. Although we also found significant association of high NLR (≥2.5) with OS (HR 1.80; 95% confidence interval, 1.05-3.08) in multivariate analysis, this association were inconsistent with the results of PFS. In addition, we found no significant associations of PLR with survival. In conclusion, we found that mGPS, CRP and NLR were identified as prognostic factors associated with survival in SDC patients.


Assuntos
Biomarcadores/sangue , Mediadores da Inflamação/sangue , Ductos Salivares/patologia , Neoplasias das Glândulas Salivares/sangue , Neoplasias das Glândulas Salivares/mortalidade , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Japão , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Prognóstico , Neoplasias das Glândulas Salivares/diagnóstico , Neoplasias das Glândulas Salivares/terapia
8.
Asian Pac J Cancer Prev ; 17(7): 3601-4, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27510015

RESUMO

BACKGROUND: The monocyte chemoattractant protein1 (MCP1/CCL2) is a potent chemoattractant for natural killer cells, monocytes, and memory T lymphocytes. However, any role in the genesis of salivary gland tumors (SGT) is unknown. To assess the diagnostic relevance of chemokines in SGT, MCP1 levels in the serum of patients were investigated in association with tumor progression and clinical aggressiveness. MATERIALS AND METHODS: Using an ELISA kit, we assessed and compared the circulating levels of MCP1 in blood serum of 70 SGT patients with 44 healthy control samples. RESULTS: The results of this study showed that the concentration of MCP1 was significantly lower in patients with benign (463.8±158.5pg/ml, P=0.033) and malignant (454.8±190.4pg/ ml, P=0.007) SGT than in healthy subjects (645.7±338.9). No significant difference in mean serum levels of MCP1 was observed between the benign and malignant group (p=0.9). While MCP1 levels were lower in patients with an advanced clinical stage, advanced tumor size, higher tumor grade, or lymph node involvement, but the mean MCP1 level between groups showed no statistically significant difference (p>0.05). CONCLUSIONS: MCP1 levels in the serum of patients with SGT were decreased, indicating that this might a good marker for discriminating patients with SGT from healthy people. However, no clearcut relationship was detected between MCP1 levels and clinicopathologic factors, and MCP1 is not a good marker for evaluating tumor dissemination.


Assuntos
Quimiocina CCL2/sangue , Neoplasias das Glândulas Salivares/sangue , Adulto , Biomarcadores Tumorais/sangue , Estudos de Casos e Controles , Progressão da Doença , Feminino , Humanos , Linfonodos/patologia , Masculino , Neoplasias das Glândulas Salivares/patologia
9.
Otolaryngol Head Neck Surg ; 155(6): 988-996, 2016 12.
Artigo em Inglês | MEDLINE | ID: mdl-27436419

RESUMO

OBJECTIVE: To assess pretreatment levels in the counts and percentages of leukocytes and the neutrophil-lymphocyte ratio (NLR) in benign and malignant salivary gland tumors (SGTs) while investigating whether NLR is an inflammatory marker for distinguishing low- from high-grade parotid gland tumors. STUDY DESIGN: Case series with chart review. SETTING: Tertiary referral center. SUBJECTS AND METHODS: This study was performed on 182 patients with SGTs (age range: 16-87 years; 93 male and 89 female) who were treated between January 2010 and May 2015. Pretreatment counts and percentages of leukocytes and NLR were measured preoperatively in benign and malignant tumors. RESULTS: Mean neutrophil percentage (63.50% ± 12.11% vs 58.76% ± 8.20%, P = .008) and NLR (3.29 ± 3.13 vs 2.13 ± 1.26, P = .008) were significantly higher in patients with malignant SGTs than in patients with benign SGTs. Mean lymphocyte count (2.42 ± 0.72 103/mm3 vs 1.97 ± 0.87 103/mm3, P < .001) and percentage (30.67% ± 7.68% vs 26.86% ± 10.15%, P = .011) were lower in patients with malignant SGTs than in patients with benign SGTs. Mean lymphocyte percentage and NLR were significantly different between low- and high-grade malignant parotid gland tumors (P = .026 and P = .030, respectively). CONCLUSION: Elevated NLR could be an inflammatory marker to distinguish low- from high-grade malignant parotid gland tumors.


Assuntos
Linfócitos/citologia , Neutrófilos/citologia , Cuidados Pré-Operatórios , Neoplasias das Glândulas Salivares/sangue , Neoplasias das Glândulas Salivares/patologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Contagem de Linfócitos , Masculino , Sistemas Computadorizados de Registros Médicos , Pessoa de Meia-Idade , Neoplasias Parotídeas/sangue , Neoplasias Parotídeas/patologia , Valor Preditivo dos Testes , Prognóstico , Estudos Retrospectivos , Neoplasias das Glândulas Salivares/imunologia , Sensibilidade e Especificidade , Centros de Atenção Terciária
10.
Oral Dis ; 22(6): 566-72, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27120402

RESUMO

OBJECTIVE: The objective of this study was to explore the prevalence of T helper type 1 (Th1; CD4(+) IFN-γ (+) ) and Th2 (CD4(+) IL-4(+) ) cells, as well as cytotoxic T cell type 1 (Tc1; CD8(+) IFN-γ(+) ) and Tc2 cells (CD8(+) IL-4(+) ) in peripheral blood of the patients with salivary gland tumors (SGTs). SUBJECTS AND METHODS: Thirty new patients with SGTs and 15 healthy controls were recruited. After intracellular cytokine staining, data acquisition and analysis were performed by flow cytometry. RESULTS: The mean percentages of Th1 and Tc1 cells, as well as the ratios of Th1/Th2 and Tc1/Tc2, were observed to be significantly lower in patients with malignant SGTs in comparison with controls. Furthermore, the geometric mean fluorescent intensity (geometric MFI, representing the cytokine expression intensity) for IL-4 production by Th2 and Tc2 lymphocytes was significantly higher in patients with malignant tumors than controls. Positive correlations were observed between the mean percentage of Tc2 cells with Th2 cells, and with the tumor size in patients with benign and malignant tumors, respectively. CONCLUSION: The findings suggest that the imbalance of Th1/Th2 and Tc1/Tc2 ratios, as well as the increase in the expression of IL-4 by Th2 and Tc2 lymphocytes, may contribute to the pathogenesis of SGTs, especially in malignant cases.


Assuntos
Linfócitos T CD8-Positivos , Neoplasias das Glândulas Salivares/sangue , Linfócitos T Citotóxicos , Células Th1 , Células Th2 , Contagem de Células Sanguíneas , Humanos , Interleucina-4/biossíntese
11.
Iran J Immunol ; 13(1): 9-15, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27026042

RESUMO

BACKGROUND: Salivary gland tumors are among malignancies that have high recurrence rate. Immune responses in salivary gland tumors have not been well elucidated. T helper type 1 (Th1) and Th2 cytokines have been reported to play a role in the outcome of head and neck cancers. OBJECTIVE: To evaluate the serum levels of interferon gamma (IFN-γ), as the hallmark of Th1 cytokines, and interleukin-4 (IL-4), as the hallmark of Th2 cytokines, in patients with benign and malignant salivary gland tumors in comparison with healthy controls. METHODS: Fifty patients with benign and 14 patients with malignant salivary gland tumors, as well as 23 healthy individuals were recruited. Serum levels of IFN-γ and IL-4 were measured using ELISA method. Nonparametric tests were used for data analysis. RESULTS: Serum levels of IFN-γ and IL-4 were found not to be significantly different in patients compared to the control group (0.68±0.29 vs. 1.03±0.57pg/ml, p=0.58 for IFN-γ, 4.57±1.57vs. 4.41±1.31pg/ml, p=0.28 for IL-4). IFN-γ and IL-4 serum levels were also not significantly different between patients with benign and malignant salivary gland tumors (p=0.54 and p=0.86, respectively). CONCLUSION: The systemic levels of IL-4 and IFN-γ seem not to be associated with salivary gland tumor in our study. Investigation of other cytokines produced by Th1 and Th2 cells are warranted.


Assuntos
Citocinas/metabolismo , Neoplasias das Glândulas Salivares/diagnóstico , Neoplasias das Glândulas Salivares/metabolismo , Células Th1/metabolismo , Células Th2/metabolismo , Adulto , Idoso , Idoso de 80 Anos ou mais , Estudos de Casos e Controles , Citocinas/sangue , Humanos , Pessoa de Meia-Idade , Gradação de Tumores , Metástase Neoplásica , Estadiamento de Neoplasias , Neoplasias das Glândulas Salivares/sangue , Neoplasias das Glândulas Salivares/imunologia , Células Th1/imunologia , Células Th2/imunologia , Adulto Jovem
12.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 51(1): 53-7, 2016 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-26792189

RESUMO

IgG4-related disease is a novel clinical entity which can affect single or multiple organs. IgG4-related sialadenitis is referred to the salivary gland involvement of IgG4-related disease, with or without other organ involvement. IgG4-related sialadenitis is characterized by painless swelling or enlargement of salivary glands, high serum IgG4 level, abundant IgG4+ plasma cells infiltration with fibrosis histologically, and good response to glucocorticoids. With review of related articles, highlight and provide an overview of the most recent and focused findings and concepts of this disease, including the most significant pathogenic process based on kinds of immunocytes, cytokines, as well as participation of epithelial-mesenchymal transition, the clinical value of elevated serum IgG4 concentration and pathological role of infiltrated IgG4+ plasma cells, the potential relationship with salivary gland malignant tumor, the applying and usefulness of positron emission tomography-CT, the diagnostic utility of lip biopsy, treatment, prognosis, and also future perspectives.


Assuntos
Imunoglobulina G/sangue , Sialadenite/sangue , Biópsia , Glucocorticoides/uso terapêutico , Humanos , Paraproteinemias , Tomografia por Emissão de Pósitrons , Prognóstico , Neoplasias das Glândulas Salivares/sangue , Glândulas Salivares/imunologia , Sialadenite/tratamento farmacológico , Sialadenite/imunologia
13.
Cancer Radiother ; 19(2): 106-10, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25457791

RESUMO

Carcinoembryonic antigen is a tumour marker commonly increased in gastrointestinal and pulmonary cancers. We report a case of a 46-year-old man with a mucoepidermoid carcinoma of the base of tongue with an elevated and traceable serum carcinoembryonic antigen level. This antigen proved to be a valuable marker in the treatment follow-up. When a raised carcinoembryonic antigen level is found, salivary gland malignancies should be taken into the differential diagnosis and clinical examination of the head and neck region should not be overlooked.


Assuntos
Biomarcadores Tumorais/sangue , Antígeno Carcinoembrionário/sangue , Carcinoma Mucoepidermoide/sangue , Neoplasias das Glândulas Salivares/sangue , Neoplasias da Língua/sangue , Transtornos da Articulação/etiologia , Esôfago de Barrett/diagnóstico , Carcinoma Mucoepidermoide/diagnóstico por imagem , Carcinoma Mucoepidermoide/radioterapia , Carcinoma Mucoepidermoide/cirurgia , Terapia Combinada , Transtornos de Deglutição/etiologia , Diagnóstico Tardio , Seguimentos , Refluxo Gastroesofágico/diagnóstico , Refluxo Gastroesofágico/tratamento farmacológico , Humanos , Masculino , Pessoa de Meia-Idade , Especificidade de Órgãos , Inibidores da Bomba de Prótons/uso terapêutico , Radioterapia Adjuvante , Indução de Remissão , Neoplasias das Glândulas Salivares/diagnóstico por imagem , Neoplasias das Glândulas Salivares/radioterapia , Neoplasias das Glândulas Salivares/cirurgia , Glândulas Salivares Menores/patologia , Glândulas Salivares Menores/cirurgia , Tomografia Computadorizada por Raios X , Neoplasias da Língua/diagnóstico por imagem , Neoplasias da Língua/radioterapia , Neoplasias da Língua/cirurgia
14.
Zhonghua Bing Li Xue Za Zhi ; 43(9): 618-22, 2014 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-25471504

RESUMO

OBJECTIVE: To evaluate the clinical and pathological features of IgG4-related disease (IgG4RD). METHODS: The clinical data, laboratory profiles, radiological, pathological and therapeutic features of eight cases of IgG4RD were analyzed. This cohort included two cases of common bile duct and partial hepatectomy specimens, two of submandibular gland excision specimens, one from lung biopsy specimen, one from open lung biopsy specimen, one from renal biopsy specimen, and one from renal excision specimen. In all cases, adequate lesion tissues were obtained. They were paraffin embedded, HE stained, and additional special stains and immunohistochemistry performed (MaxVision method). RESULTS: This series consisted of five males and three females, with a mean age of onset of 60 years. Five cases were suspected to be malignant pre-operatively, including two cases suspected of common bile duct carcinoma, two suspected of salivary gland tumor, and one suspected of renal pelvic carcinoma. Elevated serum levels of IgG4 and IgE were detected in five cases and eosinophilia in four cases. Multi-organ involvement was noted in four cases. The major histopathological features associated with IgG4-RD were: dense lymphoplasmacytic infiltrate, with lymphoid follicle formation. Extensive eosinophilic infiltrate (> 10/HPF) was seen in four cases; fibrosis that was arranged at least focally in a storiform pattern was also noted. The numbers of IgG4 positive plasma cells were > 20-50/HPF, while the IgG4 to IgG ratio was more than 40%. Obliterative phlebitis was present in four cases. Other pathological changes such as necrotizing vasculitis or lymphoma were not found. Five patients responded well to glucocorticoids. CONCLUSIONS: IgG4RD has relatively specific histopathological features; accurate evaluation of the absolute and relative number of IgG4 positive plasma cells in lesional tissue, combining with clinical examination and exclusion of other causes of elevated IgG4, allows the diagnosis of IgG4RD. IgG4RD has complicated clinical manifestation, and glucocorticoids therapy is efficacious.


Assuntos
Neoplasias do Ducto Colédoco/patologia , Imunoglobulina G/sangue , Neoplasias Renais/patologia , Neoplasias das Glândulas Salivares/patologia , Biópsia , Neoplasias do Ducto Colédoco/sangue , Feminino , Fibrose/patologia , Humanos , Imunoglobulina E/sangue , Imuno-Histoquímica , Neoplasias Renais/sangue , Pelve Renal/patologia , Pulmão/patologia , Masculino , Pessoa de Meia-Idade , Plasmócitos , Neoplasias das Glândulas Salivares/sangue
15.
Ann Hematol ; 93(8): 1287-95, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24633660

RESUMO

We have performed a retrospective analysis of all patients with extragastric mucosa-associated lymphoid tissue (MALT) lymphoma treated at our institution to compare the efficacy of first-line therapeutic modalities including surgery, radiation, systemic therapy, and antibiotics. One hundred eighty-five patients with extragastric MALT lymphoma with a median age of 63 (interquartile range (IQR) 50-74) years and a median follow-up time of 49 (IQR 18-103) months were retrospectively analyzed. Time to progression and time to next therapy were used as surrogate endpoints for efficacy. Patients having either surgery (100 %), chemo/immunotherapy (85.5 %), or radiation (80 %) had significantly (p = 0.01) higher response rates than patients treated with antibiotics (33.3 %). Patients who were irradiated had significantly more progressive disease, but also the longest follow-up time. Stage, elevated LDH, anemia, elevated beta-2 microglobulin, plasmacytic differentiation, monoclonal gammopathy, or autoimmune disease did not influence the rate of disease progression nor did complete remission or partial remission from initial therapy influence time to and rate of progression. There was no significant difference in the median time to progression (p = 0.141), but the estimated time to progression (p = 0.023) as well as the estimated time to next therapy (p = 0.021) was significantly different among the various cohorts favoring surgery, chemo/immunotherapy, and radiation. Our results suggest extragastric MALT lymphoma as a potential systemic disease irrespective of initial stage. Radiation, surgery, and chemo/immunotherapy seem to be equally effective in achieving remissions and prolonged progression free survivals, but a curative potential is questionable. Localized MALT lymphomas affecting the thyroid gland or the lungs have excellent long-term progression-free survivals with surgical treatment only.


Assuntos
Linfoma de Zona Marginal Tipo Células B/terapia , Idoso , Idoso de 80 Anos ou mais , Antibacterianos/uso terapêutico , Antineoplásicos/uso terapêutico , Áustria/epidemiologia , Neoplasias Colorretais/sangue , Neoplasias Colorretais/mortalidade , Neoplasias Colorretais/terapia , Terapia Combinada , Progressão da Doença , Intervalo Livre de Doença , Neoplasias Oculares/sangue , Neoplasias Oculares/mortalidade , Neoplasias Oculares/radioterapia , Neoplasias Oculares/cirurgia , Neoplasias Oculares/terapia , Humanos , Imunoterapia , Estimativa de Kaplan-Meier , Neoplasias Pulmonares/sangue , Neoplasias Pulmonares/mortalidade , Neoplasias Pulmonares/radioterapia , Neoplasias Pulmonares/cirurgia , Neoplasias Pulmonares/terapia , Linfoma de Zona Marginal Tipo Células B/sangue , Linfoma de Zona Marginal Tipo Células B/mortalidade , Linfoma de Zona Marginal Tipo Células B/radioterapia , Linfoma de Zona Marginal Tipo Células B/cirurgia , Pessoa de Meia-Idade , Especificidade de Órgãos , Estudos Retrospectivos , Neoplasias das Glândulas Salivares/sangue , Neoplasias das Glândulas Salivares/mortalidade , Neoplasias das Glândulas Salivares/radioterapia , Neoplasias das Glândulas Salivares/cirurgia , Neoplasias das Glândulas Salivares/terapia , Neoplasias da Glândula Tireoide/sangue , Neoplasias da Glândula Tireoide/mortalidade , Neoplasias da Glândula Tireoide/cirurgia , Neoplasias da Glândula Tireoide/terapia , Resultado do Tratamento
16.
BMJ Case Rep ; 20132013 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-23661663

RESUMO

Polymorphous low-grade adenocarcinoma (PLGA) originating mostly in the minor salivary glands of the posterior hard and soft palate is characterised by its indolent growth and a slower rate of metastasis. Seldom does the PLGA present an aggressive behaviour and demonstrate distant metastasis, as in the present case where a 73-year-old female patient with a swelling in the maxillary alveolus was diagnosed as PLGA exhibiting high-grade transformation, subsequently metastasizing to the abdomen and lungs. The importance of immunomarkers, c-kit and ki-67 in deciphering the clinical behaviour of this PLGA is highlighted. Distant metastasis to the abdomen has not yet been reported; hence, this case of PLGA emphasises the importance of immunohistochemistry in assessing its aggressiveness and understanding a novel aspect of its pathogenesis.


Assuntos
Abdome , Adenocarcinoma/diagnóstico , Neoplasias Pulmonares/secundário , Neoplasias Maxilares/diagnóstico , Palato Mole/patologia , Neoplasias das Glândulas Salivares/diagnóstico , Glândulas Salivares Menores/patologia , Adenocarcinoma/sangue , Adenocarcinoma/imunologia , Adenocarcinoma/patologia , Idoso , Biomarcadores/sangue , Transformação Celular Neoplásica , Feminino , Humanos , Imuno-Histoquímica , Neoplasias Maxilares/sangue , Neoplasias Maxilares/imunologia , Neoplasias Maxilares/patologia , Proteínas Proto-Oncogênicas c-kit/sangue , Neoplasias das Glândulas Salivares/sangue , Neoplasias das Glândulas Salivares/imunologia , Neoplasias das Glândulas Salivares/patologia
17.
Med Princ Pract ; 22(1): 59-64, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-22738870

RESUMO

OBJECTIVE: To detect immunohistochemically the N-cadherin expression in different types of benign and malignant salivary gland tumors in an attempt to note any possible correlation to their development, stage and invasive properties. MATERIALS AND METHODS: N-cadherin expression was examined in tissue specimens from 49 salivary gland tumors including: pleomorphic adenomas (4), Warthin's tumors (10), and myoepitheliomas (4) (benign tumors), as well as adenoid cystic carcinomas (14), mucoepidermoid carcinomas (4), polymorphous low-grade adenocarcinomas (6), and adenocarcinomas not otherwise specified (5) (malignant tumors). Twelve specimens of normal salivary glands were used as control. The perineural invasion and stage of malignant salivary gland tumors were evaluated. Immunohistochemical procedure was performed automatically using the Bond Polymer Refine Detection Kit. RESULTS: N-cadherin expression was not found in normal salivary glands. In benign salivary gland tumors, N-cadherin along membranes of neoplastic cells as well as in centrocytes of lymphoid germinal centers was seen in 1 and 4 cases of Warthin's tumors, respectively. Varied degree of N-cadherin expression was found in 13 (45%) cases of malignant salivary gland tumors. N-cadherin expression was significantly correlated with perineural invasion (χ(2) = 11.7, p < 0.0001), but not with stage of malignant salivary gland tumors. CONCLUSION: N-cadherin expression was observed in malignant salivary gland tumors and could be an indicator of potentially aggressive behavior. N-cadherin expression by tumor cells could be attributed to perineural invasion.


Assuntos
Caderinas/sangue , Neoplasias das Glândulas Salivares/sangue , Neoplasias das Glândulas Salivares/patologia , Biomarcadores Tumorais , Humanos
18.
DNA Cell Biol ; 31(4): 520-3, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21942441

RESUMO

Basic fibroblast growth factor (FGF2) is a well-known endothelial mitogen that regulates endothelial cell proliferation, migration, differentiation, and survival. In the present study, we investigated the levels of FGF2 and fibroblastic growth factor receptor 1 (FGFR1) in saliva and serum of patients with salivary gland tumors. Saliva and serum samples were collected from 43 patients with salivary gland tumors and 40 healthy volunteers. The FGF2 and FGFR1 concentrations in saliva and serum samples were measured by enzyme-linked immunosorbent assay. We found that the levels of FGF2 and FGFR1 in saliva and serum from patients with salivary gland tumors were significantly higher than those from healthy control subjects. These results suggest that salivary FGF2 and FGFR1 can be used as potential biomarkers in the diagnosis of salivary gland tumors.


Assuntos
Biomarcadores Tumorais/metabolismo , Fator 2 de Crescimento de Fibroblastos/metabolismo , Receptor Tipo 1 de Fator de Crescimento de Fibroblastos/metabolismo , Saliva/metabolismo , Neoplasias das Glândulas Salivares/metabolismo , Idoso , Biomarcadores Tumorais/sangue , Estudos de Casos e Controles , Feminino , Fator 2 de Crescimento de Fibroblastos/sangue , Humanos , Masculino , Pessoa de Meia-Idade , Curva ROC , Receptor Tipo 1 de Fator de Crescimento de Fibroblastos/sangue , Neoplasias das Glândulas Salivares/sangue
19.
Biomed Pharmacother ; 64(10): 654-8, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-20970300

RESUMO

Heparin has a potential value as therapeutic agents that block P-selectin-mediated cell adhesion and prevent tumor metastasis. However, the strong anticoagulant potency limits its applicability for the anti-metastasis activity. Carboxyl and sulfate groups of heparin are closely related to its anticoagulant activity, so seven kinds of heparin derivatives related to carboxyl and sulfate groups were prepared, and their effects on anti-metastasis as ligand antagonist of p-selectin were studied. The results showed that heparin, carboxyl reduction heparin, 2-O-desulfated heparin and N-desulfated- N-acetylated heparin could inhibit the adhesion of tumor cells to endothelial cells and platelets effectively as ligand antagonist of P-selectin.


Assuntos
Heparina/análogos & derivados , Heparina/farmacologia , Neoplasias Pulmonares/prevenção & controle , Neoplasias Pulmonares/secundário , Selectina-P/antagonistas & inibidores , Anticoagulantes/efeitos adversos , Anticoagulantes/metabolismo , Anticoagulantes/farmacologia , Plaquetas/efeitos dos fármacos , Plaquetas/metabolismo , Carcinoma Adenoide Cístico/sangue , Carcinoma Adenoide Cístico/tratamento farmacológico , Carcinoma Adenoide Cístico/patologia , Adesão Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Células Endoteliais/efeitos dos fármacos , Células Endoteliais/metabolismo , Humanos , Ligantes , Neoplasias Pulmonares/sangue , Neoplasias Pulmonares/patologia , Metástase Neoplásica , Selectina-P/metabolismo , Neoplasias das Glândulas Salivares/sangue , Neoplasias das Glândulas Salivares/tratamento farmacológico , Neoplasias das Glândulas Salivares/patologia
20.
Oral Dis ; 15(8): 570-2, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19563418

RESUMO

OBJECTIVE: To determine the levels of serum sCD44v6 in patients with oral cancer and evaluate the value of serum sCD44v6 in adjuvant diagnosis, staging and monitoring treatment response in these patients. MATERIALS AND METHODS: A total of 112 hospitalized patients with oral and maxillofacial malignancy and 28 healthy individuals were examined for serum sCD44v6 levels. Venous blood was collected from these patients and the healthy individuals. One week after treatment, venous blood was collected once again in 60 patients with oral and maxillofacial squamous cell carcinoma (OSCC). RESULTS: The sCD44v6 concentration was not significantly different between patients with oral and maxillofacial malignancy and control group (P > 0.05). The levels of serum sCD44v6 in patients with OSCC and salivary carcinoma showed no difference with those in control group (P > 0.05). The sCD44v6 level in patients with stage III and IV disease was higher than that of patients with stage I and II and that of the control group, but the difference was not significant (P > 0.05). Serum sCD44v6 levels in patients with OSCC after treatment became lower than that prevailed during pretreatment (P < 0.05). CONCLUSION: The possible roles of CD44v6 in the diagnosis of oral and maxillofacial malignancy deserve further elucidation and evaluation. Serum sCD44v6 may be a valuable marker in monitoring treatment response in patients with OSCC.


Assuntos
Biomarcadores Tumorais/sangue , Carcinoma de Células Escamosas/sangue , Neoplasias de Cabeça e Pescoço/sangue , Receptores de Hialuronatos/sangue , Neoplasias Bucais/sangue , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma Adenoide Cístico/sangue , Carcinoma Adenoide Cístico/tratamento farmacológico , Carcinoma Adenoide Cístico/cirurgia , Carcinoma Mucoepidermoide/sangue , Carcinoma Mucoepidermoide/tratamento farmacológico , Carcinoma Mucoepidermoide/cirurgia , Carcinoma de Células Escamosas/tratamento farmacológico , Carcinoma de Células Escamosas/cirurgia , Estudos de Casos e Controles , Feminino , Neoplasias de Cabeça e Pescoço/tratamento farmacológico , Neoplasias de Cabeça e Pescoço/cirurgia , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias Bucais/tratamento farmacológico , Neoplasias Bucais/cirurgia , Estadiamento de Neoplasias , Valores de Referência , Neoplasias das Glândulas Salivares/sangue , Neoplasias das Glândulas Salivares/tratamento farmacológico , Neoplasias das Glândulas Salivares/cirurgia , Resultado do Tratamento
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