RESUMO
A study was made of the effect of different doses of hydroxythiamine on activity of alkaline phosphatase and transketolase in rat spleen and thymus during primary and secondary administration of human gamma-globulin. Immunization of rats significantly raised alkaline phosphatase activity in both organs. Administration of a large single dose of hydroxythiamine (400 mg/kg bw) reduced transketolase activity in the thymus and spleen in both immunized and non-immunized animals and did not change alkaline phosphatase activity in these organs.
Assuntos
Fosfatase Alcalina/metabolismo , Formação de Anticorpos/efeitos dos fármacos , Tecido Linfoide/enzimologia , Oxitiamina/imunologia , Deficiência de Tiamina/enzimologia , Tiazóis/imunologia , Transcetolase/metabolismo , Animais , Imunização , Tecido Linfoide/imunologia , Masculino , Tamanho do Órgão/efeitos dos fármacos , Ratos , Deficiência de Tiamina/induzido quimicamente , Deficiência de Tiamina/imunologia , Fatores de Tempo , gama-Globulinas/administração & dosagemRESUMO
The effect of thiamin deficiency on the immune response and activity of some mechanisms of natural immunity was studied in experimental mature rats. Thiamin deficiency was simulated by a single injection of hydroxythiamin (thiamin antagonists). Hydroxythiamin markedly decreased the complement activity, phagocytic activity of peripheral blood leucocytes, serum bactericidal activity as well as antibody production in response to sheep red blood cells. On the contrary, lysozyme activity increased. Vitamin B1 deficiency reduced 14C-leucine incorporation activity in the liver proteins.