RESUMO
A cornea is innervated by sensory nerves, which branch into thick trunks, subbasal plexuses, and sensory endings. Appropriate assessment of nerve structure in a tissue provides a more complete understanding of the role of nerves in health and disease. Here, we present a whole-mount immunohistochemistry protocol that facilitates evaluation of nerve architecture throughout the mouse cornea. The fixation step in this protocol allows for reliable detection of nerve structures within the cornea and likely other tissues. For complete details on the use and execution of this protocol, please refer to Yun et al, (2020).
Assuntos
Córnea , Imuno-Histoquímica/métodos , Nervo Oftálmico , Animais , Córnea/anatomia & histologia , Córnea/inervação , Dissecação , Feminino , Masculino , Camundongos , Nervo Oftálmico/anatomia & histologia , Nervo Oftálmico/química , Nervo Oftálmico/citologiaRESUMO
The distribution of the postganglionic parasympathetic fibers originating in the pterygopalatine ganglion (PTPG) has been traced in the guinea pig by means of the HRP and WGA-HRP methods. The greatest number of labeled cells were observed when WGA-HRP was injected in the lacrimal gland. After applying HRP to all the ramifications of the maxillary and ophthalmic divisions of the trigeminal nerve, labeled neurons were found in the PTPG. Numerous PTPG fibers were detected in the ethmoidal and sphenopalatine nerves. The presence of PTPG fibers in the supraorbital, infratrochlear, zygomaticotemporal, zygomaticofacial-inferior palpebral, sphenopalatine and infraorbital-superior alveolar nerves has not hitherto been reported in mammals.
