A stochastic model to investigate the effects of control strategies on calves exposed to Ostertagia ostertagi.

Predicting the effectiveness of parasite control strategies requires accounting for the responses of individual hosts and the epidemiology of parasite supra- and infra-populations. The first objective was to develop a stochastic model that predicted the parasitological interactions within a group of first season grazing calves challenged by Ostertagia ostertagi, by considering phenotypic variation amongst the calves and variation in parasite infra-population. Model behaviour was assessed using variations in parasite supra-population and calf stocking rate. The model showed the initial pasture infection level to have little impact on parasitological output traits, such as worm burdens and FEC, or overall performance of calves, whereas increasing stocking rate had a disproportionately large effect on both parasitological and performance traits. Model predictions were compared with published data taken from experiments on common control strategies, such as reducing stocking rates, the 'dose and move' strategy and strategic treatment with anthelmintic at specific times. Model predictions showed in most cases reasonable agreement with observations, supporting model robustness. The stochastic model developed is flexible, with the potential to predict the consequences of other nematode control strategies, such as targeted selective treatments on groups of grazing calves.

The reindeer abomasal nematode (Ostertagia gruehneri) is naturally transmitted to sheep when sharing pastures.

The increasing number of sheep (Ovis aries) in northern Finland, often alternately corralled with winter-fed reindeer (Rangifer tarandus tarandus), creates potential for cross-infection of gastrointestinal nematodes. The aim of this study was to elucidate this possibility with 43 animals. Eleven reindeer and 8 sheep had shared a corral by turns, reindeer during winters, and sheep in summers. Another 12 reindeer had no known contact with sheep. Twelve sheep had no close contact to other ruminants. Both reindeer groups were free-ranging during summers. During slaughter in September to November, 2003, abomasa and parts of intestines were collected. Gastrointestinal nematodes were counted and identified. The species found were the following: in reindeer, Ostertagia gruehneri/Ostertagia arctica, Mazamastrongylus dagestanica, Nematodirus tarandi, Nematodirella longissimespiculata and Bunostomum trigonocephalum; in sheep, Teladorsagia circumcincta/Teladorsagia trifurcata, O. gruehneri/O. arctica, Nematodirus filicollis and N. spathiger. In the sheep sharing corral with reindeer, the only abomasal nematode species found was O. gruehneri, a reindeer parasite. The generation interval of O. gruehneri in Finnish reindeer appears to be shorter than in Canadian Arctic caribou, where complete larval inhibition leading to only one generation yearly has been reported.

The establishment rate of a sheep nematode: revisiting classics using a meta-analysis of 87 experiments.

Strongyle nematode establishment rate in their host is a highly variable life history trait, which makes it difficult to estimate. A meta-analysis was applied to the nematode Teladorsagia circumcincta of sheep in order to acquire a general framework of the factors modulating this life trait. A linear model was built with individual data on 540 infected lambs extracted from 13 articles. Lambs breed and age, time lag between last infection and the interaction between infection mode, infective dose and the number of repeated infective doses were significantly related with the establishment rate. The influence of infection mode on nematode establishment rate was also evaluated by comparing nematode establishment rate distributions within lamb populations infected under different conditions. Natural and repeated experimental infections lead to similar distribution of establishment rate. Conversely, these infection conditions resulted in different parasite establishment rates in average (12.7 and 23.4%, respectively). Three hypotheses are discussed to explain this result: immune protective response, host avoidance behaviour and parasite virulence.

Inactivation of eggs and larvae of the cattle nematodes Ostertagia ostertagi and Cooperia oncophora after passage in pigs.

The study investigated the effect of gastrointestinal passage in pigs on free-living stages of bovine nematodes. Two Landrace x Yorkshire pigs, A and B, were fed fresh eggs of Ostertagia ostertagi and Cooperia oncophora while two other pigs, C and D, were fed third stage larvae (L3) of the same parasites. Faeces from the pigs were collected for 48 h after ingestion. In pigs A and B, 15 and 66% of the eggs were recovered after passage, respectively. However, only 0.003 and 0.002% of the ingested eggs developed into third stage larvae (L3) after subsequent culturing. In pigs C and D, 0.01 and 0.02% of the L3 survived the passage of the gastrointestinal tract. Fresh O. ostertagi and C. oncophora eggs were cultured in parasite free porcine and bovine faeces. Only 0.05% L3 developed in porcine faeces, whereas 21% of the eggs developed into L3 in the bovine culture. Our results demonstrate an extremely poor rate of development and survival of both bovine nematode eggs and infective larvae after passage in pigs. It may imply that pigs can play an important role in reducing transmission of cattle nematodes if the two species are grazed together or alternately.

Survival of infective Ostertagia ostertagi larvae on pasture plots under different simulated grazing conditions.

This study was carried out to examine the survival of infective Ostertagia ostertagi larvae (L(3)) on pasture under different simulated conditions of grazing, i.e. mixed grazing of cattle and nose-ringed sows, or grazing by cattle alone. Standardised pats of cattle faeces containing O. ostertagi eggs were deposited on three types of herbage plots, which were divided into zone 1: faecal pat; zone 2: a circle extending 25cm from the edge of the faecal pat; zone 3: a circle extending 25cm from the edge of zone 2. For "tall herbage" (TH) plots, the herbage in zone 2 was allowed to grow naturally, while the herbage in zone 3 was cut down to 5-7cm fortnightly, imitating a cattle-only pasture. For "short herbage" (SH) plots, the herbage in both zones 2 and 3 were cut down to 5-7cm fortnightly, imitating mixed grazing of cattle and sows. The grass in the "short herbage and scattered faeces" (SH/SF) plots were cut as for SH plots, and the faeces were broken down 3 weeks after deposition and scattered within zone 2, imitating the rooting behaviour of co-grazing sows. Five faecal pats from each plot group were collected on monthly basis, along with the herbage from zones 2 and 3 cut down to the ground. Infective larvae were then recovered from both faeces and herbage. The numbers of L(3) recovered from zone 1 were higher in the TH plots than in the other two groups and, furthermore, the larval counts from SH plots were always higher than from SH/SF plots. The three groups followed a similar pattern during the season regarding numbers of L(3) in zone 2, and no clear patterns between plot types were obtained. The presence of L(3) in zone 3 was almost negligible. Important differences were seen throughout the study from the biological point of view; more L(3) were able to survive in faeces on the TH plots, presumably reflecting a better protection from heat and desiccation compared to those in the other plots. The overall results support the idea that mixed grazing of cattle and pigs favour the reduction of O. ostertagi larval levels in pasture. This reduction is mainly due to the grazing behaviour of pigs, which by grazing up to the very edge of the cattle faeces, will either expose the larvae in faeces to adverse environmental summer conditions or ingest cattle parasite larvae, or both.

Epidemiology of Ostertagia in the Northwestern USA.

The two most significant environmental factors that influence the epidemiology of Ostertagia are temperature and moisture. These factors vary more in the west and northwest than in any other part of the USA because of extreme regional differences in climate, topography, and land use. Consequently, patterns of Ostertagia transmission and inhibition also vary widely from region to region and from year to year. Recent data require revisions of previously accepted concepts of northern/southern transmission and inhibition. In particular, the flaw of liberal extrapolation of information from one region to another has now been recognized. Inhibition may not occur along the coast of the Pacific Northwest. Although not completely delineated, the transition zone between northern and southern patterns of inhibition appears to be significantly wider and more variable than previously recognized; Oregon data suggest a possible indeterminate and variable band extending north of the 45th parallel and south of the 43rd parallel. Summer inhibition has now been documented in areas of Oregon and Montana. Other than the recent Oregon and Montana studies, data on this transition zone in the Rocky Mountain region and high plains are sparse to non-existent. In contrast to previously accepted doctrine, the fall rise in egg count generally exceeds the traditionally accepted spring rise in many areas. From the Pacific Northwest to the Midwest, year-round transmission patterns have been observed. The presence of geothermal ground water sources produces microclimates that favor larval survival in many areas.(ABSTRACT TRUNCATED AT 250 WORDS)

The effects of late turnout on the epidemiology and control of ostertagiasis in calves.

The main purpose of this experiment was to study the importance of time of turnout on the epidemiology and impact of ostertagiasis in calves pastured for the first time. When time of turnout onto permanent pasture was postponed from mid-May to mid-June, fewer overwintered larvae were picked up and re-contamination of pastures was markedly diminished. Consequently, the acquisition of loss-producing parasite burdens in the late part of the season was significantly reduced. Late turnout as a control strategy is discussed in relation to other preventive measures.

Estimation of uptake of digestive tract strongyle larvae from pasture, using oesophagus fistulated sheep.

Three Merino of Arles ewes fistulated at the oesophagus were used in autumn and spring in order to assess their larval uptake on infected irrigated pasture. The rate of transmission (ingested/available larvae of digestive tract strongyle) was low when the pasture was heavily infected; it was not otherwise modified by environmental factors.

The infectivity of some nematode parasites of reindeer (Rangifer tarandus L.) and elk (Alces alces L.) for cattle and sheep.

Cross infections were carried out by dosing parasite-free calves and lambs with third stage nematode larvae cultured from the faeces of reindeer and elk. Two lambs each received 20,000 larvae from reindeer, and one lamb 20,000 larvae from elk. One calf received 50,000 larvae from reindeer and one 50,000 from elk. The animals were killed 28 days after infection. Egg output did not differ significantly between the lambs nor between the calves, but in the lambs the output was much higher than in the calves (highest cound 4300 versus 275). Worm counts varied among the animals independent of the origin of the larvae. The highest take was found in the lamb infected with larvae from elk (81.5%) and the lowest in the calf infected with larvae from elk (15.1%). Ostertagia leptospicularis was the dominant species (80% or more) and the only other species present were Skrjabinagia kolchida and Spiculopteragia boehmi. The latter was found in a higher percentage (14.0 and 10.4) in the animals that received larvae cultured from elk faeces. Under natural conditions O. leptospicularis is possibly the only pathogenic species that can play a role in cross contamination to cattle and sheep.

Seasonal transmission of bovine gastrointestinal nematodes in the Texas Gulf Coast.

Sentinel calves were placed in pastures for 1 month in two environmentally dissimilar areas of the Texas Gulf Coast to determine the seasonal transmission of various gastrointestinal nematodes. Transmission was determined for Cooperia spp, Haemonchus placei, Ostertagia ostertagi, and Trichostrongylus axei. Large numbers of Cooperia spp were acquired from May through November, with the peak of transmission occurring in July and August. Haemonchus placei was encountered on both field sites but was transmitted in large numbers only at one site, during August. Ostertagia ostertagi was acquired primarily from November through May, with the peak of transmission occurring in January and February. January through March was the period when the greatest numbers of Ostertagia larvae undergoing arrested development were acquired. Trichostrongylus axei was abundance in December and January at one field site. In general, trends of transmission were the same in both areas, indicating that weather conditions were most important than vegetation type in larval transmission.