RESUMO
We investigated the hibernation effect of epigallocatechin-3-O-gallate (EGCG) on neonatal human tarsal fibroblasts (nHTFs) by analyzing the expression of cell cycle-related genes. EGCG application to culture media moderately inhibited the growth of nHTFs, and the removal of EGCG from culture media led to complete recovery of cell growth. EGCG resulted in a slight decrease in the cell population of the S and G(2)/M phases of cell cycle with concomitant increase in that of the G(0)/G(1) phase, but this cell cycle profile was restored to the initial level after EGCG removal. The expression of cyclin D1 (CCND1), CCNE2, CCN-dependent kinase 6 (CDK6), and CDK2 was restored, whereas that of CCNA, CCNB1, and CDK1 was irreversibly attenuated. The expression of a substantial number of genes analyzed by cDNA microarray was affected by EGCG application, and these affected expression levels were restored to the normal levels after EGCG removal. We also found the incorporation of FITC-EGCG into the cytosol of nHTFs and its further nuclear translocation, which might lead to the regulation of the exogenous signals directed to genes for cellular responses including proliferation and cell cycle progression. These results suggest that EGCG temporarily affects not only genes related to the cell cycle but also various other cellular functions.
Assuntos
Catequina/análogos & derivados , Proteínas de Ciclo Celular/metabolismo , Fibroblastos/efeitos dos fármacos , Ossos do Tarso/citologia , Catequina/farmacologia , Ciclo Celular , Proteínas de Ciclo Celular/genética , Proliferação de Células , Células Cultivadas , Fibroblastos/citologia , Fibroblastos/metabolismo , Regulação da Expressão Gênica , Humanos , Lactente , Masculino , Análise de Sequência com Séries de OligonucleotídeosRESUMO
No disponible
Assuntos
Adulto , Masculino , Humanos , Tumor Glômico/diagnóstico , Neoplasias Cutâneas/diagnóstico , Tumor Glômico/cirurgia , Tumor Glômico/patologia , Mãos/patologia , Nádegas/patologia , Neoplasias Cutâneas/cirurgia , Neoplasias Cutâneas/patologia , Ossos do Tarso/citologiaRESUMO
Las células oxinticopépticas de la glándula proventricular profunda en el cardenal rojo (paroaria gularis gularis) fueron estudiadas por medio de la microscopía electrónica de transmisión. Estas células tienen la estructura fina típica de una cimogénica (proteína) y célula hidroclórica ácido/secretora (parecido a esos hallazgos se han encontrado en las células parietales y principales de mamíferos y en las células oxínticas de anfíbios): la presencia de numerosas mitocondrias, desarrollando retículo endoplásmico liso y granuloso y gránulos de secreción
Assuntos
Animais , Células Parietais Gástricas/ultraestrutura , Estômago de Aves/ultraestrutura , Proventrículo/citologia , Brasil , Estômago de Aves/citologia , Ácido Gástrico , Microscopia Eletrônica , Mitocôndrias/ultraestrutura , Proventrículo/ultraestrutura , Ossos do Tarso/citologia , Ossos do Tarso/ultraestruturaRESUMO
Disaggregated chondrocytes from embryonic chick whole sterna proliferate in three-dimensional collagen gels forming mixtures of cartilage nodules (chondroids) and columns of cells. A typical matrix elaborated by the cells is composed of collagen type II and chondroitin and keratan sulphate proteoglycans. The cells of the chondroids are small and uniform whilst those in the columns are larger (hypertrophic) and have a vacuolated cytoplasm. Disaggregated cells from the caudal portion of the sternum (which in vivo contains small uniform cells and does not calcify) tend to form chondroids in culture whilst cells from the cephalic portion (which in vivo contains large hypertrophic cells and whose matrix calcifies) tend to form columns of hypertrophic cells. Only the cultures containing hypertrophic cells synthesise collagen type X and only the hypertrophic cells contain alkaline phosphatase. Collagen type X and alkaline phosphatase may therefore be coregulators of the calcification of hypertrophic cartilage matrix.
